WO2003019158A3 - Thermo-optisches analysesystem für biochemische reaktionen - Google Patents

Thermo-optisches analysesystem für biochemische reaktionen Download PDF

Info

Publication number
WO2003019158A3
WO2003019158A3 PCT/EP2002/009340 EP0209340W WO03019158A3 WO 2003019158 A3 WO2003019158 A3 WO 2003019158A3 EP 0209340 W EP0209340 W EP 0209340W WO 03019158 A3 WO03019158 A3 WO 03019158A3
Authority
WO
WIPO (PCT)
Prior art keywords
light source
reaction
electromagnetic radiation
unit
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/009340
Other languages
English (en)
French (fr)
Other versions
WO2003019158A2 (de
Inventor
Daniel Baechi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU2002333497A priority Critical patent/AU2002333497A1/en
Priority to EP02796255A priority patent/EP1419374A2/de
Priority to US10/485,819 priority patent/US7521179B2/en
Publication of WO2003019158A2 publication Critical patent/WO2003019158A2/de
Publication of WO2003019158A3 publication Critical patent/WO2003019158A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0332—Cuvette constructions with temperature control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Optics & Photonics (AREA)
  • Molecular Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Die vorliegende Erfindung betrifft eine Reaktionsvorrichtung, umfassend eine Probenkammermatrix (1), enthaltend mindestens zwei Reaktionskammern (2), deren Wände für elektromagnetische Strahlung in einem für Fluoreszenzmessung erforderlichen Bereich durchlässig sind; mindestens eine Öffnung (3); eine Aufheizeinrichtung (8a); und eine Temperaturmesseinrichtung (8b). Die vorliegende Erfindung betrifft weiterhin ein Gerät (G) zur Durchführung und Detektion chemischer Reaktionen, umfassend mindestens eine Lichtquelle (24, 25), welche elektromagnetische Strahlung in einem für Fluoreszenzmessung erforderlichen Bereich emittiert; eine Erfassungseinheit (28) zur Erfassung von elektromagnetischer Strahlung in einem für Fluoreszenzmessung erforderlichen Bereich, wobei diese Einheit (28) im rechten Winkel zu der mindestens einen Lichtquelle (24, 25) angeordnet ist; eine Steuereinheit umfassend eine Zentraleinheit zur Steuerung der Temperatur und der mindestens einen Lichtquelle (24, 25) sowie zur Aufnahme und gegebenenfalls Verarbeitung eines Signals von der Erfassungseinheit (28); eine Öffnung (H) zur Aufnahme der vorstehenden Reaktionsvorrichtung (R), wobei die Reaktionsvorrichtung (R) in eingeführtem Zustand mit der Steuereinheit kommunizieren kann, sich in der Ebene der mindestens einen Lichtquelle (24, 25) und unterhalb der Erfassungseinheit (28) befindet. Die Reaktionsvorrichtung (R) und das Gerät (G) eignen sich zur Durchführung und Auswertung chemischer Reaktionen oder biochemischer Reaktionen wie der Polymerase-Kettenreaktion.
PCT/EP2002/009340 2001-08-21 2002-08-21 Thermo-optisches analysesystem für biochemische reaktionen Ceased WO2003019158A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002333497A AU2002333497A1 (en) 2001-08-21 2002-08-21 Thermo-optical analysis system for biochemical reactions
EP02796255A EP1419374A2 (de) 2001-08-21 2002-08-21 Thermo-optisches analysesystem für biochemische reaktionen
US10/485,819 US7521179B2 (en) 2001-08-21 2002-08-21 Thermo-optical analysis system for biological reactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH20011544/01 2001-08-21
CH15442001 2001-08-21

Publications (2)

Publication Number Publication Date
WO2003019158A2 WO2003019158A2 (de) 2003-03-06
WO2003019158A3 true WO2003019158A3 (de) 2003-11-13

Family

ID=4565547

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/009340 Ceased WO2003019158A2 (de) 2001-08-21 2002-08-21 Thermo-optisches analysesystem für biochemische reaktionen

Country Status (4)

Country Link
US (1) US7521179B2 (de)
EP (1) EP1419374A2 (de)
AU (1) AU2002333497A1 (de)
WO (1) WO2003019158A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1703981A1 (de) * 2004-01-12 2006-09-27 Applera Corporation Verfahren und vorrichtung zum nachweis von nucleinsäuresequenzen
DE102005027555B3 (de) * 2005-06-14 2006-10-05 Eppendorf Ag Thermocycler
US7569382B2 (en) * 2005-10-05 2009-08-04 Instantlabs Medical Diagnostic Corp. Disposable reactor module and detection system
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
US8372340B2 (en) 2005-10-19 2013-02-12 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
EP2108451A1 (de) * 2008-04-11 2009-10-14 Eppendorf AG Vorrichtung zum Durchführen von Reaktionen in Proben
FI20096021A0 (fi) 2009-10-06 2009-10-06 Wallac Oy Optinen mittausinstrumentti
US9096892B1 (en) 2011-02-04 2015-08-04 K2 Biomicrosystems, LLC Nucleic acid amplification and monitoring apparatus
CN104023834B (zh) 2011-05-04 2016-09-28 卢米耐克斯公司 用于集成的样品制备、反应和检测的设备与方法
CN103969236A (zh) * 2014-05-07 2014-08-06 华中科技大学 一种便携式病原体检测仪
DE102015100278B3 (de) * 2015-01-09 2016-05-04 Rheotec Messtechnik Gmbh Temperieranordnung mit Kombinationswärmeübertrager zur Heizung und Kühlung einer Messplatte eines Messgerätes
DE102015100272B3 (de) * 2015-01-09 2016-05-04 Rheotec Messtechnik Gmbh Temperieranordnung für Messgeräte
WO2016115542A1 (en) * 2015-01-16 2016-07-21 The Regents Of The University Of California Led driven plasmonic heating apparatus for nucleic acids amplification

