WO2002061125A3 - Immobilised aptamers - Google Patents

Immobilised aptamers Download PDF

Info

Publication number
WO2002061125A3
WO2002061125A3 PCT/GB2002/000391 GB0200391W WO02061125A3 WO 2002061125 A3 WO2002061125 A3 WO 2002061125A3 GB 0200391 W GB0200391 W GB 0200391W WO 02061125 A3 WO02061125 A3 WO 02061125A3
Authority
WO
WIPO (PCT)
Prior art keywords
immobilised
aptamers
assays
find utility
find
Prior art date
Application number
PCT/GB2002/000391
Other languages
French (fr)
Other versions
WO2002061125A2 (en
Inventor
Abdessamad Tahiri-Alaoui
William Syward James
Jamal Ibrahim
Laura Frigotto
Original Assignee
Isis Innovation
Abdessamad Tahiri-Alaoui
William Syward James
Jamal Ibrahim
Laura Frigotto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0102272A external-priority patent/GB0102272D0/en
Priority claimed from GB0102271A external-priority patent/GB0102271D0/en
Priority claimed from GB0102270A external-priority patent/GB0102270D0/en
Priority claimed from GB0102273A external-priority patent/GB0102273D0/en
Application filed by Isis Innovation, Abdessamad Tahiri-Alaoui, William Syward James, Jamal Ibrahim, Laura Frigotto filed Critical Isis Innovation
Priority to AU2002226584A priority Critical patent/AU2002226584A1/en
Publication of WO2002061125A2 publication Critical patent/WO2002061125A2/en
Publication of WO2002061125A3 publication Critical patent/WO2002061125A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification

Abstract

Immobilised aptamers find utility in assays.
PCT/GB2002/000391 2001-01-29 2002-01-29 Immobilised aptamers WO2002061125A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002226584A AU2002226584A1 (en) 2001-01-29 2002-01-29 Immobilised aptamers

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GB0102272A GB0102272D0 (en) 2001-01-29 2001-01-29 Biligands
GB0102271A GB0102271D0 (en) 2001-01-29 2001-01-29 Streptavidin
GB0102270.6 2001-01-29
GB0102271.4 2001-01-29
GB0102270A GB0102270D0 (en) 2001-01-29 2001-01-29 Immobilisation
GB0102273A GB0102273D0 (en) 2001-01-29 2001-01-29 Hiv-1
GB0102272.2 2001-01-29
GB0102273.0 2001-01-29

Publications (2)

Publication Number Publication Date
WO2002061125A2 WO2002061125A2 (en) 2002-08-08
WO2002061125A3 true WO2002061125A3 (en) 2003-07-10

Family

ID=27447919

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/GB2002/000364 WO2002061079A2 (en) 2001-01-29 2002-01-29 Biligands
PCT/GB2002/000391 WO2002061125A2 (en) 2001-01-29 2002-01-29 Immobilised aptamers

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000364 WO2002061079A2 (en) 2001-01-29 2002-01-29 Biligands

Country Status (2)

Country Link
AU (2) AU2002226584A1 (en)
WO (2) WO2002061079A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2407825A1 (en) * 2002-10-11 2004-04-11 Andrew J. Simmonds Trap-tagging: a novel method for the identification and purification of rna-protein complexes
DE102004053918B4 (en) * 2004-11-05 2007-06-14 Analyticon Biotechnologies Ag Aptamer-based test system
EP2649183A1 (en) * 2010-12-10 2013-10-16 Merck Patent GmbH Bispecific aptamers mediating tumour cell lysis

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996004403A1 (en) * 1994-08-02 1996-02-15 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chimeric selex
WO1999060169A1 (en) * 1998-05-20 1999-11-25 Molecular Machines, Inc. Multimolecular devices, drug delivery systems and single-molecule selection
WO2000070329A1 (en) * 1999-05-14 2000-11-23 Brandeis University Nucleic acid-based detection
WO2001088123A1 (en) * 2000-05-18 2001-11-22 Isis Innovation Limited Ligands specific for an isoform of the prion protein

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1252099A (en) * 1997-11-26 1999-06-15 Medical Research Council Improved selex procedure and an anti-cd4 aptamer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996004403A1 (en) * 1994-08-02 1996-02-15 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chimeric selex
WO1999060169A1 (en) * 1998-05-20 1999-11-25 Molecular Machines, Inc. Multimolecular devices, drug delivery systems and single-molecule selection
WO2000070329A1 (en) * 1999-05-14 2000-11-23 Brandeis University Nucleic acid-based detection
WO2001088123A1 (en) * 2000-05-18 2001-11-22 Isis Innovation Limited Ligands specific for an isoform of the prion protein

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BACHLER MONIKA ET AL: "StreptoTag: A novel method for the isolation of RNA-binding proteins.", RNA (NEW YORK), vol. 5, no. 11, November 1999 (1999-11-01), pages 1509 - 1516, XP002237133, ISSN: 1355-8382 *
DAVIS ET AL: "STAINING OF CELL SURFACE HUMAN CD4 WITH 2'-F-PYRIMIDINE-CONTAINING RNA APTAMERS FOR FLOW CYTOMETRY", NUCLEIC ACIDS RESEARCH, vol. 26, no. 17, 1 September 1998 (1998-09-01), pages 3915 - 3924, XP002095387, ISSN: 0305-1048 *
SRISAWAT C ET AL: "STREPTAVIDIN APTAMERS: AFFINITY TAGS FOR THE STUDY OF RNAS AND RIBONUCLEOPROTEINS", RNA, vol. 7, no. 4, April 2001 (2001-04-01), pages 632 - 641, XP001120463, ISSN: 1355-8382 *
TAHIRI-ALAOUI ABDESSAMAD ET AL: "High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands.", NUCLEIC ACIDS RESEARCH, vol. 30, no. 10, 15 May 2002 (2002-05-15), pages e45, XP002237134, ISSN: 1362-4962 *

Also Published As

Publication number Publication date
WO2002061079A3 (en) 2003-09-04
WO2002061079A2 (en) 2002-08-08
AU2002226584A1 (en) 2002-08-12
AU2002226571A1 (en) 2002-08-12
WO2002061125A2 (en) 2002-08-08

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