WO2002094416A1 - Sterile laminar airflow device - Google Patents

Sterile laminar airflow device Download PDF

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Publication number
WO2002094416A1
WO2002094416A1 PCT/IN2001/000103 IN0100103W WO02094416A1 WO 2002094416 A1 WO2002094416 A1 WO 2002094416A1 IN 0100103 W IN0100103 W IN 0100103W WO 02094416 A1 WO02094416 A1 WO 02094416A1
Authority
WO
WIPO (PCT)
Prior art keywords
air
front panel
fan
sterile
removable
Prior art date
Application number
PCT/IN2001/000103
Other languages
French (fr)
Inventor
Rajesh Thakur
Anil Sood
Paramvir Singh Ahuja
Original Assignee
Council Of Scientific And Industrial Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council Of Scientific And Industrial Research filed Critical Council Of Scientific And Industrial Research
Priority to AU66306/01A priority Critical patent/AU782243B2/en
Priority to PCT/IN2001/000103 priority patent/WO2002094416A1/en
Priority to BR0107041-0A priority patent/BR0107041A/en
Priority to MYPI20021055A priority patent/MY129354A/en
Publication of WO2002094416A1 publication Critical patent/WO2002094416A1/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • B01D46/10—Particle separators, e.g. dust precipitators, using filter plates, sheets or pads having plane surfaces
    • B01D46/12—Particle separators, e.g. dust precipitators, using filter plates, sheets or pads having plane surfaces in multiple arrangements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
    • A61L9/18—Radiation
    • A61L9/20—Ultraviolet radiation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • B01D46/0027—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions
    • B01D46/0028—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions provided with antibacterial or antifungal means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • B01D46/52—Particle separators, e.g. dust precipitators, using filters embodying folded corrugated or wound sheet material
    • B01D46/521—Particle separators, e.g. dust precipitators, using filters embodying folded corrugated or wound sheet material using folded, pleated material

Definitions

  • the present invention relates to a compact, portable, lightweight, low power consuming, convenient, versatile and sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants. More particularly, the present invention relates to an apparatus for obtaining a working platform devoid of both biological and a-biological contaminants for tissue culture operations, watch and electronic device assembling, initiation of aseptic cultures of micro-organisms from field samples v situ'.
  • UN cabinets which are being used for this purpose, have the drawback of limited oxygen supply. This leads to restricted use. Also, it may endanger the safety of the user if the sprayed alcohol / rectified spirit inside the chamber does not dry up. Further, these are quite bulky and unsuitable for portability and do not offer flexibility in their usage e.g. in the electronic industry, as the materials need to be placed much before use.
  • the horizontal and vertical sterile laminar air flow systems which is also presently being used have the disadvantage of being heavy, bulky, and expensive in terms of cost and maintenance, because of which they are unsuitable for low end applications, where sterile environment is not required intensively, and more often a high infrastructure cost can not be afforded, such as micro-electronics works, cottage tissue culture practices, demonstration and teaching in schools; for field activities such as collection of biological samples like microbes and plants from far off places. Field applicability is further limited by the inevitable high power inputs required. At times, it becomes the limiting factor in dissemination of technologies at the grass root levels where these could be more suitable. It is well known that the hills, because of congenial environmental conditions are very rich in biodiversity that needs to be conserved.
  • the main object of the present invention is to provide a compact, lightweight, versatile and convenient sterile laminar airflow device.
  • Another object of the present invention is to provide a portable sterile laminar airflow device with a working platform with a sterile environment for carrying out aseptic operations.
  • Yet another object of the present invention is to provide a light weight, portable, compact, low power consuming sterile laminar air flow device that can be conveniently used in far flung remote areas for v situ' aseptic inoculation of biological samples such as plants and microbes for establishment of their cultures.
  • Still another object of the present invention is to provide a sterile laminar airflow device suitable for integration with other devices that may at times require sterile environment, such as the workbench of an enlarging device, thus increasing the versatility of applications.
  • One more object of the present invention is to provide a low cost sterile laminar airflow device for demonstration and teaching purpose.
  • the present invention relates to a compact, portable, lightweight, low power consuming, convenient, versatile and sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants.
  • the present invention provides a sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) divided into an upper & lower chambers; the upper chamber housing one or more pre-filtration members (B), a motor (T) mounted with fan (S), one or more HEPA filters (U), which are mounted on a gasket (N); and the lower chamber provided with a front panel (M) and housing a removable platform (X) and a perforated plane ( ⁇ ).
  • the body is made up of materials selected from the group comprising of solid wood, laminated wood, stainless steel or any other metal sheets and lightweight high-strength plastic.
  • the lightweight high-strength plastic is selected from the group comprising of Polycarbonate, fibre-glass and reinforced strengthened glass, plexi glass, polymethacrylate, polyester, polyethylene, polypropylene and polymethyl pentene (TPX).
  • the body has provisions for housing the dust filter, fan and HEPA filters.
  • the length to width ratio of the laminar sterile airflow device is in the range of 3 to 2.
  • the fan is of twin turbo types and it ensures continuous downward movement of sterile air.
  • the twin turbo fan is a heavy-duty axial fan
  • the front panel is preferably of sliding type.
  • the front panel has upward and downward restricted movement.
  • the front panel can be held at any desired position.
  • the platform is made up of materials selected from the group comprising of steel, wood, non-corrosive polyethylic material, non-corrosive polyplast material and non-corrosive metallic material.
  • the base of the lower chamber is perforated and with or without provision for removing the central part.
  • the central part of the perforated platform is made removable by a slit and groove mechanism, enabling integration of the device with other devices that may at times require sterile environment.
  • the perforated plate is made up of a non- corrosive material selected from the group comprising of metals, synthetic materials and wood.
