WO2003012076A3 - Culture medium to improve the purity of myoblast cultures and method using same - Google Patents

Culture medium to improve the purity of myoblast cultures and method using same Download PDF

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Publication number
WO2003012076A3
WO2003012076A3 PCT/CA2002/001216 CA0201216W WO03012076A3 WO 2003012076 A3 WO2003012076 A3 WO 2003012076A3 CA 0201216 W CA0201216 W CA 0201216W WO 03012076 A3 WO03012076 A3 WO 03012076A3
Authority
WO
WIPO (PCT)
Prior art keywords
relates
myoblasts
improve
present
culture medium
Prior art date
Application number
PCT/CA2002/001216
Other languages
French (fr)
Other versions
WO2003012076A2 (en
Inventor
Jacques P Tremblay
Original Assignee
Univ Laval
Jacques P Tremblay
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Laval, Jacques P Tremblay filed Critical Univ Laval
Priority to AU2002322245A priority Critical patent/AU2002322245A1/en
Publication of WO2003012076A2 publication Critical patent/WO2003012076A2/en
Publication of WO2003012076A3 publication Critical patent/WO2003012076A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)

Abstract

The present invention relates to a novel culture medium composition to improve the percentage of myoblasts in a primary culture made from a muscle biopsy, which in turn permits a more successful treatment of myopathies and cardiopathies. The present invention also relates to a method to increase the preparation of myoblasts in a primary culture of mixed cells and in particular from a muscle biopsy. The invention also relates to such enriched myoblast cultures to treat myopathies and cardiopathies. In addition, the invention relates to a nutrient medium which increases the proportion of myoblasts with respect to other cell types. The present invention also relates to a myoblast basal growth medium comprising all nutrients required for growth and expansion of myoblasts wherein at least a portion of the amino acid L-valine is replaced with D-valine.
PCT/CA2002/001216 2001-08-02 2002-08-02 Culture medium to improve the purity of myoblast cultures and method using same WO2003012076A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002322245A AU2002322245A1 (en) 2001-08-02 2002-08-02 Culture medium to improve the purity of myoblast cultures and method using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA 2354978 CA2354978A1 (en) 2001-08-02 2001-08-02 Culture medium to improve the purity of myoblast culture
CA2,354,978 2001-08-02

Publications (2)

Publication Number Publication Date
WO2003012076A2 WO2003012076A2 (en) 2003-02-13
WO2003012076A3 true WO2003012076A3 (en) 2003-05-30

Family

ID=4169713

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/001216 WO2003012076A2 (en) 2001-08-02 2002-08-02 Culture medium to improve the purity of myoblast cultures and method using same

Country Status (3)

Country Link
AU (1) AU2002322245A1 (en)
CA (1) CA2354978A1 (en)
WO (1) WO2003012076A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2235640B1 (en) * 2003-12-17 2006-12-16 Consejo Sup. Investig. Cientificas MEANS OF CULTURE OF HUMAN PROGENITING CELLS AND USE FOR THE PROLIFERATION OF SUCH CELLS FOR THEIR AUTOMOTIVE TRANSPLANT.
CN109153963B (en) 2016-11-01 2022-06-24 泰尔茂株式会社 Method for changing cell culture in adhesion state

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324656A (en) * 1988-11-01 1994-06-28 University Of Colorado Foundation, Inc. Media for normal human muscle satellite cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324656A (en) * 1988-11-01 1994-06-28 University Of Colorado Foundation, Inc. Media for normal human muscle satellite cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BOUDREAULT PASCALE ET AL: "Scale-up of a myoblast culture process.", JOURNAL OF BIOTECHNOLOGY, vol. 91, no. 1, 2001, pages 63 - 74, XP002228060, ISSN: 0168-1656 *
HONGPAISAN JARIN: "Inhibition of proliferation of contaminating fibroblasts by D-valine in cultures of smooth muscle cells from human myometrium", CELL BIOLOGY INTERNATIONAL, ACADEMIC PRESS, GB, vol. 24, no. 1, May 2000 (2000-05-01), pages 1 - 7, XP002175030, ISSN: 1065-6995 *
LAZARO V A ET AL: "INHIBITION OF FIBROBLAST PROLIFERATION IN L VALINE REDUCED SELECTIVE MEDIA", RESEARCH COMMUNICATIONS IN CHEMICAL PATHOLOGY AND PHARMACOLOGY, vol. 75, no. 1, 1992, pages 39 - 48, XP009004518, ISSN: 0034-5164 *
QU ZHUQING ET AL: "Development of approaches to improve cell survival in myoblast transfer therapy", THE JOURNAL OF CELL BIOLOGY, ROCKEFELLER UNIVERSITY PRESS, US, vol. 142, no. 5, 7 September 1998 (1998-09-07), pages 1257 - 1267, XP002161312, ISSN: 0021-9525 *

Also Published As

Publication number Publication date
CA2354978A1 (en) 2003-02-02
WO2003012076A2 (en) 2003-02-13
AU2002322245A1 (en) 2003-02-17

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