WO2002072879A3 - Erzeugung und verwendung von zufallsanordnungen klonaler nukleinsäureinseln auf einer oberfläche - Google Patents

Erzeugung und verwendung von zufallsanordnungen klonaler nukleinsäureinseln auf einer oberfläche Download PDF

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Publication number
WO2002072879A3
WO2002072879A3 PCT/EP2002/001406 EP0201406W WO02072879A3 WO 2002072879 A3 WO2002072879 A3 WO 2002072879A3 EP 0201406 W EP0201406 W EP 0201406W WO 02072879 A3 WO02072879 A3 WO 02072879A3
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WO
WIPO (PCT)
Prior art keywords
primer
clonal
islands
amplified
amplification
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PCT/EP2002/001406
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English (en)
French (fr)
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WO2002072879A2 (de
Inventor
Achim Fischer
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Axaron Bioscience Ag
Achim Fischer
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Publication date
Application filed by Axaron Bioscience Ag, Achim Fischer filed Critical Axaron Bioscience Ag
Publication of WO2002072879A2 publication Critical patent/WO2002072879A2/de
Publication of WO2002072879A3 publication Critical patent/WO2002072879A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Erzeugung einer Zufallsanordnung klonaler Inseln auf einer Oberfläche, umfassend die Schritte a1) Bereitstellung einer Oberfläche aufweisend mindestens einen Primer-Typ, dessen Primer-Moleküle mindestens zum Teil irreversibel an der Oberfläche immobilisiert sind; b1) Hybridisierung von zu amplifizierenden Nukleinsäuren mit den Primern aus Schritt a1); c1) Amplifikation der zu amplifizierenden Nukleinsäuren aus Schritt b1), wobei mindestens ein Gegenprimer-Typ zur Amplifikation eingesetzt wird, dessen Primer-Moleküle mindestens zum Teil nicht immobilisiert sind und dessen Primer-Moleküle mit den Primern aus Schritt a1) in bezug auf die zu amplifizierenden Nukleinsäuren mindestens ein PCR-Primerpaar bilden, und wobei der Reaktionsraum der Amplifikation durch einen Raum gebildet wird, der durch die Oberfläche und durch eine an die Oberfläche angrenzende mikrokompartimentierende Matrix begrenzt wird.
PCT/EP2002/001406 2001-02-09 2002-02-11 Erzeugung und verwendung von zufallsanordnungen klonaler nukleinsäureinseln auf einer oberfläche WO2002072879A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10106320A DE10106320A1 (de) 2001-02-09 2001-02-09 Erzeugung und Verwendung von Zufallsanordnungen klonaler Nukleinsäureinseln auf einer Oberfläche
DE10106320.2 2001-02-09

Publications (2)

Publication Number Publication Date
WO2002072879A2 WO2002072879A2 (de) 2002-09-19
WO2002072879A3 true WO2002072879A3 (de) 2003-10-02

Family

ID=7673670

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/001406 WO2002072879A2 (de) 2001-02-09 2002-02-11 Erzeugung und verwendung von zufallsanordnungen klonaler nukleinsäureinseln auf einer oberfläche

Country Status (2)

Country Link
DE (1) DE10106320A1 (de)
WO (1) WO2002072879A2 (de)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993008305A1 (en) * 1991-10-17 1993-04-29 Dynal As Method of sequencing double stranded dna
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
WO1998044151A1 (en) * 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
WO1999019341A1 (en) * 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US5958698A (en) * 1992-10-26 1999-09-28 Institut Belka Method for amplification and expression of nucleic acids in solid media and its application for nucleic acid cloning and diagnostics
US6017738A (en) * 1991-11-01 2000-01-25 Adelaide Children's Hospital Solid phase amplification process
WO2000032809A2 (de) * 1998-11-27 2000-06-08 Noxxon Pharma Ag Kopieren und klonieren an oberflächen
WO2001048184A2 (de) * 1999-12-23 2001-07-05 Axaron Bioscience Ag Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090592A (en) * 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
WO1993008305A1 (en) * 1991-10-17 1993-04-29 Dynal As Method of sequencing double stranded dna
US6017738A (en) * 1991-11-01 2000-01-25 Adelaide Children's Hospital Solid phase amplification process
US5958698A (en) * 1992-10-26 1999-09-28 Institut Belka Method for amplification and expression of nucleic acids in solid media and its application for nucleic acid cloning and diagnostics
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
WO1998044151A1 (en) * 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
WO1999019341A1 (en) * 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
WO2000032809A2 (de) * 1998-11-27 2000-06-08 Noxxon Pharma Ag Kopieren und klonieren an oberflächen
WO2001048184A2 (de) * 1999-12-23 2001-07-05 Axaron Bioscience Ag Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KAHL, JD AND GREENBERG MM, JOURNAL OF ORGANIC CHEMISTRY, vol. 64, no. 2, 1999, pages 507 - 510, XP002216020 *
YERSHOV G ET AL: "DNA ANALYSIS AND DIAGNOSTICS ON OLIGONUCLEOTIDE MICROCHIPS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 93, 1 May 1996 (1996-05-01), pages 4913 - 4918, XP000196978, ISSN: 0027-8424 *
YOO DJ, GREENBERG MM, JOURNAL OF ORGANIC CHEMISTRY, vol. 60, no. 11, 1995, pages 3358 - 3364, XP002216021 *

Also Published As

Publication number Publication date
WO2002072879A2 (de) 2002-09-19
DE10106320A1 (de) 2002-08-22

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