WO2003056029A3 - Highly sensitive and continuous protein-tyrosine-phosphatase (ptpase) test using 6,8 difluoro-4-methyl-umbelliferylphosphate - Google Patents

Highly sensitive and continuous protein-tyrosine-phosphatase (ptpase) test using 6,8 difluoro-4-methyl-umbelliferylphosphate Download PDF

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Publication number
WO2003056029A3
WO2003056029A3 PCT/EP2002/014755 EP0214755W WO03056029A3 WO 2003056029 A3 WO2003056029 A3 WO 2003056029A3 EP 0214755 W EP0214755 W EP 0214755W WO 03056029 A3 WO03056029 A3 WO 03056029A3
Authority
WO
WIPO (PCT)
Prior art keywords
difluoro
phosphatase
tyrosine
umbelliferylphosphate
ptpase
Prior art date
Application number
PCT/EP2002/014755
Other languages
German (de)
French (fr)
Other versions
WO2003056029A2 (en
Inventor
Stefan Welte
Norbert Tennagels
Stefan Petry
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2002100173 external-priority patent/DE10200173A1/en
Priority claimed from DE2002136329 external-priority patent/DE10236329A1/en
Priority to EP02796734A priority Critical patent/EP1466009A2/en
Priority to KR10-2004-7010451A priority patent/KR20040073539A/en
Priority to AU2002361217A priority patent/AU2002361217A1/en
Priority to IL16283202A priority patent/IL162832A0/en
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Priority to JP2003556546A priority patent/JP2005512600A/en
Priority to MXPA04006361A priority patent/MXPA04006361A/en
Priority to CA002471601A priority patent/CA2471601A1/en
Publication of WO2003056029A2 publication Critical patent/WO2003056029A2/en
Publication of WO2003056029A3 publication Critical patent/WO2003056029A3/en
Priority to NO20043244A priority patent/NO20043244L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Abstract

The invention relates to a method for detecting a protein-tyrosine-phosphatase in biological material by using 6,8-difluoro-4-methylumbelliferylphosphate (DiFMUP).
PCT/EP2002/014755 2002-01-04 2002-12-24 Highly sensitive and continuous protein-tyrosine-phosphatase (ptpase) test using 6,8 difluoro-4-methyl-umbelliferylphosphate WO2003056029A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CA002471601A CA2471601A1 (en) 2002-01-04 2002-12-24 Highly sensitive and continuous protein-tyrosine-phosphatase (ptpase) test using 6,8 difluoro-4-methyl-umbelliferylphosphate
MXPA04006361A MXPA04006361A (en) 2002-01-04 2002-12-24 Highly sensitive and continuous protein-tyrosine-phosphatase (ptpase) test using 6.8 difluoro-4-methyl-umbelliferylphosphate.
KR10-2004-7010451A KR20040073539A (en) 2002-01-04 2002-12-24 Highly sensitive and continuous protein-tyrosine-phosphatase(PTPase) test using 6,8-difluoro-4-methyl-umbelliferylphosphate
AU2002361217A AU2002361217A1 (en) 2002-01-04 2002-12-24 Highly sensitive and continuous protein-tyrosine-phosphatase (PTPase) test using 6,8 difluoro-4-methyl-umbelliferylphosphate
IL16283202A IL162832A0 (en) 2002-01-04 2002-12-24 Highly sensitive and continous protein-tyrosine-phosphatase (ptpase) test using 6,8 difluoro-4-methylumbe
EP02796734A EP1466009A2 (en) 2002-01-04 2002-12-24 Highly sensitive and continuous protein-tyrosine-phosphatase (ptpase) test using 6.8 difluoro-4-methyl-umbelliferylphosphate
JP2003556546A JP2005512600A (en) 2002-01-04 2002-12-24 Highly sensitive continuous protein tyrosine phosphatase (PTPase) assay using 6,8-difluoro-4-methylumbelliferyl phosphate
NO20043244A NO20043244L (en) 2002-01-04 2004-08-02 Highly sensitive and continuous protein tyrosine phosphatase (PTPASE) test using 6,8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE2002100173 DE10200173A1 (en) 2002-01-04 2002-01-04 Detecting activity of protein-tyrosine phosphatase by detecting hydrolysis of 6,8-difluoro-4-methylumbelliferyl phosphate, useful for identifying modulators of the enzyme that can be used to treat diabetes
DE10200173.1 2002-01-04
DE10236329.3 2002-08-08
DE2002136329 DE10236329A1 (en) 2002-08-08 2002-08-08 Detecting activity of protein-tyrosine phosphatase by detecting hydrolysis of 6,8-difluoro-4-methylumbelliferyl phosphate, useful for identifying modulators of the enzyme that can be used to treat diabetes

