WO2003068981A3 - Verfahren zur reduktion der hintergrund-kontamination nach markierungsreaktionen - Google Patents

Verfahren zur reduktion der hintergrund-kontamination nach markierungsreaktionen Download PDF

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Publication number
WO2003068981A3
WO2003068981A3 PCT/EP2003/001590 EP0301590W WO03068981A3 WO 2003068981 A3 WO2003068981 A3 WO 2003068981A3 EP 0301590 W EP0301590 W EP 0301590W WO 03068981 A3 WO03068981 A3 WO 03068981A3
Authority
WO
WIPO (PCT)
Prior art keywords
background contamination
reducing background
marking
reactions
marking reactions
Prior art date
Application number
PCT/EP2003/001590
Other languages
English (en)
French (fr)
Other versions
WO2003068981A2 (de
Inventor
Christian Korfhage
Original Assignee
Qiagen Gmbh
Christian Korfhage
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh, Christian Korfhage filed Critical Qiagen Gmbh
Priority to EP03739498A priority Critical patent/EP1476579A2/de
Publication of WO2003068981A2 publication Critical patent/WO2003068981A2/de
Publication of WO2003068981A3 publication Critical patent/WO2003068981A3/de
Priority to US10/918,975 priority patent/US20050064481A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Reduktion der Hintergrund-Kontamination nach einer Markierungsreaktion.
PCT/EP2003/001590 2002-02-15 2003-02-17 Verfahren zur reduktion der hintergrund-kontamination nach markierungsreaktionen WO2003068981A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03739498A EP1476579A2 (de) 2002-02-15 2003-02-17 Verfahren zur reduktion der hintergrund-kontamination nach markierungsreaktionen
US10/918,975 US20050064481A1 (en) 2002-02-15 2004-08-16 Method for reducing background contamination

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10206616.7 2002-02-15
DE10206616A DE10206616A1 (de) 2002-02-15 2002-02-15 Verfahren zur Reduktion der Background-Kontamination nach Markierungsreaktionen

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/918,975 Continuation-In-Part US20050064481A1 (en) 2002-02-15 2004-08-16 Method for reducing background contamination

Publications (2)

Publication Number Publication Date
WO2003068981A2 WO2003068981A2 (de) 2003-08-21
WO2003068981A3 true WO2003068981A3 (de) 2003-12-31

Family

ID=27674702

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001590 WO2003068981A2 (de) 2002-02-15 2003-02-17 Verfahren zur reduktion der hintergrund-kontamination nach markierungsreaktionen

Country Status (4)

Country Link
US (1) US20050064481A1 (de)
EP (1) EP1476579A2 (de)
DE (1) DE10206616A1 (de)
WO (1) WO2003068981A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2032701B1 (de) * 2006-06-23 2013-11-27 Alethia Biotherapeutics Inc. An Krebs beteiligte Polynukleotide und Polypeptide
AU2009321508B2 (en) 2008-11-03 2015-03-12 Adc Therapeutics Sa Antibodies that specifically block the biological activity of a tumor antigen
CN103492418B (zh) 2011-03-31 2016-06-29 阿莱斯亚生物疗法股份有限公司 针对肾相关抗原1的抗体及其抗原结合片段
BR112014016723A2 (pt) 2012-01-09 2018-07-24 Alethia Biotherapeutics Inc. método para o tratamento de câncer de mama
US10023902B2 (en) * 2012-09-13 2018-07-17 Purdue Research Foundation Methods for detecting enzyme activity using fluorescence lifetime imaging

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6262252B1 (en) * 1997-05-19 2001-07-17 Mirus, Inc. Single-step method for labeling nucleic acids with mustard or aziridine labeling reagents
US6316610B2 (en) * 1999-02-22 2001-11-13 Pe Corporation (Ny) Labelled oligonucleotides synthesized on solid-supports

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6072043A (en) * 1996-06-04 2000-06-06 Polyprobe, Inc. Optimally fluorescent oligonucleotides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6262252B1 (en) * 1997-05-19 2001-07-17 Mirus, Inc. Single-step method for labeling nucleic acids with mustard or aziridine labeling reagents
US6316610B2 (en) * 1999-02-22 2001-11-13 Pe Corporation (Ny) Labelled oligonucleotides synthesized on solid-supports

Also Published As

Publication number Publication date
WO2003068981A2 (de) 2003-08-21
US20050064481A1 (en) 2005-03-24
DE10206616A1 (de) 2003-09-04
EP1476579A2 (de) 2004-11-17

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