WO2003004621A3 - Activite de sir2 - Google Patents

Activite de sir2 Download PDF

Info

Publication number
WO2003004621A3
WO2003004621A3 PCT/US2002/021461 US0221461W WO03004621A3 WO 2003004621 A3 WO2003004621 A3 WO 2003004621A3 US 0221461 W US0221461 W US 0221461W WO 03004621 A3 WO03004621 A3 WO 03004621A3
Authority
WO
WIPO (PCT)
Prior art keywords
sir2
activity
screening
compounds
compound
Prior art date
Application number
PCT/US2002/021461
Other languages
English (en)
Other versions
WO2003004621A2 (fr
Inventor
Leonard Guarente
Homayoun Vaziri
Shin-Ichiro Imai
Wei Gu
Original Assignee
Elixir Pharmaceuticals Inc
Leonard Guarente
Homayoun Vaziri
Shin-Ichiro Imai
Wei Gu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elixir Pharmaceuticals Inc, Leonard Guarente, Homayoun Vaziri, Shin-Ichiro Imai, Wei Gu filed Critical Elixir Pharmaceuticals Inc
Priority to EP02749837A priority Critical patent/EP1529115A4/fr
Priority to AU2002320325A priority patent/AU2002320325B2/en
Priority to CA002453195A priority patent/CA2453195A1/fr
Publication of WO2003004621A2 publication Critical patent/WO2003004621A2/fr
Publication of WO2003004621A3 publication Critical patent/WO2003004621A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4703—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne des procédés de criblage de composés modulant des processus cellulaires et organismiques par modification de l'activité de SIR2 et/ou de facteurs de transcription, par exemple, p53 ; notamment, des procédés de criblage de composés modifiant la durée de vie et/ou le métabolisme d'une cellule ou d'un organisme par modulation de l'activité de SIR2 et/ou de facteurs de transcription, par exemple, p53 ; et plus précisément, des procédés de criblage de composés modulant l'activité de Sir2 et/ou de facteurs de transcription, par exemple, p53. Plus précisément, l'invention concerne un procédé de criblage d'un composé consistant à utiliser un mélange d'essai renfermant un facteur de transcription de Sir2 et un cofacteur de Sir2 avec le composé, ainsi qu'à évaluer une activité d'un composant du mélange d'essai en présence du composé. L'invention concerne également des utilisations thérapeutiques desdits composés. L'invention concerne enfin un procédé de modification du statut d'acétylation d'un site de liaison de facteur de transcription sur une histone ou un ADN par élévation des concentrations locales de Sir2.
PCT/US2002/021461 2001-07-06 2002-07-08 Activite de sir2 WO2003004621A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02749837A EP1529115A4 (fr) 2001-07-06 2002-07-08 Activite de sir2
AU2002320325A AU2002320325B2 (en) 2001-07-06 2002-07-08 SIR2 activity
CA002453195A CA2453195A1 (fr) 2001-07-06 2002-07-08 Activite de sir2

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US30337001P 2001-07-06 2001-07-06
US30345601P 2001-07-06 2001-07-06
US60/303,370 2001-07-06
US60/303,456 2001-07-06
US19015902A 2002-07-05 2002-07-05
US10/190,159 2002-07-05

Publications (2)

Publication Number Publication Date
WO2003004621A2 WO2003004621A2 (fr) 2003-01-16
WO2003004621A3 true WO2003004621A3 (fr) 2005-03-17

Family

ID=32074257

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021461 WO2003004621A2 (fr) 2001-07-06 2002-07-08 Activite de sir2

Country Status (4)