  • the HEPA-filter opening towards the lower chamber is covered with a plastic grill.
  • the present invention further provides a compact, portable, lightweight, low power consuming, convenient and versatile sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) provided with mounts (O) and divided into upper & lower chambers; upper chamber provided with a removable panel (D) at the front for access to the internal components, housing a pre-filtration member (B) capable of retaining suspended particles in the air, a motor (T) with a twin turbo an (S) compartmentalized by a partition (R) into a suction channel for pushing the air entrapped in the pre-filtration member (B) unidirectionally through the air tight exhaust channel and the HEPA filter (U), which is mounted on a leak proof spongy rubber gasket (V), placed in the path of pressurized air, for further cleansing of the air of any biological or abiological air borne particulate contaminants so as to ensure a continuous flow of sterile air into the lower chamber and upon the removable solid platform (X
  • the device is provided with buckles on the sides for attachment of shoulder strap.
  • the device is provided with an illumination device.
  • the illumination device is selected from the group comprising of tube, bulb and halogen lamp.
  • the front and side panels may be transparent or opaque. In one another embodiment of the present invention, the front and side panels are made up of unbreakable UN stabilized material.
  • the front panel should preferably be of sliding type. In another embodiment of the present invention, the front panel has upward and downward restricted movement.
  • the front panel can be held at any desired position.
  • the front panel is held at different positions by a screw and spring mechanism.
  • the device is provided with removable legs to have clearance from the table.
  • the device is provided with an UN source.
  • the UN source is UN tube.
  • the UN tube is fixed below the upper chamber.
  • device for measuring air pressure is installed in the upper chamber.
  • the air pressure-measuring device monitors and regulates the inside pressure, and hence the airflow through the HEPA filters.
  • the switches control the air flow and light intensities.
  • control switch is selected from the group comprising of O ⁇ -OFF click type switch, sliding type switch, and dimmer type switch with rotary motion.
  • the device is provided with a platform with perforations.
  • the platform is made up of materials selected from the group comprising of steel, wood, non-corrosive polyethylic material, non-corrosive polyplast material and non-corrosive metallic material.
  • the central part of the perforated platform is made removable by a slit and groove mechanism, enabling integration of the device with other devices that may at times require sterile environment.
  • the base of the lower chamber is perforated and with or without provision for removing the central part.
  • the pre-filter is covered with a perforated plate.
  • the perforated plate is made up of a non-corrosive material selected from the group comprising metal, synthetic material and wood.
  • the HEPA-filter opening towards the lower chamber has been covered with a plastic grill.
  • the entire device can be carried in a lightweight box.
  • the device can be operated on both ordinary power supply and battery.
  • the device can be used as workbench of an enlarging device.
  • Figure I represents the perspective view of the present device called 'STERIFLOW' embodying the present invention.
  • Figure II represents the perspective view of the vertical section taken on the line 2-2 of FIG. I.
  • Figure III represents the front view of the vertical section taken on the line 2-2 of FIG I, indicating the direction of the airflow streamlines that would affect particulate trajectories in the STERIFLOW.
  • A) Handle Swing type handle, centrally located at the top of the body such that it does not come into the way of the air inlet.
  • B) Pre-filter A fibrous washable filter sheet with large pore size and folded setup for high surface area to filter restrict the intake of suspended dust particles.
  • Upper Front Panel A wooden panel 6 mm in thickness, fixed in front position of the upper chamber with screws, and allows access to the internal components for servicing.
  • E) Regulator An electronic fan regulator with maximum capacity of 600 watts operatable at 240N AC 50 Hz electricity supply, to regulate the speed of the motor, turbo fan and hence, the speed of the sterile air flow.
  • H) Indicator Switches Two indicator switches of 6 Amp capacity, operable at 250 V AC, placed on the side of the body (C) to facilitate the switching on and off the motor (T) and the germicidal UN lamp (J).
  • J) Germicidal UV Lamp A s Sankyo Denki', Japan brand 21 cm long 6 Watt shortwave UN tube placed at the rear side, below the upper chamber to evenly illuminate the lower chamber and any objects kept on the working platform ( ⁇ ).
  • Lower Front Panel 4 mm thick rectangular UN stabilized transparent polycarbonate plate of dimensions 32 x 27 (cm): W x H, the left and right margins of which are notched (G) at regular intervals, which when obstructed by the ball, spring & screw system (I) provide a mechanism of holding it at desired heights.
  • the lower edge is partially trimmed in an oblique manner to avoid obstruction by the ball, spring screw system (I) during the initial insertion into the body (C).
  • the shape and size are such that upon complete closure it does not obstruct with the removal of the upper front panel (D). It has a handle (F) fixed to it as a site for gripping with hand, to facilitate its vertical movement.
  • Perforated Plate A removable stainless steel perforated plate, with such shape that it fits well into the working platform (X). The perforations facilitate partial vertically downward flow of the sterile air.
  • the perforated plate can be replaced by a solid plate made of laminated wood, glass, stainless steel or any other non-corrosive material as desired but not limited to them.
  • Rubberized legs 2 cm tall rubberized legs that keep the working platform (X) raised above the surface, thus allowing air passage from beneath the working platform. It also dampens the vibrations in the body due to activity of the motor (T), resulting in a smoother operation.
  • a pair of turbo fans are provided to minimize any recirculation of air due to leakage from the exhaust channel to the suction channel, and to maximize the unidirectional flow of air so as to build up higher pressures required to drive the air through the HEPA filter.
  • the fan motor has the following specifications: i. Power : 200 Watts, (0.003 units/minute) ii. Operation voltage : 230V AC, 50 Hz, iii. Current : 0.95 Amp, iv. Suction strength : 650 mm water column, v. Speed : 15500 -16000 rpm U) HEPA filter: A setup of high surface area folded filter membrane with a cut off pore size of 0.2 microns located at the end of the exhaust channel in the upper chamber, upon passing through which the air pressurized by the motor and twin turbo fan generate a vertically downwards stream of sterile air into the lower chamber hence, creating a sterile environment above the working platform (X).
