WO2002081492A1 - Human gene critical to fertility - Google Patents

Abstract

Disclosed herein are novel nucleic acid and protein sequences that are essential to fertility. In particular, human Mater genomic, cDNA and protein sequences are provided, as are fragments and variants thereof. Functional MATER is required for female fertility; zygotes that arise from Mater null oocytes do not progress beyond the two-cell stage. Methods are described for using Mater molecules in diagnoses, prognosis, and treatment of infertility and reduced fertility, and kits related to such methods. Also provided are methods for using Mater as a contraceptive agent. The disclosure also describes compounds involved in such methods, and the identification of such compounds.

Description

HUMAN GENE CRITICAL TO FERTILITY

REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of PCT Application Number PCT/US01/10981, filed April 4, 2001, which claims the benefit of U.S. Provisional Application 60/241,510, filed October 18, 2000, both of which are incorporated herein by reference in their entirety.

FIELD

The present disclosure is generally related to fertility, including the mechanisms controlling it, diseases that arise from defects in such mechanisms, and methods of influencing (either inhibiting or enhancing) fertility.

BACKGROUND

Premature ovarian failure (POF) is a term used to describe certain types of infertility in women. As many as 1% of all women in the United States are thought to be afflicted with POF, which manifests as menopausal-type symptoms, including infertility, in women under the age of 40. Many different diseases and conditions can cause POF, including underlying chromosomal defects (e.g., X-chromosome fragility), chemotherapy, or radiation treatment. Autoimmunity is a well- established mechanism of premature ovarian failure (see Yan et al, J W omens Health Gend Based Med. 9:275-87, 2000; and Kalantaridou & Nelson, J Am Med Womens Assoc. 53:18-20, 1998). In autoimmune infertility, a woman's ovaries are attacked by cells of her own immune system, leading to a condition known as autoimmune oophoritis (inflammation ofthe ovary). Autoimmune disease can develop in response to a single inciting antigen and then spread to involve other antigenic molecules ofthe same organ (Kaufman, Nature 366:69-72, 1993). Therefore, identifying the autoantigen target in an organ-specific autoimmune disease is essential to understanding its pathogenesis.

An experimental animal (mouse) model has been used to gain insight into the mechanisms of human autoimmune oophoritis. Removal ofthe thymus (thymectomy) in neonatal mice (about three days old) induces experimental autoimmune oophoritis in certain strains of mice (Taguchi et al, Clin Exp Immunol. 42:324-331, 1980). This experimentally induced condition leads to the production of high levels of anti-ooplasm antibodies and sterility, accompanied by follicular degeneration; the progression ofthe condition appears to closely parallel human autoimmune oophoritis (Kalantaridou & Nelson, J Am Med Womens Assoc. 53:18-20, 1998).

Maternal products control the developmental program until embryonic genome activation takes place. Maternal effect genes that are important in early embryonic development have been well documented in Drosophila mdXenopus (Morisato & Anderson, Annu. Rev. Genet. 29:371-399, 1995; Newport & Kirschner, Cell 30:687-696,1982), but their presence has only been inferred in mammals (Gardner, Hum. Reprod. Update 2:1-27, 1999). In mice, embryonic transcription is first detected in the late 1-cell 2ygote stage and is required for development beyond the 2-cell stage (Schultz, Bioessays 15:531-538, 1993; Flac et al., EMBO J. 1:681-686, 1982; Latham et al., Mol. Reprod. Dev. 35:140-150, 1993). The factors governing this transition from the maternal to the embryonic genome are unknown. A critical transition in development occurs with the switch from dependence on proteins stored in the egg to those that result from activation ofthe embryonic genome. This shift which occurs at the two-cell stage in mice is dependent on maternal factors. Gene transcription and protein translation are active during murine oogenesis and RNAs and proteins accumulate within oocytes. However, germ cells becomes transcriptionally inactive late in oogenesis and much ofthe maternal RNA is degraded during meiotic maturation and ovulation ofthe egg into the oviduct. Thus, few maternal gene products persist past the two-cell embryo stage and none have been demonstrated directly to affect early development (Schultz, Bioessays 15, 531-538, 1993; Gardner, Hum. Reprod. Update 2, 3-27, 1996).

SUMMARY OF THE DISCLOSURE

Described herein is the human MATER protein, an approximately 135 kDa (predicted estimated molecular weight) cytoplasmic protein expressed in mammalian oocytes that is required for female fertility. Zygotes that arise from a MATER null oocyte do not progress beyond the two-cell stage. Some embodiments include an isolated human MATER protein predicted to have an estimated molecular weight of about 125 kDa to about 135 kDa, in some embodiments more particularly about 134.2 kDa. For instance, this estimated molecular weight may be obtained by SDS- polyacrylamide gel electrophoresis and Western blotting, for instance using an antibody raised against a C-terminal peptide of murine MATER {e.g., residues 1093 to 1111 of SEQ ID NO: 6). MATER proteins include an amino acid sequence as set forth in SEQ ID NOs: 2 or 4 or 24, or a sequence having at least 65% sequence identity (e.g., at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or more) with SEQ ID NOs: 2 or 4 or 24. Certain specific examples of such proteins may contain one or more conservative variants within such sequences. Certain ofthe provided human MATER proteins have MATER protein biological activity, for instance in that they can complement a MATER null phenotype.

One specific embodiment is thus an isolated human MATER protein predicted to have an estimated molecular weight of about 135 kDa, wherein the human MATER protein comprises amino acid sequences as set forth in SEQ ID NO: 2 and/or SEQ ID NO: 4 and/or SEQ ID NO: 24, the protein is an oocyte cytoplasm-specific autoantigen associated with autoimmune infertility, and it can complement a Mater null phenotype by permitting progression of an embryo beyond the two-cell stage.

Other embodiments are variant MATER proteins, having at least one variant amino acid compared to wildtype. Examples of such variant proteins include proteins where at least one variant amino acid occurs within a recognized domain or region within the protein. In other examples, at least one variant amino acid occurs outside of a recognized domain or region ofthe MATER protein.

Still other examples are variants having at least one amino acid variant in and one outside of a recognized domain. Further embodiments are isolated nucleic acid molecules that encode a variant MATER protein, recombinant nucleic acid molecules that include a promoter sequence operably linked to a MATER nucleic acid molecule and cells transformed with such a recombinant nucleic acid molecule.

Methods of detecting a biological condition associated with an abnormal Mater nucleic acid or an abnormal MATER expression or an autoimmune response to MATER in a subject are also provided. Examples of such a biological condition include infertility (such as autoimmune infertility) or reduced fertility, or an increased susceptibility to infertility or reduced fertility. Also provided are kits for use with such methods.

A further embodiment includes methods of screening for a compound useful in influencing

MATER-mediated fertility in a mammal. The foregoing and other features and advantages ofthe invention will become more apparent from the following detailed description of several embodiments, which proceeds with reference to the accompanying figures.

BRIEF DESCRIPTION OF THE FIGURES FIG. 1 shows two digitized gels, and a series of digitized micrographs, detailing the developmental expression of murine MATER.

FIG. 1A is a digitized image of a Northern blots showing RNAse protection of Mater and ZP3 transcripts. RNase protection assays were performed using total RNA from 50 oocytes, eggs or embryos. ³²P-labeled antisense probes and protected fragment lengths for Mater and ZP3 were 180/139 nt and 257/205 nt, respectively.

FIG. IB is a digitized image of a Western blot, showing MATER protein (approximately 125 kD) amounts in the indicated murine cell types. MATER protein was assayed in 25 oocytes, eggs or embryos by immunoblotting with monospecific antisera to MATER.

FIG. 1C is a series of digitized micrographs, showing the cellular localization of MATER protein. Protein localization was determined using fluorescent labeled antibody, by imaging oocytes, eggs and embryos with laser-scanning confocal microscopy alone (upper images) or after superimposition on a Nomarski image (lower images). Scale bar, 50 μm.

FIG. 2 shows the characterization of targeted Mater locus disruptions. FIG. 2A is a schematic representation ofthe normal murine Mater allele (upper), the targeting construct (middle) and the null allele (bottom). The 5' and 3' probes used to assess targeting were outside the region of DNA homology. FIG. 2B is a series of digitized Northern blots, showing that the Mater null allele was detected in Xbal digested ES cell DNA as a 3.5 kb fragment with the 5' (left) or as a 4.0 kb fragment with the 3' (middle) and neo (right) probes.

FIG. 2C is a pair of digitized Northern blots, showing that Mater transcripts were detected in normal and heterozygous (half the abundance of normal), but not in homozygous null ovaries (left panel). ZP3 transcripts (control) were present in all three genotypes (right panel).

FIG. 3 is a series of digitized micrographs and corresponding bar graphs, showing the in vivo development defects of embryos derived from Mater null female mice.

After gonadotrophin induced ovulation, female mice were mated with normal males and oviducts from normal (FIG. 3A, 3D, 3G, and 3J) or Mater null (FIG. 3B, 3E, 3H, and 3K) females were flushed one (FIG. 3A and 3B), two (FIG. 3D and 3E), three (FIG. 3G and 3H) and four (FIG. 3J and 3K) days later. The unfixed embryos were photographed using Nomarski optics. The arrows point to pronuclei in 1-cell zygotes (FIG. 3A and 3B). Scale bar, 50 μm.

The bar graphs indicate developmental progress ofthe average number of embryos derived from Mater null (■) and normal (D) females at one (FIG. 3C), two (FIG. 3F), three (FIG. 31), and four (FIG. 3L) days after mating. Each bar represents the average of 4-5 experiments ± s.e.m.

FIG. 4 shows that de novo transcription and translation occurs in murine embryos lacking Mater.

FIG. 4A-4F show digitized micrograph images of murine embryos. Newly synthesized RNA was measured by BrUTP incorporation into the nucleus of one- (FIG. 4A and 4B) and 2-cell (FIG. 4C and 4D) embryos derived from Mater null (FIG. 4A and 4C) and normal (FIG. 4B and 4D) females using laser-scanning confocal microscopy and a monoclonal antibody to BrUTP.

FIG. 4G is a bar graph, showing the quantity of BrUTP incorporated in 2-cell embryos with (+) and without (-) Mater using arbitrary fluorescence units after subtraction of that obtained with uninjected controls (FIG. 4E and 4F). Scale bar, 50 μm.

FIG. 4H is a pair of digitized northern blots, showing RNase protection assays of 28S ribosomal RNA using total RNA from 50 growing oocytes, eggs and 2-cell embryos isolated from females with (+) and without (-) Mater. ³²P-labeled antisense probes and protected fragment lengths for 28S-rRNA were 153 nt and 115 nt, respectively. FIG. 41 is a series of digitized fluorographs, showing de novo protein synthesis in 1-cell zygotes (left panel) and 2-cell embryos (middle and right panels) that do (+) or do not (-) contain Mater. Each lane contained proteins from 10 embryos that were dissolved in sample buffer directly (left and middle panels) or after partial purification extraction of TRC (65-75 kDa) and p35 (right panels). FIG. 5 shows ovarian histology, eggs and the number of eggs produced from Mater null females. The number of ovulated eggs and their morphology indicating maturation or degeneration were not remarkably different between normal and Mater null mice. FIG. 5A and 5B are digitized images of ovarian slices from a 4 week old (FIG. 5A) and 8 week old (FIG. 5B) Mater null mouse. The 4 week old Mater null ovarian histology (FIG. 5A) displays different stages of normal ovarian follicles with oogenesis, which are indistinguishable from normal ovarian histology. In the 8 week old sample (FIG. 5B), there are a number of corpora lutea indicating normal spontaneous ovulation, similar to follicular luteinization in the wild-type mouse ovary.

FIG. 5C is a digitized photograph of ovulated eggs from the Mater null mice, produced in response to exogenous PMSG and hCG. Morphology appears similar to that of ovulated eggs from wild-type mice. FIG. 5D is a bar graphs showing the numbers (mean ± s.e.m.) ofthe ovulated eggs from the

Mater null and wild-type mice.

FIG. 6 is a digitized image comparing the gross morphological appearance ofthe uterus and ovaries of normal and Mater null mice. Ovaries and uteri if Mater null female mice (right) were indistinguishable from the ovaries and uteri from the wild-type female mice (left). FIG. 7 is an alignment ofthe amino acid sequence of human MATER (SEQ ID NO: 24) and murine MATER (SEQ ID NO: 6) proteins. Identical amino acids are identified; similar amino acids are indicated with a plus (+) symbol.

FIG. 8 is illustrates human MATER mRNA and its oocyte-specific expression.

FIG. 8A is a digitized image of a Northern hybridization of human MATER. Total human ovarian RNAs were prepared from women at age 19 (lane 1) and 26 (lane 2) years, as described herein. Ten micrograms of each RNA were separated by gel-electrophoresis and transferred onto a nitrocellulose membrane. The blot was probed with ³²P-labeled human MATER cDNA. RNA molecular sizes (kb) are indicated on the left of panel.

FIG. 8B and 8C are digitized images of agarose gels, showing analysis ofthe indicated transcripts by RT-PCR. Using the Human Rapid-Scan Gene Expression Panel (Origene Inc) and specific primers, cDNAs at 100X concentration were used to amplify human β-actin (FIG. 8B), while cDNA at 1000X concentration were used to amplify human MATER (FIG. 8C). The cDNAs were synthesized from the RNAs ofthe human brain (lane 1), heart (2), kidney (3), spleen (4), liver (5), colon (6), lung (7), small intestine (8), muscle (9), stomach (10), testis (11), placenta (12), salivary gland (13), thyroid (14), adrenal gland (15), pancreas (16), ovary (17), uterus (18), prostate (19), skin (20), blood leucocytes (21), bone marrow (22), fetal brain (23), fetal liver (24). Lane 25 and 26 serve as a positive control (human ovarian cDNA, Invitrogen) and a negative control (without cDNA), respectively. PCR products were separated by 2% agarose gel-electrophoresis and visualized by 0.1% ethidium bromide. Amplification ofthe PCR products of human β-actin (640 bp) and human MATER (280 bp) is indicated on the right, respectively.

FIG. 8D is an image of a Southern hybridization blot prepared from the agarose gel separating the human MATER PCR product in FIG. 8C, and probed with ³²P-labeled human MATER cDNA. Hybridization signals were detected by autoradiography. The arrow indicates the position of PCR product hybridization of human MATER (280 bp) in the control (lane 25) and ovary samples (lane 17).

FIG. 8E and 8F illustrate in situ hybridization of MATER cDNA. ³⁵S-labeled antisense and sense probes were synthesized in vitro transcription using the human MATER cDNA as templates. The frozen human ovarian sections were hybridized with the antisense probe (FIG. 8E) and sense probe (FIG. 8F). Dark-field images are displayed, and an arrow indicates the locations ofthe oocytes. Scale bar, 100 μm.

FIG. 9 is a series of digitized micrographs, showing oocyte-specific expression of Mater transcripts in human by in situ hybridization. Both [³³S]-labeled antisense and sense probes were synthesized by in vitro transcription using the cloned human Mater cDNA as a template. The frozen human ovarian sections were hybridized with the radiolabeled sense (FIG. 9 A and 9C) and antisense (FIG. 9B and 9D) probes. The slides were stained with hematoxylin and eosin. For each probe, bright-field (FIG. 9A and 9B) and dark-field (FIG. 9C and 9D) images are shown.

FIG. 10 is a pair of digitized micrographs, showing oocyte-specific expression of human MATER protein. Frozen human ovarian sections were incubated with rabbit antisera (1 :200) against a C-terminal peptide of mouse MATER protein, and FITC-conjugated goat anti-rabbit IgG antisera were used as the second antibody to detect human MATER protein in the oocyte (FIG. 10A). The corresponding phase contrast image is shown in FIG. 10B.

FIG. 11 illustrates human MATER protein expression in oocytes. FIG. 11A and 1 IB are digitized micrographs showing immunohistofluorescence of human

MATER in ovarian sections. Frozen human ovarian sections were incubated with rabbit antisera (1 :200) against the mouse MATER peptide. FITC-conjugated goat anti-rabbit IgG antibody was used as a second antibody to detect the primary antibody bound MATER protein. Tissue sections were visualized by a fluorescence microscopy (FIG. 11 A). Corresponding phase contrast is shown in the FIG. 1 IB. Arrows indicate the location of oocytes. Scale bar: 100 μm.

FIG. 11C is a protein immunoblot of human MATER. Solubilized proteins of human eggs (lane 1, 10 eggs and lane 2, 15 eggs) were separated by 3-8% Tris- Acetate SDS-PAGE electrophoresis. A protein blot was incubated with rabbit anti-mouse MATER peptide antisera (1 : 1000). The ECL detection kit with HRP-coηjugated goat anti-rabbit IgG antibody was used to detect the blot-bound primary antibodies. Approximate protein molecular masses (kDa) are indicated on the left. Human MATER protein is indicated by an arrow at -134 kDa.

FIG. 12 shows the nucleotide (cDNA; SEQ ID NO: 23) and amino acid (SEQ ID NO: 24) sequences of human MATER. The nucleotides (3885 nts) from 5' are displayed on the top line and their numbers are indicated on the right. The initiation code (ATG) and termination code (TGA) is underlined (solid), respectively. The polyadenylation signal (AATAAA) is underlined with a dashed line. The nucleotides at the 5' end ofthe non-coding region remain to be determined. The deduced amino acid sequence (1200 residues) is under the nucleotide sequence and numbered on the right. Some structural motifs present in the protein sequence are shown in FIG. 14B. These sequence data have been submitted to GenBank under an accession number AY054986.

FIG. 13 shows Dotplot analyses of human and mouse MATER amino acid sequences. Human MATER amino acid sequence was compared with itself (FIG 13 A) and the mouse MATER amino acid sequence (FIG. 13B). The center diagonal line from the left to the right represents the direct correspondence ofthe two sequences ofthe same protein from amino- to carboxyl-terminus. Identities are shown by the dots. The short diagonal lines parallel to the center diagonal line are present at the carboxyl-terminus and a region close to the amino-terminus, reflecting repeated amino acid sequences at these regions. Display ofthe dots and short parallel lines delineate similarities of amino acid sequences between human MATER (1200 residues) and mouse MATER (1111 residues), reflecting homologies ofthe composition and structure ofthe MATER protein ofthe two species.

FIG. 14 illustrates the results of computational analysis of structural motifs in human MATER. FIG. 14A is a Kyte & Doolittle hydropathy plot of human MATER amino acid sequence.

Hydrophilic repeats at amino-terminus (32-304 amino acids) contain a short hydrophobic region (104- 136 amino acids). At the carboxyl-terminus, there is a short hydrophilic region (1184-1200 amino acids).

FIG. 14B is a schematic representation of structural motifs of human MATER. The horizontal line from amino- to carboxyl-terminus represents the entire peptide chain of human

MATER (1200 residues). The opened boxes represent structural motifs and their positions on the peptide chain. The protein contains a domain of PAAD_DAPIN at the amino-terminus and six domains of leucine-rich repeats (LRR) at the carboxyl-terminus. Both molecular domains are for protein-protein interactions. In addition, there are motifs with potentials for ATP/GTP-binding (P- loop), nuclear translocation (NLS_BP) and aldo/keto reductase activity (aldo_ket_red). Locations of these motifs are indicated in the numbers following the particular motifs.

FIG. 15 is a pair of schematic representations of exon-intron maps of human MATER (FIG. 15A) and mouse Mater (FIG. 15B). The exon-intron map of human MATER (-63 kbp) was compared with the mouse Mater (-32 kbp) exon-intron map (Tong et al. , Mammal Genome 11:281, 2000). Both gene loci have fifteen exons that are indicated by the Arabic numbers on top of each ofthe vertical bars representing the exons. The horizontal solid lines between exons represent introns, and the horizontal dashed lines at 5' ends represent the undetermined 5' ends of both gene loci. Scale bar (2 kbp) is shown under the map of human MATER. SEQUENCE LISTING

The nucleic and amino acid sequences listed in the accompanying sequence listing are shown using standard letter abbreviations for nucleotide bases, and three letter code for amino acids, as defined in 37 C.F.R. 1.822. Only one strand of each nucleic acid sequence is shown, but the complementary strand is understood as included by any reference to the displayed strand. In the accompanying sequence listing:

SEQ ID NO: 1 shows the nucleic acid sequence of human Mater cDNA fragment 1.

SEQ ID NO: 2 shows the amino acid sequence ofthe human MATER peptide encoded by fragment 1 (SEQ ID NO: 1).

SEQ ID NO: 3 shows the nucleic acid sequence of human Mater cDNA fragment 2.

SEQ ID NO: 4 shows the amino acid sequence ofthe human MATER peptide encoded by fragment 2 (SEQ ID No: 3).

SEQ ID NO: 5 shows the nucleic acid sequence of murine Mater cDNA (GenBank Accession number NM 011860.1 and AF074018; individual exons are also listed in AF143559- AF143573).

SEQ ID NO: 6 shows the amino acid sequence ofthe murine MATER protein (GenBank Accession number NP_035990).

SEQ ID NOs: 7-22 shows several synthetic ohgonucleotides useful as probes and/or primers. SEQ ID NO: 23 shows the nucleic acid sequence ofthe human Mater cDNA, and the amino acid sequence so encoded.

SEQ ID NO: 24 shows the amino acid sequence ofthe human MATER protein.

