WO2003045141A1 - Echantillon de dent blanchi et son procede de production - Google Patents

Echantillon de dent blanchi et son procede de production Download PDF

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Publication number
WO2003045141A1
WO2003045141A1 PCT/JP2001/010360 JP0110360W WO03045141A1 WO 2003045141 A1 WO2003045141 A1 WO 2003045141A1 JP 0110360 W JP0110360 W JP 0110360W WO 03045141 A1 WO03045141 A1 WO 03045141A1
Authority
WO
WIPO (PCT)
Prior art keywords
tooth
energy
teeth
pulp
curable composition
Prior art date
Application number
PCT/JP2001/010360
Other languages
English (en)
Japanese (ja)
Inventor
Tsuneyuki Yamamoto
Original Assignee
Hokkaido Technology Licensing Office Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hokkaido Technology Licensing Office Co., Ltd. filed Critical Hokkaido Technology Licensing Office Co., Ltd.
Priority to AU2002222554A priority Critical patent/AU2002222554A1/en
Priority to PCT/JP2001/010360 priority patent/WO2003045141A1/fr
Priority to JP2003546657A priority patent/JPWO2003045141A1/ja
Publication of WO2003045141A1 publication Critical patent/WO2003045141A1/fr

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Classifications

    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
    • G09B23/283—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine for dentistry or oral hygiene