  • Spongy rubber strip A strip of spongy rubber is sandwiched along the circumference of the HEPA module, between the HEPA module and the lower wall of the upper chamber of the body (C) to which the HEPA module is fixed, to avoid any air leakage from the upper chamber into the lower chamber except through the HEPA filter.
  • the working of the sterile airflow device STERIFLOW for obtaining a continuous flow of clean air at the perforated bench area is described below: The STERIFLOW is switched on so that the air is sucked into the upper chamber because of the fan and is filtered through the pre-filter which retains the dust particles.
  • EXAMPLE - 1 the pre-sterilized petriplates were first kept in the working platform along the arms of a cross stretching across the corners of the working platform for 10 minutes with UN light on and then still hot sterilized liquid MS medium containing sucrose (30% w/v), vitamins and agar for solidification, was poured into them and allowed to solidify for 15 minutes. The petriplates were then sealed with parafilm (M/s Sigma, USA) and incubated to check for any contamination for 3 weeks. No contamination in these petriplates confirmed the proper working of the present invention.
  • EXAMPLE - 2 The STERIFLOW as shown in Fig I was used for raising the aseptic cultures of the nodal explants of a bamboo (Dendrocalamus hamiltonii). The nodal explants after surface sterilization with 0.04% solution of HgCl 2 (w/v) containing a drop of liquid detergent were transferred on to the modified Murashige and Skoog (1962) medium containing a cytokinin, benzyl adenine and an auxin, 2,4-dichlorophenoxy acetic acid (2,4-D; 1.0 mg 1 each). There was no contamination because of any bacteria or fungi even after three weeks of incubation period, affirming that the STERIFLOW is working in the desirable manner.
  • the present invention is a low power consuming, portable, versatile, convenient device capable of providing continuous flow of clean air on a working platform making it suitable for all those industries requiring clean areas at the working benches.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Filtering Of Dispersed Particles In Gases (AREA)
  • Accommodation For Nursing Or Treatment Tables (AREA)

Abstract

A compact, portable, lightweight, low power consuming, convenient, versatile and sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) divided into an upper and lower chambers; the upper chamber housing one or more pre-filtration members (B), a motor (T) mounted with fan (S), and one or more filters (U) located below the motor; and the lower chamber provided with a front panel (M), a removable platform (X) located at the lower portion of the chamber and a perforated plane (N) placed on the removable platform.

Description

STERILE LAMINAR AIRFLOW DEVICE Field of Invention
The present invention relates to a compact, portable, lightweight, low power consuming, convenient, versatile and sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants. More particularly, the present invention relates to an apparatus for obtaining a working platform devoid of both biological and a-biological contaminants for tissue culture operations, watch and electronic device assembling, initiation of aseptic cultures of micro-organisms from field samples v situ'. Background of the Invention
In tissue culture and industries like electronic industry, watch industry etc., the requirements for clean area are near absolute and even a small leakage or mishandling can lead to disastrous results in the production line. Such is the essentiality of the clean area or clean benches that substantial amount of money goes into the erection of these structures and the maintenance of the same. Maintaining these structures is also expensive leading to an escalation in the ultimate cost of production.
UN cabinets, which are being used for this purpose, have the drawback of limited oxygen supply. This leads to restricted use. Also, it may endanger the safety of the user if the sprayed alcohol / rectified spirit inside the chamber does not dry up. Further, these are quite bulky and unsuitable for portability and do not offer flexibility in their usage e.g. in the electronic industry, as the materials need to be placed much before use. The horizontal and vertical sterile laminar air flow systems which is also presently being used have the disadvantage of being heavy, bulky, and expensive in terms of cost and maintenance, because of which they are unsuitable for low end applications, where sterile environment is not required intensively, and more often a high infrastructure cost can not be afforded, such as micro-electronics works, cottage tissue culture practices, demonstration and teaching in schools; for field activities such as collection of biological samples like microbes and plants from far off places. Field applicability is further limited by the inevitable high power inputs required. At times, it becomes the limiting factor in dissemination of technologies at the grass root levels where these could be more suitable. It is well known that the hills, because of congenial environmental conditions are very rich in biodiversity that needs to be conserved. Taking plant or microbe samples and bringing them to the base laboratory are always accompanied with dangers of cross contamination leading to spoilage of precious materials and time as well. Therefore, the suitability of a low power consuming, portable, sterile laminar airflow device is highly desirable.
Objects of the Invention
The main object of the present invention is to provide a compact, lightweight, versatile and convenient sterile laminar airflow device. Another object of the present invention is to provide a portable sterile laminar airflow device with a working platform with a sterile environment for carrying out aseptic operations.
Yet another object of the present invention is to provide a light weight, portable, compact, low power consuming sterile laminar air flow device that can be conveniently used in far flung remote areas for v situ' aseptic inoculation of biological samples such as plants and microbes for establishment of their cultures.
Still another object of the present invention is to provide a sterile laminar airflow device suitable for integration with other devices that may at times require sterile environment, such as the workbench of an enlarging device, thus increasing the versatility of applications.
One more object of the present invention is to provide a low cost sterile laminar airflow device for demonstration and teaching purpose.
One another object of the present invention is to provide a low cost sterile laminar airflow device for promotion of plant tissue culture as a cottage industry. Another object of the present invention is to provide a portable, versatile, convenient sterile laminar airflow device, that is small in size, requires less space and can therefore, be used for desktop sterile air applications
Summary of the Invention
The present invention relates to a compact, portable, lightweight, low power consuming, convenient, versatile and sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants.