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4038151A (en) * 1976-07-29 1977-07-26 Mcdonnell Douglas Corporation Card for use in an automated microbial detection system
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5681484A (en) * 1994-11-10 1997-10-28 David Sarnoff Research Center, Inc. Etching to form cross-over, non-intersecting channel networks for use in partitioned microelectronic and fluidic device arrays for clinical diagnostics and chemical synthesis
WO1999046045A1 (de) * 1998-03-11 1999-09-16 MICROPARTS GESELLSCHAFT FüR MIKROSTRUKTURTECHNIK MBH Probenträger
WO2002029397A2 (en) * 2000-10-05 2002-04-11 E.I. Du Pont De Nemours And Company Polymeric microfabricated fluidic device suitable for ultraviolet detection

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US6379929B1 (en) * 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
US6369893B1 (en) * 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
JP2000304689A (ja) * 1999-04-21 2000-11-02 Hiroyuki Ogawa 投影観察方法、微生物検査方法および投影検出装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4038151A (en) * 1976-07-29 1977-07-26 Mcdonnell Douglas Corporation Card for use in an automated microbial detection system
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5681484A (en) * 1994-11-10 1997-10-28 David Sarnoff Research Center, Inc. Etching to form cross-over, non-intersecting channel networks for use in partitioned microelectronic and fluidic device arrays for clinical diagnostics and chemical synthesis
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
WO1999046045A1 (de) * 1998-03-11 1999-09-16 MICROPARTS GESELLSCHAFT FüR MIKROSTRUKTURTECHNIK MBH Probenträger
WO2002029397A2 (en) * 2000-10-05 2002-04-11 E.I. Du Pont De Nemours And Company Polymeric microfabricated fluidic device suitable for ultraviolet detection

Also Published As

Publication number Publication date
WO2003019158A2 (de) 2003-03-06
AU2002333497A1 (en) 2003-03-10
EP1419374A2 (de) 2004-05-19
US7521179B2 (en) 2009-04-21
US20040241691A1 (en) 2004-12-02

Similar Documents

Publication Publication Date Title
US12091711B2 (en) Methods and systems for nucleic acid analysis and quantification
WO2003019158A3 (de) Thermo-optisches analysesystem für biochemische reaktionen
CA2525332C (en) Systems and methods for fluorescence detection with a movable detection module
AU4505797A (en) Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and use for cellular assays in drug discovery
WO2003064993A3 (en) Methods and materials for detecting genetic material
WO2007141700A3 (en) Microelectronic sensor device for dna detection
JP2005538728A5 (de)
WO2005118773A3 (en) Apparatus and methods for multiplex analyses
CN103221529A (zh) 用于整合的样品制备、反应和检测的器械和方法
IL145459A0 (en) Individually addressable micro-electromagnetic unit array chips
WO2005028629A3 (en) Whole genome expression analysis system
WO2006119277A3 (en) System and method for a pulsed light source used in fluorescence detection
JP2003344290A (ja) 温度調節付蛍光検出装置
KR102666561B1 (ko) 다채널 등온 증폭 시스템
WO2003011768A3 (en) Microfluidic device for molecular analysis
KR20230043442A (ko) 다채널 등온 증폭 방법 및 시스템
JP7797537B2 (ja) 自動化された自己完結型生物学的分析のためのシステム、方法、および装置
Lee et al. Development of a CCD-based fluorimeter for real-time PCR machine
CA2237539C (en) Integrally attached and operable multiple reaction vessels
Csordas et al. Nucleic acid sensor and fluid handling for detection of bacterial pathogens
Wang et al. A portable generic DNA bioassay system based on in situ oligonucleotide synthesis and hybridization detection
KR20220122244A (ko) 휴대용 등온증폭장치
KR20240060469A (ko) 광 검출 모듈 및 이를 구비하는 타깃 분석물 검출 장치
WO2002073172A3 (de) Bestimmung von analyten durch fluoreszenz-korrelationsspektroskopie

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BY BZ CA CH CN CO CR CU CZ DE DM DZ EC EE ES FI GB GD GE GH HR HU ID IL IN IS JP KE KG KP KR LC LK LR LS LT LU LV MA MD MG MN MW MX MZ NO NZ OM PH PL PT RU SD SE SG SI SK SL TJ TM TN TR TZ UA UG US UZ VN YU ZA ZM

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ UG ZM ZW AM AZ BY KG KZ RU TJ TM AT BE BG CH CY CZ DK EE ES FI FR GB GR IE IT LU MC PT SE SK TR BF BJ CF CG CI GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002796255

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10485819

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2002796255

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP