WO2002083909A3 - Recombinant nucleotide sequence coding for a gene - Google Patents

Recombinant nucleotide sequence coding for a gene Download PDF

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Publication number
WO2002083909A3
WO2002083909A3 PCT/NL2002/000252 NL0200252W WO02083909A3 WO 2002083909 A3 WO2002083909 A3 WO 2002083909A3 NL 0200252 W NL0200252 W NL 0200252W WO 02083909 A3 WO02083909 A3 WO 02083909A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
nucleotide sequence
sequence coding
expressed
recombinant nucleotide
Prior art date
Application number
PCT/NL2002/000252
Other languages
French (fr)
Other versions
WO2002083909A2 (en
Inventor
Bastiaan Jan Herman Jansen
Steensel Maurice Adrianus Van
Original Assignee
Multigen Holding S A
Bastiaan Jan Herman Jansen
Steensel Maurice Adrianus Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Multigen Holding S A, Bastiaan Jan Herman Jansen, Steensel Maurice Adrianus Van filed Critical Multigen Holding S A
Priority to AU2002307630A priority Critical patent/AU2002307630A1/en
Publication of WO2002083909A2 publication Critical patent/WO2002083909A2/en
Publication of WO2002083909A3 publication Critical patent/WO2002083909A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/50—Vectors for producing vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a nucleotide sequence that codes for a gene that may be expressed at a desired moment and on a desired site in an organism into which the gene has been inserted. To this end a sequence has been integrated into an intron of the gene with the result that the gene is not expressed, or is expressed in an inactive form. The intron further comprises sequences that allow this disrupting factor to be removed, so that the gene can be expressed.
PCT/NL2002/000252 2001-04-17 2002-04-17 Recombinant nucleotide sequence coding for a gene WO2002083909A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002307630A AU2002307630A1 (en) 2001-04-17 2002-04-17 Recombinant nucleotide sequence coding for a gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL1017852A NL1017852C2 (en) 2001-04-17 2001-04-17 Recombinant nucleotide sequence which encodes a gene.
NL1017852 2001-04-17

Publications (2)

Publication Number Publication Date
WO2002083909A2 WO2002083909A2 (en) 2002-10-24
WO2002083909A3 true WO2002083909A3 (en) 2002-12-27

Family

ID=19773243

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2002/000252 WO2002083909A2 (en) 2001-04-17 2002-04-17 Recombinant nucleotide sequence coding for a gene

Country Status (3)

Country Link
AU (1) AU2002307630A1 (en)
NL (1) NL1017852C2 (en)
WO (1) WO2002083909A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018096356A1 (en) * 2016-11-28 2018-05-31 Horizon Discovery Limited Methods for conditional gene knock-out

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19650714A1 (en) * 1996-12-06 1998-06-10 Melchner Harald Von Prof Dr Gene trap construct for the identification and isolation of genes
WO1998027207A1 (en) * 1996-12-18 1998-06-25 Targeted Genetics Corporation Recombinase-activatable aav packaging cassettes for use in the production of aav vectors
DE19834430A1 (en) * 1998-07-30 2000-02-03 Harald Von Melchner Self-deleting vectors for cancer therapy
WO2001005961A1 (en) * 1999-07-14 2001-01-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
EP1134287A1 (en) * 2000-03-08 2001-09-19 Universite De Geneve A system to control the expression of a given gene using another gene that encodes a polypeptide with recombinant activity

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19650714A1 (en) * 1996-12-06 1998-06-10 Melchner Harald Von Prof Dr Gene trap construct for the identification and isolation of genes
WO1998027207A1 (en) * 1996-12-18 1998-06-25 Targeted Genetics Corporation Recombinase-activatable aav packaging cassettes for use in the production of aav vectors
DE19834430A1 (en) * 1998-07-30 2000-02-03 Harald Von Melchner Self-deleting vectors for cancer therapy
WO2001005961A1 (en) * 1999-07-14 2001-01-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
EP1134287A1 (en) * 2000-03-08 2001-09-19 Universite De Geneve A system to control the expression of a given gene using another gene that encodes a polypeptide with recombinant activity

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
FORLINO A ET AL: "USE OF THE CRE/LOX RECOMBINATION SYSTEM TO DEVELOP A NON-LETHAL KNOCK-IN MURINE MODEL FOR OSTEOGENESIS IMPERFECTA WITH AN ALPHA1(I) G349C SUBSTITUTION VARIABILITY IN PHENOTYPE IN BRTLIV MICE", BIOLOGICAL CHEMISTRY, XX, XX, vol. 274, no. 53, 31 December 1999 (1999-12-31), pages 37923 - 37931, XP001020393, ISSN: 1431-6730 *
INDRA ARUP KUMAR ET AL: "Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: Comparison of the recombinase activity of the tamoxifen-inducible Cre-ERT and Cre-ERT2 recombinases", NUCLEIC ACIDS RESEARCH, IRL PRESS LTD., OXFORD, GB, vol. 27, no. 22, 15 November 1999 (1999-11-15), pages 4324 - 4327, XP002144711, ISSN: 0305-1048 *
SORIANO P: "Generalized lacZ expression with the ROSA26 Cre reporter strain", NATURE GENETICS, NATURE AMERICA, NEW YORK, US, vol. 21, January 1999 (1999-01-01), pages 70 - 71, XP002206126, ISSN: 1061-4036 *
UTOMO AHMAD R H ET AL: "Temporal, spatial, and cell type-specific control of Cre-mediated DNA recombination in transgenic mice.", NATURE BIOTECHNOLOGY, vol. 17, no. 11, November 1999 (1999-11-01), pages 1091 - 1096, XP002186984, ISSN: 1087-0156 *
VASIOUKHIN VALERI ET AL: "The magical touch: Genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, no. 15, 20 July 1999 (1999-07-20), pages 8551 - 8556, XP002144707, ISSN: 0027-8424 *
ZHANG YONG ET AL: "Induction of the antigen receptor expression on B lymphocytes results in rapid competence for signaling of SLP-65 and Syk.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 17, no. 24, 15 December 1998 (1998-12-15), pages 7304 - 7310, XP002186985, ISSN: 0261-4189 *

Also Published As

Publication number Publication date
AU2002307630A1 (en) 2002-10-28
WO2002083909A2 (en) 2002-10-24
NL1017852C2 (en) 2002-10-29

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