WO2002066599A3 - Procede efficace permettant de mettre au point des plantes transgeniques par manipulation genetique - Google Patents

Procede efficace permettant de mettre au point des plantes transgeniques par manipulation genetique Download PDF

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Publication number
WO2002066599A3
WO2002066599A3 PCT/KR2002/000232 KR0200232W WO02066599A3 WO 2002066599 A3 WO2002066599 A3 WO 2002066599A3 KR 0200232 W KR0200232 W KR 0200232W WO 02066599 A3 WO02066599 A3 WO 02066599A3
Authority
WO
WIPO (PCT)
Prior art keywords
development
gene manipulation
transgenic plants
efficient method
transforming
Prior art date
Application number
PCT/KR2002/000232
Other languages
English (en)
Other versions
WO2002066599A2 (fr
Inventor
Yang-Do Choi
Hak-Soo Seo
Jong-Tae Song
Jong-Joo Cheong
Jong-Seob Lee
Yeon-Jong Koo
Original Assignee
Scigen Harvest Co Ltd
Yang-Do Choi
Hak-Soo Seo
Jong-Tae Song
Jong-Joo Cheong
Jong-Seob Lee
Yeon-Jong Koo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scigen Harvest Co Ltd, Yang-Do Choi, Hak-Soo Seo, Jong-Tae Song, Jong-Joo Cheong, Jong-Seob Lee, Yeon-Jong Koo filed Critical Scigen Harvest Co Ltd
Priority to AU2002234979A priority Critical patent/AU2002234979A1/en
Publication of WO2002066599A2 publication Critical patent/WO2002066599A2/fr
Publication of WO2002066599A3 publication Critical patent/WO2002066599A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Abstract

La présente invention concerne un procédé permettant de transformer des plantes par manipulation génétique, et plus particulièrement, un procédé permettant de transformer des plantes avec une agrobactérie contenant un gène bar présentant une résistance à un herbicide, par une méthode de lésion efficace sur un tissu cible approprié. Le procédé de transformation décrit dans cette invention permet d'obtenir une race de plante améliorée par rapport à une race de plante classique.
PCT/KR2002/000232 2001-02-16 2002-02-14 Procede efficace permettant de mettre au point des plantes transgeniques par manipulation genetique WO2002066599A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002234979A AU2002234979A1 (en) 2001-02-16 2002-02-14 Efficient method for the development of transgenic plants by gene manipulation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR2001/7818 2001-02-16
KR10-2001-0007818A KR100402513B1 (ko) 2001-02-16 2001-02-16 유전자 조작을 통한 식물체의 효과적인 형질전환 방법

Publications (2)

Publication Number Publication Date
WO2002066599A2 WO2002066599A2 (fr) 2002-08-29
WO2002066599A3 true WO2002066599A3 (fr) 2002-11-28

Family

ID=19705832

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2002/000232 WO2002066599A2 (fr) 2001-02-16 2002-02-14 Procede efficace permettant de mettre au point des plantes transgeniques par manipulation genetique

Country Status (3)

Country Link
KR (1) KR100402513B1 (fr)
AU (1) AU2002234979A1 (fr)
WO (1) WO2002066599A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8937216B2 (en) 2007-03-09 2015-01-20 Monsanto Technology Llc Method of meristem excision and transformation
CN106636196B (zh) * 2017-02-21 2019-11-19 辽宁省农业科学院大连生物技术研究所 一种优化的、高效率的农杆菌介导的花生转化方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
WO2010065534A2 (fr) * 2008-12-01 2010-06-10 Ceres, Inc. Procédés de transformation de miscanthus
EP2734035A4 (fr) * 2011-07-22 2015-03-25 Basf Plant Science Co Gmbh Procédé de transformation de plante
CN105132457B (zh) * 2015-10-19 2018-08-03 宁夏农林科学院 一种快速遗传转化苜蓿的方法
CN111218471A (zh) * 2020-02-23 2020-06-02 华中农业大学 发根农杆菌介导南瓜根系转化方法及基因编辑方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006844A1 (fr) * 1999-07-22 2001-02-01 Japan As Represented By Director General Of National Institute Of Agrobiological Resources Ministry Of Agriculture, Forestry And Fisheries Procede de transformation ultrarapide de monocotyledon

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5416011A (en) * 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
EP0652965A1 (fr) * 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. Procede ameliore de transformation induite par agrobacterium de cellules de soja cultivees
WO1995015678A1 (fr) * 1993-12-09 1995-06-15 The Texas A & M University System TRANSFORMATION D'ESPECES MUSA AU MOYEN $i(D'AGROBACTERIUM TUMEFACIENS)
US5693512A (en) * 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006844A1 (fr) * 1999-07-22 2001-02-01 Japan As Represented By Director General Of National Institute Of Agrobiological Resources Ministry Of Agriculture, Forestry And Fisheries Procede de transformation ultrarapide de monocotyledon

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GENETIKA, vol. 36, no. 7, July 2000 (2000-07-01), pages 932 - 941 *
PLANT PHYSIOL., vol. 123, no. 3, July 2000 (2000-07-01), pages 1069 - 1076 *
TRANSGENIC RES., vol. 9, no. 2, April 2000 (2000-04-01), pages 103 - 113 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8937216B2 (en) 2007-03-09 2015-01-20 Monsanto Technology Llc Method of meristem excision and transformation
US9006513B2 (en) 2007-03-09 2015-04-14 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
US11566252B2 (en) 2007-03-09 2023-01-31 Monsanto Technology, Llc Preparation and use of plant embryo explants for transformation
CN106636196B (zh) * 2017-02-21 2019-11-19 辽宁省农业科学院大连生物技术研究所 一种优化的、高效率的农杆菌介导的花生转化方法

Also Published As

Publication number Publication date
AU2002234979A1 (en) 2002-09-04
WO2002066599A2 (fr) 2002-08-29
KR20020067303A (ko) 2002-08-22
KR100402513B1 (ko) 2003-10-22

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