WO2003010332A3 - Test-kit for detecting enterohemorrhagic $i(escherichia coli) strains (ehec) - Google Patents

Test-kit for detecting enterohemorrhagic $i(escherichia coli) strains (ehec) Download PDF

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Publication number
WO2003010332A3
WO2003010332A3 PCT/AT2002/000222 AT0200222W WO03010332A3 WO 2003010332 A3 WO2003010332 A3 WO 2003010332A3 AT 0200222 W AT0200222 W AT 0200222W WO 03010332 A3 WO03010332 A3 WO 03010332A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
escherichia coli
test
kit
ehec
Prior art date
Application number
PCT/AT2002/000222
Other languages
German (de)
French (fr)
Other versions
WO2003010332A2 (en
Inventor
Sabine Vollenhofer-Schrumpf
Manfred Schinkinger
Gert Fraenzl
Original Assignee
Sy Lab Vgmbh
Sabine Vollenhofer-Schrumpf
Manfred Schinkinger
Gert Fraenzl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sy Lab Vgmbh, Sabine Vollenhofer-Schrumpf, Manfred Schinkinger, Gert Fraenzl filed Critical Sy Lab Vgmbh
Priority to EP02762153A priority Critical patent/EP1409742A2/en
Publication of WO2003010332A2 publication Critical patent/WO2003010332A2/en
Publication of WO2003010332A3 publication Critical patent/WO2003010332A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a test-kit for detecting enterohemorrhagic escherichia coli strains (EHEC), containing, each time, a primer pair for amplifying at least a portion of the SLT1 gene, SLT2 gene, r∫bE gene, eaeA gene and of a control gene in a single reaction and containing an oligonucleotide serving as a trapping probe and an oligonucleotide serving as a detector probe for hybridizing, at room temperature, the amplification products that are obtained with the primers. According to the invention, all trapping probes are immobilized on a common solid surface, and the detector probes are provided with a visually detectable marking. The use of a test kit enables a simple and reliable detection of enterohemorrhagic escherichia coli strains in a single reaction.
PCT/AT2002/000222 2001-07-26 2002-07-26 Test-kit for detecting enterohemorrhagic $i(escherichia coli) strains (ehec) WO2003010332A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02762153A EP1409742A2 (en) 2001-07-26 2002-07-26 Test-kit for detecting enterohemorrhagic escherichia coli strains (ehec)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0117201A AT411832B (en) 2001-07-26 2001-07-26 TEST KIT FOR DETECTING THE BACTERIAL GENES SLT1, SLT2 AND RFBE
ATA1172/2001 2001-07-26

Publications (2)

Publication Number Publication Date
WO2003010332A2 WO2003010332A2 (en) 2003-02-06
WO2003010332A3 true WO2003010332A3 (en) 2003-11-06

Family

ID=3686560

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT2002/000222 WO2003010332A2 (en) 2001-07-26 2002-07-26 Test-kit for detecting enterohemorrhagic $i(escherichia coli) strains (ehec)

Country Status (3)

Country Link
EP (1) EP1409742A2 (en)
AT (1) AT411832B (en)
WO (1) WO2003010332A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.
CN100441696C (en) * 2004-10-22 2008-12-10 中国疾病预防控制中心传染病预防控制所 Method for detecting enterohemorrhagic Escherichia coli O157:H7 strain

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999004039A1 (en) * 1995-04-14 1999-01-28 Children's Hospital And Medical Center Methods and materials for detecting e. coli o157 in polymerase chain reaction assays
FR2777907A1 (en) * 1998-04-28 1999-10-29 Pasteur Sanofi Diagnostics New nucleic acid containing sequences specific to enterohemorrhagic Escherichia coli, particularly serotype O157:H7, used for detecting these bacteria in food
DE19946296A1 (en) * 1999-09-28 2001-03-29 Roche Diagnostics Gmbh Multiplex PCR for the detection of EHEC infections

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19814828A1 (en) * 1998-04-02 1999-10-07 Roche Diagnostics Gmbh Nucleic acid amplification assay for detecting viral, bacterial, cellular, yeast or fungal nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999004039A1 (en) * 1995-04-14 1999-01-28 Children's Hospital And Medical Center Methods and materials for detecting e. coli o157 in polymerase chain reaction assays
FR2777907A1 (en) * 1998-04-28 1999-10-29 Pasteur Sanofi Diagnostics New nucleic acid containing sequences specific to enterohemorrhagic Escherichia coli, particularly serotype O157:H7, used for detecting these bacteria in food
DE19946296A1 (en) * 1999-09-28 2001-03-29 Roche Diagnostics Gmbh Multiplex PCR for the detection of EHEC infections

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CALL, D. R. ET AL: "Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2001), 67(1-2), 71-80, XP001153759 *
EDWARDS M C ET AL: "MULTIPLEX PCR: ADVANTAGES, DEVELOPMENT AND APPLICATIONS", PCR METHODS & APPLICATIONS, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 3, no. 4, February 1994 (1994-02-01), pages S65 - S75, XP009003160, ISSN: 1054-9803 *
FORTIN NATHALIE Y ET AL: "Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli O157:H7.", ANALYTICAL BIOCHEMISTRY, vol. 289, no. 2, 15 February 2001 (2001-02-15), pages 281 - 288, XP002250269, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
EP1409742A2 (en) 2004-04-21
AT411832B (en) 2004-06-25
ATA11722001A (en) 2003-11-15
WO2003010332A2 (en) 2003-02-06

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