WO2002061039A3 - Thuja plicata dirigent protein promotors - Google Patents

Thuja plicata dirigent protein promotors Download PDF

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Publication number
WO2002061039A3
WO2002061039A3 PCT/US2001/050631 US0150631W WO02061039A3 WO 2002061039 A3 WO2002061039 A3 WO 2002061039A3 US 0150631 W US0150631 W US 0150631W WO 02061039 A3 WO02061039 A3 WO 02061039A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
present
thuja plicata
dirigent protein
provides
Prior art date
Application number
PCT/US2001/050631
Other languages
French (fr)
Other versions
WO2002061039A2 (en
Inventor
Myoung K Kim
Jae-Heung Jeon
Laurence Davin
Norman G Lewis
Original Assignee
Univ Washington
Myoung K Kim
Jae-Heung Jeon
Laurence Davin
Norman G Lewis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Washington, Myoung K Kim, Jae-Heung Jeon, Laurence Davin, Norman G Lewis filed Critical Univ Washington
Priority to CA002426856A priority Critical patent/CA2426856A1/en
Publication of WO2002061039A2 publication Critical patent/WO2002061039A2/en
Publication of WO2002061039A3 publication Critical patent/WO2002061039A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Abstract

In one aspect the present invention provides seven, isolated, Thuja plicata genomic clones (SEQ ID NO:1; SEQ ID NO:3; SEQ ID NO:5; SEQ ID NO:7; SEQ ID NO:9; SEQ ID NO:11; and SEQ ID NO:13) that each comprise a dirigent protein gene. In another aspect, the present invention provides isolated promoter molecules that hybridize under conditions of 2 X SSC at 50 °C for 30 minutes to the complement of a promoter molecule consisting of a nucleic acid sequence selected from the group consisting of SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. In another aspect, the present invention provides methods of directing the expression of an open reading frame in a plant cell.
PCT/US2001/050631 2000-10-25 2001-10-25 Thuja plicata dirigent protein promotors WO2002061039A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002426856A CA2426856A1 (en) 2000-10-25 2001-10-25 Thuja plicata dirigent protein promoters

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US24332900P 2000-10-25 2000-10-25
US24333000P 2000-10-25 2000-10-25
US60/243,330 2000-10-25
US60/243,329 2000-10-25

Publications (2)

Publication Number Publication Date
WO2002061039A2 WO2002061039A2 (en) 2002-08-08
WO2002061039A3 true WO2002061039A3 (en) 2003-04-24

Family

ID=26935757

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050631 WO2002061039A2 (en) 2000-10-25 2001-10-25 Thuja plicata dirigent protein promotors

Country Status (2)

Country Link
CA (1) CA2426856A1 (en)
WO (1) WO2002061039A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0609216A2 (en) * 2005-03-21 2010-03-02 Pioneer Hi Bred Int Isolated nucleic acid molecule, vector construction, methods for obtaining transformed plant, to express a nucleotide sequence in a plant, in a plant cell, and preferably in a plant root and method for inductively expressing a nucleotide sequence in a vegetable root

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020113A1 (en) * 1996-11-08 1998-05-14 Washington State University Research Foundation Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use
US5773689A (en) * 1992-10-05 1998-06-30 North Carolina State University Method of increasing expression of foreign genes in plant cells
US6210942B1 (en) * 1996-11-08 2001-04-03 Washington State University Research Foundation Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use
US6380459B1 (en) * 1999-03-25 2002-04-30 Genesis Research & Development Corporation Ltd. Compositions and methods for the modification of gene expression

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5773689A (en) * 1992-10-05 1998-06-30 North Carolina State University Method of increasing expression of foreign genes in plant cells
WO1998020113A1 (en) * 1996-11-08 1998-05-14 Washington State University Research Foundation Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use
US6210942B1 (en) * 1996-11-08 2001-04-03 Washington State University Research Foundation Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use
US6380459B1 (en) * 1999-03-25 2002-04-30 Genesis Research & Development Corporation Ltd. Compositions and methods for the modification of gene expression

Also Published As

Publication number Publication date
CA2426856A1 (en) 2002-08-08
WO2002061039A2 (en) 2002-08-08

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