SEQ ID NOs: 25-38 are oligonucleotide sequences that overlap the intron-exon boundaries in the human Mater gene. In order, these ohgonucleotides correspond to the splice junctions that define exons 1-15, as described more fully herein.

SEQ ID NOs: 39 and 40 are human β-actin specific primers.

SEQ ID NOs: 41 and 42 are human MATER-specific primers.

DETAILED DESCRIPTION OF THE DISCLOSURE I. Abbreviations

ES: embryonic stem

Mater. Maternal Antigen That Embryos Require OP1: Ooplasm-specific Protein 1 POF: Premature Ovarian Failure TRC: Transcription Related Complex ZP3: zona pellucida glycoprotein 3

//. Terms

Unless otherwise noted, technical terms are used according to conventional usage. Definitions of common terms in molecular biology may be found in Benjamin Lewin, Genes V, published by Oxford University Press, 1994 (ISBN 0-19-854287-9); Kendrew et al. (eds.), The Encyclopedia of Molecular Biology, published by Blackwell Science Ltd., 1994 (ISBN 0-632-02182- 9); and Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, published by VCH Publishers, Inc., 1995 (ISBN 1-56081-569-8). In order to facilitate review ofthe various embodiments ofthe invention, the following explanations of specific terms are provided:

Abnormal: Deviation from normal characteristics. Normal characteristics can be found in a control, a standard for a population, etc. For instance, where the abnormal condition is an autoimmune disease condition, such as autoimmune infertility, sources of normal characteristics might include an individual who is not suffering from the autoimmune disorder, a population standard of individuals believed not to be suffering from the disease, etc.

In some examples, abnormal may refer to a condition that is associated with a disease. The term "associated with" includes an increased risk of developing the disease as well as the disease itself. For instance, a certain abnormality (such as an abnormality in an Mater nucleic acid or

MATER protein expression) can be described as being associated with the biological conditions of altered (e.g., reduced) fertility and tendency to develop autoimmune infertility.

An abnormal nucleic acid, such as an abnormal Mater nucleic acid, is one that is different in some manner to a normal (wildtype) nucleic acid. Such abnormality includes but is not necessarily limited to: (1) a mutation in the nucleic acid (such as a point mutation (e.g., a single nucleotide polymorphism) or short deletion or duplication of a few to several nucleotides); (2) a mutation in the control sequence(s) associated with that nucleic acid such that replication or expression ofthe nucleic acid is altered (such as the functional inactivation of a promoter); (3) a decrease in the amount or copy number ofthe nucleic acid in a cell or other biological sample (such as a deletion ofthe nucleic acid, either through selective gene loss or by the loss of a larger section of a chromosome or under expression ofthe mRNA); and (4) an increase in the amount or copy number ofthe nucleic acid in a cell or sample (such as a genomic amplification of part or all ofthe nucleic acid or the overexpression of an mRNA), each compared to a control or standard. It will be understood that these types of abnormalities can co-exist in the same nucleic acid or in the same cell or sample; for instance, a genomic-amplified nucleic acid sequence may also contain one or more point mutations. In addition, it is understood that an abnormality in a nucleic acid may be associated with, and in fact may cause, an abnormality in expression o the corresponding protein.

Abnormal protein expression, such as abnormal MATER protein expression, refers to" expression of a protein that is in some manner different to expression ofthe protein in a normal (wildtype) situation. This includes but is not necessarily limited to: (1) a mutation in the protein such that one or more ofthe amino acid residues is different; (2) a short deletion or addition of one or a few amino acid residues to the sequence ofthe protein; (3) a longer deletion or addition of amino acid residues, such that an entire protein domain or sub-domain is removed or added; (4) expression of an increased amount ofthe protein, compared to a control or standard amount; (5) expression of an decreased amount ofthe protein, compared to a control or standard amount; (6) alteration ofthe subcellular localization or targeting ofthe protein; (7) alteration ofthe temporally regulated expression ofthe protein (such that the protein is expressed when it normally would not be, or alternatively is not expressed when it normally would be); and (8) alteration ofthe localized (e.g., organ or tissue specific) expression ofthe protein (such that the protein is not expressed where it would normally be expressed or is expressed where it normally would not be expressed), each compared to a control or standard.

Controls or standards appropriate for comparison to a sample, for the determination of abnormality, include samples believed to be normal as well as laboratory values, even though possibly arbitrarily set, keeping in mind that such values may vary from laboratory to laboratory. Laboratory standards and values may be set based on a known or determined population value and may be supplied in the format of a graph or table that permits easy comparison of measured, experimentally determined values. Antisense, Sense, and Antigene: Double-stranded DNA (dsDNA) has two strands, a 5' ->

3' strand, referred to as the plus strand, and a 3' -> 5' strand (the reverse complement), referred to as the minus strand. Because RNA polymerase adds nucleic acids in a 5' -> 3' direction, the minus strand ofthe DNA serves as the template for the RNA during transcription. Thus, the RNA formed will have a sequence complementary to the minus strand and identical to the plus strand (except that U is substituted for T).

Antisense molecules are molecules that are specifically hybridizable or specifically complementary to either RNA or the plus strand of DNA. Sense molecules are molecules that are specifically hybridizable or specifically complementary to the minus strand of DNA. Antigene molecules are either antisense or sense molecules directed to a dsDNA target. Binding or stable binding: An oligonucleotide binds or stably binds to a target nucleic acid if a sufficient amount ofthe oligonucleotide forms base pairs or is hybridized to its target nucleic acid, to permit detection of that binding. Binding can be detected by either physical or functional properties ofthe target: oligonucleotide complex. Binding between a target and an oligonucleotide can be detected by any procedure known to one skilled in the art, including both functional and physical binding assays. Binding may be detected functionally by determining whether binding has an observable effect upon a biosynthetic process such as expression of a gene, DNA replication, transcription, translation, and the like.

Physical methods of detecting the binding of complementary strands of DNA or RNA are well known in the art, and include such methods as DNase I or chemical footprinting, gel shift and affinity cleavage assays, Northern blotting, dot blotting and light absorption detection procedures.

For example, one method that is widely used, because it is so simple and reliable, involves observing a change in light absoφtion of a solution containing an oligonucleotide (or an analog) and a target nucleic acid at 220 to 300 nm as the temperature is slowly increased. Ifthe oligonucleotide or analog has bound to its target, there is a sudden increase in absorption at a characteristic temperature as the oligonucleotide (or analog) and target disassociate from each other, or melt.

The binding between an oligomer and its target nucleic acid is frequently characterized by the temperature (T_m) at which 50% ofthe target sequence remains hybridized to a perfectly matechd probe or complementary strand. A higher (T_ra) means a stronger or more stable complex relative to a complex with a lower (T_m). cDNA (complementary DNA): A piece of DNA lacking internal, non-coding segments (introns) and transcriptional regulatory sequences. cDNA may also contain untranslated regions (UTRs) that are responsible for translational control in the corresponding RNA molecule. cDNA is usually synthesized in the laboratory by reverse transcription from messenger RNA extracted from cells.

DNA (deoxyribonucleic acid): DNA is a long chain polymer which comprises the genetic material of most living organisms (some viruses have genes comprising ribonucleic acid (RNA)). The repeating units in DNA polymers are four different nucleotides, each of which comprises one of the four bases, adenine (A), guanine (G), cytosine (C), and thymine (T) bound to a deoxyribose sugar to which a phosphate group is attached. Triplets of nucleotides (referred to as codons) code for each amino acid in a polypeptide, or for a stop signal. The term codon is also used for the corresponding (and complementary) sequences of three nucleotides in the mRNA into which the DNA sequence is transcribed. Unless otherwise specified, any reference to a DNA molecule is intended to include the reverse complement of that DNA molecule. Except where single-strandedness is required by the text herein, DNA molecules, though written to depict only a single strand, encompass both strands of a double-stranded DNA molecule. Thus, a reference to the nucleic acid molecule that encodes a specific protein, or a fragment thereof, encompasses both the sense strand and its reverse complement. Thus, for instance, it is appropriate to generate probes or primers from the reverse complement sequence ofthe disclosed nucleic acid molecules.

Deletion: The removal of a sequence of DNA, the regions on either side ofthe removed sequence being joined together.

Gene amplification or genomic amplification: An increase in the copy number of a gene or a fragment or region of a gene or associated 5 ' or 3' region, as compared to the copy number in normal tissue. An example of a genomic amplification is an increase in the copy number of an oncogene. A "gene deletion" is a deletion of one or more nucleic acids normally present in a gene sequence and, in extreme examples, can include deletions of entire genes or even portions of chromosomes. Hybridization: Ohgonucleotides and their analogs hybridize by hydrogen bonding, which includes Watson-Crick, Hoogsteen or reversed Hoogsteen hydrogen bonding, between complementary bases. Generally, nucleic acid consists of nitrogenous bases that are either pyrimidines (cytosine (C), uracil (U), and thymine (T)) or purines (adenine (A) and guanine (G)). These nitrogenous bases form hydrogen bonds between a pyrimidine and a purine, and the bonding of the pyrimidine to the purine is referred to as "base pairing." More specifically, A will hydrogen bond to T or U, and G will bond to C. "Complementary" refers to the base pairing that occurs between to distinct nucleic acid sequences or two distinct regions ofthe same nucleic acid sequence. "Specifically hybridizable" and "specifically complementary" are terms that indicate a sufficient degree of complementarity such that stable and specific binding occurs between the oligonucleotide (or its analog) and the DNA or RNA target. The oligonucleotide or oligonucleotide analog need not be 100% complementary to its target sequence to be specifically hybridizable. An oligonucleotide or analog is specifically hybridizable when binding ofthe oligonucleotide or analog to the target DNA or RNA molecule interferes with the normal function ofthe target DNA or RNA, and there is a sufficient degree of complementarity to avoid non-specific binding ofthe oligonucleotide or analog to non-target sequences under conditions where specific binding is desired, for example under physiological conditions in the case of in vivo assays or systems. Such binding is referred to as specific hybridization. Hybridization conditions resulting in particular degrees of stringency will vary depending upon the nature ofthe hybridization method of choice and the composition and length ofthe hybridizing nucleic acid sequences. Generally, the temperature of hybridization and the ionic strength (especially the Na⁺ concentration) ofthe hybridization buffer will determine the stringency of hybridization, though waste times also influence stringency. Calculations regarding hybridization conditions required for attaining particular degrees of stringency are discussed by Sambrook et al. (ed.), Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989, chapters 9 and 11, herein incorporated by reference.

For present purposes, "stringent conditions" encompass conditions under which hybridization will only occur if there is less than 25% mismatch between the hybridization molecule and the target sequence. "Stringent conditions" may be broken down into particular levels of stringency for more precise definition. Thus, as used herein, "moderate stringency" conditions are those under which molecules with more than 25% sequence mismatch will not hybridize; conditions of "medium stringency" are those under which molecules with more than 15% mismatch will not hybridize, and conditions of "high stringency" are those under which sequences with more than 10% mismatch will not hybridize. Conditions of "very high stringency" are those under which sequences with more than 6% mismatch will not hybridize.

In vitro amplification: Techniques that increases the number of copies of a nucleic acid molecule in a sample or specimen. An example of amplification is the polymerase chain reaction, in which a biological sample collected from a subject is contacted with a pair of oligonucleotide primers, under conditions that allow for the hybridization ofthe primers to nucleic acid template in the sample. The primers are extended under suitable conditions, dissociated from the template, and then re- annealed, extended, and dissociated to amplify the number of copies ofthe nucleic acid. The product of in vitro amplification may be characterized by electrophoresis, restriction endonuclease cleavage patterns, oligonucleotide hybridization or ligation, and/or nucleic acid sequencing, using standard techniques. Other examples of in vitro amplification techniques include strand displacement amplification (see U.S. Patent No. 5,744,311); transcription-free isothermal amplification (see U.S. Patent No. 6,033,881); repair chain reaction amplification (see WO 90/01069); ligase chain reaction amplification (see EP-A-320 308); gap filling ligase chain reaction amplification (see U.S. Patent No. 5,427,930); coupled ligase detection and PCR (see U.S. Patent No. 6,027,889); and NASBA™ RNA transcription-free amplification (see U.S. Patent No. 6,025,134).

Injectable composition: A pharmaceutically acceptable fluid composition including at least one active ingredient. The active ingredient is usually dissolved or suspended in a physiologically acceptable carrier, and the composition can additionally include minor amounts of one or more non- toxic auxiliary substances, such as emulsifying agents, preservatives, and pH buffering agents and the like. Such injectable compositions that are useful for use with the provided nucleotides and proteins are conventional; appropriate formulations are well known in the art.

Isolated: An "isolated" biological component (such as a nucleic acid molecule, protein or organelle) has been substantially separated or purified away from other biological components in the cell ofthe organism in which the component naturally occurs, i.e., other chromosomal and extra- chromosomal DNA and RNA, proteins and organelles. Nucleic acids and proteins that have been "isolated" include nucleic acids and proteins purified by standard purification methods. The term also embraces nucleic acids and proteins prepared by recombinant expression in a host cell as well as chemically synthesized nucleic acids.

MATER protein: A cytosol-localized protein (SEQ ID NO: 24) of approximately 125 to approximately 135 kDa (estimated molecular weight based on gel mobility) that is essential for female fertility. The acronym stands for Maternal Antigen That Embryos Require. Mater is a single- copy gene found on human Chromosome 19. Zygotes that arise from Mater null (Mater^'1") oocytes do not progress beyond the two-cell stage. Thus, Mater represents a novel maternal effect gene that is required for embryonic survival and early development in mammals.

Proteins can be identified as MATER proteins by comparing their activity and other physical characteristics to a prototypical MATER protein, such as the human or murine MATER protein. MATER protein biological activity can be described in terms ofthe ability of a protein to complement (substantially replace the lost function in) a Mater null mutant. The ability of a protein to complement a Mater mutant may be readily determined by introducing the gene encoding the protein into a Mater mutant animal system (such as the Mater null mice described herein) using standard methods. Ifthe encoded protein has MATER protein biological activity, this will be manifested as a proportion ofthe transgenic progeny animals having a relatively wild-type phenotype for those characteristics linked to the Mater mutant (e.g., infertility of Mater null females due to failure of Mater null oocytes to progress beyond the two-cell stage).

Other MATER protein physical characteristics that can be examined when evaluating a hypothetical MATER protein include the molecular weight ofthe protein (approximately 125-135 kDa, see Example 1, though this value may vary somewhat from species to species), the subcellular localization ofthe protein (human MATER is expressed in oocytes (Example 4)), and is predicted to be specifically expressed in the cytoplasm and excluded from the nucleus, as occurs in mice (see Example 1), and the temporal and spatial regulation ofthe mRNA (Mater transcript is produced in the maturing oocyte, see Examples 1 and 3). Antibodies that recognize one MATER protein (e.g., a murine MATER) may recognize a MATER protein from another species (e.g. , human MATER) (see Example 4); thus, hypothetical MATER proteins can be further examined and identified based on recognition by anti-MATER antibodies produced against MATER proteins from other species. The identity ofthe human MATER protein can therefore be confirmed for instance by immunological identification using an antibody raised against the murine MATER protein, or an epitope of that protein.

Nucleotide: "Nucleotide" includes, but is not limited to, a monomer that includes a base linked to a sugar, such as a pyrimidine, purine or synthetic analogs thereof, or a base linked to an amino acid, as in a peptide nucleic acid (PNA). A nucleotide is one monomer in a polynucleotide. A nucleotide sequence refers to the sequence of bases in a polynucleotide.

Oligonucleotide: An oligonucleotide is a plurality of joined nucleotides joined by native phosphodiester bonds, between about 6 and about 300 nucleotides in length. An oligonucleotide analog refers to moieties that function similarly to ohgonucleotides but have non-naturally occurring portions. For example, oligonucleotide analogs can contain non-naturally occurring portions, such as altered sugar moieties or inter-sugar linkages, such as a phosphorothioate oligodeoxynucleotide. Functional analogs of naturally occurring polynucleotides can bind to RNA or DNA, and include peptide nucleic acid (PNA) molecules.

Particular ohgonucleotides and oligonucleotide analogs can include linear sequences up to about 200 nucleotides in length, for example a sequence (such as DNA or RNA) that is at least 6 bases, for example at least 8, 10, 15, 20, 25, 30, 35, 40, 45, 50, 100 or even 200 bases long, or from about 6 to about 50 bases, for example about 10-25 bases, such as 12, 15, 20, or 23 bases.

Operably linked: A first nucleic acid sequence is operably linked with a second nucleic acid sequence when the first nucleic acid sequence is placed in a functional relationship with the second nucleic acid sequence. For instance, a promoter is operably linked to a coding sequence ifthe promoter affects the transcription or expression ofthe coding sequence. Generally, operably linked DNA sequences are contiguous and, where necessary to join two protein-coding regions, in the same reading frame.

Open reading frame: A series of nucleotide triplets (codons) coding for amino acids without any internal termination codons. These sequences are usually translatable into a peptide. Ortholog: Two nucleic acid or amino acid sequences are orthologs of each other if they share a common ancestral sequence and diverged when a species carrying that ancestral sequence split into two species. Orthologous sequences are also homologous sequences. Parenteral: Administered outside ofthe intestine, e.g., not via the alimentary tract. Generally, parenteral formulations are those that will be administered through any possible mode except ingestion. This term especially refers to injections, whether administered intravenously, intrathecally, intramuscularly, intraperitoneally, or subcutaneously, and various surface applications including intranasal, intradermal, and topical application, for instance.

Peptide Nucleic Acid (PNA): An oligonucleotide analog with a backbone comprised of monomers coupled by amide (peptide) bonds, such as amino acid monomers joined by peptide bonds.

Pharmaceutically acceptable carriers: The pharmaceutically acceptable carriers useful with the compositions provided herein are conventional. Martin, Remington 's Pharmaceutical Sciences, published by Mack Publishing Co., Easton, PA, 19th Edition, 1995, describes compositions and formulations suitable for pharmaceutical delivery ofthe nucleotides and proteins herein disclosed.

In general, the nature ofthe carrier will depend on the particular mode of administration being employed. For instance, parenteral formulations usually comprise injectable fluids that include pharmaceutically and physiologically acceptable fluids such as water, physiological saline, balanced salt solutions, aqueous dextrose, glycerol or the like as a vehicle. For solid compositions (e.g., powder, pill, tablet, or capsule forms), conventional non-toxic solid carriers can include, for example, pharmaceutical grades of mannitol, lactose, starch, or magnesium stearate. In addition to biologically-neutral carriers, pharmaceutical compositions to be administered can contain minor amounts of non-toxic auxiliary substances, such as wetting or emulsifying agents, preservatives, and pH buffering agents and the like, for example sodium acetate or sorbitan monolaurate.

Polymorphism: Variant in a sequence of a gene. Polymorphisms can be those variations (nucleotide sequence differences) that, while having a different nucleotide sequence, produce functionally equivalent gene products, such as those variations generally found between individuals, different ethnic groups, geographic locations. The term polymorphism also encompasses variations that produce gene products with altered function, i.e., variants in the gene sequence that lead to gene products that are not functionally equivalent. This term also encompasses variations that produce no gene product, an inactive gene product, or increased gene product. The term polymorphism may be used interchangeably with allele or mutation, unless context clearly dictates otherwise. Polymorphisms can be referred to, for instance, by the nucleotide position at which the variation exists, by the change in amino acid sequence caused by the nucleotide variation, or by a change in some other characteristic ofthe nucleic acid molecule that is linked to the variation (e.g., an alteration of a secondary structure such as a stem-loop, or an alteration ofthe binding affinity ofthe nucleic acid for associated molecules, such as polymerases, RNases, and so forth). Probes and primers: Nucleic acid probes and primers can be readily prepared based on the nucleic acid molecules provided as indicators of disease or disease progression. It is also appropriate to generate probes and primers based on fragments or portions of these nucleic acid molecules. Also appropriate are probes and primers specific for the reverse complement of these sequences, as well as probes and primers to 5' or 3' regions.

A probe comprises an isolated nucleic acid attached to a detectable label or other reporter molecule. Typical labels include radioactive isotopes, enzyme substrates, co-factors, ligands, chemiluminescent or fluorescent agents, haptens, and enzymes. Methods for labeling and guidance in the choice of labels appropriate for various purposes are discussed, e.g., in Sambrook et al. (In Molecular Cloning: A Laboratory Manual, CSHL, New York, 1989) and Ausubel et al. (In Current Protocols in Molecular Biology, John Wiley & Sons, New York, 1998).

Primers are short nucleic acid molecules, for instance DNA ohgonucleotides 10 nucleotides or more in length. Longer DNA ohgonucleotides may be about 15, 20, 25, 30 or 50 nucleotides or more in length. Primers can be annealed to a complementary target DNA strand by nucleic acid hybridization to form a hybrid between the primer and the target DNA strand, and then the primer extended along the target DNA strand by a DNA polymerase enzyme. Primer pairs can be used for amplification of a nucleic acid sequence, e.g., by the polymerase chain reaction (PCR) or other in vitro nucleic-acid amplification methods known in the art.

Methods for preparing and using nucleic acid probes and primers are described, for example, in Sambrook et al. (In Molecular Cloning: A Laboratory Manual, CSHL, New York, 1989), Ausubel et al. (ed.) (In Current Protocols in Molecular Biology, John Wiley & Sons, New York, 1998), and Innis et al. (PCR Protocols, A Guide to Methods and Applications, Academic Press, Inc., San Diego, CA, 1990). Amplification primer pairs (for instance, for use with polymerase chain reaction amplification) can be derived from a known sequence such as the Mater sequences described herein, for example, by using computer programs intended for that purpose such as Primer (Version 0.5, © 1991, Whitehead Institute for Biomedical Research, Cambridge, MA).

