WO2002053750A3 - Inactivation of the polyphosphate kinase gene for microbial overproduction of antibiotics or enzymes - Google Patents

Inactivation of the polyphosphate kinase gene for microbial overproduction of antibiotics or enzymes Download PDF

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Publication number
WO2002053750A3
WO2002053750A3 PCT/FR2002/000026 FR0200026W WO02053750A3 WO 2002053750 A3 WO2002053750 A3 WO 2002053750A3 FR 0200026 W FR0200026 W FR 0200026W WO 02053750 A3 WO02053750 A3 WO 02053750A3
Authority
WO
WIPO (PCT)
Prior art keywords
overproduction
inactivation
antibiotics
microbial
enzymes
Prior art date
Application number
PCT/FR2002/000026
Other languages
French (fr)
Other versions
WO2002053750A2 (en
Inventor
Marie-Joelle Virolle
Hichem Chouayekh
Original Assignee
Centre Nat Rech Scient
Marie-Joelle Virolle
Hichem Chouayekh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Marie-Joelle Virolle, Hichem Chouayekh filed Critical Centre Nat Rech Scient
Priority to AU2002231854A priority Critical patent/AU2002231854A1/en
Publication of WO2002053750A2 publication Critical patent/WO2002053750A2/en
Publication of WO2002053750A3 publication Critical patent/WO2002053750A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Abstract

The invention concerns the use of micro-organism strains producing compounds of interest, whereof the ppk gene encoding polyphosphate kinase is inactivated, for implementing a method designed to stimulate the production of said compounds of interest by said transformed strains.
PCT/FR2002/000026 2001-01-04 2002-01-04 Inactivation of the polyphosphate kinase gene for microbial overproduction of antibiotics or enzymes WO2002053750A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002231854A AU2002231854A1 (en) 2001-01-04 2002-01-04 Inactivation of the polyphosphate kinase gene for microbial overproduction of antibiotics or enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0100088A FR2818991A1 (en) 2001-01-04 2001-01-04 STRAINS OF MICRO-ORGANISMS FOR THE IMPLEMENTATION OF A PROCESS FOR THE OVERPRODUCTION OF METABOLITES, IN PARTICULAR WITH ANTIBIOTIC OR ENZYMATIC ACTIVITY
FR01/00088 2001-01-04

Publications (2)

Publication Number Publication Date
WO2002053750A2 WO2002053750A2 (en) 2002-07-11
WO2002053750A3 true WO2002053750A3 (en) 2003-05-01

Family

ID=8858523

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/000026 WO2002053750A2 (en) 2001-01-04 2002-01-04 Inactivation of the polyphosphate kinase gene for microbial overproduction of antibiotics or enzymes

Country Status (3)

Country Link
AU (1) AU2002231854A1 (en)
FR (1) FR2818991A1 (en)
WO (1) WO2002053750A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2848567A1 (en) * 2002-12-16 2004-06-18 Centre Nat Rech Scient Using microorganisms with modified phoK/phoR operons for production of metabolites, e.g. antibiotics, provides increased yields by inducing deficiency of inorganic phosphate
CN100348727C (en) * 2005-03-08 2007-11-14 华中农业大学 Method for increasing yield of streptomycete antibiotic and the strain thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876987A (en) * 1996-02-07 1999-03-02 Board Of Trustees Operating Michigan State University Method, DNA and bacteria for hyperproduction of an antibiotic due to disruption of an AbsA gene

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876987A (en) * 1996-02-07 1999-03-02 Board Of Trustees Operating Michigan State University Method, DNA and bacteria for hyperproduction of an antibiotic due to disruption of an AbsA gene

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CROOKE ELLIOTT ET AL: "Genetically altered levels of inorganic polyphosphate in Escherichia coli.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 9, 1994, pages 6290 - 6295, XP002178097, ISSN: 0021-9258 *
DATABASE EMBL 20 April 2000 (2000-04-20), SAUNDERS D C ET AL: "Streptomyces coelicolor cosmid D84", XP002178098 *
FRALEY C D ET AL: "Inorganic polyphosphate mutants of bacterial pathogens are virulence-deficient.", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR, vol. 100, 2000, 100th General Meeting of the American Society for Microbiology;Los Angeles, California, USA; May 21-25, 2000, 2000, pages 111, XP008009629, ISSN: 1060-2011 *
KULAEV I S ET AL: "POSSIBLE ROLE OF HIGH MOLECULAR WEIGHT POLY PHOSPHATES IN THE BIOSYNTHESIS OF CHLORTETRACYCLINE IN STREPTOMYCES-AUREOFACIENS", BIOCHEMISTRY (ENGLISH TRANSLATION OF BIOKHIMIYA), vol. 41, no. 2 PART 2, 1976, pages 284 - 288, XP001024265, ISSN: 0006-2979 *
TINSLEY C R ET AL: "Cloning and Characterization of the Meningococcal Polyphosphate Kinase Gene: Production of Polyphosphate Synthesis Mutants.", INFECTION AND IMMUNITY, vol. 63, no. 5, 1995, pages 1624 - 1630, XP002218588, ISSN: 0019-9567 *
TZENG CHI-MENG ET AL: "Polyphosphate kinase is highly conserved in many bacterial pathogens.", MOLECULAR MICROBIOLOGY, vol. 29, no. 1, July 1998 (1998-07-01), pages 381 - 382, XP001024492, ISSN: 0950-382X *
TZENG CHI-MENG ET AL: "The multiple activities of polyphosphate kinase of Escherichia coli and their subunit structure determined by radiation target analysis.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 6, 11 February 2000 (2000-02-11), pages 3977 - 3983, XP002218587, ISSN: 0021-9258 *
ZYUZINA M L ET AL: "INTERRELATIONSHIP OF POLY PHOSPHATE METABOLISM WITH LEVORIN BIOSYNTHESIS IN STREPTOMYCES-LEVORIS", BIOCHEMISTRY (ENGLISH TRANSLATION OF BIOKHIMIYA), vol. 46, no. 5 PART 1, 1981, pages 642 - 646, XP001024268, ISSN: 0006-2979 *

Also Published As

Publication number Publication date
WO2002053750A2 (en) 2002-07-11
FR2818991A1 (en) 2002-07-05
AU2002231854A1 (en) 2002-07-16

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