Detailed Description of the invention
Accordingly, the present invention provides a sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) divided into an upper & lower chambers; the upper chamber housing one or more pre-filtration members (B), a motor (T) mounted with fan (S), one or more HEPA filters (U), which are mounted on a gasket (N); and the lower chamber provided with a front panel (M) and housing a removable platform (X) and a perforated plane (Ν). In an embodiment of the present invention, the body is made up of materials selected from the group comprising of solid wood, laminated wood, stainless steel or any other metal sheets and lightweight high-strength plastic.
In another embodiment of the present invention, the lightweight high-strength plastic is selected from the group comprising of Polycarbonate, fibre-glass and reinforced strengthened glass, plexi glass, polymethacrylate, polyester, polyethylene, polypropylene and polymethyl pentene (TPX).
In still another embodiment of the present invention, the body has provisions for housing the dust filter, fan and HEPA filters. In yet another embodiment of the present invention, the length to width ratio of the laminar sterile airflow device is in the range of 3 to 2.
In one more embodiment of the present invention, the fan is of twin turbo types and it ensures continuous downward movement of sterile air.
In one another embodiment of the present invention, the twin turbo fan is a heavy-duty axial fan
In an embodiment of the present invention, the front panel is preferably of sliding type.
In another embodiment of the present invention, the front panel has upward and downward restricted movement.
In still another embodiment of the present invention, the front panel can be held at any desired position.
In yet another embodiment of the present invention, the platform is made up of materials selected from the group comprising of steel, wood, non-corrosive polyethylic material, non-corrosive polyplast material and non-corrosive metallic material.
In one more embodiment of the present invention, the base of the lower chamber is perforated and with or without provision for removing the central part.
In one another embodiment of the present invention, the central part of the perforated platform is made removable by a slit and groove mechanism, enabling integration of the device with other devices that may at times require sterile environment.
In an embodiment of the present invention, the perforated plate is made up of a non- corrosive material selected from the group comprising of metals, synthetic materials and wood.
In another embodiment of the present invention, the HEPA-filter opening towards the lower chamber is covered with a plastic grill.
The present invention further provides a compact, portable, lightweight, low power consuming, convenient and versatile sterile laminar airflow device, useful in obtaining workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) provided with mounts (O) and divided into upper & lower chambers; upper chamber provided with a removable panel (D) at the front for access to the internal components, housing a pre-filtration member (B) capable of retaining suspended particles in the air, a motor (T) with a twin turbo an (S) compartmentalized by a partition (R) into a suction channel for pushing the air entrapped in the pre-filtration member (B) unidirectionally through the air tight exhaust channel and the HEPA filter (U), which is mounted on a leak proof spongy rubber gasket (V), placed in the path of pressurized air, for further cleansing of the air of any biological or abiological air borne particulate contaminants so as to ensure a continuous flow of sterile air into the lower chamber and upon the removable solid platform (X) / perforated plane (N); switches with indicator lights (H) on the side for controlling the operations; a speed controller (E) for regulating fan or motor speed; a large centrally located handle (A) at the top for ease of carrying with single hand; buckles for attaching standard shoulder straps (W); a lower chamber with an assembly for a UN tube (J) with germicidal properties; openable panels on sides (L, P) with hinge joints ( ) along the rear vertical axis, and a lower front panel (M) with such height that at completely closed position it does not obstruct the removal of front panel of the upper chamber; openable by sliding movement in vertical plane along the vertical slot (Q) in the body, through a horizontal slot (Y) at the front above the lower chamber, facilitated by an appropriately placed handle (F) with provision for holding the whole panel at different heights, with the help of notches (G) along the side edges, which are obstructed by the ball, spring and screw system (I) fixed to the body wall; fibrous mat packing provided in the horizontal slot Υ to prevent free movement of air and dust particles. In an embodiment of the present invention, the device is provided with a handle as a carrying device.
In another embodiment of the present invention, the device is provided with buckles on the sides for attachment of shoulder strap. In still another embodiment of the present invention, the device is provided with an illumination device.
In yet another embodiment of the present invention, the illumination device is selected from the group comprising of tube, bulb and halogen lamp.
In one more embodiment of the present invention, the front and side panels may be transparent or opaque. In one another embodiment of the present invention, the front and side panels are made up of unbreakable UN stabilized material.
In an embodiment of the present invention, the front panel should preferably be of sliding type. In another embodiment of the present invention, the front panel has upward and downward restricted movement.
In still another embodiment of the present invention, the front panel can be held at any desired position.
In yet another embodiment of the present invention, the front panel is held at different positions by a screw and spring mechanism.
In one more embodiment of the present invention, the device is provided with removable legs to have clearance from the table.
In one another embodiment of the present invention, the device is provided with an UN source. In an embodiment of the present invention, the UN source is UN tube.
In another embodiment of the present invention, the UN tube is fixed below the upper chamber.
In still another embodiment of the present invention, device for measuring air pressure is installed in the upper chamber. In yet another embodiment of the present invention, the air pressure-measuring device monitors and regulates the inside pressure, and hence the airflow through the HEPA filters.
In one more embodiment of the present invention, the switches control the air flow and light intensities.
In one another embodiment of the present invention, the control switch is selected from the group comprising of OΝ-OFF click type switch, sliding type switch, and dimmer type switch with rotary motion.
In an embodiment of the present invention, the device is provided with a platform with perforations.
In another embodiment of the present invention, the platform is made up of materials selected from the group comprising of steel, wood, non-corrosive polyethylic material, non-corrosive polyplast material and non-corrosive metallic material.
In still another embodiment of the present invention, the central part of the perforated platform is made removable by a slit and groove mechanism, enabling integration of the device with other devices that may at times require sterile environment. In yet another embodiment of the present invention, the base of the lower chamber is perforated and with or without provision for removing the central part.
