WO2002097100A3 - Vecteurs de clonage pour recombinaison homologue et procede les utilisant - Google Patents

Vecteurs de clonage pour recombinaison homologue et procede les utilisant Download PDF

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Publication number
WO2002097100A3
WO2002097100A3 PCT/FR2002/001782 FR0201782W WO02097100A3 WO 2002097100 A3 WO2002097100 A3 WO 2002097100A3 FR 0201782 W FR0201782 W FR 0201782W WO 02097100 A3 WO02097100 A3 WO 02097100A3
Authority
WO
WIPO (PCT)
Prior art keywords
homologous recombination
vectors
same
cloning vectors
concerns
Prior art date
Application number
PCT/FR2002/001782
Other languages
English (en)
Other versions
WO2002097100A2 (fr
Inventor
Christine Lapize-Gauthey
Alexandre Fraichard
Original Assignee
Genoway
Christine Lapize-Gauthey
Alexandre Fraichard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genoway, Christine Lapize-Gauthey, Alexandre Fraichard filed Critical Genoway
Priority to EP02740827A priority Critical patent/EP1392832A2/fr
Priority to CA002448983A priority patent/CA2448983A1/fr
Priority to US10/479,497 priority patent/US20040253732A1/en
Publication of WO2002097100A2 publication Critical patent/WO2002097100A2/fr
Publication of WO2002097100A3 publication Critical patent/WO2002097100A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cleaning And De-Greasing Of Metallic Materials By Chemical Methods (AREA)
  • Chemical Treatment Of Metals (AREA)

Abstract

La présente invention se rapporte à des nouveaux vecteurs utilisables à la fois pour créer des banques d'ADN génomiques et en tant que vecteurs pour la réalisation de réactions de recombinaison homologue dans des cellules hôtes, permettant notamment d'améliorer la sélection desdits événements de recombinaison homologue, et de diminuer le temps d'obtention du vecteur final pour effectuer la réaction de recombinaison homologue. L'invention a également pour objet un procédé mettant en oeuvre ces vecteurs.
PCT/FR2002/001782 2001-05-28 2002-05-28 Vecteurs de clonage pour recombinaison homologue et procede les utilisant WO2002097100A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02740827A EP1392832A2 (fr) 2001-05-28 2002-05-28 Vecteurs de clonage pour recombinaison homologue et procede les utilisant
CA002448983A CA2448983A1 (fr) 2001-05-28 2002-05-28 Vecteurs de clonage pour recombinaison homologue et procede les utilisant
US10/479,497 US20040253732A1 (en) 2001-05-28 2002-05-28 Cloning vectors for homologous recombination and method using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0106921A FR2825103B1 (fr) 2001-05-28 2001-05-28 Vecteurs de clonage pour recombinaison homologue et procede les utilisant
FR0106921 2001-05-28

Publications (2)

Publication Number Publication Date
WO2002097100A2 WO2002097100A2 (fr) 2002-12-05
WO2002097100A3 true WO2002097100A3 (fr) 2003-03-20

Family

ID=8863675

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/001782 WO2002097100A2 (fr) 2001-05-28 2002-05-28 Vecteurs de clonage pour recombinaison homologue et procede les utilisant

Country Status (5)

Country Link
US (2) US20040253732A1 (fr)
EP (1) EP1392832A2 (fr)
CA (1) CA2448983A1 (fr)
FR (1) FR2825103B1 (fr)
WO (1) WO2002097100A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2825507B1 (fr) * 2001-05-29 2003-12-26 Commissariat Energie Atomique Procede de decontamination ou de prevention de la contamination de surfaces par un mecanisme d'echange
EP1520020A2 (fr) * 2002-03-05 2005-04-06 Arrow Therapeutics Limited Transposon
US7785871B2 (en) * 2002-10-09 2010-08-31 Intrexon Corporation DNA cloning vector plasmids and methods for their use
US20080050808A1 (en) * 2002-10-09 2008-02-28 Reed Thomas D DNA modular cloning vector plasmids and methods for their use
WO2005116231A1 (fr) * 2004-05-18 2005-12-08 Intrexon Corporation Methodes pour assemblage de vecteurs dynamiques au moyen de plasmides vecteurs de clonage d'adn
US8153598B2 (en) * 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672510A (en) * 1990-01-19 1997-09-30 Genetic Therapy, Inc. Retroviral vectors
WO1998058067A1 (fr) * 1997-06-18 1998-12-23 Edge Biosystems, Inc. Vecteur et procede de preparation de banques d'adn
US5874259A (en) * 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector
WO2000026386A1 (fr) * 1998-11-04 2000-05-11 University Of Georgia Research Foundation, Inc. Systeme de vecteurs de transformation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL131125C (fr) * 1959-06-04
US3457107A (en) * 1965-07-20 1969-07-22 Diversey Corp Method and composition for chemically polishing metals
DE3143440A1 (de) * 1981-11-02 1983-05-19 Kernforschungszentrum Karlsruhe Gmbh, 7500 Karlsruhe Verfahren zur dekontamination von radioaktiv kontaminierten oberflaechen metallischer werkstoffe
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672510A (en) * 1990-01-19 1997-09-30 Genetic Therapy, Inc. Retroviral vectors
WO1998058067A1 (fr) * 1997-06-18 1998-12-23 Edge Biosystems, Inc. Vecteur et procede de preparation de banques d'adn
US5874259A (en) * 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector
WO2000026386A1 (fr) * 1998-11-04 2000-05-11 University Of Georgia Research Foundation, Inc. Systeme de vecteurs de transformation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MAHILLON J ET AL: "New ultrarare restriction site-carrying transposons for bacterial genomics", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 187, no. 2, 18 March 1997 (1997-03-18), pages 273 - 279, XP004093291, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
US20040253732A1 (en) 2004-12-16
FR2825103B1 (fr) 2003-09-19
WO2002097100A2 (fr) 2002-12-05
US20040168708A1 (en) 2004-09-02
EP1392832A2 (fr) 2004-03-03
FR2825103A1 (fr) 2002-11-29
CA2448983A1 (fr) 2002-12-05

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