WO2003035906A3 - Method for genotyping microsatellite dna markers by mass spectrometry - Google Patents

Method for genotyping microsatellite dna markers by mass spectrometry Download PDF

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Publication number
WO2003035906A3
WO2003035906A3 PCT/IB2002/004157 IB0204157W WO03035906A3 WO 2003035906 A3 WO2003035906 A3 WO 2003035906A3 IB 0204157 W IB0204157 W IB 0204157W WO 03035906 A3 WO03035906 A3 WO 03035906A3
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WO
WIPO (PCT)
Prior art keywords
mass spectrometry
dna markers
microsatellite dna
genotyping
genotyping microsatellite
Prior art date
Application number
PCT/IB2002/004157
Other languages
French (fr)
Other versions
WO2003035906A2 (en
Inventor
Abdelmajid Belouchi
Diane Saint-Louis
Bruno Paquin
Original Assignee
Galileo Genomics
Abdelmajid Belouchi
Diane Saint-Louis
Bruno Paquin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Galileo Genomics, Abdelmajid Belouchi, Diane Saint-Louis, Bruno Paquin filed Critical Galileo Genomics
Priority to US10/493,401 priority Critical patent/US20050064419A1/en
Publication of WO2003035906A2 publication Critical patent/WO2003035906A2/en
Publication of WO2003035906A3 publication Critical patent/WO2003035906A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry

Abstract

The present invention relates to a method for genotyping microsatellite DNA markers. The described protocol is to convert a double-stranded PCR fragment encoding the microsatellite of interest into a single-stranded DNA fragment of approximately 30-50 nucleotides. The produced fragment comprises the repeated region with a few flanking nucleotides and is suitable for analysis by mass spectrometry .
PCT/IB2002/004157 2001-10-26 2002-10-07 Method for genotyping microsatellite dna markers by mass spectrometry WO2003035906A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US10/493,401 US20050064419A1 (en) 2001-10-26 2002-10-07 Method for genotyping microsatellite dna markers by mass spectrometry

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33506801P 2001-10-26 2001-10-26
US60/335,068 2001-10-26

Publications (2)

Publication Number Publication Date
WO2003035906A2 WO2003035906A2 (en) 2003-05-01
WO2003035906A3 true WO2003035906A3 (en) 2003-11-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2002/004157 WO2003035906A2 (en) 2001-10-26 2002-10-07 Method for genotyping microsatellite dna markers by mass spectrometry

Country Status (1)

Country Link
WO (1) WO2003035906A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0624643A2 (en) * 1993-05-11 1994-11-17 Becton, Dickinson and Company Decontamination of nucleic acid amplification reactions
US5869242A (en) * 1995-09-18 1999-02-09 Myriad Genetics, Inc. Mass spectrometry to assess DNA sequence polymorphisms
WO1999014375A2 (en) * 1997-09-19 1999-03-25 Genetrace Systems, Inc. Dna typing by mass spectrometry with polymorphic dna repeat markers
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0624643A2 (en) * 1993-05-11 1994-11-17 Becton, Dickinson and Company Decontamination of nucleic acid amplification reactions
US5869242A (en) * 1995-09-18 1999-02-09 Myriad Genetics, Inc. Mass spectrometry to assess DNA sequence polymorphisms
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
WO1999014375A2 (en) * 1997-09-19 1999-03-25 Genetrace Systems, Inc. Dna typing by mass spectrometry with polymorphic dna repeat markers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANDREAS BRAUN ET AL: "Improved Analysis of Microsatellites Using Mass spectrometry", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 46, 1997, pages 18 - 23, XP002162453, ISSN: 0888-7543 *
MAXAM A M ET AL: "A NEW METHOD FOR SEQUENCING DNA", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, NEW YORK, NY, US, vol. 74, no. 2, 1 February 1977 (1977-02-01), pages 560 - 564, XP000604550 *
WADA Y ET AL: "MEASUREMENT OF POLYMORPHIC TRINUCLEOTIDE REPEATS IN THE ANDROGEN RECEPTOR GENE BY TIME-OF-FLIGHT MASS SPECTROMETRY", JOURNAL OF MASS SPECTROMETRY, WILEY, CHICHESTER, GB, vol. 34, no. 8, August 1999 (1999-08-01), pages 885 - 888, XP008013336, ISSN: 1076-5174 *

Also Published As

Publication number Publication date
WO2003035906A2 (en) 2003-05-01

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