In one more embodiment of the present invention, the pre-filter is covered with a perforated plate. In one another embodiment of the present invention, the perforated plate is made up of a non-corrosive material selected from the group comprising metal, synthetic material and wood.
In an embodiment of the present invention, the HEPA-filter opening towards the lower chamber has been covered with a plastic grill. In another embodiment of the present invention, the entire device can be carried in a lightweight box.
In still another embodiment of the present invention, the device can be operated on both ordinary power supply and battery.
In yet another embodiment of the present invention, the device can be used as workbench of an enlarging device.
Brief Description of the accompanying Drawings
In the drawings accompanying the specification, Figure I represents the perspective view of the present device called 'STERIFLOW' embodying the present invention.
In the drawings accompanying the specification, Figure II represents the perspective view of the vertical section taken on the line 2-2 of FIG. I.
In the drawings accompanying the specification, Figure III represents the front view of the vertical section taken on the line 2-2 of FIG I, indicating the direction of the airflow streamlines that would affect particulate trajectories in the STERIFLOW.
Details of the individual parts of the device called 'STERIFLOW' are given below by way of illustration, and this should not be construed to limit the invention in any manner:
A) Handle: Swing type handle, centrally located at the top of the body such that it does not come into the way of the air inlet.
B) Pre-filter: A fibrous washable filter sheet with large pore size and folded setup for high surface area to filter restrict the intake of suspended dust particles. C) Body: A wooden cabinet 35 x 25 x 50 (cm): W x D x H and wall thickness 2cm having provided with rubberised mounts (O) of 2cm height; and divided into upper & lower chambers; upper chamber of internal dimensions 31 x 21 x 21 (cm) : W x D x H, with a removable panel (D) at the front for access to the internal components, housing a filtration member (B) capable of retaining suspended particles in the air, a motor (T) with a twin turbo fan (S) compartmentalized by a partition (R) into a suction channel for pushing the air entrapped in the pre-filtration member (B) unidirectionally through the air tight exhaust channel and the HEPA filter (U), which is mounted on a leak proof spongy rubber gasket (V), placed in the path of pressurized air, for further cleansing of the air of any biological or abiological air borne particulate contaminants so as to ensure a continuous flow of sterile air into the lower chamber and on the removable solid platform (X) / perforated plane (N); a lower chamber of internal dimensions 35 x 21 x 23 (cm) : W x D x H, with an assembly for UN tube (J) with germicidal properties, a solid platform (X) and a perforated plane (Ν); switches with indicator lights (H) on the side for controlling the operations; a speed controller (E) for regulating fan or motor speed; a large centrally located handle (A) at the top for ease of carrying with a single hand, g) buckles for attaching standard shoulder straps (W); openable panels on sides (L, P) with hinge joints (K) along the rear vertical axis, and a front panel (M) with height = 27 cm so that at closed position it does not obstruct the removal of front panel of the upper chamber; openable by sliding movement in vertical plane along the vertical slot (Q) in the body, through a horizontal slot (Y)at the front above the lower chamber, facilitated by an appropriately placed handle (F) with provision for holding the whole panel at different heights, with the help of notches along the side edges (G), which are obstructed by the ball, spring & screw system (I) fixed to the body wall; fibrous mat packing fixed to the body, between the front panel of the lower chamber and the boundaries of the horizontal slot above the lower chamber for the vertically slidable front panel to prevent free movement of air.
D) Upper Front Panel: A wooden panel 6 mm in thickness, fixed in front position of the upper chamber with screws, and allows access to the internal components for servicing.
E) Regulator: An electronic fan regulator with maximum capacity of 600 watts operatable at 240N AC 50 Hz electricity supply, to regulate the speed of the motor, turbo fan and hence, the speed of the sterile air flow. H) Indicator Switches: Two indicator switches of 6 Amp capacity, operable at 250 V AC, placed on the side of the body (C) to facilitate the switching on and off the motor (T) and the germicidal UN lamp (J). J) Germicidal UV Lamp: A sSankyo Denki', Japan brand 21 cm long 6 Watt shortwave UN tube placed at the rear side, below the upper chamber to evenly illuminate the lower chamber and any objects kept on the working platform (Ν).
M) Lower Front Panel: 4 mm thick rectangular UN stabilized transparent polycarbonate plate of dimensions 32 x 27 (cm): W x H, the left and right margins of which are notched (G) at regular intervals, which when obstructed by the ball, spring & screw system (I) provide a mechanism of holding it at desired heights. The lower edge is partially trimmed in an oblique manner to avoid obstruction by the ball, spring screw system (I) during the initial insertion into the body (C). The shape and size are such that upon complete closure it does not obstruct with the removal of the upper front panel (D). It has a handle (F) fixed to it as a site for gripping with hand, to facilitate its vertical movement.
N) Perforated Plate: A removable stainless steel perforated plate, with such shape that it fits well into the working platform (X). The perforations facilitate partial vertically downward flow of the sterile air. The perforated plate can be replaced by a solid plate made of laminated wood, glass, stainless steel or any other non-corrosive material as desired but not limited to them.
O) Rubberized legs: 2 cm tall rubberized legs that keep the working platform (X) raised above the surface, thus allowing air passage from beneath the working platform. It also dampens the vibrations in the body due to activity of the motor (T), resulting in a smoother operation.
S) Twin turbo fan: A pair of turbo fans are provided to minimize any recirculation of air due to leakage from the exhaust channel to the suction channel, and to maximize the unidirectional flow of air so as to build up higher pressures required to drive the air through the HEPA filter.
