WO2003033740A3 - Appareil et procede pour isoler un acide nucleique - Google Patents

Appareil et procede pour isoler un acide nucleique Download PDF

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Publication number
WO2003033740A3
WO2003033740A3 PCT/US2002/021715 US0221715W WO03033740A3 WO 2003033740 A3 WO2003033740 A3 WO 2003033740A3 US 0221715 W US0221715 W US 0221715W WO 03033740 A3 WO03033740 A3 WO 03033740A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
porous support
sample
component
amplification
Prior art date
Application number
PCT/US2002/021715
Other languages
English (en)
Other versions
WO2003033740A2 (fr
Inventor
Lalitha Parameswaran
Albert Young
Laura T Bortolin
Mark A Hollis
James Harper
Johanna Bobrow
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Publication of WO2003033740A2 publication Critical patent/WO2003033740A2/fr
Publication of WO2003033740A3 publication Critical patent/WO2003033740A3/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0282—Burettes; Pipettes mounted within a receptacle
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Procédé pour préparer un composant d'acide nucléique d'un échantillon à l'amplification; il consiste à mettre l'échantillon avec un support poreux qui désactive un composant inhibiteur d'amplification d'acide nucléique de l'échantillon et dirige un fluide à travers le support poreux. Le composant d'acide nucléique de l'échantillon passe à travers au moins une partie du support poreux et est séparé du support, ce qui prépare le composant d'acide nucléique à l'amplification. Le procédé peut être mis en oeuvre dans un appareil qui comprend un support poreux comprenant un composant qui désactive un composant inhibiteur d'amplification d'acide nucléique d'un échantillon en contact avec le support poreux et un boîtier possédant une ouverture et définissant un intérieur, ledit intérieur étant en communication fluidique avec le support poreux. On fait passer au moins une partie d'un fluide circulant par l'ouverture à travers au moins une partie du support poreux; elle sépare au moins une partie d'un composant d'acide nucléique d'un échantillon en contact avec le support poreux dudit support, ce qui prépare le composant d'acide nucléique à l'amplification.
PCT/US2002/021715 2001-07-10 2002-07-10 Appareil et procede pour isoler un acide nucleique WO2003033740A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30460801P 2001-07-10 2001-07-10
US60/304,608 2001-07-10

Publications (2)

Publication Number Publication Date
WO2003033740A2 WO2003033740A2 (fr) 2003-04-24
WO2003033740A3 true WO2003033740A3 (fr) 2003-09-25

Family

ID=23177210

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021715 WO2003033740A2 (fr) 2001-07-10 2002-07-10 Appareil et procede pour isoler un acide nucleique

Country Status (2)

Country Link
US (1) US7217513B2 (fr)
WO (1) WO2003033740A2 (fr)

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Publication number Priority date Publication date Assignee Title
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Also Published As

Publication number Publication date
WO2003033740A2 (fr) 2003-04-24
US20030129614A1 (en) 2003-07-10
US7217513B2 (en) 2007-05-15

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