WO2009009906A8 - Isoflavonoid production in plants - Google Patents

Isoflavonoid production in plants Download PDF

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Publication number
WO2009009906A8
WO2009009906A8 PCT/CA2008/001330 CA2008001330W WO2009009906A8 WO 2009009906 A8 WO2009009906 A8 WO 2009009906A8 CA 2008001330 W CA2008001330 W CA 2008001330W WO 2009009906 A8 WO2009009906 A8 WO 2009009906A8
Authority
WO
WIPO (PCT)
Prior art keywords
isoflavone
isoflavonoid
operatively linked
heterologous promoter
production plant
Prior art date
Application number
PCT/CA2008/001330
Other languages
French (fr)
Other versions
WO2009009906A1 (en
Inventor
Sangeeta Dhaubhadel
Original Assignee
Ca Minister Agriculture & Food
Sangeeta Dhaubhadel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Minister Agriculture & Food, Sangeeta Dhaubhadel filed Critical Ca Minister Agriculture & Food
Publication of WO2009009906A1 publication Critical patent/WO2009009906A1/en
Publication of WO2009009906A8 publication Critical patent/WO2009009906A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Abstract

A method for producing one or more than one isoflavonoid compound in a plant is described. The method comprises providing a production plant comprising one or more than one nucleotide sequence encoding isoflavone glycosyltransferase operatively linked to a heterologous promoter, isoflavone malonyltransferase operatively linked to a heterologous promoter, or both isoflavone glycosyltransferase operatively linked to a heterologous promoter and isoflavone malonyltransferase operatively linked to a heterologous promoter. The one or more than one nucleotide sequence is expressed in the production plant, to produce the one or more than one isoflavonoid compound. Preferably, the production plant contains all the other necessary enzymes of isoflavonoid biosynthesis to produce the one or more than one isoflavonoid compound.
PCT/CA2008/001330 2007-07-19 2008-07-16 Isoflavonoid production in plants WO2009009906A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96119307P 2007-07-19 2007-07-19
US60/961,193 2007-07-19

Publications (2)

Publication Number Publication Date
WO2009009906A1 WO2009009906A1 (en) 2009-01-22
WO2009009906A8 true WO2009009906A8 (en) 2009-04-02

Family

ID=40259272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2008/001330 WO2009009906A1 (en) 2007-07-19 2008-07-16 Isoflavonoid production in plants

Country Status (1)

Country Link
WO (1) WO2009009906A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101792779B (en) * 2010-02-10 2012-05-30 吉林农业大学 Method for producing isoflavones by fermenting lactic acid galactococcus engineering bacteria
EP2935607A1 (en) * 2012-12-21 2015-10-28 Evolva SA Anthocyanins with specific properties
CN107857786B (en) * 2017-12-14 2020-09-18 南京中医药大学 naringenin-7-O-glucoside-6' -succinate and application thereof in preparation of antibacterial or osteoporosis treatment medicines
CN109971808B (en) * 2017-12-28 2020-09-18 北京中医药大学 Process for preparing malonylated products

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060135758A1 (en) * 2004-08-31 2006-06-22 Kunsheng Wu Soybean polymorphisms and methods of genotyping

Also Published As

Publication number Publication date
WO2009009906A1 (en) 2009-01-22

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