Definitions

  • the present invention relates to a transparent sample of teeth made from animal (eg, human) teeth and a method for producing the same.
  • the method of manufacturing this transparent specimen includes (1) a step of perforating the pulp chamber and dissolving the pulp tissue with hydrogen peroxide water, (2) a step of injecting ink into the root canal, and (3) a high humidity with the crown facing upward. (4) denitrating the teeth using nitric acid, and (5) immersing in phenol to make it transparent. Thereafter, the improved method provided by Ono included (1) a step of perforating the pulp chamber and dissolving the pulp tissue with dental antiformin, (2) a step of immersing the teeth in acetone, and (3) a step of immersing the teeth in 60. (4) denitrating the teeth using nitric acid, and (5) phenol or salicylic acid. It consists of a step of immersing in methyl to make it transparent.
  • the transparent tooth specimen infused with black ink produced by these methods is excellent in that it can observe the root canal that has been dyed black through the dentin without destroying the root, and is most suitable for studying the root canal morphology. It has been used frequently.
  • the present invention relates to a method for producing a transparent specimen of an animal tooth
  • FIG. 1 is a schematic diagram of a photograph showing a mold of a crown before decalcification taken with a silicone impression material.
  • Fig. 2 is a schematic view of a photograph showing the infusion of black ink using a syringe into the pulp chamber (right) and the root canal (left) after decalcification and division.
  • Fig. 3 shows that after dehydration, the specimen clarified with methyl salicylate was immersed in a mixture of styrene monomer, styrene monomer and rigorac in a ratio of 1: 1, 1: 2, and 1: 3, and finally immersed in pure rigorac.
  • Fig. 4 is a schematic diagram of a photograph showing that a rigorac is infiltrated into a specimen.
  • FIG. 4 is a schematic view of a photograph showing that excess rigorac is being wiped from the specimen surface immediately before polymerization.
  • FIG. 5 is a schematic view of a photograph showing the re-joining of the pulp chamber (crown) and the root canal.
  • FIG. 6 is a schematic view of a photograph showing the re-joined specimen being put into an ultraviolet polymerization vessel.
  • FIG. 7 is a schematic view of a photograph showing a resin mold containing a hardening agent being poured into a mold for a crown already taken.
  • FIG. 8 is a schematic view of a photograph showing a state where a transparent sample is inserted and the urethane resin is waiting for curing.
  • FIG. 9 is a schematic view of a photograph showing a state where a transparent sample was taken out after the urethane resin was cured.
  • FIG. 10 is a schematic view of a photograph showing a crown and a transparent specimen reconstructed with urethane resin.
  • FIG. 11 is a schematic view of a photograph showing transparent specimens of various tooth types in which the crown has been reconstructed.
  • Animal refers to any animal that has teeth. Examples include mammals such as humans, dogs and cats. This kind of transparent specimen is used for humans, for example, in lectures at dentistry courses at universities, etc., and for explanations to patients at dental clinics. For animals other than humans, for example, veterinary medicine at universities, etc. It is used for lectures and explanations to owners at animal hospitals.
  • Transparent means that the colored pulp chamber and root canal are observable to the naked eye from the outside, and includes not only colorless and transparent but also colored transparent.
  • Coding is not particularly limited as long as the pulp chamber and the root canal can be distinguished when the transparent specimen is viewed from the outside with the naked eye. Preferably, black is used.
  • the “transparent agent” is not particularly limited as long as it is a substance that makes the tooth from which enamel has been removed transparent.
  • methyl salicylate and phenol are exemplified, and methyl salicylate is preferred.
  • the “energy-curable composition” is not particularly limited as long as it is a composition that is cured by applying energy such as heat or light and does not lose the transparency of teeth after curing.
  • Rigolac TM manufactured by Showa Polymer Co., Ltd.
  • whose curing component is an ultraviolet-curable unsaturated polyester resin, may be mentioned.
  • step (a) as a method of taking a mold of a tooth crown, for example, a method using a dental impression material can be mentioned. Before taking the mold, it is preferable to brush the tooth surface using a dental scaler and a strong alkaline aqueous solution such as K ⁇ H or NaOH.
  • step (b) as a method for removing the minerals of the teeth, there is a method using an acid chelating agent.
  • a method of immersing a tooth in a formic acid solution for several weeks can be mentioned.
  • a method of removing pulp from the teeth in the step (c) there is a method of cutting the teeth, dividing the pulp into a pulp chamber and a root canal, and removing the pulp from each.
  • the method for producing a transparent specimen of an animal tooth comprising: (a) removing the crown of the tooth; (b) removing minerals from the tooth; (c) removing pulp from the tooth. Removing and coloring the pulp chamber and the root canal; (d) translucent the tooth from which minerals have been removed with a translucent agent; (e) applying an energy-curable composition to the transparentized tooth. (F) applying the predetermined energy to cure the energy-curable composition; and (g) using the mold of the crown to remove the enamel from the tooth. Further comprising the step of removably forming a layer having the same shape as the enamel of the original tooth.
  • a method of coloring the pulp cavity and the root canal in the step (c) for example, a method of depositing a coloring agent on the exposed pulp chamber wall and the root canal wall after removing the pulp can be mentioned.
  • the coloring agent is not particularly limited as long as it is easily deposited on these walls and hardly eluted in the subsequent processing, and examples thereof include black ink.
  • a method of penetrating and attaching a coloring agent to the pulp chamber and the root canal by the suction means from the apical hole at the root of the tooth is mentioned. According to this method, a tooth cutting step is not required, which is preferable in manufacturing.
  • step (d) the teeth from which the inorganic material has been removed are immersed in a transparent agent.
  • the immersion time depends on the size of the teeth and the type of clarifying agent used. For example, when methyl salicylate is used as the clarifying agent, any tooth can be clarified in 24 to 48 hours. It is preferred that the teeth be dehydrated with a dehydrating agent such as ethanol prior to this step.
  • a plurality of immersion liquids in which the concentration of the energy-curable composition is changed with an appropriate solvent are used, and the steps are performed in the immersion liquid having a low concentration of the energy-curable composition and a high concentration. It is preferred to immerse the transparentized teeth. By gradually increasing the concentration in this way, the transparent agent that has permeated into the teeth can be reliably replaced with the energy-curing composition. Note that only the solvent may be used as the first immersion liquid, and only the energy-curable composition may be used as the last immersion liquid.
  • the solvent is not particularly limited as long as it is a substance that is compatible with the clarifying agent and the energy-curable composition.
  • a styrene monomer may be used. It is suitable.
  • step (f) energy corresponding to the energy-curable composition to be used is applied to cure the composition.
  • energy corresponding to the energy-curable composition to be used is applied to cure the composition.
  • a thermosetting composition when using a thermosetting composition, a photocurable composition is used, light of an appropriate wavelength is irradiated.
  • step (g) for example, first, an appropriate anti-adhesion sheet (for example, manufactured by Parafilm Sanko Junyaku Co., Ltd.) is coated on the sample cured in the step (f), and then the appropriate curing is performed.
  • the acidic composition is poured into the mold obtained in step (a), and after at least the crown portion of the sample is inserted, the composition is cured. Then, after hardening, it is pulled out from the specimen, the shape is adjusted, and an enamel layer model is created.
  • the curable composition used here is: 1) cured even when the air force is shut off, 2) the cured model has elasticity for easy attachment and detachment, and 3) the cured product obtained in step (f) It is preferable that it has the property of not adhering to the substrate.
  • a white urethane resin having these properties and having the highest durability is particularly suitable.
  • the transparent specimen thus obtained can be handled freely with bare hands by embedding it in the resin, making observation and photography much easier than with conventional ones.
  • the coloring agent black ink
  • the original pulp morphology can be observed.
  • the specimen can be given a natural shape as a tooth. Also, by removing the enamel layer, the morphology of the coronal dentin and the medulla can be observed.
  • a set of 28 teeth, excluding the third molar, collected from humans was prepared, and these were combined with a dental scaler at 30% (5.35 N) K ⁇ . H tooth solution was used to brush the tooth surface.
  • a dental impression mold for dental use (trade name: JM Silicone, manufacturer: Nissin Co., Ltd.) was used to mold the crown of these teeth. These teeth were immersed in 5% formic acid for 2 weeks to completely remove the minerals from the teeth.
  • Fig. 1 a dental impression mold for dental use
  • the tooth was cut with a force razor to divide it into a crown and a root, and the pulp was extracted from the exposed pulp chamber and root canal. And removed it. Then, black ink was injected with a syringe into the pulp chamber from which the pulp was extracted and the root canal. Next, after removing excess ink, the teeth were immersed in 70%, 80%, 90%, and 100% ethanol, respectively, for 24 hours to dehydrate. Then, as shown in FIG. 3, the teeth after dehydration were immersed in methyl salicylate for 48 hours. As a result, a tooth was obtained in which the pulp chamber and the root canal, which were colored black with black ink, were transparent to the extent that they could be visually observed from the outside. Next, as shown in FIG.
  • the clarified teeth were placed in a styrene monomer for 24 hours and in a mixture of styrene monomer and Rigolac® ⁇ 1: 1 (volume ratio) ⁇ for 24 hours in styrene.
  • curing agent product name
  • QCU-3 Manufacturer Nisshin EM Co., Ltd.
  • Parafilm® is wrapped very thinly around the crown portion of the reassembled tooth, and, as shown in FIG. 7, on the other hand, as shown in FIG.
  • the base material Takenate, curing agent: Evening Kerac (Manufacturer: Takeda Mitsui Chemicals, Inc.) was poured, and the crown was inserted into the urethane resin as shown in FIG. Then, it was left for 48 hours in this state to be cured.
  • the transparent sample and the cured urethane resin were separated from the mold, and the excess urethane resin was cut along the cervical line. After that, unnecessary Parafilm® was peeled off from the sample.
  • the shaped urethane resin was placed in a high-temperature bath at 60 days and nights to be completely cured.
  • the specimens produced in this way are shown in FIGS. 10 and 11.
  • the enamel layer made of urethane resin was removable, and the specimen had a light amber color, so that the black color of the pulp chamber and the root canal wall could be clearly confirmed.
  • Industrial applicability As described above, the transparent specimen of the tooth according to the present invention can be handled freely with bare hands, which is useful for observation and photography, and since the pulp chamber is not destroyed, it is suitable for observation of the original pulp morphology. It is useful, and moreover, because the enamel layer is detachable, it is also useful for observing the morphology of the coronary dentin and the medulla.

