WO2002068454A3 - Proteins in a porous support - Google Patents

Proteins in a porous support Download PDF

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Publication number
WO2002068454A3
WO2002068454A3 PCT/US2002/005755 US0205755W WO02068454A3 WO 2002068454 A3 WO2002068454 A3 WO 2002068454A3 US 0205755 W US0205755 W US 0205755W WO 02068454 A3 WO02068454 A3 WO 02068454A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
matrix material
activity
methods
porous matrix
Prior art date
Application number
PCT/US2002/005755
Other languages
French (fr)
Other versions
WO2002068454A2 (en
Inventor
Eric J Ackerman
Jun Liu
Lei Chenghong
Original Assignee
Battelle Memorial Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/791,138 external-priority patent/US7846684B2/en
Application filed by Battelle Memorial Institute filed Critical Battelle Memorial Institute
Priority to AU2002240515A priority Critical patent/AU2002240515A1/en
Priority to JP2002567964A priority patent/JP2005504505A/en
Priority to CA002438901A priority patent/CA2438901A1/en
Priority to EP02706428A priority patent/EP1402011A2/en
Publication of WO2002068454A2 publication Critical patent/WO2002068454A2/en
Publication of WO2002068454A3 publication Critical patent/WO2002068454A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A protein system is described in which a protein is bound within a matrix material that has pores that are sized to achieve excellent properties such as: activity, protein density, and stability. In a preferred embodiment, the pore sizes range from 50 to 400 A. One protein that has demonstrated surprisingly good results in this system is OPH. This protein is known to degrade organophosphorus compounds such as are found in chemical weapons and pesticides. Novel methods of forming the protein system and methods of making OPH are also described. Also described is a protein system including a porous matrix material and a protein disposed within the porous matrix material; wherein the protein system contains at least 0.01 mmol of protein per gram of matrix material and exhibits an activity at least 2 times greater that the activity of a protein system that has been formed under identical conditions on a normal silica matrix material. Methods of making protein systems with noncovalent bonding to entrap proteins in the porous matrix are also described.
PCT/US2002/005755 2001-02-21 2002-02-21 Proteins in a porous support WO2002068454A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002240515A AU2002240515A1 (en) 2001-02-21 2002-02-21 Proteins in a porous support
JP2002567964A JP2005504505A (en) 2001-02-21 2002-02-21 Protein in porous carrier
CA002438901A CA2438901A1 (en) 2001-02-21 2002-02-21 Proteins in a porous support
EP02706428A EP1402011A2 (en) 2001-02-21 2002-02-21 Proteins in a porous support

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/791,138 US7846684B2 (en) 2001-02-21 2001-02-21 Enzyme system comprising an enzyme bonded in a porous matrix
US09/791,138 2001-02-21
US8173702A 2002-02-20 2002-02-20
US10/081,737 2002-02-20

Publications (2)

Publication Number Publication Date
WO2002068454A2 WO2002068454A2 (en) 2002-09-06
WO2002068454A3 true WO2002068454A3 (en) 2003-12-24

Family

ID=26765910

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/005755 WO2002068454A2 (en) 2001-02-21 2002-02-21 Proteins in a porous support

Country Status (5)

Country Link
EP (1) EP1402011A2 (en)
JP (1) JP2005504505A (en)
AU (1) AU2002240515A1 (en)
CA (1) CA2438901A1 (en)
WO (1) WO2002068454A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1534421B1 (en) 2002-08-15 2006-09-27 Velocys, Inc. Tethered catalyst processes in microchannel reactors and systems containing a tethered catalyst or tethered chiral auxiliary
GB0315012D0 (en) * 2003-06-27 2003-07-30 Leuven K U Res & Dev Zeotiles
US8273371B2 (en) 2003-06-27 2012-09-25 Johan Adriaan Martens Crystalline mesoporous oxide based materials useful for the fixation and controlled release of drugs
EP1525890A1 (en) 2003-10-02 2005-04-27 Complex Biosystems GmbH Protein-Proteophore complexes
WO2005059125A1 (en) * 2003-12-16 2005-06-30 Commonwealth Scientific And Industrial Research Organisation Variants of phosphotriesterases with enhanced and/or altered substrate specificity
US7147912B2 (en) * 2004-08-18 2006-12-12 E. I. Du Pont De Nemours And Company Amphipathic proteinaceous coating on nanoporous polymer
WO2010036520A1 (en) 2008-09-26 2010-04-01 Wisconsin Alumni Research Foundation Mesoporous metal oxide materials for phosphoproteomics

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ACKERMAN E J ET AL: "Enzymatic Microreactors", 4TH INTERNATIONAL CONFERENCE ON MICROREACTION TECHNOLOGY, 8 March 2000 (2000-03-08), XP002253403, Retrieved from the Internet <URL:http://www.aiche.org/conferences/techprogram/paperdetail.asp?PaperID=551&DSN=spring2k> [retrieved on 20030903] *
RICHINS RICHARD D ET AL: "Expression, immobilization, and enzymatic characterization of cellulose-binding domain-organophosphorus hydrolase fusion enzymes.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 69, no. 6, 20 September 2000 (2000-09-20), pages 591 - 596, XP002253402, ISSN: 0006-3592 *
See also references of EP1402011A2 *
SINGH A K ET AL: "Development of sensors for direct detection of organophosphates. Part I: Immobilization, characterization and stabilization of acetylcholinesterase and organophosphate hydrolase on silica supports.", BIOSENSORS & BIOELECTRONICS, vol. 14, no. 8-9, December 1999 (1999-12-01), pages 703 - 713, XP002243724, ISSN: 0956-5663 *
TAKAHASHI H ET AL: "Catalytic activity in organic solvents and stability of immobilized enzymes depend on pore size and surface characteristics of mesoporous silica.", CHEMISTRY OF MATERIALS., vol. 12, 2 November 2000 (2000-11-02), AMERICAN CHEMICAL SOCIETY, WASHINGTON., US, pages 3301 - 3305, XP002243723, ISSN: 0897-4756 *
TAUPIN C ET AL: "SUREXPRESSION DANS E. COLI ET PURIFICATION D'UNE ENZYME BACTÉRIENNE DÉGRADANT LES ORGANOPHOSPHORÉS, LA PHOSPHOTRIESTÉRASE DE FLAVOBACTERIUM SP.", TRAVAUX SCIENTIFIQUES DES CHERCHEURS DU SERVICE DE SANTE DES ARMEES, no. 18, 1997, pages 177 - 178, XP008021557, ISSN: 0243-7473 *
YAN AI-XIN ET AL: "Recent progress on immobilization of enzymes on molecular sieves for reactions in organic solvents.", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 101, no. 2, May 2002 (2002-05-01), pages 113 - 129, XP008017666, ISSN: 0273-2289 *
YIU HUMPHREY H P ET AL: "Enzyme immobilisation using SBA-15 mesoporous molecular sieves with functionalised surfaces.", JOURNAL OF MOLECULAR CATALYSIS B ENZYMATIC, vol. 15, no. 1-3, 28 September 2001 (2001-09-28), pages 81 - 92, XP002243725, ISSN: 1381-1177 *

Also Published As

Publication number Publication date
WO2002068454A2 (en) 2002-09-06
CA2438901A1 (en) 2002-09-06
JP2005504505A (en) 2005-02-17
AU2002240515A1 (en) 2002-09-12
EP1402011A2 (en) 2004-03-31

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