One of ordinary skill in the art will appreciate that the specificity of a particular probe or primer increases with its length. Thus, for example, a primer comprising 30 consecutive nucleotides of a MATER protein encoding nucleotide will anneal to a target sequence, such as another homolog ofthe designated MATER protein, with a higher specificity than a corresponding primer of only 15 nucleotides. Thus, in order to obtain greater specificity, probes and primers can be selected that comprise at least 20, 23, 25, 30, 35, 40, 45, 50 or more consecutive nucleotides of a MATER protein- encoding nucleotide sequences.

Also provided are isolated nucleic acid molecules that comprise specified lengths ofthe disclosed Mater nucleotide sequences. Such molecules may comprise at least 10, 15, 20, 23, 25, 30, 35, 40, 45 or 50 or more consecutive nucleotides of these sequences or more, and may be obtained from any region ofthe disclosed sequences (e.g., a Mater nucleic acid may be apportioned into halves or quarters based on sequence length, and isolated nucleic acid molecules may be derived from the first or second halves ofthe molecules, or any ofthe four quarters, etc.). A Mater cDNA or other encoding sequence also can be divided into smaller regions, e.g. about eighths, sixteenths, twentieths, fiftieths and so forth, with similar effect. Another mode of division is to select the 5' (upstream) and/or 3' (downstream) region associated with a Mater gene.

Nucleic acid molecules may be selected that comprise at least 10, 15, 20, 25, 30, 35, 40, 50 or 100 or more consecutive nucleotides of any of these or other portions of a human Mater nucleic acid molecule, such as those disclosed herein, and associated flanking regions. Thus, representative nucleic acid molecules might comprise at least 10 consecutive nucleotides ofthe human cDNA fragments show in SEQ ID NOs: 1 and 2, or the full human MATER cDNA shown in SEQ ID NO: 24.

Protein: A biological molecule expressed by a gene or recombinant or synthetic coding sequence and comprised of amino acids.

Purified: The term "purified" does not require absolute purity; rather, it is intended as a relative term. Thus, for example, a purified protein preparation is one in which the protein referred to is more pure than the protein in its natural environment within a cell or within a production reaction chamber (as appropriate). Recombinant: A recombinant nucleic acid is one that has a sequence that is not naturally occurring or has a sequence that is made by an artificial combination of two otherwise separated segments of sequence. This artificial combination can be accomplished by chemical synthesis or, more commonly, by the artificial manipulation of isolated segments of nucleic acids, e.g., by genetic engineering techniques. Sequence identity: The similarity between two nucleic acid sequences, or two amino acid sequences, is expressed in terms ofthe similarity between the sequences, otherwise referred to as sequence identity. Sequence identity is frequently measured in terms of percentage identity (or similarity or homology); the higher the percentage, the more similar the two sequences are. Homologs or orthologs of human MATER protein, and the corresponding cDNA or gene sequence, will possess a relatively high degree of sequence identity when aligned using standard methods. This homology will be more significant when the orthologous proteins or genes or cDNAs are derived from species that are more closely related (e.g., human and chimpanzee sequences), compared to species more distantly related (e.g., human and C. elegans sequences).

Methods of alignment of sequences for comparison are well known in the art. Various programs and alignment algorithms are described in: Smith & Waterman Adv. Appl. Math. 2: 482, 1981; Needleman & Wunsch J Mol. Biol. 48: 443, 1970; Pearson & Lipman Proc. Natl. Acad. Sci. USA 85: 2444, 1988; Higgins & Sharp Gene, 73: 237-244, 1988; Higgins & Sharp CABIOS 5: 151- 153, 1989; Corpet et al. Nuc. Acids Res. 16, 10881-90, 1988; Huang et al. Computer Appls. in the Biosciences 8, 155-65, 1992; and Pearson et al. Meth. Mol. Bio. 24, 307-31, 1994. Altschul etal. (J. Mol. Biol. 215:403-410, 1990), presents a detailed consideration of sequence alignment methods and homology calculations.

The NCBI Basic Local Alignment Search Tool (BLAST) (Altschul et al. J. Mol. Biol. 215:403-410, 1990) is available from several sources, including the National Center for Biotechnology Information (NCBI, Bethesda, MD) and on the Internet, for use in connection with the sequence analysis programs blastp, blastn, blastx, tblastn and tblastx. By way of example, for comparisons of amino acid sequences of greater than about 30 amino acids, the Blast 2 sequences function is employed using the default BLOSUM62 matrix set to default parameters, (gap existence cost of 11, and a per residue gap cost of 1). When aligning short peptides (fewer than around 30 amino acids), the alignment should be performed using the Blast 2 sequences function, employing the PAM30 matrix set to default parameters (open gap 9, extension gap 1 penalties).

An alternative indication that two nucleic acid molecules are closely related is that the two molecules hybridize to each other under stringent conditions. Stringent conditions are sequence- dependent and are different under different environmental parameters. Generally, stringent conditions are selected to be about 5° C to 20° C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. The T_m is the temperature (under defined ionic strength and pH) at which 50% ofthe target sequence remains hybridized to a perfectly matched probe or complementary strand. Conditions for nucleic acid hybridization and calculation of stringencies can be found in Sambrook et al. (In Molecular Cloning: A Laboratory Manual, CSHL, New York, 1989) and Tijssen (Laboratory Techniques in Biochemistry and Molecular Biology- Hybridization with Nucleic Acid Probes Part I, Chapter 2, Elsevier, New York, 1993). Nucleic acid molecules that hybridize under stringent conditions to a human MATER protein-encoding sequence will typically hybridize to a probe based on either an entire human MATER protein-encoding sequence or selected portions ofthe encoding sequence under wash conditions of 2x SSC at 50° C. Nucleic acid sequences that do not show a high degree of identity may nevertheless encode similar amino acid sequences, due to the degeneracy ofthe genetic code. It is understood that changes in nucleic acid sequence can be made using this degeneracy to produce multiple nucleic acid molecules that all encode substantially the same protein. Specific binding agent: An agent that binds substantially only to a defined target. Thus a protein-specific binding agent binds substantially only the specified protein. By way of example, as used herein, the term "MATER-protein specific binding agent" includes anti-MATER protein antibodies (and functional fragments thereof) and other agents (such as soluble receptors) that bind substantially only to the MATER protein. Anti-MATER protein antibodies may be produced using standard procedures described in a number of texts, including Harlow and Lane (Antibodies, A Laboratory Manual, CSHL, New York, 1988). The determination that a particular agent binds substantially only to the specified protein may readily be made by using or adapting routine procedures. One suitable in vitro assay makes use ofthe Western blotting procedure (described in many standard texts, including Harlow and Lane (Antibodies, A Laboratory Manual, CSHL, New York, 1988)). Western blotting may be used to determine that a given protein binding agent, such as an anti- MATER protein monoclonal antibody, binds substantially only to the MATER protein. Shorter fragments of antibodies can also serve as specific binding agents. For instance, Fabs, Fvs, and single-chain Fvs (SCFvs) that bind to a specified protein would be specific binding agents. These antibody fragments are defined as follows: (1) Fab, the fragment which contains a monovalent antigen-binding fragment of an antibody molecule produced by digestion of whole antibody with the enzyme papain to yield an intact light chain and a portion of one heavy chain; (2) Fab', the fragment of an antibody molecule obtained by treating whole antibody with pepsin, followed by reduction, to yield an intact light chain and a portion ofthe heavy chain; two Fab' fragments are obtained per antibody molecule; (3) (Fab')2, the fragment ofthe antibody obtained by treating whole antibody with the enzyme pepsin without subsequent reduction; (4) F(ab')2, a dimer of two Fab' fragments held together by two disulfide bonds; (5) Fv, a genetically engineered fragment containing the variable region ofthe light chain and the variable region ofthe heavy chain expressed as two chains; and (6) single chain antibody ("SCA"), a genetically engineered molecule containing the variable region ofthe light chain, the variable region of the heavy chain, linked by a suitable polypeptide linker as a genetically fused single chain molecule. Methods of making these fragments are routine.

Subject: Living multi-cellular vertebrate organisms, a category that includes both human and non-human mammals.

Target sequence: "Target sequence" is a portion of ssDNA, dsDNA or RNA that, upon hybridization to a therapeutically effective oligonucleotide or oligonucleotide analog, results in the inhibition of expression. For example, hybridization of therapeutically effectively oligonucleotide to a Mater target sequence results in inhibition of MATER expression. Either an antisense or a sense molecule can be used to target a portion of dsDNA, since both will interfere with the expression of that portion ofthe dsDNA. The antisense molecule can bind to the plus strand, and the sense molecule can bind to the minus strand. Thus, target sequences can be ssDNA, dsDNA, and RNA. Transformed: A transformed cell is a cell into which has been introduced a nucleic acid molecule by molecular biology techniques. As used herein, the tenn transformation encompasses all techniques by which a nucleic acid molecule might be introduced into such a cell, including transfection with viral vectors, transformation with plasmid vectors, and introduction of naked DNA by electroporation, lipofection, and particle gun acceleration. Vector: A nucleic acid molecule as introduced into a host cell, thereby producing a transformed host cell. A vector may include nucleic acid sequences that permit it to replicate in a host cell, such as an origin of replication. A vector may also include one or more selectable marker genes and other genetic elements known in the art.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The singular terms "a," "an," and "the" include plural referents unless context clearly indicates otherwise. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing ofthe present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are. incorporated by reference in their entirety. In case of conflict, the present specification, including explanations of terms, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

III. Brief Overview of Several Embodiments

Described herein is the human MATER protein, an approximately 135 kDa (predicted estimated molecular weight) cytoplasmic protein expressed in mammalian oocytes that is required for female fertility. Zygotes that arise from a MATER null oocyte do not progress beyond the two-cell stage.

One described embodiment is an isolated variant MATER protein comprising an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 24, and comprising at least one variant amino acid in a recognized MATER protein domain. Another embodiment is an isolated variant MATER protein comprising an amino acid sequence that has at least 90%) sequence identity to SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 24, and comprising at least one variant amino acid in a region outside of a recognized MATER protein domain. Yet another embodiment is an isolated variant MATER protein comprising an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 24, and comprising at least one variant amino acid in a recognized MATER protein domain and at least one variant amino acid outside of a recognized MATER protein domain.

Specific variant MATER proteins have a sequence that comprises at least one variant amino acid in a Leucine-rich repeat, or in the PAAD-DAPIN domain, or in the aldo_ket_red domain, or in the ATP-/GTP-binding domain, or in any two or more of these recognized domains. Also provided are isolated variant MATER proteins, wherein the sequence ofthe protein comprises at least one variant amino acid outside of a recognized MATER protein domain.

Other embodiments are isolated nucleic acid molecule encoding one of these proteins, recombinant nucleic acid molecule comprising a promoter sequence operably linked to such a nucleic acid molecule, and cell transformed with one of those recombinant nucleic acid molecules. Another embodiment is a method of detecting in a subject a biological condition associated with an abnormal MATER nucleic acid, which method involves reacting at least one MATER nucleic acid in a clinical sample from the subject with a reagent comprising a 477iR-specifιc nucleic acid binding agent to form a MATER-.Αgsnt complex, and detecting the complex. In specific examples of this method, the MATER-specific nucleic acid binding agent is a MATER oligonucleotide, for instance one that comprises at least 10 contiguous nucleotides of a human MATER intron sequence. By way of example, certain ofthe MATER ohgonucleotides include a sequence as shown in any one of SEQ ID NOs: 25 through 38. Another embodiment provides a method of detecting in a subject a biological condition associated with an abnormal MATER nucleic acid, which further involves quantifying the MATER.-agent complex and comparing the quantity ofthe MATER-.agent complex with the quantity of an equivalent MATER-.agent complex formed in a reference sample (e.g., one taken from a reference subject, such as a subject known to have or not to have the biological condition).

In certain specific examples of these methods, the clinical sample comprises a reproductive cell.

Representative examples of provided methods of detecting in a subject a biological condition associated with an abnormal MATER nucleic acid further comprising in vitro amplifying a MATER nucleic acid prior to detecting the abnormal Mαter nucleic acid. The in vitro amplification can be carried out in vitro using at least one oligonucleotide primer derived from a MATER protein encoding sequence. Specific examples ofthe oligonucleotide primer include a sequence of an intron-exon boundary of human MATER, for instance as shown in any on of SEQ ID NOs: 25 through 38. Other embodiments are oligonucleotide primers used in any of these methods. In certain examples ofthe described methods, the MATER-.agent complex is detected by nucleotide hybridization. The agent, used to form the MATER-.agent complex in some ofthe described methods, is a labeled nucleotide probe. Representative examples of such probes include a sequence as shown in any one of SEQ ID NOs: 25 through 38. Other embodiments are labeled nucleotide probe used in any of these methods. A further embodiment is a kit for detection of a genetic mutation in a sample of nucleic acid.

One such kit includes a first container containing an oligonucleotide capable of specifically hybridizing with a MATER nucleic acid, which oligonucleotide has a sequence comprising at least 10 contiguous nucleotides from an human MATER intron; and a second container containing a fluorescent labeled nucleic acid probe that is fully complementary to the oligonucleotide. In examples of these kits, the fluorescent labeled nucleic acid probe has a length of between 5 and 500 nucleotides. Specific examples of kits provided herein include an oligonucleotide that includes a sequence as shown in any one of SEQ ID NOs: 25 through 38.

IV. Human MA TER Protein and Nucleic Acid Sequences Embodiments provide MATER proteins and Mater nucleic acid molecules, including cDNA sequences. The prototypical Mater sequences are the murine and human sequences, and the use of these sequences to produce transgenic animals having increased or decreased levels of MATER protein is provided, as are diagnostic methods to detect defects or alterations in Mater expression or MATER protein production. The full-length cDNA for human Mater (SEQ ID NO: 23) is determined to be 3900 base pairs long (which is somewhat longer the mouse Mater cDNA, GenBank Accession # NM_011860.1 , SEQ ID NO: 5) and its ORF encodes a protein of 1200 amino acids, having a predicted molecular weight of approximately 135.2 kDa and a predicted pi of about 6.08. The human Mater cDNA comprises the sequences shown in SEQ ID NO: 1 and SEQ ID NO: 3, which are thought to be aligned approximately with the seventh and eleventh through fifteenth exons ofthe mouse Mater sequence, respectively. The human Mater mRNA is expressed in oocytes, as shown by in situ hybridization experiments (Example 3). Human MATER protein (SEQ ID NO: 24) has been specifically localized by immunofluorescence to human oocytes (Example 4). The ATG initiation codon ofthe Mater cDNA lies within the context ofthe ANNATG motif associated with vertebrate initiator codon (Kozak, 1991, J. Biol. Chem. 266:19867). The human MATER protein comprises the deduced protein sequences (SEQ ID NOs: 2 and 4) corresponding to the herein described human Mater cDNA sequences (SEQ ID NOs: 1 and 3, respectively). These protein fragments show low but significant sequence homology with the OP1/MATER protein from mouse (GenBank # NP_035990, SEQ ID NO: 6). In particular, SEQ ID NO: 2 is 54% identical to residues 257-638 of murine MATER (SEQ ID NO: 6), while SEQ ID NO: 4 is 64% identical to residues 854-1111 of murine MATER. The overall human MATER protein sequence (SEQ ID NO: 24) shares approximately 50% similarity with the murine MATER protein sequence (SEQ ID NO: 6); the relevant sequence alignment is shown in FIG. 9. The depicted comparison between the human and murine MATER proteins was conducted using the BLAST 2 Sequences Program with parameter conditions of Expect 10 and Filter closed (NIH-NCBI).

Certain regions ofthe human Mater cDNA have been identified through the sequencing of human HTGSs, though no function or identity had been previously assigned to those sequences. See, for instance, GenBank accession numbers: AC024580 (Gl = 7705010, published May 4, 2000); AC012107 (Gl = 6088020, published October 20, 1999, and updated as Gl = 7329252, published March 28, 2000); and AC023887 (Gl = 7631054, published April 21, 2000). These fragmentary and overlapping human sequences are up to 207 nucleotides in length and are scattered throughout the murine Mater cDNA sequence. The human ESTs individually share up to 89% identity with the murine Mater cDNA over short, discontinuous regions. Ohgonucleotides according to the current disclosure may be chosen to avoid these ESTs. Overall, the first 75 nucleotides of human Mater (1- 75) and the corresponding deduced amino acids (1-25) were determined by comparison to published human genomic DNA sequences, while the remainder ofthe Mater sequence was determined by direct cloning and sequencing of human ovarian cDNAs.

Using the sequence ofthe cloned human MATER cDNA to blast the human hgts database (which is accessible on-line), a single genomic clone (AC01740) with a full DNA sequence was determined to harbor an entire gene locus of human MATER.

Mater is a single-copy maternal effect gene, the protein product of which is required for early embryonic survival. Although eggs lacking MATER protein can be fertilized, morphologic signs of deterioration are observed as early as the 2-cell stage beyond which the mutant embryos do not progress. The human MATER protein with 1200 amino acids is predicted to have similar molecular domains to those seen in the murine MATER protein (1111 amino acids). As described by Tong et l. (Mamm. Genome 11:281-287, 2000), the mouse MATER protein contains a five-fold hydrophilic repeat (26-27 amino acid) near its N-terminus, a short leucine-zipper and a fourteen-fold leucine-rich repeat (28-29 amino acid) near its carboxyl terminus (based on a comparison to the murine MATER protein, as described in Tong et al, Mamm. Genome 11, 281-287, 2000). The hydrophilic repeat has low homology with a cytoskeletonal protein (neurofilament), raising the possibility that this region mediates interactions that anchor MATER in the cytoplasm. The presence ofthe leucine-rich domain, as well as a short leucine zipper, both motifs known to mediate protein- protein interactions (Kajava, J. Mol. Biol. 277, 519-527, 1998; Buchanan & Gay, Prog. Biophys. Mol. Biol. 65, 1-44, 1996), suggests that MATER may affect embryonic progression through intermediate factor(s), one or more of which binds directly to MATER in the cytoplasm.

Using antisera against a mouse MATER peptide (residues 1093 through 1111 of murine MATER, SEQ ID NO: 6), the inventors have demonstrated that human MATER protein is present in oocytes in human ovary sections (Example 4). Likewise, using mouse-derived nucleotide sense and antisense probes for in situ hybridization experiments, it has been demonstrated that human oocytes express Mater mRNA (Example 3).

Murine MATER has been further characterized herein as to its function in mammalian embryogenesis. This protein is a "maternal" protein, generated in the oocyte prior to fertilization, and therefore encoded for by a maternal gene. MATER is unusual in that it persists (as measured by protein level) into the late blastocyst stage of embryonic development. Functional MATER is required for female fertility; zygotes that arise from Mater null oocytes do not progress beyond the two-cell stage; this is true regardless of what Mater genotype male produced the sperm. The protein is cytoplasmic, with definite exclusion from the nucleus.

The following examples are provided to illustrate certain particular features and/or embodiments. These examples should not be construed to limit the invention to the particular features or embodiments described.

EXAMPLE 1 Characterization ofthe Murine MATER protein

This example provides several methods for examining MATER proteins and nucleic acids in a mammalian system; the murine system is used.

Methods:

Isolation of oocytes, eggs and embryos. All experiments were conducted in compliance with the guidelines ofthe Animal Care and Use Committee under an approved animal study protocol. Resting, growing and fully grown oocytes were dissected from 1, 10 and 21 day old (d/o) mouse ovaries, respectively, and eggs were isolated from gonadotrophin stimulated female mice (Tong et al, J. Biol. Chem. 270, 849-853, 1995; Rankin et al, Development 125, 2415-2424, 1998). Embryos were obtained by mating gonadotrophin stimulated females with males and 1-cell zygotes, 2-cell embryos, morulae and blastocysts were flushed from the oviducts at 1, 2, 3 and 4 days, respectively, counting the morning after hCG administration as day 1. Embryos were either incubated in Ml 6 media (37 C, 5% C0 ), fixed in 1% paraformaldehyde for subsequent confocal microscopy, or frozen at -80 C for RNA and protein analyses. Ovaries were fixed (10% formalin) and embedded in paraffin prior to sectioning and staining with hematoxylin and eosin (American Histolabs, Gaithersburg, Maryland).

Detection of transcripts and proteins. Mater, ZP3, β-actin, cyclophilin and 28S-rRNA transcripts were detected by RNase protection assays as previously described (Tong & Nelson, Endocrinology 140, 3720-3726, 1999;Tong et al, J. Biol. Chem. 270, 849-853, 1995) using an RPA II kit (Ambion, Austin, TX).

Rabbits were immunized with a KLH conjugated MATER peptide (amino acids 1093-1111) to obtain a monospecific antisera. After incubating immunoblots with MATER antisera (1 : 1000, two hours, 20 °C), antibody binding was detected by ECL using a HRP-conjugated goat anti-rabbit antibody (Amersham Pharmacia Biotech, Piscataway, NJ). Fixed oocytes, eggs or embryos were incubated with MATER antisera (1 :8000, 4 °C, 16 hours) and imaged by laser-scanning confocal microscopy (LSM 5; Zeiss, Thornwood, NY) using Cy⁵-conjugated goat anti-rabbit IgG (1:200).

