WO2002062131A3 - Methode de clonage d'animaux mammiferes transgeniques a l'aide de pseudo-noyaux - Google Patents

Methode de clonage d'animaux mammiferes transgeniques a l'aide de pseudo-noyaux Download PDF

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Publication number
WO2002062131A3
WO2002062131A3 PCT/US2002/003360 US0203360W WO02062131A3 WO 2002062131 A3 WO2002062131 A3 WO 2002062131A3 US 0203360 W US0203360 W US 0203360W WO 02062131 A3 WO02062131 A3 WO 02062131A3
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WO
WIPO (PCT)
Prior art keywords
pseudonuclei
mammalian animals
animals
transgenic mammalian
cybrids
Prior art date
Application number
PCT/US2002/003360
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English (en)
Other versions
WO2002062131A8 (fr
WO2002062131A2 (fr
Inventor
Gregory H Leno
Erik J Forsberg
Original Assignee
Infigen Inc
Gregory H Leno
Erik J Forsberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Infigen Inc, Gregory H Leno, Erik J Forsberg filed Critical Infigen Inc
Priority to AU2002250020A priority Critical patent/AU2002250020A1/en
Publication of WO2002062131A2 publication Critical patent/WO2002062131A2/fr
Publication of WO2002062131A3 publication Critical patent/WO2002062131A3/fr
Publication of WO2002062131A8 publication Critical patent/WO2002062131A8/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des méthodes permettant de préparer des embryons transgéniques, des foetus, et des animaux clonés à l'aide de méthodes de transfert nucléaire. On prépare des cybrides par translocation d'au moins un pseudo-noyau ou d'une pseudo-cellule, et d'une cellule donneuse nucléaire ou d'un noyau cellulaire dans une cellule réceptrice. Il est possible d'utiliser les cybrides résultants pour préparer lesdits embryons transgéniques, foetus, et animaux clonés de l'invention.
PCT/US2002/003360 2001-02-02 2002-02-01 Methode de clonage d'animaux mammiferes transgeniques a l'aide de pseudo-noyaux WO2002062131A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002250020A AU2002250020A1 (en) 2001-02-02 2002-02-01 Method of cloning transgenic mammalian animals using pseudonuclei

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26602301P 2001-02-02 2001-02-02
US60/266,023 2001-02-02

Publications (3)

Publication Number Publication Date
WO2002062131A2 WO2002062131A2 (fr) 2002-08-15
WO2002062131A3 true WO2002062131A3 (fr) 2002-10-10
WO2002062131A8 WO2002062131A8 (fr) 2003-05-08

Family

ID=23012845

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/003360 WO2002062131A2 (fr) 2001-02-02 2002-02-01 Methode de clonage d'animaux mammiferes transgeniques a l'aide de pseudo-noyaux

Country Status (2)

Country Link
AU (1) AU2002250020A1 (fr)
WO (1) WO2002062131A2 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998007841A1 (fr) * 1996-08-19 1998-02-26 University Of Massachusetts Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire croisee d'especes
WO1999045100A1 (fr) * 1998-03-02 1999-09-10 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Lignees cellulaires embryonnaires ou de type souche produites par transplantation nucleaire d'especes croisees
US6147276A (en) * 1995-08-31 2000-11-14 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer in the production of non-human mammals and non-human mammalian embryos
US6235970B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts, Amherst Campus CICM cells and non-human mammalian embryos prepared by nuclear transfer of a proliferating differentiated cell or its nucleus
US6258998B1 (en) * 1998-11-24 2001-07-10 Infigen, Inc. Method of cloning porcine animals
US20010037513A1 (en) * 2000-01-04 2001-11-01 Xiangzhong Yang Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
US6395958B1 (en) * 1997-03-06 2002-05-28 Infigen, Inc. Method of producing a polypeptide in an ungulate

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6147276A (en) * 1995-08-31 2000-11-14 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer in the production of non-human mammals and non-human mammalian embryos
WO1998007841A1 (fr) * 1996-08-19 1998-02-26 University Of Massachusetts Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire croisee d'especes
US6235970B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts, Amherst Campus CICM cells and non-human mammalian embryos prepared by nuclear transfer of a proliferating differentiated cell or its nucleus
US6395958B1 (en) * 1997-03-06 2002-05-28 Infigen, Inc. Method of producing a polypeptide in an ungulate
WO1999045100A1 (fr) * 1998-03-02 1999-09-10 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Lignees cellulaires embryonnaires ou de type souche produites par transplantation nucleaire d'especes croisees
US6258998B1 (en) * 1998-11-24 2001-07-10 Infigen, Inc. Method of cloning porcine animals
US20010037513A1 (en) * 2000-01-04 2001-11-01 Xiangzhong Yang Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE PUBMED [online] FIRST N.L. ET AL.: "Production of embryos by oocyte cytoplast-blastomere fusion in domstic animals", XP002952149, Database accession no. 1843343 *
DATABASE PUBMED [online] PRATHER R.S. ET AL.: "Nuclear transfer in mammalian embryos", XP002952148, accession no. NCBI Database accession no. 2406212 *
FULKA J. ET AL.: "Cloning by somatic cell molecular transfer", BIOESSAYS, vol. 20, no. 10, October 1998 (1998-10-01), pages 847 - 851, XP000889743 *
INT. REV. CYTOL., vol. 120, 1990, pages 169 - 190 *
J. REPROD. FERTIL. SUPPL., vol. 43, 1991, pages 245 - 254 *
STICE ET AL.: "Cloning: new breakthroughs leading to commercial opportunities", THERIOGENOLOGY, vol. 49, 1998, pages 129 - 138, XP002067444 *
WOLBE B.A. ET AL.: "Preimplantation development of embryos produced by intergeneric nuclear transplantation", THERIOGENOLOGY, vol. 33, no. 1, January 1990 (1990-01-01), pages 350, XP002952150 *

Also Published As

Publication number Publication date
AU2002250020A1 (en) 2002-08-19
WO2002062131A8 (fr) 2003-05-08
WO2002062131A2 (fr) 2002-08-15

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