WO2003059926A3 - METHODS AND COMPOSITIONS FOR AMINOACYL-tRNA SYNTHESIS - Google Patents

METHODS AND COMPOSITIONS FOR AMINOACYL-tRNA SYNTHESIS Download PDF

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Publication number
WO2003059926A3
WO2003059926A3 PCT/US2002/041064 US0241064W WO03059926A3 WO 2003059926 A3 WO2003059926 A3 WO 2003059926A3 US 0241064 W US0241064 W US 0241064W WO 03059926 A3 WO03059926 A3 WO 03059926A3
Authority
WO
WIPO (PCT)
Prior art keywords
aminoacyl
trna
group
methods
thf
Prior art date
Application number
PCT/US2002/041064
Other languages
French (fr)
Other versions
WO2003059926A2 (en
Inventor
Biliang Zhang
Zhiyong Cui
Original Assignee
Univ Massachusetts
Biliang Zhang
Zhiyong Cui
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Massachusetts, Biliang Zhang, Zhiyong Cui filed Critical Univ Massachusetts
Priority to AU2002359799A priority Critical patent/AU2002359799A1/en
Publication of WO2003059926A2 publication Critical patent/WO2003059926A2/en
Publication of WO2003059926A3 publication Critical patent/WO2003059926A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods and compositions are disclosed for the high yield chemical synthesis of 2'(3')-O-aminoacylated oligonucleotides, 2'(3')-O-aminoacylated pCpA derivatives and 2'(3')-aminoacyl-tRNA's. The present invention discloses the use of tetrahydrofuranyl group (THF) as a stable protecting group in the production of aminoacyl-oligonucleotides. THF can also be used in conjunction with a removable protecting group, such as dimethoxytrityl group (DMTr). The mild conditions employed for the removal of the THF group are compatible with the integrity of the aminoacyl linkage as well as tRNA, which makes it possible to utilize the methods of the present invention to form aminoacyl-tRNA's. The present invention discloses an efficient route for synthesizing 2'(3')-aminoacyl-pCpA, which can be used to load any natural amino acid, unnatural amino acid, labeled amino acid, reporter group or derivative thereof onto a tRNA molecule through an aminoacyl linkage.
PCT/US2002/041064 2001-12-21 2002-12-20 METHODS AND COMPOSITIONS FOR AMINOACYL-tRNA SYNTHESIS WO2003059926A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002359799A AU2002359799A1 (en) 2001-12-21 2002-12-20 METHODS AND COMPOSITIONS FOR AMINOACYL-tRNA SYNTHESIS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34335301P 2001-12-21 2001-12-21
US60/343,353 2001-12-21

Publications (2)

Publication Number Publication Date
WO2003059926A2 WO2003059926A2 (en) 2003-07-24
WO2003059926A3 true WO2003059926A3 (en) 2003-08-28

Family

ID=23345771

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/041064 WO2003059926A2 (en) 2001-12-21 2002-12-20 METHODS AND COMPOSITIONS FOR AMINOACYL-tRNA SYNTHESIS

Country Status (2)

Country Link
AU (1) AU2002359799A1 (en)
WO (1) WO2003059926A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7288372B2 (en) * 2002-01-17 2007-10-30 Ambergen, Inc. Methods for the preparation of chemically misaminoacylated tRNA via protective groups
CA2498422A1 (en) * 2002-09-09 2004-03-18 Japan Science And Technology Agency Method of aminoacylating trna
WO2004087730A2 (en) * 2003-03-27 2004-10-14 The Johns Hopkins University Method for producing diverse libraries of encoded polymers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NOREN C J ET AL: "A GENERAL METHOD FOR SITE-SPECIFIC INCORPORATION OF UNNATURAL AMINOACIDS INTO PROTEIN", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 244, 14 April 1989 (1989-04-14), pages 182 - 188, XP002925217, ISSN: 0036-8075 *
ROBERTSON S A ET AL: "A GENERAL AND EFFICIENT ROUTE FOR CHEMICAL AMINOACYLATION OF TRANSFER RNAS", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 113, no. 7, 1991, pages 2722 - 2729, XP001064836, ISSN: 0002-7863 *
T. NIINOMI, M. SISIDO: "Synthesis of 2'(3')-O-aminoacyl-pdCpA carrying a photofunctional nonnatural amino acid", CHEMISTRY LETTERS, 1993, pages 1305 - 1308, XP002245631 *

Also Published As

Publication number Publication date
WO2003059926A2 (en) 2003-07-24
AU2002359799A1 (en) 2003-07-30

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