T) Motor: The fan motor has the following specifications: i. Power : 200 Watts, (0.003 units/minute) ii. Operation voltage : 230V AC, 50 Hz, iii. Current : 0.95 Amp, iv. Suction strength : 650 mm water column, v. Speed : 15500 -16000 rpm U) HEPA filter: A setup of high surface area folded filter membrane with a cut off pore size of 0.2 microns located at the end of the exhaust channel in the upper chamber, upon passing through which the air pressurized by the motor and twin turbo fan generate a vertically downwards stream of sterile air into the lower chamber hence, creating a sterile environment above the working platform (X). V) Spongy rubber strip: A strip of spongy rubber is sandwiched along the circumference of the HEPA module, between the HEPA module and the lower wall of the upper chamber of the body (C) to which the HEPA module is fixed, to avoid any air leakage from the upper chamber into the lower chamber except through the HEPA filter. The working of the sterile airflow device STERIFLOW for obtaining a continuous flow of clean air at the perforated bench area is described below: The STERIFLOW is switched on so that the air is sucked into the upper chamber because of the fan and is filtered through the pre-filter which retains the dust particles. Soon the pressure builds up within the upper chamber and the air is then forced through the HEPA filter which takes care of any remaining suspended particles both biological and abiological so that clean air flows in a continuous stream across the filter and onto the working area which also gets cleared of any suspended particles after 10-12 minutes and can be used for sterile operations. In case of biological operations, the use of UN light for atleast 10 minutes is recommended to kill already existing contaminants. A spirit lamp or a gas burner to flame sterilize the mouths of the culture vessels before carrying out operations involving live biological material can also be used. The following examples are given by way of illustration of the utility of the present invention (STERIFLOW) for raising aseptic cultures and should not be construed to limit the scope of the present invention.
EXAMPLE - 1 In the example, the pre-sterilized petriplates were first kept in the working platform along the arms of a cross stretching across the corners of the working platform for 10 minutes with UN light on and then still hot sterilized liquid MS medium containing sucrose (30% w/v), vitamins and agar for solidification, was poured into them and allowed to solidify for 15 minutes. The petriplates were then sealed with parafilm (M/s Sigma, USA) and incubated to check for any contamination for 3 weeks. No contamination in these petriplates confirmed the proper working of the present invention.
EXAMPLE - 2 The STERIFLOW as shown in Fig I was used for raising the aseptic cultures of the nodal explants of a bamboo (Dendrocalamus hamiltonii). The nodal explants after surface sterilization with 0.04% solution of HgCl2 (w/v) containing a drop of liquid detergent were transferred on to the modified Murashige and Skoog (1962) medium containing a cytokinin, benzyl adenine and an auxin, 2,4-dichlorophenoxy acetic acid (2,4-D; 1.0 mg 1 each). There was no contamination because of any bacteria or fungi even after three weeks of incubation period, affirming that the STERIFLOW is working in the desirable manner.
Main advantages of the present invention are:
1. The present invention is a low power consuming, portable, versatile, convenient device capable of providing continuous flow of clean air on a working platform making it suitable for all those industries requiring clean areas at the working benches.
2. It provides a light weight, portable, compact, low energy consuming sterile laminar air flow cabinet that can be conveniently used in far flung remote areas for "in situ' aseptic inoculation of biological samples such as plants and microbes for establishment of cultures. 3. Provision of a large handle for carrying and buckles for attaching flexible shoulder belts, in addition to the small compact size and light weight, make the device truly portable.
4. It is a low cost sterile laminar air flow cabinet for promotion of plant tissue culture as a cottage industry, demonstration and education in schools and colleges, and for industries with low end requirement such as watch industry and micro-electronics industry. 5. It being small in size, requires less space and can therefore, be used for desktop sterile air applications. 6. The working platform being partially removable, the device can be integrated with other devices that may at times require sterile environment, such as the workbench of an image enlarging device, thus increasing the versatility of applications.
7. Provision for opening or closing the front and the side panels, make manipulation inside the sterile zone within the lower chamber possible from any direction as per convenience.
8. Maintenance is negligible as except for the HEPA filters, the rest of the items are available easily in the market.

Claims

Claims
1. A sterile laminar airflow device useful in obtaining workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) divided into an upper and lower chambers; the upper chamber housing one or more pre- filtration members (B), a motor (T) mounted with fan (S), and one or more filters
(U) located below the motor; and the lower chamber provided with a front panel (M), a removable platform (X) located at the lower portion of the chamber and a perforated plane (N) placed on the removable platform.
2. A device as claimed in claim 1, further comprising removable legs or mounts (O) for providing clearance from any surface on which the device is kept.
3. A device as claimed in claim 1, wherein the motor (T) with the fan (S) is compartmentalized by a partition (R) into a suction channel through the pre-filter and the filter (U) for pushing the entrapped air unidirectionally into the lower chamber.
4. A device as claimed in claim 1, further comprising one or more switches (H) on the sides of the device for controlling the operations; a speed controller (E) for regulating fan or motor speed; an illuminating device; and a UN source with germicidal properties being provided in the lower chamber.
5. A device as claimed in claiml, further comprising openable panels on sides (L, P) with hinge joints (K) along the rear vertical axis, a lower front panel (M) provided with a handle (F) of such height that at completely closed position it does not obstruct the removal of front panel of the upper chamber; a vertical slot (Q) and a horizontal slot (Y) for moving the front panel, notches (G) and spring and screw mechanism (I) along the side edges to hold the front panel at different heights.
6. A device as claimed in claim 1, wherein a large centrally located handle (A) is provided at the top of the device and buckles for attaching shoulder straps.
7. A device as claimed in claim 4, wherein the UV source is a UN tube.
8. A device as claimed in claim 4, wherein the illuminating device is selected from the group comprising of tube, bulb and halogen lamp.
9. A device as claimed in claim 1, wherein the body is made up of materials selected from the group comprising of solid wood, laminated wood, stainless steel or any other metal sheets and lightweight high-strength plastics.
