WO2003072814A3 - Improved method for allele-specific pcr - Google Patents

Improved method for allele-specific pcr Download PDF

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Publication number
WO2003072814A3
WO2003072814A3 PCT/EP2003/001725 EP0301725W WO03072814A3 WO 2003072814 A3 WO2003072814 A3 WO 2003072814A3 EP 0301725 W EP0301725 W EP 0301725W WO 03072814 A3 WO03072814 A3 WO 03072814A3
Authority
WO
WIPO (PCT)
Prior art keywords
primer
discriminating
sequence variant
proxi
complementary
Prior art date
Application number
PCT/EP2003/001725
Other languages
English (en)
French (fr)
Other versions
WO2003072814A2 (en
Inventor
Alfred Pingoud
Meinhard Hahn
Bjoern Tews
Jochen Wilhelm
Peter Friedhoff
Andreas Marx
Michael Strerath
Daniel Summerer
Original Assignee
Roche Diagnostics Gmbh
Hoffmann La Roche
Alfred Pingoud
Meinhard Hahn
Bjoern Tews
Jochen Wilhelm
Peter Friedhoff
Andreas Marx
Michael Strerath
Daniel Summerer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Hoffmann La Roche, Alfred Pingoud, Meinhard Hahn, Bjoern Tews, Jochen Wilhelm, Peter Friedhoff, Andreas Marx, Michael Strerath, Daniel Summerer filed Critical Roche Diagnostics Gmbh
Priority to AU2003229549A priority Critical patent/AU2003229549A1/en
Publication of WO2003072814A2 publication Critical patent/WO2003072814A2/en
Publication of WO2003072814A3 publication Critical patent/WO2003072814A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PCT/EP2003/001725 2002-02-26 2003-02-20 Improved method for allele-specific pcr WO2003072814A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003229549A AU2003229549A1 (en) 2002-02-26 2003-02-20 Improved method for allele-specific pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02004221.4 2002-02-26
EP02004221 2002-02-26

Publications (2)

Publication Number Publication Date
WO2003072814A2 WO2003072814A2 (en) 2003-09-04
WO2003072814A3 true WO2003072814A3 (en) 2003-12-18

Family

ID=27763335

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001725 WO2003072814A2 (en) 2002-02-26 2003-02-20 Improved method for allele-specific pcr

Country Status (2)

Country Link
AU (1) AU2003229549A1 (sv)
WO (1) WO2003072814A2 (sv)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008070370A2 (en) 2006-11-02 2008-06-12 University Of Utah Research Foundation Oligonucleotides for use in allele-specific pcr
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
WO2011104695A2 (en) * 2010-02-26 2011-09-01 GAMMAGENETICS Sàrl Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5750343A (en) * 1993-12-09 1998-05-12 Syntex Inc. Methods of detecting nucleic acids with nucleotide probes containing 4'-substituted nucleotides and kits therefor
WO1999040219A1 (en) * 1998-02-05 1999-08-12 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5750343A (en) * 1993-12-09 1998-05-12 Syntex Inc. Methods of detecting nucleic acids with nucleotide probes containing 4'-substituted nucleotides and kits therefor
WO1999040219A1 (en) * 1998-02-05 1999-08-12 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SUMMERER D AND MARX A: "Differential minor groove interactions between DNA polymerase and sugar backbone of primer and template strands", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 124, no. 6, 13 February 2002 (2002-02-13), pages 910 - 911, XP002213302 *
SUMMERER D UND MARX A: "DNA polymerase selectivity: sugar interactions monitored with high-fidelity nucleotides", ANGEWANDTE CHEMIE, vol. 40, no. 19, May 2001 (2001-05-01), pages 3693 - 3695, XP002213303 *

Also Published As

Publication number Publication date
WO2003072814A2 (en) 2003-09-04
AU2003229549A1 (en) 2003-09-09

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