WO2003077843A3 - Methodes de purification de proteines de la famille de l'antigene carcinoembryonnaire (ace) - Google Patents

Methodes de purification de proteines de la famille de l'antigene carcinoembryonnaire (ace) Download PDF

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Publication number
WO2003077843A3
WO2003077843A3 PCT/US2003/007051 US0307051W WO03077843A3 WO 2003077843 A3 WO2003077843 A3 WO 2003077843A3 US 0307051 W US0307051 W US 0307051W WO 03077843 A3 WO03077843 A3 WO 03077843A3
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WO
WIPO (PCT)
Prior art keywords
family member
substantially free
member proteins
disclosed
cea
Prior art date
Application number
PCT/US2003/007051
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English (en)
Other versions
WO2003077843A2 (fr
Inventor
Diane Newman
Cathy Landmann
Mark Colgin
Original Assignee
Aspenbio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aspenbio Inc filed Critical Aspenbio Inc
Priority to AU2003218007A priority Critical patent/AU2003218007A1/en
Publication of WO2003077843A2 publication Critical patent/WO2003077843A2/fr
Publication of WO2003077843A3 publication Critical patent/WO2003077843A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne des méthodes améliorées destinées à la purification de protéines de la famille de l'antigène carcinoembryonnaire (ACE). Ces méthodes comprennent des étapes de chromatographie d'échange cationique à un pH inférieur à 4,0 et de chromatographie d'exclusion diffusion, mais ne comprend pas d'étapes faisant intervenir de l'acide perchlorique ou d'affinité envers les anticorps. Les protéines purifiées résultantes sont pures à 90 % au moins, sensiblement exemptes d'antigènes de réaction croisée, sensiblement exemptes de CA19-9, sensiblement exemptes d'endotoxines et sensiblement exemptes d'anticorps. Les méthodes de l'invention permettent d'obtenir des puretés supérieures à 98 %. L'invention concerne également l'utilisation de protéines purifiées de la famille de l'antigène carcinoembryonnaire (ACE) comme étalons de référence, dans des excipients pharmaceutiques, et leur préparation sous forme de vaccins. L'invention concerne également des compositions, la purification et l'utilisation de protéines de la famille de l'antigène carcinoembryonnaire (ACE) renfermant des épitopes immunogènes modifiés.
PCT/US2003/007051 2002-03-13 2003-03-06 Methodes de purification de proteines de la famille de l'antigene carcinoembryonnaire (ace) WO2003077843A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003218007A AU2003218007A1 (en) 2002-03-13 2003-03-06 Methods for purifying selected cea family member proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/097,447 2002-03-13
US10/097,447 US6949629B2 (en) 2002-03-13 2002-03-13 Methods for purifying selected CEA family member proteins

Publications (2)

Publication Number Publication Date
WO2003077843A2 WO2003077843A2 (fr) 2003-09-25
WO2003077843A3 true WO2003077843A3 (fr) 2006-03-16

Family

ID=28039186

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/007051 WO2003077843A2 (fr) 2002-03-13 2003-03-06 Methodes de purification de proteines de la famille de l'antigene carcinoembryonnaire (ace)

Country Status (3)

Country Link
US (1) US6949629B2 (fr)
AU (1) AU2003218007A1 (fr)
WO (1) WO2003077843A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1417965A1 (fr) * 2002-11-07 2004-05-12 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Molécules capables de se lier à des lectines de type-C, leur identification et usages
US8541187B2 (en) 2011-03-01 2013-09-24 Bio Tracking, LLC Systems and methods for detecting animal pregnancy
GB201208756D0 (en) * 2012-05-17 2012-07-04 Medical Res Council Methods
CN112014198B (zh) * 2020-06-09 2021-11-09 中国科学院生物物理研究所 一种用于富集内源性低分子量蛋白和多肽的试剂盒及方法

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Also Published As

Publication number Publication date
AU2003218007A1 (en) 2003-09-29
AU2003218007A8 (en) 2003-09-29
US20030176646A1 (en) 2003-09-18
US6949629B2 (en) 2005-09-27
WO2003077843A2 (fr) 2003-09-25

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