10. A device as claimed in claim 7, wherein the lightweight high-strength plastic is selected from the group comprising of Polycarbonate, fibre-glass reinforced strengthened glass, plexi glass, polymethacrylate, polyester, polyethylene, polypropylene and polymethyl pentene (TPX).
11. A device as claimed in claim 1, wherein the length to width ratio of the laminar airflow device is in the range of 3: 2.
12. A device as claimed in claim 1, wherein the fan is a twin turbo fan which ensures continuous downward movement of sterile air.
13. A device as claimed in claim 1, wherein the turbo fan is a heavy-duty axial fan
14. A compact, portable, lightweight, low power consuming, convenient and versatile sterile laminar airflow device useful in obtaining workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) provided with mounts (O) and divided into upper & lower chambers; upper chamber provided with a removable panel (D) at the front for access to the internal components, housing a pre-filtration member (B) capable of retaining suspended particles in the air, a motor (T) with a twin turbo fan (S) compartmentalized by a partition (R) into a suction channel for pushing the air entrapped in the pre-filtration member (B) unidirectionally through the air tight exhaust channel and the HEPA filter (U) , which is mounted on a leak proof spongy rubber gasket (N), placed in the path of pressurized air, for further cleansing of the air of any biological or abiological air borne particulate contaminants so as to ensure a continuous flow of sterile air into the lower chamber and upon the removable solid platform (X) / perforated plane (Ν); switches with indicator lights (H) on the side for controlling the operations; a speed controller (E) for regulating fan or motor speed; a large centrally located handle (A) at the top for ease of carrying with single hand; buckles for attaching standard shoulder straps (W); a lower chamber with an assembly for a UN source (J) with germicidal properties; openable panels on sides (L, P) with hinge joints (K) along the rear vertical axis, and a lower front panel (M) with such height that at completely closed position it does not obstruct the removal of front panel of the upper chamber; openable by sliding movement in vertical plane along the vertical slot (Q) in the body, through a horizontal slot (Y) at the front above the lower chamber, facilitated by an appropriately placed handle (F) with provision for holding the whole panel at different heights, with the help of notches (G) along the side edges, which are obstructed by the ball, spring and screw system (I) fixed to the body wall; and fibrous mat packing provided in the horizontal slot Υ to prevent free movement of air and dust particles.
15. A device as claimed in claim 14, wherein the device is provided with a handle as a carrying device.
16. A device as claimed in claim 14, wherein the device is provided with buckles on the sides for attachment of shoulder strap.
17. A device as claimed in claim 14, wherein the device is provided with an illumination device.
18. A device as claimed in claim 17, wherein the illumination device is selected from the group comprising of tube, bulb and halogen lamp.
19. A device as claimed in claim 14, wherein the front and side panels may be transparent or opaque.
20. A device as claimed in claim 19, wherein the front and side panels are made up of unbreakable UN stabilized material.
21. A device as claimed in claim 19, wherein the front panel may be of sliding type.
22. A device as claimed in claim 19, wherein the front panel has upward and downward movement.
23. A device as claimed in claim 19, wherein the front panel can be held at any desired position.
24. A device as claimed in claim 19, wherein a screw and spring mechanism holds the front panel in any desired position.
25. A device as claimed in claim 14, wherein the device is provided with removable legs for clearance.
26. A device as claimed in claim 14, wherein the UN source is UN tube.
27. A device as claimed in claim 26, wherein the UN tube is fixed below the upper chamber.
28. A device as claimed in claim 14, wherein an air pressure measuring device is installed in the upper chamber for monitoring and regulating pressure inside the device, and hence the air flowing through the HEPA filter.
29. A device as claimed in claim 14, wherein the switches control the air flow and light intensities.
30. A device as claimed in claim 29, wherein the control switch is selected from the group comprising of OΝ-OFF click type switch, sliding type switch, and dimmer type switch with rotary motion.
31. A device as claimed in claim 14, wherein the device is provided with a platform with perforations.
32. A device as claimed in claim 31, wherein the platform is made up of materials selected from the group comprising of steel, wood, non-corrosive polyethylic material, non-corrosive polyplast material and non-corrosive metallic material.
33. A device as claimed in claim 31, wherein the central part of the perforated platform is made removable by a slit and groove mechanism, enabling integration of the device with other devices that may at times require sterile environment.
34. A device as claimed in claim 14, wherein the base of the lower chamber is perforated and with or without provision for removing the central part.
35. A device as claimed in claim 14, wherein the pre-filter is covered with a perforated plate.
36. A device as claimed in claim 35, wherein the perforated plate is made up of a non- corrosive materials selected from the group comprising of metals, synthetic materials and wood.