Protein synthesis and immunoblotting. Embryos were incubated in 100 μl of Ml 6 medium containing L-³⁵S-methionine (0.5 Ci/ml, Amersham) at 37 °C for 4 hours. At the end of radiolabeling, 1-cell and 2-cell embryos were harvested at 26 hours and 48 hours post-hCG/mating, respectively. After washing with 1% BSA in 10 mM Tris-HCl (pH 7.4), ImM EDTA and 140 mM NaCl, ten embryos were dissolved directly in 10 μl of sample buffer (1) directly or (2) after treatment with 2% Triton X-100/0.3 M KC1 to extract the TRC and p35 (Conover et al, Dev. Biol. 144, 392-404, 1991). After SDS-PAGE and fluorography as previously reported (Tong et al, J. Biol. Chem. 270, 849-853, 1995), radioactive incorporation was determined by a phosphoimager and ImageQuant software (Molecular Dynamics, Amersham Pharmacia Biotech, Piscataway, NJ).

Production of Mater null mice. Constructs use to produce Mater null mice are shown in FIG. 2. To construct a targeting vector in pPNT (Tybulewicz ef al, Cell 65, 1153-1163, 1991), a 1.5 kbp SacI-EcoRI DNA fragment containing the first two exons of murine Mater (Tong et al., Mamm. Genome 11, 281-287, 2000) was inserted between the PGK-Neo and PGK-TK cassettes and a 2 kbp EcoRI-BamHI fragment containing exons 3 and 4 was inserted upstream ofthe PCK-Neo. After linearization and electroporation into embryonic stem (ES) cells (Redmond et al, Nat. Genet. 20, 344-351, 1998), the presence ofthe mutant allele in chemically selected clones was detected as 3.5 kbp and 4.0 kbp Xbal fragments with 5' and 3' (or Neo) probes, respectively. Both the 5' and 3' probes detected the normal allele as a 9.4 kbp Xbal fragment. C57BL/6 blastocysts were injected with 8-12 ES cells derived from five independently selected clones. Two cell lines that gave rise to coat-color chimeric animals were mated with C57BL/6 females and transmitted the Mater mutation through the germ line. The absence of Mater transcripts in homozygous null females was confirmed by northern blot analysis as described previously (Tong & Nelson, Endocrinology 140, 3720-3726, 1999).

Reproductive performance. Vaginal smears were obtained daily to examine four or more estrus cycles of 6-10 week-old female mice (Rugh, The Mouse: Its Reproduction and Development 210 Oxford University Press, Oxford, 1991). Mating behavior was evaluated by the presence of a vaginal plug on the morning after mating with fertile males.

Zygotic gene transcription assays. A 5-10 pi aliquot of 100 mM BrUTP, 140 mM KC1 and 2mM Pipes, pH 7.4 was microinjected into the cytoplasm of embryos isolated 28 hours (1-cell embryos) and 48 hours (2-cell embryos) following hCG and mating, essentially as previously described (Bouniol et al, Exp. Cell Res. 218, 57-62, 1995). After incubation in M16 medium in 5% C0₂ for one hour at 37 °C, the injected embryos were fixed in 1% paraformaldehyde.

BrUTP incorporation into RNA was assayed with a mouse anti-BrdU monoclonal antibody (Sigma, 1:1000, 16 hours, 4 °C). After washing in PBS containing 3% BSA, a FITC-conjugated goat anti-mouse IgG antibody was used to image the embryos by confocal microscopy; fluorescence was recorded in arbitrary units. This assay primarily reflects de novo RNA polymerase II activity, because poor penetration ofthe monoclonal antibody into nucleoli prevents measurement of BrUTP incorporation into ribosomal RNA (Wansink, et al., J. Cell Biol 122, 283-293, 1993).

Results Mater transcript and protein expression

Mater mRNA was first detected as oocytes entered into their growth phase (30-50 μm) (FIG. 1 A). Mater transcripts were most abundant in fully-grown oocytes (75-85 μm) and, like many oocyte transcripts (Bachvarova, in Developmental Biology: Oogenesis 1, 453-524, Plenum Press, New York, 1985; Epifano et al, Development 121, 1947-1956, 1995), were degraded during meiotic maturation and subsequent ovulation. Mater mRNA was not detected in the early embryo (FIG. 1 A), although its presence in EST databases derived from early embryos suggest that some transcripts may have escaped destruction.

The initial accumulation of MATER protein during oogenesis was similar to that observed for Mater transcripts (compare FIG. 1A with FIG. IB). However, unlike the virtual disappearance of the transcripts, the MATER protein persisted during preimplantation development until the late blastocyst stage (FIG. IB). Using confocal microscopy, MATER protein was located in the cytoplasm of growing oocytes and noticeably excluded from the nucleus. MATER was more concentrated in the cortical region of oocytes and early embryos (one- and two-cell stages), and this peripheral pattern persisted later in preimplantation development with a higher concentration of MATER in the outer trophectodermal cells compared to the inner cell mass of blastocysts (FIG. IC). Mater null animals

To determine the function of MATER protein, Mater null mouse lines were generated, in which homozygous females did not express either Mater transcripts or protein. Mater null mice were born in the expected Mendelian ratios with equal sex distribution. No phenotypic abnormalities were observed from birth through adulthood.

Ovaries and uteri from Mater null mice appeared morphologically normal (FIG. 6). Mater null ovaries had a normal complement of primordial follicles and all stages of follicular development were normally represented; corpora lutea, indicating past spontaneous ovulation, were also present (FIG. 5). Ova lacking Mater were fertilized normally in vivo (FIG. 1). The number and morphology of zygotes and 2-cell embryos from the Mater null females were similar to those from normal females (FIG. 5). However, by 3 or 4 days after mating the embryos from Mater null females still remained at the 2-cell stage or had begun to degenerate (compare FIG. 3D and 3G with 3E and 3H). Thus, fertilization is normal in Mater null females and the resulting zygotes can progress through the first cleavage, but subsequent development is arrested at the 2-cell stage. This indicates that an arrest of early embryogenesis accounts for the infertility in Mater null females.

Two mouse lines with a Mater null mutation (T54 and T85) were generated (FIG. 2A and 2B) and neither Mater transcript (FIG. 2C) nor protein (FIG. 2D) were detected in homozygous null females.

Reproductive phenotype of Mater null animals Matings of heterozygous Mater null parents produced average sized litters (8.5 ± 1.9) with normal Mendelian and equal sex distribution ofthe null allele in progeny. Homozygous null animals appeared normal and males were as fertile as normal littermates. However, homozygous null females produced no litters, even after five months of mating (Table 1) which implicated Mater as a maternal effect gene.

Table 1: Fertility and Mendelian allele ratio of mice with Mater mutation

* n, the num er of mating pairs, n.d., not done, n.a., not applicable. Sexually mature Mater null females exhibited regular 5.67 ± 0.22 days estrus cycles, similar to those observed in normal females (5.40 ± 0.23 days). Mating occurred normally, as demonstrated by the appearance of vaginal plugs in about 70% of either Mater null or normal females, after exposure to fertile males. The Mater null ovary had a normal complement of primordial follicles, with all stages of follicular development as well as corpora lutea indicating past spontaneous ovulations (FIG. 5A and 5B). In addition, the Mater null females ovulated normally after stimulation with exogenous gonadotropins. However, homozygous null females produced no litters even after five months of mating with normal males, while the homozygous null males and heterozygous females had normal fertility. The mean number (± s.e.m.) of ovulated eggs recovered from null (27.6 ± 4.5, n=14) was not statistically different (p > 0.05) from that obtained from normal (27.7 ± 4.3, n=12) females (FIG. 5D). Morphologically, the two groups of eggs were indistinguishable from one another (FIG. 5C).

Fertilization and embryo development in Mater null animals To evaluate in vivo fertilization and early development, embryos were isolated from normal and Mater null females at 1, 2, 3 and 4 days after mating with normal males. Fertilization occurred in eggs lacking MATER, as demonstrated by the presence of two pronuclei (arrows, FIG. 3A and 3B). The number and morphology of 1-cell zygotes and 2-cell embryos obtained in vivo from the Mater null females were similar to those observed from normal females, although the 2-cell mutant embryos appeared less healthy, displaying cytoplasmic granulations (FIG. 3A-3F). Embryos obtained from the Mater null female mice 3 or 4 days after mating still remained at the 2-cell stage (or had begun to degenerate), while embryos from normal mice had progressed to the morula or blastocyst stages, respectively

An equal percentage (-70%) of fertilized 1-cell zygotes derived from Mater null and normal females progressed in vitro to the 2-cell stage. However, after four days in culture, embryos lacking MATER remained arrested at the 2-cell stage (most were degenerating) (FIG. 3K); embryos from the normal females developed to the blastocyst stage in the same time (FIG. 3 J). Thus, although fertilization appears normal and zygotes without MATER can progress through the first cleavage, subsequent embryonic development is arrested at the 2-cell stage.

Characterization of transcription in Mater null animals The early arrest phenotype seen in Mater null mice is reminiscent ofthe 2-cell block that occurs after exposure of mouse embryos to α-amanitin (Schultz, Bioessays 15:531-538, 1993; Flach et al, EMBO J. 1, 681-686, 1982), a mushroom toxin that binds to RNA polymerase II and prevents elongation of transcription beyond dinucleotides (Vaisius & Wieland, Biochemistry 21, 3097-3101, 1982). Normal embryonic transcription is first detected late in the 1-cell zygote at a level that is -20% that ofthe 2-cell embryo (Aoki et al, Dev. Biol. 181, 296-307, 1997), but these nascent transcripts are poorly, if at all, translated into protein (Nothias et al, EMBO J. 15, 5715-5725, 1996). To assess de novo RNA polymerase II activity ofthe early Mater null embryo, bromo-UTP (BrUTP) incorporation was assayed with a monoclonal antibody specific to BrUTP using laser- scanning confocal microscopy, essentially as described previously (Bouniol et al, Exp. Cell Res. 218, 57-62, 1995) (FIG. 4A-4G). Morphologically there was a dramatic difference in the amount of BrUTP detected in the nuclei of normal embryos 28 hours (FIG. 4B) and 48 hours (FIG. 4D) after mating compared to those lacking MATER (FIG. 4A and 4C). BrUTP incorporation in embryos without MATER was marginally greater than that of uninjected controls (FIG. 4E and 4F) and de novo transcription was less than 5% that observed in normal 2-cell embryos (FIG. 4G).

Characterization of TRC in Mater null animals

During meiotic maturation and ovulation, more than 50% of maternal RNA is lost in normal mice (Bachvarova, in Developmental Biology: Oogenesis 1, 453-524, Plenum Press, New York, 1985). This degradative process also occurs in females lacking MATER with loss of ribosomal RNA (FIG. 4H) and at least some mRNAs (β-actin, ZP3, cyclophilin, and GAPDH). Normally, the subsequent activation ofthe embryonic genome at the 2-cell stage is preceded by the transient transcription and translation of a subset of gene products, some of which form the distinctive Transcription Related Complex (TRC) (Bolton et al, J. Embryol. Exp. Morphol 79:139-163, 1984; Ηothias et al, EMBO J. 15:5715-5725, 1996; Conover et al., Dev. Biol. 144:392-404, 1991). This complex, first observed in the early 2-cell embryo, is not formed in the presence of α-amanitin. To assay for the TRC, 1- and 2-cell embryos derived from Mater null females mated with normal males were incubated with ³⁵S-methionine. No global differences in the amount of ³⁵S- methionine incorporation or in protein profiles were noted in comparing normal 1-cell zygotes and those lacking MATER (FIG. 41, left panel). Although TRC complexes were detected in embryos without MATER at the 2-cell stage (FIG. 41, middle and right panels), they appeared somewhat less abundant (-60% of normal), which could reflect abnormal protein synthesis in the embryos lacking MATER. This indicates that MATER is not absolutely critical for initiation of all transcription- translation machinery in early embryos.

Although this striking decrease in transcription could reflect generalized morbidity ofthe embryo, a similar decrease in 1-cell zygotes, which appear quite healthy, as well as absence of an equally dramatic decrease in de novo protein synthesis (FIG. 41) suggest that the depressed levels of transcription are due either directly, or indirectly to the absence of MATER.

Function(s) of mammalian MATER

The homology ofthe leucine-rich repeat domain of mammalian MATER protein with porcine ribonuclease inhibitor implies that MATER may act as an inhibitor of cytoplasmic RNase. If this is true, RNA in embryos lacking MATER might be subject to extensive degradation, perhaps an accentuation ofthe normal turnover of RNA that occurs during the maternal-to-zygotic transition in the early embryo. The degradation of RNAs, including those that encode proteins required for transcription, could account for the phenotype observed in Mater null embryos. This seems unlikely; however, because in the absence of Mater mutant embryos incorporate ³⁵S-methionine into proteins and no major difference in protein profiles are observed in comparison with normal embryos. These results indicate that sufficient mRNA, tRNA and ribosomal RNA are present in the mutant embryos to support protein biosynthesis, although it does not preclude the degradation of a targeted subset of RNA required for early embryonic survival.

Transcription-dependent protein synthesis occurs in two phases in the 2-cell embryo, an early minor activation (2-4 hours after the first mitotic division) that is characterized by appearance of the TRC proteins, and a later major activation that occurs in G₂ (8-10 hours after the first mitotic division). The later activation initiates developmental programs required for progression beyond the 2-cell stage (Schultz, Bioessays 15, 531-538, 1993). The observed decrease in embryonic transcription in embryos lacking MATER could result from deterioration of transcription machinery, decreased access to genomic DNA or abnormalities in the chromatin template within the nucleus. However, the presence ofthe TRC proteins in Mater null animals indicates that MATER is not critical for the early phase of transcription-dependent protein synthesis in 2-cell embryos. It further suggests that the transcription machinery, and its ability to access nuclear DNA, is largely intact in Mater null embryos (as it appears to be in Mater null oocytes, which are also transcriptionally active).

The TRC proteins resulting from the early burst of transcription are associated with the nucleus (Schultz, Bioessays 15, 531-538, 1993). These proteins could relieve the late transcriptional repression required for progression beyond the 2-cell stage via mechanisms involving MATER.

Additionally, it has been reported that mouse oocytes and 1-cell embryos lack coactivator(s) required for enhancer-dependent transcription in 2-cell embryos (Lawinger et al, J. Biol. Chem. 21 A, 8002- 8011, 1999). The nature of these coactivators is unknown; they may arise by modification of preexisting maternal proteins or from early transcription-dependent protein synthesis in the 2-cell embryo. Such process may involve cytoplasmic MATER. Alternatively, MATER might be involved in one or more interactions that are required for cell cycle progression beyond the 2-cell stage. Identification and characterization of proteins that interact with MATER will provide insights into the role of this maternal protein in promoting embryonic survival and early development.

Mater is the first maternal effect gene demonstrated to play a critical role in early mammalian development and the observed sterile phenotype raises the possibility that the absence of a similar molecule could be a cause of human infertility.

EXAMPLE 2: Identification ofthe Human MATER cDNA The human MATER cDNA was identified based on its homology to the mouse MATER cDNA sequence by searching the collection of human High Throughput Genomic (HTG) Sequences, maintained by NCBI and the National Library of Medicine. Using the isolated and discontinuous nucleotide fragments identified in this search, the inventors devised oligonucleotide primers pairs (for instance, SEQ ID NOs: 7 and 8, 19 and 20, and 21 and 22) that where used to amplify MATER sequence from purified human ovarian DNA or mRNA/cDNA, and to isolate a partial cDNA clone from a human cDNA library, using standard techniques.

The sequence of two long fragments ofthe human MATER cDNA is shown in SEQ ID NO: 1 and 3. Together, these two long cDNA fragments constitute 2213 nucleotides ofthe complete human MATER cDNA.

The deduced human MATER protein portions encoded cDNA fragment 1 (SEQ ID NO: 1) and fragment 2 (SEQ ID NO: 3) are shown in SEQ ID NOs: 2 and 4, respectively.

Using these sequences, the remainder ofthe human MATER cDNA has been identified; the sequence ofthe full length cDNA is shown in SEQ ID NO: 23. Overall, the first 75 nucleotides of human MATER (1-75) and the corresponding deduced amino acids (1-25) were determined by comparison to published human genomic DNA sequences, while the remainder ofthe Mater sequence was determined by direct cloning and sequencing of human ovarian cDNAs. The complete sequence ofthe human MATER protein is shown in SEQ ID NO: 24.

EXAMPLE 3: Detection of Mater RNA in human ovarian total RNA This example provides one method of detecting MATER RNA in human ovarian total RNA. Human ovarian total RNA from a 19-year-old woman was obtained under an IRB-approved protocol (McGill University, Montreal, Canada) and the use of it was approved by the NIH office of Human Subjects Research. Ovarian RNA from a 26 year old woman was purchased from BioChain Inc. (Hayward, CA, USA). Separation of human ovarian total RNA (10 μg) was performed by 1% agarose/formaldehyde gel electrophoresis. The RNA was transferred onto a nitrocellulose membrane to prepare a Northern blot. Human Mater cDNA (-0.5 kb) was labeled with α-[³²P]dCTP (3000 Ci/mmol; ICN, Costa Mesa, CA, USA) using Ready-To-Go DNA dCTP Beads (Pharmacia, Inc., Piscataway, NJ, USA). Blots were hybridized (68 °C, 2 hours) with ³²P-labeled cDNA probe (-0.5 kb) in QuikHyb solution (Stratagene, La Jolla, CA, USA). After washing the blots with a solution of O.lxstandard saline citrate/0.1% sodium dodecyl sulfate (SDS) at 65°C, the hybridization signals were detected by autoradiography. This Northern blot hybridization revealed the presence of a single -4.2 kb MATER transcript in the human ovarian total RNA (Figure 8A). The human MATER transcripts appeared to be larger than the mouse MATER transcript (3.75 kb). The relatively greater abundance of MATER mRNA detected in the ovarian RNA from a 19 year old woman (lane 1), compared with the RNA from a 26 year old woman (lane 2), may reflect the relatively greater number of oocytes present in ovaries from younger women. EXAMPLE 4: In vitro amplification via PCR for human MATER transcripts

This example provides one method of detecting MATER transcripts in RNA samples from different tissues. To determine whether expression of human MATER transcripts is ovarian-specific, in vitro amplification (in this case, PCR amplification) reactions were carried out using cDNA that were derived from human RNA of 24 different tissues (Human Rapid-Scan Gene Expression Panel).

Human ovarian cDNA (Invitrogene; Origene), human ovarian cDNA library (Clonetech), and the Human Rapid-Scan Gene Expression Panel (Origene), were used as templates for PCR reactions, essentially following the manufacturer's instructions. Taq DNA polymerase (Life

Technologies, Rockville, MD, USA) was used to amplify DNA products expected within 1.5 kbp at a condition ofthe PCR reaction (95°C, 1 minute; 60°C, 1 minute; 72V, 2 minutes; and 72V, 10 minutes for further extension). Platinum PCR SuperMix (Life Technologies) was used for amplification of DNA products (2-5 kb) with prolonging extension time at 72 °C in every cycle, according to the product's manual. The PCR products were electrophoresed on agarose gels containing 0.1% ethidium bromide and subcloned into a TA cloning vector (Invitrogen) for DNA sequencing.

As a control, as shown in figure 8B, the PCR products (640 base pairs) of human β-actin were amplified in all cDNA derived from 25 tissues and human ovary (lanes 1-25) using the human - β-actin-specific primers (5'-GCATGGGTCAGAAGGAT-373'-GTCCAGTAGTGGTAACC'5'; SEQ ID NOs: 39 and 40). In contrast, using human MATER -specific primers, (5'-

TGACTTCTGATTGCTGTGAGG-3'/3'~CTACTGGCCATGACCACCTT-5'; SEQ ID NOs: 41 and 42), the PCR products (280 base pairs) were only generated in the cDNA synthesized from the ovarian RNA (Figure 8C, lanes 17 and 25).

EXAMPLE 5:

Southern Blot Hybridization OF Human MATER cDNA to PCR-amplified MATER Transcripts

This example provides one method of confirming the identity ofthe DNA amplified using 477ii?-specific PCR primers.

Ovarian-specific expression ofthe human MATER transcripts was further evidenced by a Southern blot hybridization ofthe human MATER primer-amplified PCR reactions probed with radiolabeled MATER cDNA (Figure 8D).

PCR products obtained in Example 5 were separated by 2% agarose gel electrophoresis and transferred onto a nitrocellulose membrane to prepare a Southern blot. Human MATER cDNA (-0.5 kbp) was labeled with α-[³²P]dCTP (3000 Ci/mmol; ICN, Costa Mesa, CA, USA) using Ready-To-Go DNA dCTP Beads (Pharmacia, Inc., Piscataway, NJ, USA). Blots were hybridized (68°C, 2 hours) with ³²P-labeled cDNA probe (-0.5 kbp) in QuikHyb solution (Stratagene, La Jolla, CA, USA). After washing the blots with a solution of O.lx standard saline citrate/0.1% sodium dodecyl sulfate (SDS) at 65°C, the hybridization signals were detected by autoradiography. EXAMPLE 6: Localization of Human MATER transcript

This example provides one method for detecting expression ofthe human MATER transcript. To produce in situ hybridization probes, human genomic DNA was amplified using the following primers:

5'-primer: 5'- TTTCACATGAACATCCTTCTCC-3' (SEQ ID NO: 7); 3'-primer: 5'-AGTGCTGGAGGCAGAAGGAAG-3' (SEQ ID NO: 8).

