WO2003002735A3 - Methods, vectors, cell lines and kits for selecting nucleic acids having a desired feature - Google Patents

Methods, vectors, cell lines and kits for selecting nucleic acids having a desired feature Download PDF

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Publication number
WO2003002735A3
WO2003002735A3 PCT/CA2002/000997 CA0200997W WO03002735A3 WO 2003002735 A3 WO2003002735 A3 WO 2003002735A3 CA 0200997 W CA0200997 W CA 0200997W WO 03002735 A3 WO03002735 A3 WO 03002735A3
Authority
WO
WIPO (PCT)
Prior art keywords
desired feature
nucleic acids
kits
vectors
methods
Prior art date
Application number
PCT/CA2002/000997
Other languages
French (fr)
Other versions
WO2003002735A2 (en
Inventor
Christian Lanctot
Rock Gingras
Marie-Helene Gaumond
Original Assignee
Phenogene Therapeutiques Inc
Christian Lanctot
Rock Gingras
Marie-Helene Gaumond
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phenogene Therapeutiques Inc, Christian Lanctot, Rock Gingras, Marie-Helene Gaumond filed Critical Phenogene Therapeutiques Inc
Priority to EP02744992A priority Critical patent/EP1402020A2/en
Priority to CA002451957A priority patent/CA2451957A1/en
Publication of WO2003002735A2 publication Critical patent/WO2003002735A2/en
Publication of WO2003002735A3 publication Critical patent/WO2003002735A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/36011—Togaviridae
    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141—Use of virus, viral particle or viral elements as a vector
    • C12N2770/36143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/38—Vector systems having a special element relevant for transcription being a stuffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods, vectors, cell lines and kits for the screening and identification of nucleic acids are described. The invention is based on the use of a site-specific recombinase to excise, from an expression vector into which an exogenous nucleic acid having a desired feature has been inserted, a region of the vector which is excisable by site-specific recombination. Insertion into the vector of a nucleic acid having a desired feature, such as nucleic acids capable of changing the expression of cellular genes or the state of cellular metabolism or signaling pathways, triggers the synthesis and/or activity of a site specific recombinase, the action of the recombinase allowing an easy selection of the expression vector containing the exogenous nucleic acid having a desired feature.
PCT/CA2002/000997 2001-06-28 2002-06-28 Methods, vectors, cell lines and kits for selecting nucleic acids having a desired feature WO2003002735A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02744992A EP1402020A2 (en) 2001-06-28 2002-06-28 Methods, vectors, cell lines and kits for selecting nucleic acids having a desired feature
CA002451957A CA2451957A1 (en) 2001-06-28 2002-06-28 Methods, vectors, cell lines and kits for selecting nucleic acids having a desired feature

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30114901P 2001-06-28 2001-06-28
US60/301,149 2001-06-28

Publications (2)

Publication Number Publication Date
WO2003002735A2 WO2003002735A2 (en) 2003-01-09
WO2003002735A3 true WO2003002735A3 (en) 2003-05-30

Family

ID=23162156

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/000997 WO2003002735A2 (en) 2001-06-28 2002-06-28 Methods, vectors, cell lines and kits for selecting nucleic acids having a desired feature

Country Status (3)

Country Link
EP (1) EP1402020A2 (en)
CA (1) CA2451957A1 (en)
WO (1) WO2003002735A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116716350A (en) * 2023-01-18 2023-09-08 中国科学院深圳先进技术研究院 SINV vector for expressing IL-12 and application thereof in preparation of antitumor drugs

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0704534A2 (en) * 1994-09-19 1996-04-03 Sumitomo Pharmaceuticals Company, Limited Recombinant DNA viral vector for transfecting animal cells
WO1996040955A1 (en) * 1995-06-07 1996-12-19 Graham Frank L Adenovirus vectors for gene therapy
WO2002016572A2 (en) * 2000-08-18 2002-02-28 Phenogene Therapeutiques Inc. Nucleic acid molecules, methods and kits for selecting a nucleic acid having a desired feature

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0704534A2 (en) * 1994-09-19 1996-04-03 Sumitomo Pharmaceuticals Company, Limited Recombinant DNA viral vector for transfecting animal cells
WO1996040955A1 (en) * 1995-06-07 1996-12-19 Graham Frank L Adenovirus vectors for gene therapy
WO2002016572A2 (en) * 2000-08-18 2002-02-28 Phenogene Therapeutiques Inc. Nucleic acid molecules, methods and kits for selecting a nucleic acid having a desired feature

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LOGIE C ET AL: "LIGAND-REGULATED SITE-SPECIFIC RECOMBINATION", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 92, no. 13, 1 June 1995 (1995-06-01), pages 5940 - 5944, XP002025745, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
CA2451957A1 (en) 2003-01-09
EP1402020A2 (en) 2004-03-31
WO2003002735A2 (en) 2003-01-09

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