WO2003025113A3 - Verfahren zur kultivierung und analyse mikrobieller einzelzellkulturen - Google Patents

Verfahren zur kultivierung und analyse mikrobieller einzelzellkulturen Download PDF

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Publication number
WO2003025113A3
WO2003025113A3 PCT/DE2002/003451 DE0203451W WO03025113A3 WO 2003025113 A3 WO2003025113 A3 WO 2003025113A3 DE 0203451 W DE0203451 W DE 0203451W WO 03025113 A3 WO03025113 A3 WO 03025113A3
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WO
WIPO (PCT)
Prior art keywords
methods
individual cells
cultivating
microorganisms
cell cultures
Prior art date
Application number
PCT/DE2002/003451
Other languages
English (en)
French (fr)
Other versions
WO2003025113A2 (de
Inventor
Peter-Juergen Mueller
Martin Roth
Ingrid Groth
Christel Kummer
Karin Martin
Volker Schroeckh
Josef Metze
Michael Koehler
Thomas Henkel
Gunter Gastrock
Karen Lemke
Matthias Di Hilliger
Original Assignee
Knoell Hans Forschung Ev
Inst Bioprozess Analysenmesst
Inst Physikalische Hochtech Ev
Peter-Juergen Mueller
Martin Roth
Hilliger Monika Hf
Hilliger Stephan Hm
Ingrid Groth
Christel Kummer
Karin Martin
Volker Schroeckh
Josef Metze
Michael Koehler
Thomas Henkel
Gunter Gastrock
Karen Lemke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Knoell Hans Forschung Ev, Inst Bioprozess Analysenmesst, Inst Physikalische Hochtech Ev, Peter-Juergen Mueller, Martin Roth, Hilliger Monika Hf, Hilliger Stephan Hm, Ingrid Groth, Christel Kummer, Karin Martin, Volker Schroeckh, Josef Metze, Michael Koehler, Thomas Henkel, Gunter Gastrock, Karen Lemke filed Critical Knoell Hans Forschung Ev
Priority to AU2002339300A priority Critical patent/AU2002339300A1/en
Priority to DE50209027T priority patent/DE50209027D1/de
Priority to JP2003529888A priority patent/JP2005502378A/ja
Priority to US10/489,141 priority patent/US20050084923A1/en
Priority to EP02776674A priority patent/EP1425384B1/de
Publication of WO2003025113A2 publication Critical patent/WO2003025113A2/de
Publication of WO2003025113A3 publication Critical patent/WO2003025113A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/16—Solid state fermenters, e.g. for koji production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft Verfahren zur Vereinzelung der Mikroorganismen aus einer Suspension oder Kultur, der parallelen Kultivierung der vereinzelten Zellen und der Analyse ihrer Stoffwechselleistungen. Mit der Erfindung können mikrobielle Grundoperationen wie Medienoptimierung, Screening nach Bildern neuer Naturstoffe und spezieller Stoffwechselleistungen, qualitativer und quantitativer Nachweis der Wirkungen von Nährsubstraten, Effektoren und Wirkstoffen einschliesslich der Medienoptimierung sowie die Selektion von Mikroorganismenklonen mit bestimmten Eigenschaften aus hochzahligen Populationen nach Mutagenese, Transformation, Transfektion und gentechnischer Bearbeitung sowie der Nachweis von mikrobiellen Kontaminationen durchgeführt werden. Die Erfindung ist mit besonderem Vorteil immer dann anwendbar, wenn aus einer vielzahligen Population Mikroorganismen mit herausragenden Eigenschaften jeweils als Einzelzellen oder Einzelorganismen gewonnen und als Reinkulturen charakterisiert werden sollen, oder wenn die Wirkung von Einflussgrössen an vielen, untereinander vollständig vergleichbaren Kulturen untersucht werden soll.
PCT/DE2002/003451 2001-09-14 2002-09-13 Verfahren zur kultivierung und analyse mikrobieller einzelzellkulturen WO2003025113A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2002339300A AU2002339300A1 (en) 2001-09-14 2002-09-13 Methods for cultivating and analyzing microbial individual cell cultures
DE50209027T DE50209027D1 (de) 2001-09-14 2002-09-13 Verfahren zur kultivierung und analyse mikrobieller einzelzellkulturen
JP2003529888A JP2005502378A (ja) 2001-09-14 2002-09-13 微生物個別細胞培養物の培養方法及び解析方法
US10/489,141 US20050084923A1 (en) 2001-09-14 2002-09-13 Methods for cultivating and analyzing microbial individual cell cultures
EP02776674A EP1425384B1 (de) 2001-09-14 2002-09-13 Verfahren zur kultivierung und analyse mikrobieller einzelzellkulturen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10145568.2 2001-09-14
DE10145568A DE10145568A1 (de) 2001-09-14 2001-09-14 Verfahren zur Kultivierung und Analyse mikrobieller Einzelzellkulturen

Publications (2)

Publication Number Publication Date
WO2003025113A2 WO2003025113A2 (de) 2003-03-27
WO2003025113A3 true WO2003025113A3 (de) 2003-06-26

Family

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PCT/DE2002/003451 WO2003025113A2 (de) 2001-09-14 2002-09-13 Verfahren zur kultivierung und analyse mikrobieller einzelzellkulturen

Country Status (7)

Country Link
US (1) US20050084923A1 (de)
EP (1) EP1425384B1 (de)
JP (1) JP2005502378A (de)
AT (1) ATE348875T1 (de)
AU (1) AU2002339300A1 (de)
DE (2) DE10145568A1 (de)
WO (1) WO2003025113A2 (de)

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Also Published As

Publication number Publication date
US20050084923A1 (en) 2005-04-21
EP1425384A2 (de) 2004-06-09
AU2002339300A1 (en) 2003-04-01
JP2005502378A (ja) 2005-01-27
DE50209027D1 (de) 2007-02-01
DE10145568A1 (de) 2003-04-03
WO2003025113A2 (de) 2003-03-27
ATE348875T1 (de) 2007-01-15
EP1425384B1 (de) 2006-12-20

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