The resultant amplified nucleic acid molecule product size was 496 bp, and is predicted to fall within exon 7 ofthe human MATER gene, based on comparison to the murine MATER exon-intron map. This fragment was subcloned into pBluscript vector as a DNA template. Both ³⁵S-UTP-labeled sense and antisense sequences were synthesized by in vitro transcription using T3 and T7 RNA polymerases. In situ hybridization was carried out essentially as described previously (Tong & Nelson,

Endocrinology 140:3720-3726, 1999). Briefly, the probes were prepared by alkaline hydrolysis and hybridized with frozen human ovarian sections at 60 °C for 24 hours. After dipping in Kodak NTB-2 emulsion, the slides were exposed to film for 2-3 days and the autoradiographs developed.

The frozen human ovarian sections were hybridized with the radio-labeled sense (FIG. 9A and 9C) and antisense (B and D) probes. The slides were stained with hematoxylin and eosin. For each probe, bright-field (A and B) and dark-field (C and D) images are displayed. MATER transcript was localized to oocytes.

EXAMPLE 7: In situ hybridization with MATER probes

This example provides another method for detecting expression ofthe human MATER transcript.

Both [³⁵S]UTP-labeled sense and antisense probes were synthesized by in vitro transcription using human MATER cDNA as templates and T /T₇ RNA polymerase (Ambion, Inc., Austin, TX, USA). Short probes (-300 nt) of alkaline hydrolysis were hybridized at 60°C for 24 hours with frozen sections (8μ) of human ovary obtained from the National Disease Research Institute (NDRJ; Philadelphia, PA, USA). After dipping in Kodak NTB-2 emulsion, the slides were exposed for 1 week on an X-ray film for developing in Kodak D-19 and Kodak Fixer. The slides were finally stained with hematoxylin and eosin, and examined by microscopy under both light and dark fields. In situ hybridization revealed the oocyte-specific expression of human MATER transcripts.

As shown in Figure 8E, the hybridization signals using synthetic human MATER antisense probe were present within the oocytes but not in the follicular cells. The control MATER sense probes did not hybridize with oocytes or the ovarian cells (Figure 8F). Thus, the oocyte-restricted expression ofthe human MATER gene appears similar to the expression pattern ofthe mouse MATER gene.

EXAMPLE 8: Localization of Human MATER Protein

This example provides one method for examining the localization of human MATER protein.

In situ localization was carried out essentially as described for murine samples (see, Tong and Nelson, Endocrinology 140:3720-3726, 1999). Briefly, rabbit polyclonal antibody to a murine MATER C-terminal peptide (residues 1093 through 1111 of SEQ ID NO: 6) was prepared as described in Example 18C. Frozen human ovarian sections were incubated with this antiserum (1 :200), and FITC-conjugated goat anti-rabbit IgG antiserum was used as the secondary antibody to detect human MATER protein in the oocyte (FIG. 10A). FIG. 10B shows the phase contrast images corresponding to the samples in FIG. 10A.

EXAMPLE 9: Expression of Human MATER Protein in Oocytes This example provides one method for examining the localization of human MATER protein. After blocking with 10% normal goat sera in phosphate-buffered saline (PBS), frozen sections (8μ) of human ovary (NDRI) were incubated at 25°C for 2 hours with rabbit antisera (1 :200) specific to mouse Mater peptide. The slides were washed with 0.05% Tween-20 in PBS, and incubated with a goat anti-rabbit IgG antibody conjugated with fluorescein isothiocyanate (1:300; Sigma Chemical Co., St. Louis, MO, USA). After washing and applying with glycerol-glass cover, the slides were examined under fluorescent microscopy.

No fluorescence was observed in somatic cells in the ovarian section other than the oocytes (Figure 11 A and 1 IB). Normal rabbit sera did not stain the oocytes.

EXAMPLE 10: Immunoblotting to Determine the Size of Native MATER Protein

This example provides one method for determining the size ofthe native Mater protein.

Human immature oocytes were supplied for this study by a clinical IVF program (Rockvill Reproductive Science Center, Rockville, MD, USA) after obtaining consent and approval ofthe NIH office of Human Subjects Research. For immunoblotting, human oocyte proteins were separated on a 3-8% Tris-acetate SDS-polyacrylamide gel electrophoresis. After transferring onto a nitrocellulose membrane, 5% dry milk in PBS was used to block non-specific binding in the blot. Subsequently, the blot was incubated with rabbit anti-Mater peptide antibody (1:1000) for 2 hours at 25°C. After the blot was washed, the primary antibody-bound proteins were detected by a goat anti-rabbit IgG antibody (1:1000) labeled with horseradish peroxidase using an enhanced chemiluminescence system according to the manufacturer's instructions (Amersham/Pharmacia, Inc., Piscatoway, NJ, USA).

As shown in Figure 1 IC, human MATER protein (-134 kDa) was detected in the preparation ofthe human oocyte proteins using anti-mouse MATER antibody. It was noted that there were two close signals of MATER proteins present in the immunoblotting. This might reflect a post- translational modification of human MATER protein or the possibility of an alternative initiation site in the synthesis ofthe polypeptide chain.

EXAMPLE 11: Methods of Making Human MA TER cDNA

The original means by which the Mater cDNA was identified and obtained is described above. With the provision ofthe sequence ofthe large portions ofthe MATER protein (SEQ ID NOs: 2 and 4) and encoding nucleic acid molecules (SEQ ID NOs: 1 and 3), nucleotide amplification (such as polymerase chain reaction (PCR)) now may be utilized in a simple method for producing the MATER cU A.

Total RNA is extracted from human cells by any one of a variety of methods well known to those of ordinary skill in the art. Sambrook et al. (In Molecular Cloning: A Laboratory Manual, CSHL, New York, 1989) and Ausubel et al. (In Current Protocols in Molecular Biology, Greene Publ. Assoc. and Wiley-Intersciences, 1992) provide descriptions of methods for RNA isolation. Because MATER is expressed in oocytes, human cell lines derived from oocytes or ovaries can be used as a source of such RNA. The extracted RNA is then used as a template for performing reverse transcription-polymerase chain reaction (RT-PCR) amplification of cDNA. Methods and conditions for RT-PCR are described in Kawasaki et al, (In PCR Protocols, A Guide to Methods and Applications, Innis et al. (eds.), 21-27, Academic Press, Inc., San Diego, California, 1990). The selection of amplification primers will be made according to the portion(s) ofthe cDNA that is to be amplified. Primers may be chosen to amplify a segment of a cDNA or the entire cDNA molecule. Variations in amplification conditions may be required to accommodate primers and amplicons of differing lengths and composition; such considerations are well known in the art and are discussed for instance in Innis et al. (PCR Protocols, A Guide to Methods and Applications, Academic Press, Inc., San Diego, CA, 1990). By way of example, the majority ofthe coding portion ofthe human MATER cDNA molecule (approximately 2.8 kb) may be amplified using the following combination of primers:

5'-primer (part of exon 7): 5'-CAGGAATTGGGAAATCGGCTCTCTAG-3' (SEQ ID NO.: 9); 3'-primer (end of exon 15):

5'-CCAAATGCTTTGTGTTTATTTAATTCC-3' (SEQ ID NO.: 10). One of ordinary skill can use the full length human MATER cDNA sequence provided herein (SEQ ID NO: 23) to devise primers that could be used to amplify the entire cDNA using known techniques.

The following set of four primers can be used to amplify -950 bp at the 5'-end of human MATER cDNA:

The 5'-primers are the universal adapter primers (ADP) for 5'-RACE-PCR as follows (OriGene Technologies, Inc, Rockville, MD):

ADPl: 5'-CGGAATTCGTCACTCAGCG-3' (SEQ ID NO.: 11)

ADP2: 5'-AGCGCGTGAATCAGATCG-3' (SEQ ID NO.: 12)

The 3 '-primers are gene-specific primers (GSP) for human MATER cDNA within exon 7, as follows: GSPl: 5'-ATTCCCTGGTAGAGTCCACCTTGC-3' (SEQ ID NO.: 13) GSP2: 5'-ACAGCACGATCCTTCTGGCTAGAG-3' (SEQ ID NO.: 14).

GSPl is used as a primer for reverse transcription of human ovarian RNA to synthesize cDNA. The reverse transcribed cDNAs are then adapted at the 5 '-end by ADPl primer, then amplified for the first round of PCR using ADPl and GSPl primers. The second round of PCR amplification is followed using PCR products from the first round as template, and ADP2 and GSP2 as primers.

Fragment 1 ofthe human MATER cDNA (SEQ ID NO: 1) may be amplified using the following combination of primers:

5'-primer: 5'-CAAGCTCCGGTGACGGAGATCAT-3' (SEQ ID NO.: 15); 3'-primer: 5'-AGCTGGAGGCAGAAGGAAGATG-3' (SEQ ID NO.: 16).

The 3 '-terminal region ofthe human MATER cDNA molecule (also referred to herein as human MATER cDNA fragment 2, SEQ ID NO: 3) may be amplified using the following combination of primers:

5'-primer: 5'-TCTGGCCTCAGCCCTCGTCAGCTTGAC-3' (SEQ ID NO.: 17); 3'-primer: 5'-CCAAATGCTTTGTGTTTATTTAATTCC-3' (SEQ ID NO.: 18).

These primers are illustrative only; one skilled in the art will appreciate that many different primers may be derived from the provided cDNA sequence in order to amplify particular the indicated regions MATER cDNA, as well as to complete the sequence ofthe human MATER cDNA. Re-sequencing of PCR products obtained by these amplification procedures is recommended; this will facilitate confirmation ofthe amplified sequence and will also provide information on natural variation on this sequence in different populations or species. Ohgonucleotides derived from the provided MATER sequences provided may be used in such sequencing methods. Orthologs of human MATER can be cloned in a similar manner, where the starting material consists of cells taken from a non-human species. Orthologs will generally share at least 80% sequence homology with the disclosed human MATER cDNA. Where the non-human species is more closely related to humans, the sequence homology will in general be greater. Closely related orthologous MATER molecules may share at least 82%, at least 85%, at least 90%, at least 91%, at least 93%), at least 95%, or at least 98% sequence homology with the disclosed human sequences. Ohgonucleotides derived from the human MATER cDNA sequence (SEQ ID NO: 23), or fragments thereof (such as SEQ ID NOs: 1 and 3), are encompassed within the scope ofthe present disclosure. Preferably, such oligonucleotide primers will comprise a sequence of at least 23 consecutive nucleotides ofthe Mater nucleic acid sequence. To enhance amplification specificity, oligonucleotide primers comprising at least 25, 30, 35, 40, 45 or 50 consecutive nucleotides of these sequences may also be used. These primers for instance may be obtained from any region ofthe disclosed sequences. By way of example, the human Mater cDNA, ORF and gene sequences may be apportioned into about halves or quarters based on sequence length, and the isolated nucleic acid molecules (e.g., ohgonucleotides) may be derived from the first or second halves ofthe molecules, or any ofthe four quarters. The murine MATER cDNA, shown in SEQ ID NO: 5, can be used to illustrate this. The human MATER cDNA is 3447 nucleotides in length and so may be hypothetically divided into about halves (nucleotides 1-1723 and 1724-3447) or about quarters (nucleotides 1-862, 863-1723, 1724-2586 and 2587-3447). The human cDNA can be likewise apportioned, or can be described in terms ofthe fragments presented herein (e.g., fragment 1, corresponding to around about nucleotide 1700 through around about 1950 of murine Mater; and fragment 2 ofthe human Mater cDNA, corresponding to the 3 '-terminal region of murine Mater cDNA, around about nucleotide 2500 through the polyA tail ofthe murine Mater cDNA).

Also encompassed herein are ohgonucleotides from within the human MATER gene, for use in the methods described herein. These include, but are not limited to, ohgonucleotides that span or include the sequence spanning an intron-exon boundary ofthe human MATER gene (see Table 2).

Nucleic acid molecules may be selected that comprise at least 15, 20, 23, 25, 30, 35, 40, 50 or 100 consecutive nucleotides of any of these or other portions ofthe human MATER cDNA. Thus, representative nucleic acid molecules might comprise at least 15 consecutive nucleotides ofthe human MATER cDNA (SEQ ID NO: 23), or fragment 1 or fragment 2 ofthe disclosed human Mater coding sequence (SEQ ID NOs: 1 and 3, respectively).

EXAMPLE 12: Isolation of Human MATER cDNA This example provides another method of making human MA TER cDNA.

Human MATER oligonucleotide primers were designed to amplify, using PCR, nucleic acids from human ovarian cDNA and a library produced therefrom (see Example 4). Using the PCR- cloned cDNA as the probes for in situ hybridization, human oocytes were confirmed to express the corresponding transcripts (see Example 5). DNA sequencing revealed that these PCR products are human MATER cDNA homologous to the mouse Mater cDNA.

The PCR products were generated to span -98% ofthe coding region from nt 76 to nt 3596. The 3' non-coding regions were determined by sequencing cDNA clones isolated from the human ovarian cDNA library using the PCR-cloned cDNA as probe. The first 75 nt at the 5' end were derived from a predicted cDNA ofthe genomic clone AC011470 (http://www.ensemble.org). The non-coding region present at the 5' end ofthe Mater cDNA remains undetermined. Assuming the presence of a poly(A) tail (-150-200 nt), assembly ofthe determined human MATER cDNA sequence, its poly(A) tail and 5' undetermined non-coding region correspond in size with the MATER transcripts detected in the Northern hybridization (4.2 kb; Example 3). This suggests that the cloned cDNA sequence represents a full-length coding region.

As shown in Figure 12, the open reading frame (nt 1 to nt 3600) ofthe cloned cDNA (3885 nt) encodes a protein composed of 1200 amino acids. The entire polypeptide chain is predicted to have a 1 6.08 and a molecular mass of 134,235.76 DA, which corresponded to the size ofthe human oocyte protein recognized by the antisera specific to murine Mater peptide. Expression of a recombinant protein further confirmed the cloned cDNA to encode the human MATER protein.

The initiator code (ATG) encoding the first methionine in the open reading frame (ORF) was in a context (ATCATGAAG), the consensus sequence recognized for initiation in vertebrates. The stop codon (TGA) was followed by 282 nt ofthe 3' non-coding sequence. A signal sequence (AATAAA) for polyadenylation was located just 18 nt upstream ofthe poly(A) tail. None ofthe known genes and proteins deposited in GenBank was found to have significant homologies to the human MATER gene or MATER protein. However, the carboxy 1-terminal sequence ofthe human MATER peptide had a -32% homology with human and porcine ribonuclease inhibitors (457 residues). Thus, human MATER contains a structural domain ofthe ribonuclease inhibitor-like Leucine-rich repeats at the carboxy terminus, as found in mouse. The human and mouse MATER cDNAs share 67% homology, while their deduced polypeptide chains share 53% amino acid identity.

EXAMPLE 13: Analysis of Human MATER Polypeptide Chain This example provides one method of analyzing the structural domains ofthe human

MATER protein.

Computational analyses of DNA sequences and deduced peptide sequences were conducted using software available at the National Center for Biotechnology Information at NIH (http://www.nbci.nlm.nih.gov), the EMBL-EBI (http://www.ensemble.org), the Sanger Center (http://www.sanger.ac.uk) and the ExPASy Molecular Biology Server ofthe Swiss Institute for Bioinformatics (http://www.expasy.ch).

A computational analysis was carried out to search for the structural features ofthe human MATER protein. When the entire polypeptide chain of human MATER was compared with itself, the DotPlot analysis revealed the presence of repeat structural motifs at amino- and carboxy 1-termini. These repeats were evidenced by a series of parallel but offset lines in these regions (Figure 13 A), as also seen in the mouse MATER protein. A comparison between the polypeptide chains of human and mouse MATER indicated the similarities and homologies of their structural motifs and compositions (Figure 13B).

A hydrophobicity plot (Figure 14A) indicated that there were hydrophilic regions present at amino- (32-304 residues) and carboxyl-termini (1184-1200 residues). No structural motifs were found for either peptide signals of protein secretion, or for transmembrane domains. The peptide chain had some notable motifs, as indicated in Figure 14B. A signal for nuclear localization was present at the residue 517-534 (NLS_BP), which was not detected in the mouse MATER. There appears to be no recognizable peptide domains for DNA binding. Further experimental evidence is required to determine ifthe human MATER is translocated into nuclei.

Human MATER protein had six Leucine-rich repeats at the carboxyl-terminus, and a PAAD_DAPIN domain (52-148 residues), both of which are recognized as the structural basis for protein-protein interactions. Their presence implies that human MATER interacts with other oocyte proteins to perform its function. In addition, human MATER protein had an aldo ketjred domain (71-87) at the amino-terminus, and an ATPJGTP-binding domain (P-loop) at residue 286-293. The aldo ketjred is a domain present in protein with oxireductase activity, and the P-loop is the typical domain for binding to ATP/GTP in participating in an intracellular signal transduction.

EXAMPLE 14: Genomic Organization of the Human MATER Locus This example provides a method of determining the size and numbers of exons and introns in the MATER genomic sequence. Using the sequence of cloned human MATER cDNA, one human genomic clone (GenBank

Accession Number AC011470) was determined to carry an entire gene locus of human MATER.

Using the sequence ofthe cloned human MATER cDNA to blast the human hgts database (which is accessible on-line, for instance via the NCBI web site), a single genomic clone (AC011470) that had been fully sequenced was determined to harbor an entire gene locus of human MATER. To determine the number and size ofthe exons and introns, the sequence ofthe cloned cDNA was compared with the sequence ofthe AC011470clone isolated from human chromosome 19 (Fig. 15 A and B). The AG-exon-GT rule was applied to determine a splicing site between exon and introns (Table 2).

The 15 exons and 14 introns were identified to span a -63 kb region of DNA. The exons ranging in size from 56 to 1597 bp were intervened by 14 introns ranging from 760 to 9327 bp (Table 2). In addition, there were 282 nucleotides ofthe 3' non-coding region within exon 15, and undetermined nucleotides ofthe 5' non-coding region within exon 1. The sequences switching between each exon and introns were confirmed to meet with the bordering consensus sequences (AG- -GT) for splice sites ofthe exon-intron. Thus, the human MATER locus spanned a -63 kb DNA and was composed ofthe 15 exons and 14 introns.

Table 2. Exon and intron and splice-junction sequences of human MATER

Exon Splice-junction sequences Intron

No. Position Length Exon No. Length

1 1-62 >62 1 GTCGACAGCC 1 3928

2 63-442 380 TCTTTTAAAG 2 GTAAGCGAGA 2 4692

3 443-508 66 TGTCTTCCAG 3 GTTAGAGGGG 3 6982

4 509-565 57 TCTTTTGCAG 4 GTGAATGAAA 4 3543

5 566-623 58 TCTTTTGCAG 5 GTGTGGAAAA 5 976

6 624-679 56 TCTTTTGCAG 6 GTGAGGAAAA 6 6487

7 680-2276 1597 CTGACTCCAG 7 GTGAGTACCC 7 4118

8 2277-2447 171 CCTGACATAG 8 GTAAGGCTGC 8 760

9 2448-2615 168 CCCATTGCAG 9 GTACGTCTCT 9 4313

10 2616-2786 171 GTCTCTGCAG 10 GTGAGTGCCA 10 2726

11 2787-2957 171 CTTCTTGCAG 11 GTGAGTCTGG 11 9327

12 2958-3128 171 TGCCCCACAG 12 GTGAGTTTCC 12 3036

13 3129-3299 171 CCTCTTGCAG 13 GTAACTTCCT 13 4606

14 3300-3470 171 GACCCTGCAG 14 GTAAGTCGCC 14 2988

15 3471-3885 415 GCCTCCCCAG 15 GTTGTCAAGT

Human MATER and mouse Mater loci had identical numbers of exons and introns, while the human locus was longer than the mouse locus (32 kbp). This mainly reflects the larger size ofthe human introns. The counterpart exons were comparable. Both had a large exon 7 spanning -50% of the coding sequences, and the counterparts of exons 8-14 had an identical number of nucleotides. However, the first six exons (1-6) in the human locus ranged in size from 56-380 nt, while their counterparts in the mouse locus had 48-78 nt. A larger exon 2 (380 nt) in the human MATER locus made the human MATER cDNA coding region (3600 nt) slightly longer than the mouse cDNA coding region (3333 nt). All of these structural similarities and variations reflect conservation and evolution between mouse Mater and human MA TER loci.

EXAMPLE 15: Cloning of the Mater Genomic Sequence (or Gene)

The Mater cDNA sequence and fragments thereof described above does not contain introns, upstream transcriptional promoter or regulatory regions or downstream transcriptional regulatory regions ofthe MATER gene. It is possible that some mutations in the MATER gene that may lead to defects in embryo development, infertility, or reduced fertility are not included in the cDNA but rather are located in other regions ofthe MATER gene. Mutations located outside ofthe open reading frame that encodes the MATER protein are not likely to affect the functional activity ofthe protein, but rather are likely to result in altered levels ofthe protein in the cell. For example, mutations in the promoter region ofthe MATER gene may prevent transcription ofthe gene and therefore lead to the complete absence ofthe MATER protein in the cell.