Abstract

L'invention concerne un procédé permettant de produire un échantillon blanchi d'une dent d'animal. Ledit procédé est caractérisé en qu'il consiste à (a) prendre une impression de la couronne d'une dent, à (b) éliminer les matières inorganiques de la dent, à (c) éliminer la pulpe de la dent et à colorer la chambre pulpaire et le canal pulpaire, à (d) blanchir la dent, de laquelle on a enlevé les matières inorganiques au moyen d'un agent de blanchiment, (e) à faire pénétrer dans la dent nettoyée une composition durcissable par application d'énergie, (f) à durcir ladite composition par application d'énergie, et (g) à former une couche détachable dotée de la même forme que l'émail de la dent d'origine sur la dent susmentionnée, dont l'émail a été enlevé, à l'aide du modèle de couronne susmentionné.
PCT/JP2001/010360 2001-11-28 2001-11-28 Echantillon de dent blanchi et son procede de production WO2003045141A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002222554A AU2002222554A1 (en) 2001-11-28 2001-11-28 Clear tooth sample and process for producing the same
PCT/JP2001/010360 WO2003045141A1 (fr) 2001-11-28 2001-11-28 Echantillon de dent blanchi et son procede de production
JP2003546657A JPWO2003045141A1 (ja) 2001-11-28 2001-11-28 歯の透明標本及びその製造方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2001/010360 WO2003045141A1 (fr) 2001-11-28 2001-11-28 Echantillon de dent blanchi et son procede de production

Publications (1)

Publication Number Publication Date
WO2003045141A1 true WO2003045141A1 (fr) 2003-06-05

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AU (1) AU2002222554A1 (fr)
WO (1) WO2003045141A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101299395B1 (ko) 2012-12-12 2013-08-22 김상헌 무기물 및 유기물의 구성비가 조절된 치아 골 이식재 및 그 제조방법
WO2014069519A1 (fr) * 2012-10-30 2014-05-08 学校法人金沢医科大学 Trousse pour la production d'un échantillon biologique rendu transparent, et procédé de production d'un échantillon biologique rendu transparent

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TSUNEYUKI YAMAMOTO: "Shiga toumei hyouhon no resin houmai ni tsuite", NIIGATA SHIGAKKAI ZASSHI, NIIGATA SHIGAKKAI, vol. 31, no. 1, 20 July 2001 (2001-07-20), pages 51, XP002909012 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014069519A1 (fr) * 2012-10-30 2014-05-08 学校法人金沢医科大学 Trousse pour la production d'un échantillon biologique rendu transparent, et procédé de production d'un échantillon biologique rendu transparent
US11035763B2 (en) 2012-10-30 2021-06-15 Kanazawa Medical University Kit for producing cleared biological specimens and method for producing cleared biological specimens
KR101299395B1 (ko) 2012-12-12 2013-08-22 김상헌 무기물 및 유기물의 구성비가 조절된 치아 골 이식재 및 그 제조방법

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AU2002222554A1 (en) 2003-06-10
JPWO2003045141A1 (ja) 2005-04-07

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