Country Link
EP (1) EP1529115A4 (fr)
AU (1) AU2002320325B2 (fr)
CA (1) CA2453195A1 (fr)
WO (1) WO2003004621A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8642284B1 (en) 1999-12-15 2014-02-04 Massachusetts Institute Of Technology Methods for identifying agents that alter NAD-dependent deacetylation activity of a SIR2 protein
US7452664B2 (en) 1999-12-15 2008-11-18 Massachusetts Institute Of Technology Methods for identifying agents which alter histone protein acetylation
US7572575B2 (en) 2000-12-13 2009-08-11 Massachusetts Institute Of Technology SIR2 activity
WO2003046207A2 (fr) 2001-11-27 2003-06-05 Fred Hutchinson Cancer Research Center Procedes d'inhibition de l'activite deacetylase
US7351542B2 (en) 2002-05-20 2008-04-01 The Regents Of The University Of California Methods of modulating tubulin deacetylase activity
DE60335875D1 (de) 2002-05-23 2011-03-10 Univ California Verfahren zum Auswählen von Modulatoren der mitochondrialen NAD-abhängigen Deacetylase, SIRT3
US20070099830A1 (en) 2005-04-21 2007-05-03 Massachusetts Institute Of Technology Sirt4 activities
US8518635B2 (en) 2006-06-12 2013-08-27 The J. David Gladstone Institutes Regulation of protein activity by reversible acetylation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LANDRY ET AL.: "The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases", PNAS, vol. 97, no. 11, 23 May 2000 (2000-05-23), pages 5807 - 5811, XP002963941 *
MARKS ET AL.: "Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells", JOURNAL OF THE NATIONAL CANCER INSTITUTE, vol. 92, no. 15, 2 August 2000 (2000-08-02), pages 1210 - 1216, XP001121734 *
See also references of EP1529115A4 *

Also Published As

Publication number Publication date
AU2002320325B2 (en) 2008-04-03
WO2003004621A2 (fr) 2003-01-16
EP1529115A2 (fr) 2005-05-11
EP1529115A4 (fr) 2005-08-24
CA2453195A1 (fr) 2003-01-16
AU2002320325A2 (en) 2003-01-21

Similar Documents

Publication Publication Date Title
WO2004039941A3 (fr) Procede de criblage de modulateurs trpm4b
WO2005107762A3 (fr) Entites, compositions chimiques et procedes
WO2006058237A3 (fr) Substrats revetus de polymere destines a fixer des biomolecules et procedes de fabrication et d'utilisation correspondants
WO2007030934A3 (fr) Methodes de modulation de l'activite induite par la neurotrophine
WO2006002382A3 (fr) Reseaux de liants clivables
WO2006108087A3 (fr) Dispositifs et procedes permettant d'enrichir et de modifier des cellules tumorales circulantes et d'autres particules
WO2007056143A3 (fr) Entites et compositions chimiques et methodes associees
WO2007056469A3 (fr) Entites et compositions chimiques et methodes associees
WO2005082341A3 (fr) Agents non peptidyl a activite de type phsp20 et utilisations correspondantes
WO2007079229A3 (fr) Dispositifs et procedes d'enrichissement et de modification de cellules tumorales circulantes et d'autres particules
WO2007056078A3 (fr) Entites et compositions chimiques et methodes associees
WO2003004621A3 (fr) Activite de sir2
WO2006042173A3 (fr) Modulateurs d'activateur du facteur de croissance des hepatocytes
WO2005051974A3 (fr) Nouvelles molecules pour la regulation de la mort cellulaire
WO2004076632A3 (fr) Methodes de criblage de modulateurs de trpm5
WO2005023305A3 (fr) Modulation de l'activite cellulaire au moyen d'un agent diminuant le taux de cholesterol au sein d'une cellule
WO2006012594A3 (fr) Modification de la biosynthese de la lignine
AU2003261795A1 (en) Method for detecting microbe or cell
WO2002016633A3 (fr) Procede d'identification de composes inhibant la proteolyse a mediation assuree par l'ubiquitine de ikb
AU2003238972A1 (en) Total organic carbon (toc) analyzer
AU2003217026A1 (en) Electrochemical multi-well plate
WO2005026392A3 (fr) Compositions, reactifs, et kits et procede de diagnostic, de controle et de traitement de l'obesite et/ou du diabete
WO2008021614A3 (fr) Réseaux de particules codées pour analyse d'analytes à haut rendement
WO2004070018A3 (fr) Modulation vegf-b et pdgf de cellules souches
WO2006026100A3 (fr) Methodes et kits de determination de la stabilite metabolique de composes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2453195

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2002320325

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2002749837

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 2002749837

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP