WO2003054168A3 - Defined competitive exclusion cultures for food borne pathogens - Google Patents

Defined competitive exclusion cultures for food borne pathogens Download PDF

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Publication number
WO2003054168A3
WO2003054168A3 PCT/US2002/041459 US0241459W WO03054168A3 WO 2003054168 A3 WO2003054168 A3 WO 2003054168A3 US 0241459 W US0241459 W US 0241459W WO 03054168 A3 WO03054168 A3 WO 03054168A3
Authority
WO
WIPO (PCT)
Prior art keywords
species
isolates
salmonella
pathogens
microflora
Prior art date
Application number
PCT/US2002/041459
Other languages
French (fr)
Other versions
WO2003054168A2 (en
Inventor
Billy M Hargis
Daniel J Donoghue
Ann M Donoghue
Noel K Neighbor
Lisa R Bielke
Lisa A Newberry
Original Assignee
Univ Arkansas
Us Agriculture
Billy M Hargis
Daniel J Donoghue
Ann M Donoghue
Noel K Neighbor
Lisa R Bielke
Lisa A Newberry
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Arkansas, Us Agriculture, Billy M Hargis, Daniel J Donoghue, Ann M Donoghue, Noel K Neighbor, Lisa R Bielke, Lisa A Newberry filed Critical Univ Arkansas
Priority to AU2002359858A priority Critical patent/AU2002359858A1/en
Priority to US10/493,091 priority patent/US20040241150A1/en
Publication of WO2003054168A2 publication Critical patent/WO2003054168A2/en
Publication of WO2003054168A3 publication Critical patent/WO2003054168A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

The invention comprises a method of screening cecal microflora for in vitro competition against select enteric pathogens, as well as the characterization of specific cecal microflora. The invention also comprises using the characterized competitive exclusion culture against select enteric pathogens in poultry, porcine, and ruminant, and humans. Selection of competitive isolates was achieved by combining Salmonella enteritidis (SE), adult broiler cecal microflora, and an indicator broth, which was then placed in a microtiter plate. Potential isolates were then challenged individually against Salmonella enteritidis. The ability of the selective isolates was determined by qualitative evaluation of Salmonella growth on brilliant green agar after the challenge. Isolates analyzed included but are not limited to; Bacillus species, Staphylococcus species, Escherichia species, Citrobacter species, Enterococcus species, Proteus species, Enterobacter species, Salmonella species, and Klebsiella species. Each isolate from genera with potential pathogens was further safety-tested by intraperitoneal injection or airsac injection and evaluated for morbidity, mortality, and lesions. We demonstrate that the incidence of SE recovery was markedly reduced by treatment with nine selected isolates which were individually amplified, under aerobic conditions, and then re-combined prior to administration.
PCT/US2002/041459 2001-12-20 2002-12-19 Defined competitive exclusion cultures for food borne pathogens WO2003054168A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002359858A AU2002359858A1 (en) 2001-12-20 2002-12-19 Defined competitive exclusion cultures for food borne pathogens
US10/493,091 US20040241150A1 (en) 2002-12-19 2002-12-19 Defined competitive exclusion cultures for food borne pathogens

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34268801P 2001-12-20 2001-12-20
US60/342,688 2001-12-20

Publications (2)

Publication Number Publication Date
WO2003054168A2 WO2003054168A2 (en) 2003-07-03
WO2003054168A3 true WO2003054168A3 (en) 2003-09-12

Family

ID=23342856

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/041459 WO2003054168A2 (en) 2001-12-20 2002-12-19 Defined competitive exclusion cultures for food borne pathogens

Country Status (2)

Country Link
AU (1) AU2002359858A1 (en)
WO (1) WO2003054168A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT501919B1 (en) 2005-06-14 2008-09-15 Erber Ag PROBIOTIC, HEALTH OR BZW. PERFORMANCE-ADDING FEED AND / OR DRINKING WATER SUPPLEMENT FOR ANIMALS AND ITS USE
CN107022505A (en) * 2017-03-30 2017-08-08 东北师范大学 A kind of citric acid bacillus and its application for heavy metal copper ion remaval
CN110777103B (en) * 2019-12-07 2021-06-01 浙江爱科乐环保有限公司 Simple and easy and long-distance transportation rapid production method of biological deodorization bactericide

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466445A (en) * 1985-06-18 1995-11-14 Emory University Methods for reducing salmonella in chickens
US5824322A (en) * 1995-08-21 1998-10-20 Cytrx Corporation Compositions and methods for growth promotion
US20030031659A1 (en) * 1998-08-24 2003-02-13 Sean Farmer Probiotic, lactic acid-producing bacteria and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466445A (en) * 1985-06-18 1995-11-14 Emory University Methods for reducing salmonella in chickens
US5824322A (en) * 1995-08-21 1998-10-20 Cytrx Corporation Compositions and methods for growth promotion
US20030031659A1 (en) * 1998-08-24 2003-02-13 Sean Farmer Probiotic, lactic acid-producing bacteria and uses thereof

Also Published As

Publication number Publication date
AU2002359858A1 (en) 2003-07-09
WO2003054168A2 (en) 2003-07-03
AU2002359858A8 (en) 2003-07-09

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