WO2003019158A3 - Thermo-optisches analysesystem für biochemische reaktionen - Google Patents

Thermo-optisches analysesystem für biochemische reaktionen Download PDF

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Publication number
WO2003019158A3
WO2003019158A3 PCT/EP2002/009340 EP0209340W WO03019158A3 WO 2003019158 A3 WO2003019158 A3 WO 2003019158A3 EP 0209340 W EP0209340 W EP 0209340W WO 03019158 A3 WO03019158 A3 WO 03019158A3
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WO
WIPO (PCT)
Prior art keywords
light source
reaction
electromagnetic radiation
unit
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/009340
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English (en)
French (fr)
Other versions
WO2003019158A2 (de
Inventor
Daniel Baechi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to US10/485,819 priority Critical patent/US7521179B2/en
Priority to EP02796255A priority patent/EP1419374A2/de
Priority to AU2002333497A priority patent/AU2002333497A1/en
Publication of WO2003019158A2 publication Critical patent/WO2003019158A2/de
Publication of WO2003019158A3 publication Critical patent/WO2003019158A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0332—Cuvette constructions with temperature control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Die vorliegende Erfindung betrifft eine Reaktionsvorrichtung, umfassend eine Probenkammermatrix (1), enthaltend mindestens zwei Reaktionskammern (2), deren Wände für elektromagnetische Strahlung in einem für Fluoreszenzmessung erforderlichen Bereich durchlässig sind; mindestens eine Öffnung (3); eine Aufheizeinrichtung (8a); und eine Temperaturmesseinrichtung (8b). Die vorliegende Erfindung betrifft weiterhin ein Gerät (G) zur Durchführung und Detektion chemischer Reaktionen, umfassend mindestens eine Lichtquelle (24, 25), welche elektromagnetische Strahlung in einem für Fluoreszenzmessung erforderlichen Bereich emittiert; eine Erfassungseinheit (28) zur Erfassung von elektromagnetischer Strahlung in einem für Fluoreszenzmessung erforderlichen Bereich, wobei diese Einheit (28) im rechten Winkel zu der mindestens einen Lichtquelle (24, 25) angeordnet ist; eine Steuereinheit umfassend eine Zentraleinheit zur Steuerung der Temperatur und der mindestens einen Lichtquelle (24, 25) sowie zur Aufnahme und gegebenenfalls Verarbeitung eines Signals von der Erfassungseinheit (28); eine Öffnung (H) zur Aufnahme der vorstehenden Reaktionsvorrichtung (R), wobei die Reaktionsvorrichtung (R) in eingeführtem Zustand mit der Steuereinheit kommunizieren kann, sich in der Ebene der mindestens einen Lichtquelle (24, 25) und unterhalb der Erfassungseinheit (28) befindet. Die Reaktionsvorrichtung (R) und das Gerät (G) eignen sich zur Durchführung und Auswertung chemischer Reaktionen oder biochemischer Reaktionen wie der Polymerase-Kettenreaktion.
PCT/EP2002/009340 2001-08-21 2002-08-21 Thermo-optisches analysesystem für biochemische reaktionen Ceased WO2003019158A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/485,819 US7521179B2 (en) 2001-08-21 2002-08-21 Thermo-optical analysis system for biological reactions
EP02796255A EP1419374A2 (de) 2001-08-21 2002-08-21 Thermo-optisches analysesystem für biochemische reaktionen
AU2002333497A AU2002333497A1 (en) 2001-08-21 2002-08-21 Thermo-optical analysis system for biochemical reactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH15442001 2001-08-21
CH20011544/01 2001-08-21

Publications (2)

Publication Number Publication Date
WO2003019158A2 WO2003019158A2 (de) 2003-03-06
WO2003019158A3 true WO2003019158A3 (de) 2003-11-13

Family

ID=4565547

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/009340 Ceased WO2003019158A2 (de) 2001-08-21 2002-08-21 Thermo-optisches analysesystem für biochemische reaktionen

Country Status (4)