Additionally, mutations within intron sequences in the genomic gene may also prevent expression ofthe MATER protein. Following transcription of a gene containing introns, the intron sequences are removed from the RNA molecule in a process termed splicing prior to translation ofthe RNA molecule which results in production ofthe encoded protein. When the RNA molecule is spliced to remove the introns, the cellular enzymes that perform the splicing function recognize sequences around the intron/exon border and in this manner recognize the appropriate splice sites. If there is a mutation within the sequence ofthe intron close to the junction ofthe intron with an exon, the enzymes may not recognize the junction and may fail to remove the intron. If this occurs, the encoded protein will likely be defective. Thus, mutations inside the intron sequences within the MATER gene (termed "splice site mutations") may also lead to defects in embryo development. However, knowledge ofthe exon structure and intronic splice site sequences of the MATER gene is required to define the molecular basis of these abnormalities. The provision herein ofthe MATER cDNA sequence and the sequence ofthe MATER gene

(as now identified within ACO 11470) enables the cloning ofthe entire MATER gene (including the promoter and other regulatory regions and the intron sequences). With this information in hand, diagnosis of a genetic predisposition to fertility defects based on DNA analysis will comprehend all possible mutagenic events at the MATER locus. The MATER gene may be isolated by one or more routine procedures, including direct sequencing of one or more BAC or PAC clones that contain the MATER sequence. Alternatively, using the infonnation provided herein, in particular the identification ofthe entire gene sequence from within GenBank Accession No. AC011470, portions ofthe MATER gene (or the entire gene) can be amplified from genomic DNA using in vitro amplification techniques. For long portions ofthe gene, or the entire gene, it may be advantageous to use techniques especially tailored to amplification of long sequences. Such techniques are known to those of ordinary skill in the art.

With the sequences of human MATER cDNA and gene in hand, primers derived from these sequences may be used in diagnostic tests (described below) to determine the presence of mutations in any part ofthe genomic MATER gene of a patient. Such primers will be ohgonucleotides comprising a fragment of sequence from the MATER gene (intron sequence, exon sequence, or a sequence spanning an intron-exon boundary) and may include at least 10 consecutive nucleotides of the MATER cDNA or gene. Specific examples of such ohgonucleotides include those comprising the sequences shown in SEQ ID NOs: 25-38. It will be appreciated that greater specificity may be achieved by using primers of greater lengths. Thus, in order to obtain enhanced specificity, the primers used may comprise 15, 17, 20, 23, 25, 30, 40 or even 50 consecutive nucleotides ofthe MATER cDNA or gene, particularly overlapping an intron-exon boundary. Furthermore, with the provision ofthe MATER intron sequence information, the analysis of a large and as yet untapped source of patient material for mutations is now possible using methods such as chemical cleavage of mismatches (Cotton et al, Proc. Natl. Acad. Sci. USA 85:4397-4401, 1985; Montandon et al, Nucleic Acids Res. 9:3347-3358, 1989) and single-strand conformational polymorphism analysis (Orita et al, Genomics 5:874-879, 1989).

Additional experiments may be performed to identify and characterize regulatory elements flanking the MATER gene. These regulatory elements may be characterized by standard techniques including deletion analyses wherein successive nucleotides of a putative regulatory region are removed and the effect ofthe deletions are studied by either transient or long-term expression analyses experiments. The identification and characterization of regulatory elements flanking the genomic MATER gene may be made by functional experimentation (deletion analyses, etc.) in mammalian cells by either transient or long-term expression analyses.

It will be apparent to one skilled in the art that either the genomic clone or the cDNA or sequences derived from these clones may be utilized in applications, including but not limited to, studies ofthe expression ofthe MATER gene, studies ofthe function ofthe MATER protein, the generation of antibodies to the MATER protein diagnosis and therapy of MATER deleted or mutated patients to prevent or treat the defects in embryo development. Descriptions of applications describing the use of MATER cDNA, or fragments thereof, are therefore intended to comprehend the use ofthe genomic MATER gene.

It will also be apparent to one skilled in the art that homologs of this gene may now be cloned from other species, such as the rat or a monkey, by standard cloning methods. Such homologs will be useful in the production of animal models of onset and development of autoimmune infertility, and early embryogenesis. In general, such orthologous MATER molecules will share at least 70% sequence identity with the human MATER nucleic acid disclosed herein; more closely related orthologous sequences will share at least 75%, at least 80%, at least 90%, at least 95%, or at least 98%) sequence identity with this sequence.

EXAMPLE 16: Mater Nucleic Acid and Amino Acid Sequence Variants With the provision of human MATER protein fragments and corresponding nucleic acid sequences herein, the creation of variants of these sequences is now enabled. Variant MATER proteins include proteins that differ in amino acid sequence from the human

MATER sequences disclosed but that share at least 65% amino acid sequence homology with the provided human MATER protein. Other variants will share at least 70%, at least 75%, at least 80%, at least 90%, at least 95%>, or at least 98% amino acid sequence homology. Manipulation ofthe nucleotide sequence of Mater using standard procedures, including for instance site-directed mutagenesis or PCR, can be used to produce such variants. The simplest modifications involve the substitution of one or more amino acids for amino acids having similar biochemical properties. These so-called conservative substitutions are likely to have minimal impact on the activity ofthe resultant protein. Table 3 shows amino acids that may be substituted for an original amino acid in a protein, and which are regarded as conservative substitutions.

Table 3

Original Residue Conservative Substitutions

Ala ser

Arg lys

Asn gin; his

Asp glu

Cys ser

Gin asn

Glu asp

Gly pro

His asn; gin

He leu; val

Leu ile; val

Lys arg; gin; glu

Met leu; ile

Phe met; leu; tyr

Ser thr

Thr ser

Trp tyr

Tyr trp; phe

Val ile; leu

More substantial changes in enzymatic function or other protein features may be obtained by selecting amino acid substitutions that are less conservative than those listed in Table 3. Such changes include changing residues that differ more significantly in their effect on maintaining polypeptide backbone structure (e.g., sheet or helical conformation) near the substitution, charge or hydrophobicity ofthe molecule at the target site, or bulk of a specific side chain. The following substitutions are generally expected to produce the greatest changes in protein properties: (a) a hydrophilic residue (e.g., seryl or threonyl) is substituted for (or by) a hydrophobic residue (e.g., leucyl, isoleucyl, phenylalanyl, valyl or alanyl); (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain (e.g., lysyl, arginyl, or histadyl) is substituted for (or by) an electronegative residue (e.g., glutamyl or aspartyl); or (d) a residue having a bulky side chain (e.g., phenylalanine) is substituted for (or by) one lacking a side chain (e.g., glycine).

Variant MATER encoding sequences may be produced by standard DNA mutagenesis techniques, for example, M13 primer mutagenesis. Details of these techniques are provided in Sambrook et al. (In Molecular Cloning: A Laboratory Manual, CSHL, New York, 1989), Ch. 15. By the use of such techniques, variants may be created that differ in minor ways from the human MATER sequences disclosed. DNA molecules and nucleotide sequences that are derivatives of those specifically disclosed herein, and which differ from those disclosed by the deletion, addition, or substitution of nucleotides while still encoding a protein that has at least 82% sequence identity with the human MATER encoding sequence disclosed (SEQ ID NOs: 1 and 3), are comprehended by this disclosure. Also comprehended are more closely related nucleic acid molecules that share at least

80%, at least 85%, at least 90%, at least 95%, or at least 98% nucleotide sequence homology with the disclosed Mater sequences. In their most simple form, such variants may differ from the disclosed sequences by alteration ofthe coding region to fit the codon usage bias ofthe particular organism into which the molecule is to be introduced. The full length human MATER cDNA (SEQ ID NO: 23) is also encompassed in the disclosure, and as a molecule that comprises both fragment 1 (SEQ ID NO: 1) and fragment 2 (SEQ ID NO: 3), and encoding the human MATER protein with described physical characteristics and biological properties.

Alternatively, the coding region may be altered by taking advantage ofthe degeneracy ofthe genetic code to alter the coding sequence such that, while the nucleotide sequence is substantially altered, it nevertheless encodes a protein having an amino acid sequence substantially similar to the disclosed human MATER protein sequences. For example, because ofthe degeneracy ofthe genetic code, four nucleotide codon triplets - (GCT, GCG, GCC and GCA) - code for alanine. The coding sequence of any specific alanine residue within the human MATER protein, therefore, could be changed to any of these alternative codons without affecting the amino acid composition or characteristics ofthe encoded protein. Based upon the degeneracy ofthe genetic code, variant DNA molecules may be derived from the cDNA and gene sequences disclosed herein using standard DNA mutagenesis techniques as described above, or by synthesis of DNA sequences. Thus, this disclosure also encompasses nucleic acid sequences that encode a MATER protein, but which vary from the disclosed nucleic acid sequences by virtue ofthe degeneracy ofthe genetic code.

Variants ofthe MATER protein may also be defined in terms of their sequence identity with the prototype human MATER protein. As described above, human MATER proteins share at least 65%>, at least 70%, at least 75%, at least 80%, at least 90%, at least 95%, or at least 98% amino acid sequence identity with the human MATER protein (SEQ ID NO: 24) or fragments disclosed herein (such as SEQ ID NOs: 2 and 4). Nucleic acid sequences that encode such proteins/fragments readily may be determined simply by applying the genetic code to the amino acid sequence of an MATER protein or fragment, and such nucleic acid molecules may readily be produced by assembling ohgonucleotides corresponding to portions ofthe sequence.

Variant MATER proteins may contain one or more variant amino acids (amino acids different from the prototypical sequence provided herein). For instance, specific variant MATER proteins have at least two, at least three, at least 4, at least 5, at least 6, at least 8, at least 10, at least 15, at least 20, or even 25, 30, 50, 100 or more variant amino acids compared to the prototypical MATER sequence (SEQ ID NO: 24).

Particular embodiments include MATER variant proteins wherein at least one amino acid sequence variation is within a recognized protein domain or recognized functional sequence. Specific examples include variations in one ofthe Leucine-rich repeats at the carboxyl-terminus, the PAAD_DAPIN domain (52-148 residues), the aldo_ket_red domain (71-87) at the amino-terminus, or the ATP-/GTP-binding domain (P-loop) at residue 286-293. Thus, in particular examples a variant MATER protein (or nucleic acid encoding such) will include one or more residue changes in a leucine-rich repeat, or in the PAAD_DAPIN domain; such example proteins may have differential interactions with other proteins in the cell. Specific variant MATER proteins of this type are interaction-disruptive, and affect (e.g., inhibit or enhance) MATER function in the cell, and may be defective in one or more other activities ofthe MATER protein.

In other embodiments, a variant MATER protein (or nucleic acid encoding such) has one or more sequence variations in the aldo_ket_red domain. Specific examples of such variants disrupt an oxireductase activity ofthe protein, and may be defective in one or more other activities ofthe MATER protein.

In still other embodiments, a variant MATER protein (or nucleic acid encoding such) has one or more sequence variations in the ATP-/GTP-binding domain (P-loop). Examples of such variant proteins are defective in ATP and/or GTP binding and may be defective in one or more other activities ofthe MATER protein.

In some embodiments, a variation in the amino acid sequence occurs in each of two or more such defined domains.

In still other embodiments, a protein sequence variation occurs outside of these defined domains. Examples of such variants include variants occurring, for instance, in any one of residues 149 through 285. Specific examples of such variant proteins will include more than one sequence variation.

Nucleic acid molecules that are derived from the human MATER cDNA nucleic acid sequences disclosed include molecules that hybridize under stringent conditions to the disclosed prototypical MATER nucleic acid molecules, or fragments thereof. Stringent conditions are hybridization at 65° C in 6 x SSC, 5 x Denhardt's solution, 0.5% SDS and 100 μg/ml sheared salmon testes DNA, followed by 15-30 minute sequential washes in 2 x SSC, 0.5% SDS, followed by 1 x SSC, 0.5% SDS and finally 0.2 x SSC, 0.5% SDS, at 65° C.

Low stringency hybridization conditions (to detect less closely related homologs) are performed as described above but at 50°C (both hybridization and wash conditions); however, depending on the strength ofthe detected signal, the wash steps may be terminated after the first 2 x SSC wash.

Human MATER nucleic acid encoding molecules (including the cDNA shown in SEQ ID NO: 23 and fragments shown in SEQ ID NOs: 1 and 3, and nucleic acids comprising these sequences), and orthologs and homologs of these sequences, may be incorporated into transformation or expression vectors.

EXAMPLE 17: Expression of MATER Protein and Variants thereof With the provision of human MATER cDNA sequence fragments, and methods for determining and cloning the full length human MATER cDNA, the expression and purification ofthe MATER protein by standard laboratory techniques is now enabled. Purified human MATER protein may be used for functional analyses, antibody production, diagnostics, and patient therapy. Furthermore, the DNA sequence ofthe MATER cDNA can be manipulated in studies to understand the expression ofthe gene and the function of its product. Mutant forms ofthe human MATER may be isolated based upon information contained herein, and may be studied in order to detect alteration in expression patterns in terms of relative quantities, cellular localization, tissue specificity and functional properties ofthe encoded mutant MATER protein. Partial or full-length cDNA sequences, which encode for the subject protein, may be ligated into bacterial expression vectors. Methods for expressing large amounts of protein from a cloned gene introduced into Escherichia coli (E. col ) may be utilized for the purification, localization and functional analysis of proteins. For example, fusion proteins consisting of amino terminal peptides encoded by a portion ofthe E. coli lacZ or trpE gene linked to MATER proteins may be used to prepare polyclonal and monoclonal antibodies against these proteins. Thereafter, these antibodies may be used to purify proteins by immunoaffinity chromatography, in diagnostic assays to quantitate the levels of protein and to localize proteins in tissues and individual cells by immunofluorescence. Such antibodies may be specific for epitope tags, which can be added to the expression construct for identification an/or purification purposes. Intact native protein may also be produced in E. coli in large amounts for functional studies.

Methods and plasmid vectors for producing fusion proteins and intact native proteins in bacteria are described in Sambrook et al. (Sambrook et al, In Molecular Cloning: A Laboratory Manual, Ch. 17, CSHL, New York, 1989). Such fusion proteins may be made in large amounts, are easy to purify, and can be used to elicit antibody response. Native proteins can be produced in bacteria by placing a strong, regulated promoter and an efficient ribosome-binding site upstream ofthe cloned gene. If low levels of protein are produced, additional steps may be taken to increase protein production; if high levels of protein are produced, purification is relatively easy. Suitable methods are presented in Sambrook et al. (In Molecular Cloning: A Laboratory Manual, CSHL, New York, 1989) and are well known in the art. Often, proteins expressed at high levels are found in insoluble inclusion bodies. Methods for extracting proteins from these aggregates are described by Sambrook et al. (In Molecular Cloning: A Laboratory Manual, Ch. 17, CSHL, New York, 1989). Vector systems suitable for the expression of lacZ fusion genes include the pUR series of vectors (Ruther and Muller- Hill, EMBOJ. 2:1791, 1983), pEXl-3 (Stanley and Luzio, EMBOJ. 3:1429, 1984) and pMRlOO (Gray et al, Proc. Natl Acad. Sci. USA 79:6598, 1982). Vectors suitable for the production of intact native proteins include pKC30 (Shimatake and Rosenberg, Nature 292:128, 1981), pKK177-3

(Amann and Brosius, Gene 40:183, 1985) and pET-3 (Studiar and Moffatt, J. Mol. Biol. 189:113, 1986). MATER fusion proteins may be isolated from protein gels, lyophilized, ground into a powder and used as an antigen. The DNA sequence can also be transferred from its existing context to other cloning vehicles, such as other plasmids, bacteriophages, cosmids, animal viruses and yeast artificial chromosomes (YACs) (Burke et al, Science 236:806-812, 1987). These vectors may then be introduced into a variety of hosts including somatic cells, and simple or complex organisms, such as bacteria, fungi (Timberlake and Marshall, Science 244:1313-1317, 1989), invertebrates, plants, and animals (Pursel et al, Science 244:1281-1288, 1989), which cells or organisms are rendered transgenic by the introduction of the heterologous Mater cDNA.

For expression in mammalian cells, the cDNA sequence may be ligated to heterologous promoters, such as the simian virus (SV) 40 promoter in the pSV2 vector (Mulligan and Berg, Proc. Natl. Acad. Sci. USA 78:2072-2076, 1981), and introduced into cells, such as monkey COS-1 cells (Gluzman, Cell 23: 175- 182, 1981), to achieve transient or long-term expression. The stable integration ofthe chimeric gene construct may be maintained in mammalian cells by biochemical selection, such as neomycin (Southern and Berg, J. Mol. Appl. Genet. 1:327-341, 1982) and mycophenolic acid (Mulligan and Berg, Proc. Natl. Acad. Sci. USA 78:2072-2076, 1981). DNA sequences can be manipulated with standard procedures such as restriction enzyme digestion, fill-in with DNA polymerase, deletion by exonuclease, extension by terminal deoxynucleotide transferase, ligation of synthetic or cloned DNA sequences, site-directed sequence- alteration via single-stranded bacteriophage intermediate or with the use of specific ohgonucleotides in combination with nucleic acid amplification. The cDNA sequence (or portions derived from it) or a mini gene (a cDNA with an intron and its own promoter) may be introduced into eukaryotic expression vectors by conventional techniques. These vectors are designed to permit the transcription ofthe cDNA in eukaryotic cells by providing regulatory sequences that initiate and enhance the transcription ofthe cDNA and ensure its proper splicing and polyadenylation. Vectors containing the promoter and enhancer regions ofthe SV40 or long terminal repeat (LTR) ofthe Rous Sarcoma virus and polyadenylation and splicing signal from SV40 are readily available (Mulligan et al, Proc. Natl. Acad. Sci. USA 78:1078-2076, 1981; Gorman et al, Proc. Natl. Acad. Sci USA 78:6777-6781, 1982). The level of expression ofthe cDNA can be manipulated with this type of vector, either by using promoters that have different activities (for example, the baculovirus pAC373 can express cDNAs at high levels in S. frugiperda cells (Summers and Smith, In Genetically Altered Viruses and the Environment, Fields et al. (Eds.) 22:319-328, CSHL Press, Cold Spring Harbor, New York, 1985) or by using vectors that contain promoters amenable to modulation, for example, the glucocorticoid-responsive promoter from the mouse mammary tumor virus (Lee et al, Nature 294:228, 1982). The expression ofthe cDNA can be monitored in the recipient cells 24 to 72 hours after introduction (transient expression). In addition, some vectors contain selectable markers such as the gpt (Mulligan and Berg,

Proc. Natl. Acad. Sci. USA 78:2072-2076, 1981) or neo (Southern and Berg, J. Mol. Appl Genet. 1:327-341, 1982) bacterial genes. These selectable markers permit selection of rransfected cells that exhibit stable, long-term expression ofthe vectors (and therefore the cDNA). The vectors can be maintained in the cells as episomal, freely replicating entities by using regulatory elements of viruses, such as papilloma (Sarver et al, Mol. Cell Biol. 1 -.486-496, 1981) or Epstein-Barr (Sugden et al, Mol. Cell Biol. 5:410-413, 1985). Alternatively, one can also produce cell lines that have integrated the vector into genomic DNA. Both of these types of cell lines produce the gene product on a continuous basis. One can also produce cell lines can also produced that have amplified the number of copies of the vector (and therefore ofthe cDNA as well) to create cell lines that can produce high levels ofthe gene product (Alt et at, J Biol. Chem. 253:1357-1370, 1978).

The transfer of DNA into eukaryotic, in particular human or other mammalian cells, is now a conventional technique. Recombinant expression vectors can be introduced into the recipient cells as pure DNA (transfection) by, for example, precipitation with calcium phosphate (Graham and vander Eb, Virology 52:466, 1973) or strontium phosphate (Brash et al, Mol. Cell Biol. 7:2013, 1987), electroporation (Neumann et al, EMBO J 1:841, 1982), lipofection (Feigner et al., Proc. Natl. Acad. Sci USA 84:7413, 1987), DEAE dextran (McCuthan et al, J. Natl. Cancer Inst. 41:351, 1968), microinjection (Mueller et al, Cell 15:579, 1978), protoplast fusion (Schafher, Proc. Natl. Acad. Sci. USA 77:2163-2167, 1980), or pellet guns (Klein et al, Nature 327:70, 1987). Alternatively, the cDNA, or fragments thereof, can be introduced by infection with virus vectors. Systems are developed that use, for example, retroviruses (Bernstein et al, Gen. Engr'g 7:235, 1985), adenoviruses (Ahmad et al, J. Virol. 57:267, 1986), or Herpes virus (Spaete et al, Cell 30:295, 1982). Techniques of use in packaging long transcripts can be found in Kochanek et al. (Proc. Natl. Acad. Sci. USA 93:5731-5739, 1996) Parks et al. (Proc. Natl Acad. Sci. USA 93:13565-13570, 1996) and Parks and Graham (J. Virol. 71:3293-3298, 1997). MATER encoding sequences can also be delivered to target cells in vitro via non-infectious systems, for instance liposomes.

