WO2003077643A3 - Methods for increasing the oil content in plants by reducing the content of one or more storage proteins - Google Patents

Methods for increasing the oil content in plants by reducing the content of one or more storage proteins Download PDF

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Publication number
WO2003077643A3
WO2003077643A3 PCT/EP2003/002733 EP0302733W WO03077643A3 WO 2003077643 A3 WO2003077643 A3 WO 2003077643A3 EP 0302733 W EP0302733 W EP 0302733W WO 03077643 A3 WO03077643 A3 WO 03077643A3
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WO
WIPO (PCT)
Prior art keywords
content
plants
increasing
reducing
storage proteins
Prior art date
Application number
PCT/EP2003/002733
Other languages
German (de)
French (fr)
Other versions
WO2003077643A2 (en
Inventor
Joerg Bauer
Original Assignee
Basf Plant Science Gmbh
Joerg Bauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh, Joerg Bauer filed Critical Basf Plant Science Gmbh
Priority to AU2003209737A priority Critical patent/AU2003209737A1/en
Publication of WO2003077643A2 publication Critical patent/WO2003077643A2/en
Publication of WO2003077643A3 publication Critical patent/WO2003077643A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06Fungi, e.g. yeasts
    • A61K36/062Ascomycota
    • A61K36/064Saccharomycetales, e.g. baker's yeast
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/286Carthamus (distaff thistle)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/31Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/52Juglandaceae (Walnut family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/63Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/899Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medical Informatics (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Edible Oils And Fats (AREA)

Abstract

The invention relates to methods for increasing the oil content in plants by reducing the content of storage proteins by one or more. The invention also relates to the use of plants having a reduced content of storage proteins for producing foodstuffs, feedstuffs, seeds, pharmaceutics or fine chemicals, particularly for producing oils.
PCT/EP2003/002733 2002-03-20 2003-03-17 Methods for increasing the oil content in plants by reducing the content of one or more storage proteins WO2003077643A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003209737A AU2003209737A1 (en) 2002-03-20 2003-03-17 Methods for increasing the oil content in plants by reducing the content of one or more storage proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2002112893 DE10212893A1 (en) 2002-03-20 2002-03-20 Process for increasing the oil content in plants
DE10212893.6 2002-03-20

Publications (2)

Publication Number Publication Date
WO2003077643A2 WO2003077643A2 (en) 2003-09-25
WO2003077643A3 true WO2003077643A3 (en) 2004-01-08

Family

ID=27815874

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/002733 WO2003077643A2 (en) 2002-03-20 2003-03-17 Methods for increasing the oil content in plants by reducing the content of one or more storage proteins

Country Status (3)

Country Link
AU (1) AU2003209737A1 (en)
DE (1) DE10212893A1 (en)
WO (1) WO2003077643A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
AR047598A1 (en) * 2004-02-10 2006-01-25 Monsanto Technology Llc TRANSGENIZED CORN SEED WITH GREATER AMINO ACID CONTENT
CN101128588A (en) 2004-08-11 2008-02-20 孟山都技术有限公司 Enhanced zein reduction in transgenic corn seed
CN101600798A (en) 2006-08-31 2009-12-09 孟山都技术有限公司 Phased small RNAs
JP6034053B2 (en) * 2012-04-26 2016-11-30 トヨタ自動車株式会社 Production method of total plant seeds

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0787801A2 (en) * 1996-02-01 1997-08-06 Mitsubishi Corporation Method for increasing storage lipid content in plant seed
WO1998026064A2 (en) * 1996-12-09 1998-06-18 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
WO1999053050A1 (en) * 1998-04-08 1999-10-21 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
WO2002000904A2 (en) * 2000-06-23 2002-01-03 E. I. Du Pont De Nemours And Company Recombinant constructs and their use in reducing gene expression

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0620281A3 (en) * 1993-03-31 1995-05-03 Mitsubishi Corp Oilseed crops producing valuable seeds having altered amino acid composition and fatty acid composition.
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0787801A2 (en) * 1996-02-01 1997-08-06 Mitsubishi Corporation Method for increasing storage lipid content in plant seed
WO1998026064A2 (en) * 1996-12-09 1998-06-18 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
WO1999053050A1 (en) * 1998-04-08 1999-10-21 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
WO2002000904A2 (en) * 2000-06-23 2002-01-03 E. I. Du Pont De Nemours And Company Recombinant constructs and their use in reducing gene expression

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAMMOND S M ET AL: "POST-TRANSCRIPTIONAL GENE SILENCING BY DOUBLE-STRANDED RNA", NATURE REVIEWS GENETICS, MACMILLAN MAGAZINES, GB, vol. 2, no. 2, February 2001 (2001-02-01), pages 110 - 119, XP001146153 *
MATZKE M A ET AL: "Transgene silencing by the host genome defense: Implications for the evolution of epigenetic control mechanisms in plants and vertebrates", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 43, no. 2-3, June 2000 (2000-06-01), pages 401 - 415, XP002189062, ISSN: 0167-4412 *
WESLEY S VARSHA ET AL: "Construct design for efficient, effective and high-throughput gene silencing in plants", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 27, no. 6, September 2001 (2001-09-01), pages 581 - 590, XP002187670, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
DE10212893A9 (en) 2004-09-09
AU2003209737A1 (en) 2003-09-29
WO2003077643A2 (en) 2003-09-25
DE10212893A1 (en) 2003-10-16
AU2003209737A8 (en) 2003-09-29

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