Country Link
US (1) US7521179B2 (de)
EP (1) EP1419374A2 (de)
AU (1) AU2002333497A1 (de)
WO (1) WO2003019158A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1703981A1 (de) * 2004-01-12 2006-09-27 Applera Corporation Verfahren und vorrichtung zum nachweis von nucleinsäuresequenzen
DE102005027555B3 (de) * 2005-06-14 2006-10-05 Eppendorf Ag Thermocycler
US7569382B2 (en) * 2005-10-05 2009-08-04 Instantlabs Medical Diagnostic Corp. Disposable reactor module and detection system
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
EP2108451A1 (de) * 2008-04-11 2009-10-14 Eppendorf AG Vorrichtung zum Durchführen von Reaktionen in Proben
FI20096021A0 (fi) 2009-10-06 2009-10-06 Wallac Oy Optinen mittausinstrumentti
CN103221529B (zh) 2010-02-23 2015-05-27 卢米尼克斯公司 用于整合的样品制备、反应和检测的器械和方法
US9096892B1 (en) 2011-02-04 2015-08-04 K2 Biomicrosystems, LLC Nucleic acid amplification and monitoring apparatus
WO2012151473A2 (en) 2011-05-04 2012-11-08 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
CN103969236A (zh) * 2014-05-07 2014-08-06 华中科技大学 一种便携式病原体检测仪
DE102015100278B3 (de) * 2015-01-09 2016-05-04 Rheotec Messtechnik Gmbh Temperieranordnung mit Kombinationswärmeübertrager zur Heizung und Kühlung einer Messplatte eines Messgerätes
DE102015100272B3 (de) * 2015-01-09 2016-05-04 Rheotec Messtechnik Gmbh Temperieranordnung für Messgeräte
WO2016115542A1 (en) * 2015-01-16 2016-07-21 The Regents Of The University Of California Led driven plasmonic heating apparatus for nucleic acids amplification

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4038151A (en) * 1976-07-29 1977-07-26 Mcdonnell Douglas Corporation Card for use in an automated microbial detection system
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5681484A (en) * 1994-11-10 1997-10-28 David Sarnoff Research Center, Inc. Etching to form cross-over, non-intersecting channel networks for use in partitioned microelectronic and fluidic device arrays for clinical diagnostics and chemical synthesis
WO1999046045A1 (de) * 1998-03-11 1999-09-16 MICROPARTS GESELLSCHAFT FüR MIKROSTRUKTURTECHNIK MBH Probenträger
WO2002029397A2 (en) * 2000-10-05 2002-04-11 E.I. Du Pont De Nemours And Company Polymeric microfabricated fluidic device suitable for ultraviolet detection

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
WO1998022625A1 (en) * 1996-11-20 1998-05-28 The Regents Of The University Of Michigan Microfabricated isothermal nucleic acid amplification devices and methods
US6369893B1 (en) * 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
JP2000304689A (ja) * 1999-04-21 2000-11-02 Hiroyuki Ogawa 投影観察方法、微生物検査方法および投影検出装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4038151A (en) * 1976-07-29 1977-07-26 Mcdonnell Douglas Corporation Card for use in an automated microbial detection system
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5681484A (en) * 1994-11-10 1997-10-28 David Sarnoff Research Center, Inc. Etching to form cross-over, non-intersecting channel networks for use in partitioned microelectronic and fluidic device arrays for clinical diagnostics and chemical synthesis
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
WO1999046045A1 (de) * 1998-03-11 1999-09-16 MICROPARTS GESELLSCHAFT FüR MIKROSTRUKTURTECHNIK MBH Probenträger
WO2002029397A2 (en) * 2000-10-05 2002-04-11 E.I. Du Pont De Nemours And Company Polymeric microfabricated fluidic device suitable for ultraviolet detection

Also Published As

Publication number Publication date
US20040241691A1 (en) 2004-12-02
AU2002333497A1 (en) 2003-03-10
EP1419374A2 (de) 2004-05-19
US7521179B2 (en) 2009-04-21
WO2003019158A2 (de) 2003-03-06

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