These eukaryotic expression systems can be used for studies of MATER encoding nucleic acids and mutant forms of these molecules, the MATER protein and mutant forms of this protein. Such uses include, for example, the identification of regulatory elements located in the 5' region ofthe Mater gene on genomic clones that can be isolated from human genomic DNA libraries using the information contained herein. The eukaryotic expression systems also may be used to study the function ofthe normal complete protein, specific portions ofthe protein, or of naturally occurring or artificially produced mutant proteins. Using the above techniques, expression vectors containing the MATER gene sequence or cDNA, or fragments or variants or mutants thereof, can be introduced into human cells, mammalian cells from other species or non-mammalian cells, as desired. The choice of cell is determined by the purpose ofthe treatment. For example, monkey COS cells (Gluzman, Cell 23:175-182, 1981) that produce high levels ofthe SV40 T antigen and permit the replication of vectors containing the SV40 origin of replication may be used. Similarly, Chinese hamster ovary (CHO), mouse NIH 3T3 fibroblasts or human fibroblasts or lymphoblasts (as described herein) may be used.

Embodiments described herein thus encompass recombinant vectors that comprise all or part of a MATER encoding sequence, such as the MATER gene or cDNA or variants thereof, for expression in a suitable host. The MATER DNA is operatively linked in the vector to an expression control sequence in the recombinant DNA molecule so that the MATER polypeptide can be expressed. The expression control sequence may be selected from the group consisting of sequences that control the expression of genes of prokaryotic or eukaryotic cells and their viruses and combinations thereof. The expression control sequence may be specifically selected from the group consisting ofthe lac system, the trp system, the tac system, the trc system, major operator and promoter regions of phage lambda, the control region of fd coat protein, the early and late promoters of SV40, promoters derived from polyoma, adenovirus, rerrovirus, baculovirus and simian virus, the promoter for 3-phosphoglycerate kinase, the promoters of yeast acid phosphatase, the promoter ofthe yeast alpha-mating factors and combinations thereof.

The host cell, which may be transfected with a vector, may be selected from the group consisting of E. coli, Pseudomonas, Bacillus subtilis, Bacillus stearothermophilus or other bacilli; other bacteria; yeast; fungi; insect; mouse or other animal; or plant hosts; or human tissue cells.

It is appreciated that for mutant or variant MATER DNA sequences, similar systems are employed to express and produce the mutant product.

EXAMPLE 18: Production of an Antibody to MATER Protein

Monoclonal or polyclonal antibodies may be produced to either the normal MATER protein or mutant forms of this protein. Optimally, antibodies raised against the MATER protein would specifically detect the MATER protein. That is, such antibodies would recognize and bind the MATER protein and would not substantially recognize or bind to other proteins found in human cells. Antibodies the human MATER protein may recognize MATER from other species, such as murine MATER, and vice versa. The determination that an antibody specifically detects the MATER protein is made by any one of a number of standard immunoassay methods; for instance, the Western blotting technique (Sambrook et al, In Molecular Cloning: A Laboratory Manual, CSHL, New York, 1989). To determine that a given antibody preparation (such as one produced in a mouse) specifically detects the MATER protein by Western blotting, total cellular protein is extracted from human cells (for example, lymphocytes) and elecfrophoresed on a sodium dodecyl sulfate-polyacrylamide gel. The proteins are then transferred to a membrane (for example, nitrocellulose or PVDF) by Western blotting, and the antibody preparation is incubated with the membrane. After washing the membrane to remove non-specifically bound antibodies, the presence of specifically bound antibodies is detected by the use of (by way of example) an anti-mouse antibody conjugated to an enzyme such as alkaline phosphatase. Application of an alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium results in the production of a dense blue compound by immunolocalized alkaline phosphatase. Antibodies that specifically detect the MATER protein will, by this technique, be shown to bind to the MATER protein band (which will be localized at a given position on the gel determined by its molecular weight, which is approximately 125 kDa based on gel-mobility estimation for murine MATER. Non-specific binding ofthe antibody to other proteins may occur and may be detectable as a weak signal on the Western blot. The non-specific nature of this binding will be recognized by one skilled in the art by the weak signal obtained on the Western blot relative to the strong primary signal arising from the specific antibody-MATER protein binding. In certain embodiments, antibodies are generated which are specific to a domain or portion ofthe MATER protein.

Substantially pure MATER protein suitable for use as an immunogen is isolated from the transfected or transformed cells as described above. The concentration of protein in the final preparation is adjusted, for example, by concentration on an Amicon (Millipore, Bedford,

Massachusetts) or similar filter device, to the level of a few micrograms per milliliter. Monoclonal or polyclonal antibody to the protein can then be prepared as follows:

A. Monoclonal Antibody Production by Hybridoma Fusion

Monoclonal antibody to epitopes ofthe MATER protein identified and isolated as described can be prepared from murine hybridomas according to the classical method of Kohler and Milstein

(Nature 256:495-497, 1975) or derivative methods thereof. Briefly, a mouse is repetitively inoculated with a few micrograms ofthe selected protein over a period of a few weeks. The mouse is then sacrificed, and the antibody-producing cells ofthe spleen isolated. The spleen cells are fused with mouse myeloma cells using polyethylene glycol, and the excess un-fused cells destroyed by growth of the system on selective media comprising aminopterin (HAT media). Successfully fused cells are diluted and aliquots ofthe dilution placed in wells of a microtiter plate, where growth ofthe culture is continued. Antibody-producing clones are identified by detection of antibody in the supernatant fluid ofthe wells by immunoassay procedures, such as ELISA, as originally described by Engvall (Enzymol. 70(A):419-439, 1980), and derivative methods thereof. Selected positive clones can be expanded and their monoclonal antibody product harvested for use. Detailed procedures for monoclonal antibody production are described in Harlow and Lane (Antibodies, A Laboratory Manual, CSHL, New York, 1988).

B. Polyclonal Antibody Production by Immunization

Polyclonal antiserum containing antibodies to heterogeneous epitopes of a single protein can be prepared by immunizing suitable animals with the expressed protein (Example 8), which optionally can be modified to enhance immunogenicity. Effective polyclonal antibody production is affected by many factors related both to the antigen and the host species. For example, small molecules tend to be less immunogenic than others and may require the use of carriers and adjuvant, examples of which are known. Also, host animals vary in response to site of inoculations and dose, with either inadequate or excessive doses of antigen resulting in low titer antisera. A series of small doses (ng level) of antigen administered at multiple intradermal sites appear to be most reliable. An effective immunization protocol for rabbits can be found in Vaitukaitis et al. (J. Clin. Endocrinol. Metab. 33:988-991, 1971).

Booster injections can be given at regular intervals, and antiserum harvested when antibody titer thereof begins to fall, as determined semi-quantitatively (for example, by double immunodiffiision in agar against known concentrations ofthe antigen). See, for example, Ouchterlony et al (In Handbook of Experimental Immunology, Wier, D. (ed.) chapter 19. Blackwell, 1973). Plateau concentration of antibody is usually in the range of about 0.1 to 0.2 mg/ml of serum (about 12 μM). Affinity ofthe antisera for the antigen is determined by preparing competitive binding curves, as described, for example, by Fisher (Manual of Clinical Immunology, Ch. 42, 1980).

C. Antibodies Raised against Synthetic Peptides

A third approach to raising antibodies against the MATER protein is to use synthetic peptides synthesized on a commercially available peptide synthesizer based upon the predicted amino acid sequence ofthe MATER protein.

By way of example only, mouse MATER C-terminal peptide (residues 1093 through 1111 of SEQ ID NO: 6) was conjugated with KLH to immunize the female rabbits (two) every two-weeks. Starting from the third immunization, a small amount (-3 ml) of blood was collected from the immunized rabbits to examine the titer ofthe anti-peptide antibodies using the peptide as antigen and ELISA method. Immunizations continued until the antibodies reached maximal titers, which occurred in about ten immunizations, and then the rabbits were sacrificed to bleed in preparation for the sera. The resultant preparation was used both to characterize murine MATER (Example 1) and human MATER (Example 4). D. Antibodies Raised by Injection of MATER Encoding Sequence

Antibodies may be raised against the MATER protein by subcutaneous injection of a recombinant DNA vector that expresses the MATER protein into laboratory animals, such as mice. Delivery ofthe recombinant vector into the animals may be achieved using a hand-held form ofthe Biolistic system (Sanford et al, Particulate Sci. Techno! 5:27-37, 1987), as described by Tang et al (Nature 356: 152-154, 1992). Expression vectors suitable for this purpose may include those that express the MATER encoding sequence under the transcriptional control of either the human β-actin promoter or the cytomegalovirus (CMV) promoter.

Antibody preparations, both monoclonal and polyclonal, are useful in quantitative immunoassays which determine concentrations of antigen-bearing substances in biological samples; they are also used semi-quantitatively or qualitatively to identify the presence of antigen in a biological sample.

EXAMPLE 19: DNA-Based Diagnosis The Mater sequence information presented herein can be used in the area of genetic testing for predisposition to reduced fertility or infertility, such as autoimmune infertility, owing to defects in Mater, such as deletion, duplication or mutation. The gene sequence ofthe Mater gene, including intron-exon boundaries (listed in Table 2), is also useful in such diagnostic methods. Individuals carrying mutations in the Mater gene (or a portion thereof), or having duplications or heterozygous or homozygous deletions ofthe Mater gene, may be detected at the DNA level with the use of a variety of techniques. For such a diagnostic procedure, a biological sample ofthe subject, which biological sample contains either DNA or RNA derived from the subject, is assayed for a mutated, duplicated or deleted Mater gene. Suitable biological samples include samples containing genomic DNA or RNA obtained from body cells, such as those present in peripheral blood, urine, saliva, tissue biopsy, surgical specimen, amniocentesis samples and autopsy material. The detection in the biological sample of either a mutant Mater gene, a mutant Mater RNA, or a duplicated or homozygously or heterozygously deleted Mater gene, may be performed by a number of methodologies, examples of which are discussed below.

One embodiment of such detection techniques for the identification of unknown mutations is the amplification (e.g., polymerase chain reaction amplification) of reverse transcribed RNA (RT- PCR) isolated from a subject, followed by direct DNA sequence determination ofthe products. The presence of one or more nucleotide differences between the obtained sequence and the prototypical Mater cDNA sequence, and especially, differences in the ORF portion ofthe nucleotide sequence, are taken as indicative of a potential Mater gene mutation.

Alternatively, DNA extracted from a biological sample may be used directly for amplification. Direct amplification from genomic DNA would be appropriate for analysis ofthe entire Mater gene including regulatory sequences located upstream and downstream from the open reading frame, or intron/exon borders (see Table 2). By way of example, the intro-exon boundaries themselves can serve as sequences for oligonucleotide primers (or portions thereof) for us in in vitro amplification of such samples; representative examples of such ohgonucleotides are shown in SEQ ID NOs: 25-38. Reviews of direct DNA diagnosis have been presented by Caskey (Science 236:1223-1228, 1989) and by Landegren et al. (Science 242:229-237 , 1989). Other mutation scanning techniques appropriate for detecting unknown within amplicons derived from DNA or cDNA could also be performed. These techniques include direct sequencing (without sequencing), single-strand conformational polymorphism analysis (SSCP) (for instance, see Hongyo et al, Nucleic Acids Res. 21:3637-3642, 1993), chemical cleavage (including HOT cleavage) (Bateman et al, Am. J. Med. Genet. 45:233-240, 1993; reviewed in Ellis et al, Hum. Mutat. 11:345- 353, 1998), denaturing gradient gel electrophoresis (DGGE), ligation amplification mismatch protection (LAMP), and enzymatic mutation scanning (Taylor and Deeble, Genet. Anal. 14:181-186, 1999), followed by direct sequencing of amplicons with putative sequence variations.

Further studies of MATER genes isolated from female subjects displaying infertility, particularly autoimmune infertility subjects, or their relatives, may reveal particular mutations, genomic amplifications, or deletions, which occur at a high frequency within this population of individuals. In such case, rather than sequencing the entire Mater gene, DNA diagnostic methods can be designed to specifically detect the most common, or most closely disease-linked, MATER defects. The detection of specific DNA mutations may be achieved by methods such as hybridization using allele specific ohgonucleotides (ASOs) (Wallace et al, CSHL Symp. Quant. Biol. 51:257-261, 1986), ohgonucleotides specific for intron-exon boundaries (e.g., any one of SEQ ID NOs: 25-38), direct DNA sequencing (Church and Gilbert, Proc. Natl. Acad. Sci. USA 81:1991-1995, 1988), the use of restriction enzymes (Flavell et al, Cell 15:25-41, 1978; Geever et al, 1981), discrimination on the basis of electrophoretic mobility in gels with denaturing reagent (Myers and Maniatis, Cold Spring Harbor Symp. Quant. Biol. 51:275-284, 1986), RNase protection (Myers et al, Science 230:1242-1246, 1985), chemical cleavage (Cotton et al, Proc. Natl. Acad. Sci. USA 85:4397-4401, 1985), and the ligase-mediated detection procedure (Landegren et al, Science 241:1077-1080, 1988). In particular embodiments, the amount of complex formed between a MATER sequence and a MATER-specific oligonucleotide in a test sample (e.g. , a clinical sample from a subject) is compared to the amount of complex formed in a control or reference sample. Depending on the oligonucleotide selected, an increase in the formation of complex will be indicative ofthe presence of a biological condition associated with an abnormal MATER nucleic acid. In other circumstances, a decrease in the formation of complex will be indicative ofthe presence of a biological condition associated with an abnormal MATER nucleic acid. By way of example, in some embodiments, a wildtype oligonucleotide is used, and loss of complex formation is indicative of a mutation in the sample at the target regions. In other embodiments, a mutant or variant oligonucleotide (e.g., one comprising a known SNP), and an increase in complex formation is indicative ofthe presence ofthe mutation. Other combinations follow logically, including the reverse of these situations. Ohgonucleotides specific to normal or mutant sequences are chemically synthesized using commercially available machines. These ohgonucleotides are then labeled radioactively with isotopes (such as ³²P) or non-radioactively, with tags such as biotin (Ward and Langer, Proc. Natl. Acad. Sci. USA 78:6633-6657, 1981), and hybridized to individual DNA samples immobilized on membranes or other solid supports by dot-blot or transfer from gels after electrophoresis. These specific sequences are visualized by methods such as autoradiography or fluorometric (Landegren et al, Science 242:229-237, 1989) or colorimetric reactions (Gebeyehu et al, Nucleic Acids Res. 15:4513-4534, 1987). Using an ASO specific for a normal allele, the absence of hybridization would indicate a mutation in the particular region ofthe gene, or deleted Mater gene. In contrast, if an ASO specific for a mutant allele hybridizes to a clinical sample, this would indicate the presence of a mutation in the region defined by the ASO.

Sequence differences between normal and mutant forms ofthe MATER gene may also be revealed by the direct DNA sequencing method of Church and Gilbert (Proc. Natl. Acad. Sci. USA 81:1991-1995, 1988). Cloned DNA segments may be used as probes to detect specific DNA segments. The sensitivity of this method is greatly enhanced when combined with nucleic acid amplification, e.g., PCR (Wrichnik et al., Nucleic Acids Res. 15:529-542, 1987; Wong et al, Nature 330:384-386, 1987; Stoflet et al, Science 239:491-494, 1988). In this approach, a sequencing primer that lies within the amplified sequence is used with double-stranded PCR product or single-stranded template generated by a modified PCR. The sequence determination is performed by conventional procedures with radiolabeled nucleotides or by automatic sequencing procedures with fluorescent tags.

Sequence alterations may occasionally generate fortuitous restriction enzyme recognition sites or may eliminate existing restriction sites. Changes in restriction sites are revealed by the use of appropriate enzyme digestion followed by conventional gel-blot hybridization (Southern, J. Mol. Biol. 98:503-517, 1975). DNA fragments carrying the restriction site (either normal or mutant) are detected by their reduction in size or increase in corresponding restriction fragment numbers. Genomic DNA samples may also be amplified by PCR prior to treatment with the appropriate restriction enzyme; fragments of different sizes are then visualized under UV light in the presence of ethidium bromide after gel electrophoresis.

Genetic testing based on DNA sequence differences may be achieved by detection of alteration in electrophoretic mobility of DNA fragments in gels, with or without denaturing reagent. Small sequence deletions and insertions can be visualized by high-resolution gel electrophoresis. For example, a PCR product with small deletions is clearly distinguishable from a normal sequence on an 8% non-denaturing polyacrylamide gel (WO 91/10734; Nagamine et al, Am. J. Hum. Genet. 45:337- 339, 1989). DNA fragments of different sequence compositions may be distinguished on denaturing formamide gradient gels in which the mobilities of different DNA fragments are retarded in the gel at different positions according to their specific "partial-melting" temperatures (Myers et al, Science 230: 1242-1246, 1985). Alternatively, a method of detecting a mutation comprising a single base substitution or other small change could be based on differential primer length in a PCR. For example, an invariant primer could be used in addition to a primer specific for a mutation. The PCR products ofthe normal and mutant genes can then be differentially detected in acrylamide gels. In addition to conventional gel-electrophoresis and blot-hybridization methods, DNA fragments may also be visualized by methods where the individual DNA samples are not immobilized on membranes. The probe and target sequences may be both in solution, or the probe sequence may be immobilized (Saiki et al, Proc. Nat. Acad. Sci. USA 86:6230-6234, 1989). A variety of detection methods, such as autoradiography involving radioisotopes, direct detection of radioactive decay (in the presence or absence of scintillant), spectrophotometry involving calorigenic reactions and fluoromefry involved fluorogenic reactions, may be used to identify specific individual genotypes. See also the following patent documents, for instance: WO 89/10977; WO 97/27317; WO 97/29212; WO 98/28438; WO 99/05320; WO 99/47706; WO 00/47767; WO 00/56926; WO 00/79006; WO 01/83813; US 2002/0012930; US 5,700,637; US 6,228,575; US 6,300,063; and US 6,307,039.

If multiple mutations are encountered frequently in the MATER gene, a system capable of detecting such multiple mutations likely will be desirable. For example, a nucleic acid amplification reaction with multiple, specific oligonucleotide primers and hybridization probes may be used to identify all possible mutations at the same time (Chamberlain et al, Nucl Acids Res. 16: 1141-1155, 1988). The procedure may involve immobilized sequence-specific oligonucleotide probes (Saiki et al, Proc. Nat. Acad Sci. USA 86:6230-6234, 1989).

EXAMPLE 20:

Quantitation of MATER Protein

An alternative method of diagnosing Mater gene deletion, amplification, or mutation is to quantitate the level of MATER protein in the cells of a subject. This diagnostic tool would be useful for detecting reduced levels ofthe MATER protein that result from, for example, mutations in the promoter regions ofthe Mater gene or mutations within the coding region ofthe gene that produce truncated, non-functional or unstable polypeptides, as well as from deletions ofthe entire Mater gene. Alternatively, duplications ofthe Mater gene may be detected as an increase in the expression level of this protein. The determination of reduced or increased MATER protein levels would be an alternative or supplemental approach to the direct determination of Mater gene deletion, duplication or mutation status by the methods outlined above.

The availability of antibodies specific to the MATER protein will facilitate the quantitation of cellular MATER protein by one of a number of immunoassay methods, which are well known in the art and are presented in Harlow and Lane (Antibodies, A Laboratory Manual, CSHL, New York, 1988).

For the purposes of quantitating the MATER protein, a biological sample ofthe subject, which sample includes cellular proteins, is required. Such a biological sample may be obtained from body cells, such as those present in peripheral blood, urine, saliva, tissue biopsy, amniocentesis samples, surgical specimens and autopsy material. In particular, female reproductive cells (e.g., ova) or embryos are appropriate samples. Quantitation of MATER protein is achieved by immunoassay and compared to levels ofthe protein found in healthy cells (e.g., cells from a female known not to suffer from decreased fertility). A significant (e.g., 10% or greater, for instance, 20%, 25%, 30%, 50%) or more) reduction in the amount of MATER protein in the cells of a subject compared to the amount of MATER protein found in normal human cells would be taken as an indication that the subject may have deletions or mutations in the Mater gene locus, whereas a significant (e.g., 10% or greater, for instance, 20%, 25%), 30%>, 50% or more) increase would indicate that a duplication or enhancing mutation had occurred.

EXAMPLE 21:

Detection of Serum Antibody against MATER Protein

The MATER family of proteins was first identified as autoantibodies involved in the pathogenesis of certain cases of autoimmune infertility. With the provision herein of human MATER protein sequences and encoding nucleic acids, methods for the detection and diagnosis of such fertility failure are now enabled.

Autoantibodies that recognize an epitope ofthe human MATER protein can be detected in samples from a subject, for instance serum or other fluid, using known immunological techniques. The presence of such autoantibodies (e.g., circulating autoantibodies specific for a MATER epitope) indicates that the subject suffers from MATER-mediated infertility or reduced fertility, or has an increased susceptibility to suffer from one of these conditions.

Many techniques are commonly known in the art for the detection and quantification of antigen. Most commonly, the purified antigen will be bound to a substrate, the antibody ofthe sample will bind via its Fab portion to this antigen, the substrate will then be washed and a second, labeled antibody will then be added which will bind to the Fc portion ofthe antibody that is the subject ofthe assay. The second, labeled antibody will be species specific, i.e., ifthe serum is from a human, the second, labeled antibody will be anti-human-IgG antibody. The specimen will then be washed and the amount ofthe second, labeled antibody that has been bound will be detected and quantified by standard methods.

