WO2003020745A3 - Generic, parallel and scaleable protein purification - Google Patents

Generic, parallel and scaleable protein purification Download PDF

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Publication number
WO2003020745A3
WO2003020745A3 PCT/EP2002/009737 EP0209737W WO03020745A3 WO 2003020745 A3 WO2003020745 A3 WO 2003020745A3 EP 0209737 W EP0209737 W EP 0209737W WO 03020745 A3 WO03020745 A3 WO 03020745A3
Authority
WO
WIPO (PCT)
Prior art keywords
matrix
fusion protein
protein complex
mixture
scaleable
Prior art date
Application number
PCT/EP2002/009737
Other languages
French (fr)
Other versions
WO2003020745A2 (en
Inventor
Kerstin Blank
Peter Lindner
Andreas Plueckthun
Original Assignee
Univ Zuerich
Kerstin Blank
Peter Lindner
Andreas Plueckthun
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Zuerich, Kerstin Blank, Peter Lindner, Andreas Plueckthun filed Critical Univ Zuerich
Priority to AU2002337050A priority Critical patent/AU2002337050A1/en
Publication of WO2003020745A2 publication Critical patent/WO2003020745A2/en
Publication of WO2003020745A3 publication Critical patent/WO2003020745A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a method of separating a ligand comprised within a mixture comprising the steps of: (a) immobilizing a fusion protein on a matrix, said fusion protein comprising (i) an antibody fragment moiety having a specificity for said ligand and (ii) an affinity domain moiety that attaches to the matrix to form a matrix-fusion protein complex; (b) contacting said mixture with said matrix-fusion protein complex; (c) removing components of said mixture not binding to the antibody fragment moiety of said matrix-fusion protein complex; and (d) eluting said ligand from said matrix-fusion protein complex. The invention furthermore relates to fusion proteins comprising two or more chitin-binding domains, to uses thereof, and to affinity chips and their application.
PCT/EP2002/009737 2001-09-04 2002-08-30 Generic, parallel and scaleable protein purification WO2003020745A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002337050A AU2002337050A1 (en) 2001-09-04 2002-08-30 Generic, parallel and scaleable protein purification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01120712.3 2001-09-04
EP01120712 2001-09-04

Publications (2)

Publication Number Publication Date
WO2003020745A2 WO2003020745A2 (en) 2003-03-13
WO2003020745A3 true WO2003020745A3 (en) 2003-10-09

Family

ID=8178458

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/009737 WO2003020745A2 (en) 2001-09-04 2002-08-30 Generic, parallel and scaleable protein purification

Country Status (2)

Country Link
AU (1) AU2002337050A1 (en)
WO (1) WO2003020745A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2634547T3 (en) 2010-08-26 2017-09-28 Agrosavfe N.V. Compositions for seed treatment

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021331A2 (en) * 1992-04-08 1993-10-28 University Of British Columbia Polysaccharide binding fusion proteins and conjugates
US5258502A (en) * 1989-01-30 1993-11-02 Massachusetts Institute Of Technology Immobilization and purification of fusion proteins using chitin-binding ability
US5719044A (en) * 1993-04-14 1998-02-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Cellulose binding domain fusion proteins

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258502A (en) * 1989-01-30 1993-11-02 Massachusetts Institute Of Technology Immobilization and purification of fusion proteins using chitin-binding ability
WO1993021331A2 (en) * 1992-04-08 1993-10-28 University Of British Columbia Polysaccharide binding fusion proteins and conjugates
US5719044A (en) * 1993-04-14 1998-02-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Cellulose binding domain fusion proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BLANK KERSTIN ET AL: "Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: Generic, parallel, and scalable protein purification.", PROTEIN EXPRESSION AND PURIFICATION, vol. 24, no. 2, March 2002 (2002-03-01), March, 2002, pages 313 - 322, XP002237767, ISSN: 1046-5928 *

Also Published As

Publication number Publication date
AU2002337050A1 (en) 2003-03-18
WO2003020745A2 (en) 2003-03-13

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