WO2003035905A3 - Methode de determination de l'activite bacteriostatique ou bactericide des agents antibiotiques par pcr quantitative - Google Patents

Methode de determination de l'activite bacteriostatique ou bactericide des agents antibiotiques par pcr quantitative Download PDF

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Publication number
WO2003035905A3
WO2003035905A3 PCT/FR2002/003566 FR0203566W WO03035905A3 WO 2003035905 A3 WO2003035905 A3 WO 2003035905A3 FR 0203566 W FR0203566 W FR 0203566W WO 03035905 A3 WO03035905 A3 WO 03035905A3
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WO
WIPO (PCT)
Prior art keywords
antibiotic
quantitative pcr
bactericidal activity
bacterial count
antibiotic agents
Prior art date
Application number
PCT/FR2002/003566
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English (en)
Other versions
WO2003035905A2 (fr
Inventor
Didier Raoult
Max Maurin
Jean-Marc Rolain
Original Assignee
Univ Aix Marseille Ii
Didier Raoult
Max Maurin
Jean-Marc Rolain
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Aix Marseille Ii, Didier Raoult, Max Maurin, Jean-Marc Rolain filed Critical Univ Aix Marseille Ii
Publication of WO2003035905A2 publication Critical patent/WO2003035905A2/fr
Publication of WO2003035905A3 publication Critical patent/WO2003035905A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode de détermination de la sensibilité des bactéries (effet bactériostatique, soit concentration minimale inhibitrice ou CMI) en milieu cellulaire ou en milieu axénique, par titrage d'une suspension bactérienne en présence ou non d'un antibiotique testé à une ou plusieurs concentrations données, par méthode de PCR quantitative. Dans ces conditions, un effet bactériostatique correspond à un titre bactérien après incubation en présence de l'antibiotique inférieur ou égal à celui du titre bactérien initial (ou inoculum), alors qu'un témoin sans antibiotique (témoin de croissance) montre une élévation progressive du titre bactérien au cours du temps.
PCT/FR2002/003566 2001-10-22 2002-10-18 Methode de determination de l'activite bacteriostatique ou bactericide des agents antibiotiques par pcr quantitative WO2003035905A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0113573A FR2831187A1 (fr) 2001-10-22 2001-10-22 Methode de determination de l'activite bacteriostatique ou bactericide des agents antibiotiques par pcr quantitative
FR01/13573 2001-10-22

Publications (2)

Publication Number Publication Date
WO2003035905A2 WO2003035905A2 (fr) 2003-05-01
WO2003035905A3 true WO2003035905A3 (fr) 2004-02-12

Family

ID=8868536

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/003566 WO2003035905A2 (fr) 2001-10-22 2002-10-18 Methode de determination de l'activite bacteriostatique ou bactericide des agents antibiotiques par pcr quantitative

Country Status (2)

Country Link
FR (1) FR2831187A1 (fr)
WO (1) WO2003035905A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0324650D0 (en) 2003-10-22 2003-11-26 Acolyte Biomedica Ltd Using nucleic acids for clinical microbiology testing
GB201716883D0 (en) * 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
CN112176024B (zh) * 2020-08-07 2023-07-14 深圳艾尔生物科技有限公司 一种单细胞计数检测抗生素对细菌抑制的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0786519A2 (fr) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Polynucléotides et séquences de Staphylococcus aureus
US5786147A (en) * 1995-09-18 1998-07-28 Bio Merieux Detection of enterobacteria
US5976791A (en) * 1994-08-24 1999-11-02 Bio Merieux Nucleotide fragments capable of hybridizing specifically to rickettsia rDNA or rRNA and their use as probes or primers
FR2798386A1 (fr) * 1999-09-10 2001-03-16 Didier Raoult Oligonucleotides monocatenaires, sondes, amorces et procede de detection des spirochetes
US6280946B2 (en) * 1998-08-07 2001-08-28 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the universal detection of bacteria and eucarya

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976791A (en) * 1994-08-24 1999-11-02 Bio Merieux Nucleotide fragments capable of hybridizing specifically to rickettsia rDNA or rRNA and their use as probes or primers
US5786147A (en) * 1995-09-18 1998-07-28 Bio Merieux Detection of enterobacteria
EP0786519A2 (fr) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Polynucléotides et séquences de Staphylococcus aureus
US6280946B2 (en) * 1998-08-07 2001-08-28 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the universal detection of bacteria and eucarya
FR2798386A1 (fr) * 1999-09-10 2001-03-16 Didier Raoult Oligonucleotides monocatenaires, sondes, amorces et procede de detection des spirochetes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BALAYEVA N M ET AL: "Genomic identification of Rickettsia slovaca among spotted fever group rickettsia isolates from Dermacentor marginatus in Armenia.", ACTA VIROLOGICA, vol. 38, no. 6, 1994, pages 321 - 325, XP008005536, ISSN: 0001-723X *
EREMEEVA MARINA ET AL: "Differentiation among spotted fever group rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 32, no. 3, 1994, pages 803 - 810, XP008005537, ISSN: 0095-1137 *
HELLYER T J ET AL: "Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 37, no. 2, February 1999 (1999-02-01), pages 290 - 295, XP002205002, ISSN: 0095-1137 *
HIGGINS ET AL: "Rickettsia felis: a new species of pathogenic Rickettsia isolated from cat fleas", JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 34, no. 3, March 1996 (1996-03-01), pages 671 - 674, XP002145966, ISSN: 0095-1137 *

Also Published As

Publication number Publication date
WO2003035905A2 (fr) 2003-05-01
FR2831187A1 (fr) 2003-04-25

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