Examples of methods for the detection of antibodies in biological samples, including methods employing dip strips or other immobilized assay devices, are disclosed for instance in the following patents: U.S. Patents No. 5,965,356 (Herpes simplex virus type specific seroassay); 6, 114, 179 (Method and test kit for detection of antigens and or antibodies); 6,077,681 (Diagnosis of motor neuropathy by detection of antibodies); 6,057,097 (Marker for pathologies comprising an autoimmune reaction and/or for inflammatory diseases); and 5,552,285 (Immunoassay methods, compositions and kits for antibodies to oxidized DNA bases).

EXAMPLE 22: Suppression of MATER Expression

A reduction of MATER protein expression in a transgenic cell may be obtained by introducing into cells an antisense construct based on the Mater encoding sequence, including the human Mater cDNA or fragments thereof (for instance, the cDNA fragments shown in SEQ ID NO: 1 and 3) or gene sequence or flanking regions thereof. For antisense suppression, a nucleotide sequence from an MATER encoding sequence, e.g. all or a portion ofthe Mater cDNA or gene, is arranged in reverse orientation relative to the promoter sequence in the transformation vector. Other aspects ofthe vector may be chosen as discussed above (Example 8).

The introduced sequence need not be the full length human Mater cDNA (SEQ ID NO: 23) or gene, and need not be exactly homologous to the equivalent sequence found in the cell type to be transformed. Thus, portions or fragments ofthe murine cDNA (SEQ ID NO: 5) could also be used to knock out expression ofthe human Mater gene. Generally, however, where the introduced sequence is of shorter length, a higher degree of homology to the native Mater sequence will be needed for effective antisense suppression. The introduced antisense sequence in the vector may be at least 30 nucleotides in length, and improved antisense suppression typically will be observed as the length of the antisense sequence increases. The length of the antisense sequence in the vector advantageously may be greater than 100 nucleotides, and can be up to about the full length ofthe human Mater cDNA or gene. For suppression ofthe Mater gene itself, transcription of an antisense construct results in the production of RNA molecules that are the reverse complement of mRNA molecules transcribed from the endogenous Mater gene in the cell. Although the exact mechanism by which antisense RNA molecules interfere with gene expression has not been elucidated, it is believed that antisense RNA molecules bind to the endogenous mRNA molecules and thereby inhibit translation ofthe endogenous mRNA. Small inhibitory RNAs (siRNAs) can also be used. Suppression of endogenous MATER expression can also be achieved using ribozymes. Ribozymes are synthetic RNA molecules that possess highly specific endoribonuclease activity. The production and use of ribozymes are disclosed in U.S. Patent No. 4,987,071 to Cech and U.S. Patent No. 5,543,508 to Haselhoff. The inclusion of ribozyme sequences within antisense RNAs may be used to confer RNA cleaving activity on the antisense RNA, such that endogenous mRNA molecules that bind to the antisense RNA are cleaved, which in turn leads to an enhanced antisense inhibition of endogenous gene expression.

Finally, dominant negative mutant forms of MATER may be used to block endogenous MATER activity.

EXAMPLE 23: MATER Knockout and Overexpression Transgenic Animals

Mutant organisms that under-express or over-express MATER protein are useful for research. Such mutants allow insight into the physiological and/or pathological role of MATER in a healthy and/or pathological organism. These mutants are "genetically engineered," meaning that information in the form of nucleotides has been transferred into the mutant's genome at a location, or in a combination, in which it would not normally exist. Nucleotides transferred in this way are said to be "non-native." For example, a non-Mater promoter inserted upstream of a native Mater gene would be non-native. An extra copy of a Mater gene or other encoding sequence on a plasmid, transformed into a cell, would be non-native, whether that extra copy was Mater derived from the same or a different species.

Mutants may be, for example, produced from mammals, such as mice, that either over- express or under-express MATER protein, or that do not express MATER at all. Over-expression mutants are made by increasing the number of MATER-encoding sequences (such as genes) in the organism, or by introducing an MATER-encoding sequence into the organism under the control of a constitutive or inducible or viral promoter such as the mouse mammary tumor virus (MMTV) promoter or the whey acidic protein (WAP) promoter or the metallothionein promoter. Mutants that under-express MATER may be made by using an inducible or repressible promoter, or by deleting the Mater gene, or by destroying or limiting the function ofthe Mater gene, for instance by disrupting the gene by transposon insertion.

Antisense genes may be engineered into the organism, under a constitutive or inducible promoter, to decrease or prevent MATER expression, as discussed above in Example 13.

A gene is "functionally deleted" when genetic engineering has been used to negate or reduce gene expression to negligible levels. When a mutant is referred to in this application as having the Mater gene altered or functionally deleted, this refers to the Mater gene and to any ortholog of this gene. When a mutant is referred to as having "more than the normal copy number" of a gene, this means that it has more than the usual number of genes found in the wild-type organism, e.g., in the diploid mouse or human. A mutant mouse over-expressing MATER may be made by constructing a plasmid having the Mater gene driven by a promoter, such as the mouse mammary tumor virus (MMTV) promoter or the whey acidic protein (WAP) promoter. This plasmid may be introduced into mouse oocytes by microinjection. The oocytes are implanted into pseudopregnant females, and the litters are assayed for insertion ofthe fransgene. Multiple strains containing the fransgene are then available for study. WAP is quite specific for mammary gland expression during lactation, and MMTV is expressed in a variety of tissues including mammary gland, salivary gland and lymphoid tissues. Many other promoters might be used to achieve various patterns of expression, e.g., the metallothionein promoter. An inducible system may be created in which the subject expression construct is driven by a promoter regulated by an agent that can be fed to the mouse, such as tetracycline. Such techniques are well known in the art.

A mutant knockout animal (e.g, mouse) from which the Mater gene is deleted or otherwise disabled can be made by removing coding regions ofthe Mater gene from embryonic stem cells. The methods of creating deletion mutations by using a targeting vector have been described (see, for instance, Thomas and Capecch, Cell 51:503-512, 1987). One specific example ofthe production of a Mater null mouse is described above, in Example 1.

EXAMPLE 24: Gene Therapy

Gene therapy approaches for combating MATER-mediated fertility defects in subjects, or for causing MATER-mediated infertility in subjects, are now made possible.

Retroviruses have been considered the preferred vector for experiments in gene therapy, with a high efficiency of infection and stable integration and expression (Orkin et al, Prog. Med. Genet. 7:130-142, 1988). The full-length MATER gene or cDNA can be cloned into a retroviral vector and driven from either its endogenous promoter or, for instance, from the retroviral LTR (long terminal repeat). Other viral transfection systems may also be utilized for this type of approach, including adenovirus, adeno-associated virus (AAV) (McLaughlin et al, J. Virol. 62:1963-1973, 1988), Vaccinia virus (Moss etal., Annu. Rev. Immunol. 5:305-324, 1987), Bovine Papilloma virus (Rasmussen et al, Methods Enzymol 139:642-654, 1987) or members ofthe herpesvirus group such as Epstein-Barr virus (Margolskee et al, Mol. Cell. Biol. 8:2837-2847, 1988).

Recent developments in gene therapy techniques include the use of RNA-DNA hybrid ohgonucleotides, as described by Cole-Strauss, et al. (Science 273:1386-1389, 1996). This technique may allow for site-specific integration of cloned sequences, thereby permitting accurately targeted gene replacement.

In addition to delivery of MATER to cells using viral vectors, it is possible to use non- infectious methods of delivery. For instance, lipidic and liposome-mediated gene delivery has recently been used successfully for transfection with various genes (for reviews, see Templeton and Lasic, Mol Biotechnol. 11:175-180, 1999; Lee and Huang, Crit. Rev. Ther. Drug Carrier Syst. 14:173-206; and Cooper, Semin. Oncol. 23:172-187, 1996). For instance, cationic liposomes have been analyzed for their ability to transfect monocytic leukemia cells, and shown to be a viable alternative to using viral vectors (de Lima etal, Mol. Membr. Biol. 16:103-109, 1999). Such cationic liposomes can also be targeted to specific cells through the inclusion of, for instance, monoclonal antibodies or other appropriate targeting ligands (Kao et al, Cancer Gene Ther. 3:250- 256, 1996).

EXAMPLE 25: Identification of Therapeutic Compounds

The human MATER molecules disclosed herein can be used to identify (screen for) compounds that are useful in influencing MATER-mediated fertility in a mammal, either by blocking (inhibiting) the activity of MATER (and thereby reducing fertility) or enhancing MATER activity (and thereby increasing fertility). Such screening methods can include determining if a test compound binds directly to or otherwise interacts with a MATER protein, or a variant or fragment of a MATER protein. Proteins that do bind to such a molecule are select for further characterization.

In specific embodiments, the compound being tested for activity is applied to a cell for instance a test cell (e.g., a developing oocyte or embryo of a mammal). The activity ofthe MATER protein in that test cell is then measured, for instance by determining whether the oocyte or embryo progresses beyond the two-cell stage. If application ofthe test compound alters proportion of embryos that progress beyond two cells, then that compound is selected as a likely candidate for further characterization. In particular examples, a test agent that opposes or inhibits a MATER activity is selected for further study, for example by exposing the agent to a mammalian female reproductive system in vivo, to determine if in vivo fertility is inhibited. Such identified compounds may be useful as contraceptive agents.

Specific examples of compounds likely to be effective at inhibiting MATER activity, and therefore effective as contraceptives, include antibodies specifically directed to epitopes ofthe MATER protein. The general concept of immunocontraceptives has been described (see, e.g., U.S. Patents No. 5,637,300 and 6,027,727, describing contraceptive antibodies directed to proteins ofthe zona pellucida, and incorporated herein by reference).

Alternatively, compounds that increase the proportion of embryos that progress beyond two cells (for instance, in an animal system known to be defective for fertility) are selected for further study as possible fertility enhancing agents. Similar screens can be used to identify compounds that mimic the activity of MATER protein, for instance in a MA TER null animal.

In addition, it is suggested that MATER protein perform its function within the cytoplasm through interacting with other unknown protein. Physical blockage of such interaction is expected to arrest the MATER function. Candidates for such interactions are being identified based on interactions in a yeast two-hybrid system (Fields and Songs, Nature 340:245, 1989). Once molecular domains for the protein interaction are known, molecules can be designed directly based to specifically inhibit or otherwise interfere with MATER protein interactions with these other proteins. Compounds selected using these methods are comprehended by this disclosure.

EXAMPLE 26: Kits Kits are provided which contain the necessary reagents for detennining MATER gene copy number, such as probes or primers specific for the MATER gene, as well as written instructions. Kits are also provided to determine abnormal expression of MA TER mRNA (/. e. , containing probes) or MATER protein (i.e., containing a MATER-specific binding agent). Instructions provided in the diagnostic kits can include calibration curves or charts to compare with the determined (e.g., experimentally measured) values.

A. Kits for Detection of MATER Genomic Sequences

The nucleotide sequences disclosed herein, and fragments thereof, can be supplied in the form of a kit for use in detection of MATER genomic sequences and/or diagnosis of infertility or reduced fertility. In such a kit, an appropriate amount of one or more ofthe MA TER-soecific oligonucleotide primers is provided in one or more containers. The oligonucleotide primer(s) (for instance, particular ohgonucleotides disclosed herein) may be provided suspended in an aqueous solution or as a freeze-dried or lyophilized powder, for instance. The container(s) in which the oligonucleotide(s) are supplied can be any conventional container that is capable of holding the supplied form, for instance, microfuge tubes, ampoules, or bottles. In some applications, pairs of primers may be provided in pre-measured single use amounts in individual, typically disposable, tubes or equivalent containers. With such an arrangement, the sample to be tested for the presence of MATER genomic amplification, or deletion, can be added to the individual tubes and in vitro amplification carried out directly.

The amount of each oligonucleotide primer supplied in the kit can be any appropriate amount, depending for instance on the market to which the product is directed. For instance, ifthe kit is adapted for research or clinical use, the amount of each oligonucleotide primer provided would likely be an amount sufficient to prime several in vitro amplification reactions. Those of ordinary skill in the art know the amount of oligonucleotide primer that is appropriate for use in a single amplification reaction. General guidelines may for instance be found in Innis et al. (PCR Protocols, A Guide to Methods and Applications, Academic Press, Inc., San Diego, CA, 1990), Sambrook et al (In Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York, 1989), and Ausubel et al. (In Current Protocols in Molecular Biology, John Wiley & Sons, New York, 1998). A kit may include more than two primers, in order to facilitate the PCR in vitro amplification of MATER sequences, for instance the MATER gene, specific exon(s) or other portions ofthe gene (e.g., a portion bridging an intron-exon boundary), or the 5' or 3' flanking region thereof.

In some embodiments, kits may also include the reagents necessary to carry out in vitro amplification reactions, such as PCR amplification, including, for instance, DNA sample preparation reagents, appropriate buffers (e.g., polymerase buffer), salts (e.g., magnesium chloride), and deoxyribonucleotides (dNTPs). Written instructions may also be included.

Kits may in addition include either labeled or unlabeled oligonucleotide probes for use in detection ofthe in vitro amplified MATER sequences. The appropriate sequences for such a probe will be any sequence that falls between the annealing sites ofthe two provided oligonucleotide primers, such that the sequence the probe is complementary to is amplified during the in vitro amplification reaction.

It may also be advantageous to provided in the kit one or more control sequences for use in the amplification reactions. The design of appropriate positive control sequences is well known to one of ordinary skill in the appropriate art.

B. Kits for Detection of Mαter mRNA Expression

Kits similar to those disclosed above for the detection of MATER genomic sequences can be used to detect MATER mRNA expression levels. Such kits may include an appropriate amount of one or more ofthe oligonucleotide primers for use in reverse transcription amplification reactions, similarly to those provided above, with art-obvious modifications for use with RNA.

In some embodiments, kits for detection of MATER mRNA expression levels may also include the reagents necessary to carry out RT-PCR in vitro amplification reactions, including, for instance, RNA sample preparation reagents (including e.g., an RNAse inhibitor), appropriate buffers (e.g., polymerase buffer), salts (e.g., magnesium chloride), and deoxyribonucleotides (dNTPs). Written instructions may also be included.

Kits in addition may include either labeled or unlabeled oligonucleotide probes for use in detection ofthe in vitro amplified target sequences. The appropriate sequences for such a probe will be any sequence that falls between the annealing sites ofthe two provided oligonucleotide primers, such that the sequence the probe is complementary to is amplified during the PCR reaction, or another in vitro amplification reaction.

It also may be advantageous to provided in the kit one or more control sequences for use in the RT-PCR reactions. The design of appropriate positive control sequences is well known to one of ordinary skill in the appropriate art.

Alternatively, kits may be provided with the necessary reagents to carry out quantitative or semi-quantitative Northern analysis of MATER mRNA. Such kits include, for instance, at least one M47£/?-specific oligonucleotide for use as a probe. This oligonucleotide may be labeled in any conventional way, including with a selected radioactive isotope, enzyme substrate, co-factor, ligand, chemiluminescent or fluorescent agent, hapten, or enzyme. C. Kits For Detection of MATER Protein or Peptide Expression

Kits for the detection of MATER protein expression, for instance MATER at least one target (e.g., MATER) protein specific binding agent (e.g, a polyclonal or monoclonal antibody or antibody fragment) and may include at least one control. The MATER protein specific binding agent and control may be contained in separate containers. The kits may also include means for detecting

MATER:agent complexes, for instance the agent may be detectably labeled. Ifthe detectable agent is not labeled, it may be detected by second antibodies or protein A for example which may also be provided in some kits in one or more separate containers. Such techniques are well known. Additional components in some kits include instructions for carrying out the assay. Instructions will allow the tester to determine whether MATER expression levels are altered, for instance in comparison to a control sample. Reaction vessels and auxiliary reagents such as chromogens, buffers, enzymes, etc. may also be included in the kits.

By way of example only, an effective and convenient immunoassay kit such as an enzyme- linked immunosorbent assay can be constructed to test anti-MATER antibody in human serum, as reported for detection of non-specific anti-ovarian antibodies (Wheatcroft et al, Clin. Exp. Immunol. 96:122-128, 1994; Wheatcroft et al, Hum. Reprod. 12:2617-2622, 1997). Expression vectors can be constructed using the human MATER cDNA to produce the recombinant human MATER protein in either bacteria or baculovirus (as described in Example 8). By affinity purification, unlimited amounts of pure recombinant MATER protein can be produced. An assay kit could provide the recombinant protein as an antigen and enzyme-conjugated goat anti-human IgG as a second antibody as well as the enzymatic substrates. Such kits can be used to test ifthe patient sera contain antibodies against human MATER.

This disclosure provides MATER nucleic acids and proteins, including the human Mater molecules described above. The disclosure further provides methods employing these molecules, including methods to predict and/or diagnose infertility, reduced fertility, or reproductive failure in females, as well as treatments for such infertility and reduced fertility, and contraceptives. It will be apparent that the precise details ofthe molecules and methods described may be varied or under- or overexpression, are also contemplated. Such kits will include modified without departing from the spirit ofthe described invention. The inventors claim all such modifications and variations that fall within the scope and spirit ofthe claims below.

Claims

1. An isolated variant MATER protein comprising an amino acid sequence

(a) having at least 90% sequence identity to SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 24, and comprising at least one variant amino acid in a recognized MATER protein domain; or

(b) having at least 90% sequence identity to SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 24, and comprising at least one variant amino acid in a region outside of a recognized MATER protein domain; or

(c) having at least 90% sequence identity to SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 24, and comprising at least one variant amino acid in a recognized MATER protein domain and at least one variant amino acid outside of a recognized MATER protein domain.

,

2. The isolated variant MATER protein of claim 1, wherein the sequence ofthe protein comprises at least one variant amino acid in a Leucine-rich repeat.

3. The isolated variant MATER protein of claim 1, wherein the sequence ofthe protein comprises at least one variant amino acid in the PAAD-DAPIN domain.

4. The isolated variant MATER protein of claim 1 , wherein the sequence ofthe protein comprises at least one variant amino acid in the aldo_ket_red domain.

5. The isolated variant MATER protein of claim 1, wherein the sequence ofthe protein comprises at least one variant amino acid in the ATP-/GTP-binding domain.

6. The isolated variant MATER protein of claim 1, wherein the sequence ofthe protein comprises at least one variant amino acid outside of a recognized MATER protein domain.

7. An isolated nucleic acid molecule encoding a protein according to claim 1.

8. A recombinant nucleic acid molecule comprising a promoter sequence operably linked to nucleic acid molecule according to claim 7.

9. A cell transformed with a recombinant nucleic acid molecule according to claim 8.

10. A method of detecting in a subject a biological condition associated with an abnormal MATER nucleic acid, comprising reacting at least one MATER nucleic acid in a clinical sample from the subject with a reagent comprising a 4 ER-specific nucleic acid binding agent to form a MATER:agent complex, and detecting the complex.

11. The method of claim 10, wherein the MA TER-sυecific nucleic acid binding agent is a MATER oligonucleotide.

12. The method of claim 11, wherein the MATER oligonucleotide comprises at least 10 contiguous nucleotides of a human MATER intron sequence.

13 The method of claim 11, wherein the MATER oligonucleotide comprises a sequence as shown in any one of SEQ ID NOs: 25 through 38.

14. The method of claim 10, further comprising quantifying the MATER-.agent complex and comparing the quantity ofthe MATER agent complex with the quantity of an equivalent MATER: agent complex formed in a reference sample.

15. The method of claim 14, wherein the reference sample is a sample from a reference subject known to have the biological condition.

16. The method of claim 14, wherein the reference sample is a sample from a reference subject known not to have the biological condition.

17. The method of claim 10, wherein the clinical sample comprises a reproductive cell.

18. The method of claim 10, wherein the method is used for detecting a predisposition to infertility or reduced fertility or for presymptomatic screening of an individual for infertility or reduced fertility.

19. The method of claim 10, which is a method of evaluating a cause of female infertility.

20. The method of claim 10, further comprising in vitro amplifying a Mater nucleic acid prior to detecting the abnormal Mater nucleic acid.

21. The method of claim 10, wherein the Mater nucleic acid is in vitro amplified using at least one oligonucleotide primer derived from a MATER protein encoding sequence.

22. The method of claim 10, wherein the oligonucleotide primer comprises a sequence comprising an intron-exon boundary of human MATER.

23. The method of claim 22, wherein the oligonucleotide primer comprises a sequence as shown in any on of SEQ ID NOs: 25 through 38.

24. An oligonucleotide primer used in the method of claim 22.

25. The method of claim 10, wherein the Λ£477i ?: agent complex is detected by nucleotide hybridization.

26. The method of claim 10, wherein the agent is a labeled nucleotide probe.

27. The method of claim 26, wherein the nucleotide probe comprises a sequence as shown in any one of SEQ ID NOs: 25 through 38.

28. A labeled nucleotide probe used in the method of claim 26.

29. A kit for detection of a genetic mutation in a sample of nucleic acid, comprising: (a) a first container containing an oligonucleotide capable of specifically hybridizing with a MATER nucleic acid, which oligonucleotide has a sequence comprising at least 10 contiguous nucleotides from an human MATER intron; and (b) a second container containing a fluorescent labeled nucleic acid probe that is fully complementary to the oligonucleotide.

30. The kit of claim 29, wherein the fluorescent labeled nucleic acid probe has a length of between 5 and 500 nucleotides.

31. The kit of claim 29, wherein the oligonucleotide comprises a sequence as shown in any one of SEQ ID NOs: 25 through 38.