WO2003057848A3 - A method to maintain a genic male-sterile female parental line of wheat through selfing of the maintainer line - Google Patents

A method to maintain a genic male-sterile female parental line of wheat through selfing of the maintainer line Download PDF

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Publication number
WO2003057848A3
WO2003057848A3 PCT/US2002/041852 US0241852W WO03057848A3 WO 2003057848 A3 WO2003057848 A3 WO 2003057848A3 US 0241852 W US0241852 W US 0241852W WO 03057848 A3 WO03057848 A3 WO 03057848A3
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WO
WIPO (PCT)
Prior art keywords
maintainer
line
male
female
wheat
Prior art date
Application number
PCT/US2002/041852
Other languages
French (fr)
Other versions
WO2003057848A2 (en
Inventor
Moshe Feldman
Eitan Millet
Original Assignee
Yeda Res & Dev
Fleit Lois
Moshe Feldman
Eitan Millet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Res & Dev, Fleit Lois, Moshe Feldman, Eitan Millet filed Critical Yeda Res & Dev
Priority to AU2002364613A priority Critical patent/AU2002364613A1/en
Publication of WO2003057848A2 publication Critical patent/WO2003057848A2/en
Publication of WO2003057848A3 publication Critical patent/WO2003057848A3/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022—Genic fertility modification, e.g. apomixis
    • A01H1/023—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention provides a method for stably maintaining a genic male-sterile female parental line of bread wheat, durum wheat and triticale through selfing of the maintainer line. The male-sterile female line is used for the production of low-cost hybrid wheat or hybrid triticale seeds. It also provides a male-sterile female line homozygous for a recessive male-sterility allele, and a maintainer line which is readily and stably propagated. The maintainer line is isogenic to the female line but has an alien chromosomal arm, added to the wheat or triticale complement, carrying a dominant male-fertility allele that restores fertility to the maintainer line, heterologous microgametophytic- and macrogametophytic-suicide genes that kill pollen grains and embryo sacs carrying them thereby preventing the transmission of this chromosome arm to the progeny, a heterologous inducible anti macrogametophytic-suicide gene or anti microgametophytic-suicide gene and a heterologous selectable marker. Because of the activity of the microgametophytic­and macrogametophytic-suicide genes all the progeny of the selfed maintainer are male-sterile female plants. When the activity of the anti macrogametophytic- or anti microgametophytic-suicide gene is induced, also female gametes or male gametes, respectively, carrying the alien chromosome arm are viable and since the frequency of these gametes is about 20%, the progeny of the selfed maintainer contains a mixture of female plants (80%) and maintainer plants (20%). The selectable marker facilitates the selection of maintainer plants among the progeny of the selfed maintainer line and thus, maintenance of the maintainer itself. The female lines and the hybrid lines do not contain heterologous genes and therefore, are not genetically modified organisms (GMO).
PCT/US2002/041852 2001-12-31 2002-12-31 A method to maintain a genic male-sterile female parental line of wheat through selfing of the maintainer line WO2003057848A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002364613A AU2002364613A1 (en) 2001-12-31 2002-12-31 A method to maintain a genic male-sterile female parental line of wheat through selfing of the maintainer line

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34579201P 2001-12-31 2001-12-31
US60/345,792 2001-12-31

Publications (2)

Publication Number Publication Date
WO2003057848A2 WO2003057848A2 (en) 2003-07-17
WO2003057848A3 true WO2003057848A3 (en) 2003-10-30

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Family Applications (1)

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PCT/US2002/041852 WO2003057848A2 (en) 2001-12-31 2002-12-31 A method to maintain a genic male-sterile female parental line of wheat through selfing of the maintainer line

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AU (1) AU2002364613A1 (en)
WO (1) WO2003057848A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7612251B2 (en) 2000-09-26 2009-11-03 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US7517975B2 (en) * 2000-09-26 2009-04-14 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US20070169227A1 (en) 2003-12-16 2007-07-19 Pioneer Hi-Bred International Inc. Dominant Gene Suppression Transgenes and Methods of Using Same
CN104293912A (en) 2003-12-16 2015-01-21 先锋高级育种国际公司 Dominant gene suppression transgenes and methods of using same
US7915478B2 (en) 2007-08-03 2011-03-29 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
US7919676B2 (en) 2007-08-03 2011-04-05 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
US7910802B2 (en) 2007-08-03 2011-03-22 Pioneer Hi-Bred International, Inc. MSCA1 nucleotide sequences impacting plant male fertility and method of using same
CN101779597B (en) * 2010-01-11 2014-06-18 西北农林科技大学 Breeding method for inducing wheat physiological male sterility
AU2012242991B2 (en) 2011-04-11 2017-03-02 Targeted Growth, Inc. Identification and the use of KRP mutants in plants
JP2015532124A (en) 2012-10-23 2015-11-09 モンタナ ステート ユニバーシティ Production of high quality durum wheat with increased amylose content
CN102965391B (en) * 2012-10-23 2014-09-24 中国农业大学 High-efficiency seed labeling method for propagation of plant male sterile line
CN102960234B (en) * 2012-10-23 2014-08-20 中国农业大学 High-efficiency seed labeling method for propagation of plant male sterile line
EP2918164A1 (en) 2014-03-10 2015-09-16 Limagrain Europe Hybrid Cereal Seed Production
EP3675623A1 (en) 2017-08-29 2020-07-08 KWS SAAT SE & Co. KGaA Improved blue aleurone and other segregation systems
CN109156339B (en) * 2018-11-09 2021-04-02 济南市农业科学研究院 Method for improving artificial hybridization efficiency of wheat in Huang-Huai-Hai wheat area by utilizing greenhouse

Citations (6)

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Publication number Priority date Publication date Assignee Title
WO1992001366A1 (en) * 1990-07-17 1992-02-06 Pacific Seeds Pty. Ltd. Production of hybrid cereal crops
US5637785A (en) * 1994-12-21 1997-06-10 The Salk Institute For Biological Studies Genetically modified plants having modulated flower development
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5723763A (en) * 1989-08-10 1998-03-03 Plant Genetic Systems, N.V. Plants with modified flowers
US5773697A (en) * 1996-04-23 1998-06-30 Pioneer Hi-Bred International, Inc. Genetic constructs and methods for producing fruits with very little or diminished seed
WO1998051142A1 (en) * 1997-05-15 1998-11-19 Yeda Research And Development Co. Ltd. Methods for production of hybrid wheat

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723763A (en) * 1989-08-10 1998-03-03 Plant Genetic Systems, N.V. Plants with modified flowers
WO1992001366A1 (en) * 1990-07-17 1992-02-06 Pacific Seeds Pty. Ltd. Production of hybrid cereal crops
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5637785A (en) * 1994-12-21 1997-06-10 The Salk Institute For Biological Studies Genetically modified plants having modulated flower development
US5773697A (en) * 1996-04-23 1998-06-30 Pioneer Hi-Bred International, Inc. Genetic constructs and methods for producing fruits with very little or diminished seed
WO1998051142A1 (en) * 1997-05-15 1998-11-19 Yeda Research And Development Co. Ltd. Methods for production of hybrid wheat

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEE ET AL.: "Isolation of luminidependens: a gene involved in the control of flowering time in arabidopsis", THE PLANT CELL, vol. 6, January 1994 (1994-01-01), pages 75 - 83, XP002019119 *

Also Published As

Publication number Publication date
AU2002364613A8 (en) 2003-07-24
WO2003057848A2 (en) 2003-07-17
AU2002364613A1 (en) 2003-07-24

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