WO2002089498A3 - Selection method - Google Patents

Selection method Download PDF

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Publication number
WO2002089498A3
WO2002089498A3 PCT/US2002/013839 US0213839W WO02089498A3 WO 2002089498 A3 WO2002089498 A3 WO 2002089498A3 US 0213839 W US0213839 W US 0213839W WO 02089498 A3 WO02089498 A3 WO 02089498A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
selection method
methods
selecting
Prior art date
Application number
PCT/US2002/013839
Other languages
French (fr)
Other versions
WO2002089498A2 (en
Inventor
Yen Choo
Armin Sepp
Michael Moore
Original Assignee
Gendaq Ltd
Yen Choo
Armin Sepp
Michael Moore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gendaq Ltd, Yen Choo, Armin Sepp, Michael Moore filed Critical Gendaq Ltd
Priority to AU2002308561A priority Critical patent/AU2002308561A1/en
Publication of WO2002089498A2 publication Critical patent/WO2002089498A2/en
Publication of WO2002089498A3 publication Critical patent/WO2002089498A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6467—Granzymes, e.g. granzyme A (3.4.21.78); granzyme B (3.4.21.79)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein are methods for selecting nucleic acid molecules of interest from libraries of nucleic acid molecules. Also provided are methods for selecting nucleic acid molecules that encode polypeptides that are fused to nucleic acid binding peptide sequences.
PCT/US2002/013839 2001-04-30 2002-04-30 Selection method WO2002089498A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002308561A AU2002308561A1 (en) 2001-04-30 2002-04-30 Selection method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0110549.3 2001-04-30
GB0110549A GB0110549D0 (en) 2001-04-30 2001-04-30 Selection method

Publications (2)

Publication Number Publication Date
WO2002089498A2 WO2002089498A2 (en) 2002-11-07
WO2002089498A3 true WO2002089498A3 (en) 2004-03-18

Family

ID=9913739

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/013839 WO2002089498A2 (en) 2001-04-30 2002-04-30 Selection method

Country Status (3)

Country Link
AU (1) AU2002308561A1 (en)
GB (1) GB0110549D0 (en)
WO (1) WO2002089498A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008103900A2 (en) 2007-02-23 2008-08-28 New England Biolabs, Inc. Selection and enrichment of proteins using in vitro compartmentalization
US20230212555A1 (en) * 2020-02-28 2023-07-06 Synvitrobio, Inc. Methods and compositions for identifying traits using a cell-free system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002536A1 (en) * 1990-08-02 1992-02-20 The Regents Of The University Of Colorado Systematic polypeptide evolution by reverse translation
US5270163A (en) * 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5747253A (en) * 1991-08-23 1998-05-05 Isis Pharmaceuticals, Inc. Combinatorial oligomer immunoabsorbant screening assay for transcription factors and other biomolecule binding
WO2001025249A1 (en) * 1999-10-01 2001-04-12 Zhongping Yu Compositions and methods for identifying polypeptides and nucleic acid molecules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270163A (en) * 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
WO1992002536A1 (en) * 1990-08-02 1992-02-20 The Regents Of The University Of Colorado Systematic polypeptide evolution by reverse translation
US5747253A (en) * 1991-08-23 1998-05-05 Isis Pharmaceuticals, Inc. Combinatorial oligomer immunoabsorbant screening assay for transcription factors and other biomolecule binding
WO2001025249A1 (en) * 1999-10-01 2001-04-12 Zhongping Yu Compositions and methods for identifying polypeptides and nucleic acid molecules

Also Published As

Publication number Publication date
WO2002089498A2 (en) 2002-11-07
AU2002308561A1 (en) 2002-11-11
GB0110549D0 (en) 2001-06-20

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