WO2003070197A3 - RNA INTERFERENCE MEDIATED INHIBITION OF TGF-BETA AND TGF-BETA RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) - Google Patents

RNA INTERFERENCE MEDIATED INHIBITION OF TGF-BETA AND TGF-BETA RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) Download PDF

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Publication number
WO2003070197A3
WO2003070197A3 PCT/US2003/007273 US0307273W WO03070197A3 WO 2003070197 A3 WO2003070197 A3 WO 2003070197A3 US 0307273 W US0307273 W US 0307273W WO 03070197 A3 WO03070197 A3 WO 03070197A3
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Prior art keywords
tgf
beta
nucleic acid
sina
gene expression
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PCT/US2003/007273
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French (fr)
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WO2003070197A2 (en
Inventor
James Mcswiggen
Leonid Beigelman
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Sirna Therapeutics Inc
James Mcswiggen
Leonid Beigelman
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Publication date
Application filed by Sirna Therapeutics Inc, James Mcswiggen, Leonid Beigelman filed Critical Sirna Therapeutics Inc
Priority to EP03716430A priority Critical patent/EP1499631A4/en
Priority to AU2003220136A priority patent/AU2003220136A1/en
Priority to JP2003569157A priority patent/JP2005517423A/en
Publication of WO2003070197A2 publication Critical patent/WO2003070197A2/en
Priority to US10/923,475 priority patent/US20050227936A1/en
Publication of WO2003070197A3 publication Critical patent/WO2003070197A3/en
Priority to US11/054,047 priority patent/US20050287128A1/en

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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
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Abstract

The present invention concerns methods and reagents useful in modulating transforming growth factor beta (TGF-beta) and transforming growth factor beta receptor (TGF-betaR) gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating RNA interference (RNAi) against transforming growth factor beta (TGF-beta) and transforming growth factor beta receptor (TGF-betaR) gene expression and/or activity. The siNA molecules are useful in the treatment and diagnosis of conditions that respond to the modulation of TGF-beta and/or TGF-betaR expression or activity.
PCT/US2003/007273 2001-05-18 2003-02-11 RNA INTERFERENCE MEDIATED INHIBITION OF TGF-BETA AND TGF-BETA RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) WO2003070197A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP03716430A EP1499631A4 (en) 2002-02-20 2003-02-11 Rna interference mediated inhibition of tgf-beta and tgf-beta receptor gene expression using short interfering nucleic acid (sina)
AU2003220136A AU2003220136A1 (en) 2002-02-20 2003-02-11 RNA INTERFERENCE MEDIATED INHIBITION OF TGF-BETA AND TGF-BETA RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
JP2003569157A JP2005517423A (en) 2002-02-20 2003-02-11 RNA interference-mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acids (siNA)
US10/923,475 US20050227936A1 (en) 2001-05-18 2004-08-20 RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA)
US11/054,047 US20050287128A1 (en) 2001-05-18 2005-02-09 RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA)

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US35858002P 2002-02-20 2002-02-20
US60/358,580 2002-02-20
US36312402P 2002-03-11 2002-03-11
US60/363,124 2002-03-11
US38678202P 2002-06-06 2002-06-06
US60/386,782 2002-06-06
US40678402P 2002-08-29 2002-08-29
US60/406,784 2002-08-29
US40837802P 2002-09-05 2002-09-05
US60/408,378 2002-09-05
US40929302P 2002-09-09 2002-09-09
US60/409,293 2002-09-09
US42555902P 2002-11-12 2002-11-12
US60/425,559 2002-11-12
US44012903P 2003-01-15 2003-01-15
US60/440,129 2003-01-15

Related Child Applications (1)

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US10/923,475 Continuation-In-Part US20050227936A1 (en) 2001-05-18 2004-08-20 RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA)

Publications (2)

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WO2003070197A2 WO2003070197A2 (en) 2003-08-28
WO2003070197A3 true WO2003070197A3 (en) 2004-11-25

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PCT/US2003/007273 WO2003070197A2 (en) 2001-05-18 2003-02-11 RNA INTERFERENCE MEDIATED INHIBITION OF TGF-BETA AND TGF-BETA RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)

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EP (1) EP1499631A4 (en)
JP (1) JP2005517423A (en)
AU (1) AU2003220136A1 (en)
WO (1) WO2003070197A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
WO2005019422A2 (en) 2003-08-13 2005-03-03 The Board Of Trustees Of The University Of Illinois Silencing of tgf-beta receptor type ii expression by sirna
EP1758998B1 (en) * 2004-01-30 2010-12-15 Quark Pharmaceuticals, Inc. Oligoribonucleotides and methods of use thereof for treatment of fibrotic conditions and other diseases
DE102004025881A1 (en) 2004-05-19 2006-01-05 Beiersdorf Ag Oligoribonucleotides for influencing hair growth
JP5296328B2 (en) * 2007-05-09 2013-09-25 独立行政法人理化学研究所 Single-stranded circular RNA and method for producing the same
EP3372684B1 (en) 2010-08-24 2020-10-07 Sirna Therapeutics, Inc. Single-stranded rnai agents containing an internal, non-nucleic acid spacer
WO2012058210A1 (en) 2010-10-29 2012-05-03 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACIDS (siNA)
ES2808862T3 (en) * 2013-03-27 2021-03-02 Isarna Therapeutics Gmbh Modified TGF-beta oligonucleotides

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna
WO2000044914A1 (en) * 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna
WO2001036646A1 (en) * 1999-11-19 2001-05-25 Cancer Research Ventures Limited Inhibiting gene expression with dsrna

Family Cites Families (2)

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Publication number Priority date Publication date Assignee Title
DE19956568A1 (en) * 1999-01-30 2000-08-17 Roland Kreutzer Method and medicament for inhibiting the expression of a given gene
TR200401292T3 (en) * 2000-12-01 2004-07-21 Max@Planck@Gesellschaft�Zur�F�Rderung�Der�Wissenschaften the rnaágirişimineáyoláaçanáküçükárnaámolekül

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna
WO2000044914A1 (en) * 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna
WO2001036646A1 (en) * 1999-11-19 2001-05-25 Cancer Research Ventures Limited Inhibiting gene expression with dsrna

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ELBASHIR ET AL.: "Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells", NATURE, vol. 411, 24 May 2001 (2001-05-24), pages 494 - 498, XP002206451 *
See also references of EP1499631A4 *
VARAMBALLY ET AL.: "The polycomb group protein EZH2 is involved in progression of prostate cancer", NATURE, vol. 419, 10 October 2002 (2002-10-10), pages 624 - 629, XP002969193 *

Also Published As

Publication number Publication date
AU2003220136A1 (en) 2003-09-09
EP1499631A2 (en) 2005-01-26
EP1499631A4 (en) 2006-01-25
JP2005517423A (en) 2005-06-16
WO2003070197A2 (en) 2003-08-28
AU2003220136A8 (en) 2003-09-09

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