Publications (2)

Publication Number Publication Date
WO2003056029A2 WO2003056029A2 (en) 2003-07-10
WO2003056029A3 true WO2003056029A3 (en) 2004-04-01

Family

ID=26010898

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/014755 WO2003056029A2 (en) 2002-01-04 2002-12-24 Highly sensitive and continuous protein-tyrosine-phosphatase (ptpase) test using 6,8 difluoro-4-methyl-umbelliferylphosphate

Country Status (12)

Country Link
EP (1) EP1466009A2 (en)
JP (1) JP2005512600A (en)
KR (1) KR20040073539A (en)
CN (1) CN1612939A (en)
AU (1) AU2002361217A1 (en)
BR (1) BR0215452A (en)
CA (1) CA2471601A1 (en)
IL (1) IL162832A0 (en)
MX (1) MXPA04006361A (en)
NO (1) NO20043244L (en)
RU (1) RU2004123794A (en)
WO (1) WO2003056029A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100350045C (en) * 2003-08-21 2007-11-21 北京农业生物技术研究中心 Corn tyrosin protein phosphatase gene and its coding protein and use
US8008035B2 (en) 2007-01-25 2011-08-30 Roche Diagnostics Operations, Inc. Enhancement of vanadium-containing phosphatase inhibitors
EP1949894B1 (en) * 2007-01-25 2016-06-29 Roche Diagnostics GmbH Enhancement of vanadium-containing phosphatase inhibitors by polyols
CN113155787A (en) * 2020-12-29 2021-07-23 武汉合研生物医药科技有限公司 Activity detection method of mutant enzyme SHP 2E 76K

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999061410A1 (en) * 1998-05-12 1999-12-02 American Home Products Corporation 2,3,5-substituted biphenyls useful in the treatment of insulin resistance and hyperglycemia

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999061410A1 (en) * 1998-05-12 1999-12-02 American Home Products Corporation 2,3,5-substituted biphenyls useful in the treatment of insulin resistance and hyperglycemia

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GOLDSTEIN B J: "Protein-tyrosine phosphatase 1B (PTP1B): a novel therapeutic target for type 2 diabetes mellitus, obesity and related states of insulin resistance.", CURRENT DRUG TARGETS. IMMUNE, ENDOCRINE AND METABOLIC DISORDERS. NETHERLANDS NOV 2001, vol. 1, no. 3, November 2001 (2001-11-01), pages 265 - 275, XP001153562, ISSN: 1568-0088 *
KERBY M ET AL: "A fluorogenic assay using pressure-driven flow on a microchip.", ELECTROPHORESIS. GERMANY OCT 2001, vol. 22, no. 18, October 2001 (2001-10-01), pages 3916 - 3923, XP001153559, ISSN: 0173-0835 *
MALAMAS M S ET AL: "Novel Benzofuran and Benzothiophene Biphenyls as Inhibitors of Protein Tyrosine Phophatase 1B with Antihyperglycemic Properties", JOURNAL OF MEDICINAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. WASHINGTON, US, vol. 43, no. 7, 6 April 2000 (2000-04-06), pages 1293 - 1310, XP002190818, ISSN: 0022-2623 *
MATTER WILLIAM F ET AL: "Role of PRL-3, a human muscle-specific tyrosine phosphatase, in angiotensin-II signaling.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 283, no. 5, 25 May 2001 (2001-05-25), pages 1061 - 1068, XP002247489, ISSN: 0006-291X *
WROBEL JAY ET AL: "PTP1B inhibition and antihyperglycemic activity in the ob/ob mouse model of novel 11-arylbenzo(b)naphtho(2,3-d)furans and 11-arylbenzo(b)naphtho(2,3-d)thiophenes", JOURNAL OF MEDICINAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. WASHINGTON, US, vol. 42, no. 17, 26 August 1999 (1999-08-26), pages 3199 - 3202, XP002181876, ISSN: 0022-2623 *

Also Published As

Publication number Publication date
MXPA04006361A (en) 2004-10-04
EP1466009A2 (en) 2004-10-13
CA2471601A1 (en) 2003-07-10
AU2002361217A1 (en) 2003-07-15
RU2004123794A (en) 2005-04-20
IL162832A0 (en) 2005-11-20
KR20040073539A (en) 2004-08-19
JP2005512600A (en) 2005-05-12
WO2003056029A2 (en) 2003-07-10
NO20043244L (en) 2004-08-02
CN1612939A (en) 2005-05-04
BR0215452A (en) 2004-11-23

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