37. A device as claimed in claim 14, wherein the HEPA-filter is covered with a plastic grill.
38. A device as claimed in claim 14, wherein the entire device may be carried in a light weight box.
39. A device as claimed in claim 14, wherein the device can be operated on both ordinary power supply and battery.
40. A device as claimed in claim 14, wherein the device can be used as workbench of an enlarging device.
PCT/IN2001/000103 2001-05-21 2001-05-21 Sterile laminar airflow device WO2002094416A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU66306/01A AU782243B2 (en) 2001-05-21 2001-05-21 Sterile laminar airflow device
PCT/IN2001/000103 WO2002094416A1 (en) 2001-05-21 2001-05-21 Sterile laminar airflow device
BR0107041-0A BR0107041A (en) 2001-05-21 2001-05-21 Sterilized Laminar Airflow Device
MYPI20021055A MY129354A (en) 2001-05-21 2002-03-25 Sterile laminar airflow device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IN2001/000103 WO2002094416A1 (en) 2001-05-21 2001-05-21 Sterile laminar airflow device

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FR2935603A1 (en) * 2008-09-08 2010-03-12 Edra Medical Aseptic storage cabinet for e.g. maintaining endoscopes, has insufflation unit insufflating ambient air through upper wall so as to produce laminar stream of air towards opening, where opening is situated in lower zone of enclosure
WO2011054467A1 (en) * 2009-11-05 2011-05-12 Rheinmetall Landsysteme Gmbh Safety workbench
ITMI20101050A1 (en) * 2010-06-10 2011-12-11 Tecniplast Spa CHANGE CABIN LAMINAR AIR FLOW CAGE MADE OF PLASTIC MATERIAL
WO2014024211A3 (en) * 2012-08-06 2014-04-10 Council Of Scientific & Industrial Research Bioreactor vessel for large scale growing of plants under aseptic conditions
CN105709523A (en) * 2016-01-29 2016-06-29 太仓东能环保设备有限公司 Sterilization air purifier
EP3069800A1 (en) * 2015-03-17 2016-09-21 Kojair Tech Oy Biosafety cabinet and method of sterilising an object
CN106439633A (en) * 2016-10-28 2017-02-22 湖南正海现代实验室设备有限公司 OLED (Organic Light Emitting Diode) illuminating lamp for ventilation cabinet of laboratory
US9872448B2 (en) 2012-08-06 2018-01-23 Council Of Scientific & Industrial Research Bioreactor vessel for large scale growing of plants under aseptic condition
WO2018027303A1 (en) * 2016-08-09 2018-02-15 Saed Technologies Ltd. Method and apparatus for regeneration, acclimatization, and conditioning of plant propagules
CN108050589A (en) * 2017-11-24 2018-05-18 苏州赛易特环保科技有限公司 A kind of portable air cleaning unit
CN110252040A (en) * 2019-07-30 2019-09-20 淮北雷德机电科技有限公司 A kind of environmental protection pneumatic filter
CN111687163A (en) * 2019-03-14 2020-09-22 安徽一洋实验设备有限公司 Environment-friendly self-loopa ventilation medicine cabinet
CN113274223A (en) * 2021-05-21 2021-08-20 吉林大学 Sterile laminar flow chamber for aplastic anemia patients
RU209286U1 (en) * 2021-11-16 2022-03-14 Людмила Владимировна Матвеенко Air recirculator ultraviolet bactericidal
GR1010197B (en) * 2021-05-21 2022-03-17 Ευσταθιος Ελευθεριου Τσατσαλακης Wheeled device for air sterilization and surface disinfection

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Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2935603A1 (en) * 2008-09-08 2010-03-12 Edra Medical Aseptic storage cabinet for e.g. maintaining endoscopes, has insufflation unit insufflating ambient air through upper wall so as to produce laminar stream of air towards opening, where opening is situated in lower zone of enclosure
WO2011054467A1 (en) * 2009-11-05 2011-05-12 Rheinmetall Landsysteme Gmbh Safety workbench
US9631827B2 (en) 2010-06-10 2017-04-25 Tecniplast S.P.A. Laminar air flow cage changing cabin made of plastic material
ITMI20101050A1 (en) * 2010-06-10 2011-12-11 Tecniplast Spa CHANGE CABIN LAMINAR AIR FLOW CAGE MADE OF PLASTIC MATERIAL
WO2011154502A1 (en) * 2010-06-10 2011-12-15 Tecniplast S.P.A. Laminar air flow cage changing cabin made of plastic material
WO2014024211A3 (en) * 2012-08-06 2014-04-10 Council Of Scientific & Industrial Research Bioreactor vessel for large scale growing of plants under aseptic conditions
US9872448B2 (en) 2012-08-06 2018-01-23 Council Of Scientific & Industrial Research Bioreactor vessel for large scale growing of plants under aseptic condition
EP3069800A1 (en) * 2015-03-17 2016-09-21 Kojair Tech Oy Biosafety cabinet and method of sterilising an object
CN105709523A (en) * 2016-01-29 2016-06-29 太仓东能环保设备有限公司 Sterilization air purifier
US10342191B2 (en) 2016-08-09 2019-07-09 Saed Technologies Ltd Method and apparatus for regeneration, acclimatization, and conditioning of plant propagules
WO2018027303A1 (en) * 2016-08-09 2018-02-15 Saed Technologies Ltd. Method and apparatus for regeneration, acclimatization, and conditioning of plant propagules
CN106439633B (en) * 2016-10-28 2019-05-28 湖南正海现代实验室设备有限公司 A kind of laboratory hood OLED headlamp
CN106439633A (en) * 2016-10-28 2017-02-22 湖南正海现代实验室设备有限公司 OLED (Organic Light Emitting Diode) illuminating lamp for ventilation cabinet of laboratory
CN108050589A (en) * 2017-11-24 2018-05-18 苏州赛易特环保科技有限公司 A kind of portable air cleaning unit
CN111687163A (en) * 2019-03-14 2020-09-22 安徽一洋实验设备有限公司 Environment-friendly self-loopa ventilation medicine cabinet
CN110252040A (en) * 2019-07-30 2019-09-20 淮北雷德机电科技有限公司 A kind of environmental protection pneumatic filter
CN113274223A (en) * 2021-05-21 2021-08-20 吉林大学 Sterile laminar flow chamber for aplastic anemia patients
GR1010197B (en) * 2021-05-21 2022-03-17 Ευσταθιος Ελευθεριου Τσατσαλακης Wheeled device for air sterilization and surface disinfection
RU209286U1 (en) * 2021-11-16 2022-03-14 Людмила Владимировна Матвеенко Air recirculator ultraviolet bactericidal

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AU6630601A (en) 2002-12-03
AU782243B2 (en) 2005-07-14
MY129354A (en) 2007-03-30

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