WO2003064681A3 - Assays - Google Patents

Assays Download PDF

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Publication number
WO2003064681A3
WO2003064681A3 PCT/GB2003/000433 GB0300433W WO03064681A3 WO 2003064681 A3 WO2003064681 A3 WO 2003064681A3 GB 0300433 W GB0300433 W GB 0300433W WO 03064681 A3 WO03064681 A3 WO 03064681A3
Authority
WO
WIPO (PCT)
Prior art keywords
secretase
nucleic acid
app
acid molecule
assays
Prior art date
Application number
PCT/GB2003/000433
Other languages
French (fr)
Other versions
WO2003064681A2 (en
Inventor
Fiona Ralitsa Lucas
Joanne Taylor
Original Assignee
Eisai London Res Lab Ltd
Fiona Ralitsa Lucas
Joanne Taylor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai London Res Lab Ltd, Fiona Ralitsa Lucas, Joanne Taylor filed Critical Eisai London Res Lab Ltd
Priority to AU2003238461A priority Critical patent/AU2003238461A1/en
Publication of WO2003064681A2 publication Critical patent/WO2003064681A2/en
Publication of WO2003064681A3 publication Critical patent/WO2003064681A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Abstract

The present invention relates to a protein comprising amyloid precursor protein (APP) and an amino-acid tag, a nucleic acid molecule encoding the protein, a vector comprising the nucleic acid molecule, and a host cell comprising the nucleic acid molecule or the vector. The present invention also relates to assays for measuring Ϝ-secretase activity, and/or simultaneously measuring α-, β-, and Ϝ-secretase activity, which involve utilising the stabilisation of the Ϝ-secretase C-terminal-cleavage product of APP. Such assays are useful in the screening or testing of secretase inhibitors and differentiating such inhibitors from agents that act indirectly, e.g. interface with normal APP biosynthesis and activity.
PCT/GB2003/000433 2002-01-31 2003-01-31 Assays WO2003064681A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003238461A AU2003238461A1 (en) 2002-01-31 2003-01-31 Assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0202276.2 2002-01-31
GB0202276A GB0202276D0 (en) 2002-01-31 2002-01-31 Assays

Publications (2)

Publication Number Publication Date
WO2003064681A2 WO2003064681A2 (en) 2003-08-07
WO2003064681A3 true WO2003064681A3 (en) 2003-12-24

Family

ID=9930151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/000433 WO2003064681A2 (en) 2002-01-31 2003-01-31 Assays

Country Status (3)

Country Link
AU (1) AU2003238461A1 (en)
GB (1) GB0202276D0 (en)
WO (1) WO2003064681A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6518011B1 (en) * 1999-01-13 2003-02-11 Bristol-Myers Squibb Company Method for screening compounds to identify beta-amyloid production modulators

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998015828A1 (en) * 1996-10-07 1998-04-16 Scios Inc. Method to identify direct inhibitors of the beta-amyloid forming enzyme gamma-secretase
WO2000042166A2 (en) * 1999-01-13 2000-07-20 Dupont Pharmaceuticals Company Methods and compositions for monitoring cellular processing of epitope-tagged beta-amyloid precursor protein
US20010034884A1 (en) * 1998-12-07 2001-10-25 Gisela Peraus Abeta-peptide screening assay
WO2001083811A1 (en) * 2000-05-01 2001-11-08 Merck & Co., Inc. Gamma secretase substrates and in vitro assays

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998015828A1 (en) * 1996-10-07 1998-04-16 Scios Inc. Method to identify direct inhibitors of the beta-amyloid forming enzyme gamma-secretase
US20010034884A1 (en) * 1998-12-07 2001-10-25 Gisela Peraus Abeta-peptide screening assay
WO2000042166A2 (en) * 1999-01-13 2000-07-20 Dupont Pharmaceuticals Company Methods and compositions for monitoring cellular processing of epitope-tagged beta-amyloid precursor protein
WO2001083811A1 (en) * 2000-05-01 2001-11-08 Merck & Co., Inc. Gamma secretase substrates and in vitro assays

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BUNNELL WILLIAM L ET AL: "gamma-Secretase cleavage is distinct from endoplasmic reticulum degradation of the transmembrane domain of the amyloid precursor protein", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 48, 27 November 1998 (1998-11-27), pages 31947 - 31955, XP002160358, ISSN: 0021-9258 *
JARVIK J W ET AL: "Epitope tagging.", ANNUAL REVIEW OF GENETICS. UNITED STATES 1998, vol. 32, 1998, pages 601 - 618, XP001015235, ISSN: 0066-4197 *
MACKAY ELAINE A ET AL: "A possible role for cathepsins D, E, and B in the processing of beta-amyloid precursor protein in Alzheimer's disease.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 244, no. 2, 1997, pages 414 - 425, XP001155037, ISSN: 0014-2956 *
SEIFFERT D ET AL: "Positive-negative epitope tagging of beta amyloid precursor protein to identify inhibitors of abeta processing", MOLECULAR BRAIN RESEARCH, ELSEVIER SCIENCE BV, AMSTERDAM, NL, vol. 84, 2000, pages 115 - 126, XP002958539, ISSN: 0169-328X *

Also Published As

Publication number Publication date
AU2003238461A1 (en) 2003-09-02
WO2003064681A2 (en) 2003-08-07
GB0202276D0 (en) 2002-03-20

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