WO2002050104A3 - Methodes et cellules de detection de modulateurs de proteines rgs - Google Patents

Methodes et cellules de detection de modulateurs de proteines rgs Download PDF

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Publication number
WO2002050104A3
WO2002050104A3 PCT/US2001/045105 US0145105W WO0250104A3 WO 2002050104 A3 WO2002050104 A3 WO 2002050104A3 US 0145105 W US0145105 W US 0145105W WO 0250104 A3 WO0250104 A3 WO 0250104A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
methods
pheromone
rgs proteins
detecting
Prior art date
Application number
PCT/US2001/045105
Other languages
English (en)
Other versions
WO2002050104A2 (fr
WO2002050104A9 (fr
Inventor
Kathleen H Young
Jian Cao
David Sheuy
Original Assignee
Wyeth Corp
Kathleen H Young
Jian Cao
David Sheuy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp, Kathleen H Young, Jian Cao, David Sheuy filed Critical Wyeth Corp
Priority to AU2002243255A priority Critical patent/AU2002243255A1/en
Priority to IL15619901A priority patent/IL156199A0/xx
Priority to KR10-2003-7007356A priority patent/KR20030081337A/ko
Priority to JP2002551997A priority patent/JP2004520824A/ja
Priority to EP01989139A priority patent/EP1339740A2/fr
Priority to CA002430475A priority patent/CA2430475A1/fr
Priority to MXPA03004700A priority patent/MXPA03004700A/es
Publication of WO2002050104A2 publication Critical patent/WO2002050104A2/fr
Publication of WO2002050104A9 publication Critical patent/WO2002050104A9/fr
Publication of WO2002050104A3 publication Critical patent/WO2002050104A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne de nouvelles cellules sensibles à une phéromone comprenant un acide nucléique hétérologue codant pour un rapporteur lié à un promoteur sensible aux phéromones. Lesdites cellules peuvent également comprendre un acide nucléique hétérologue codant pour une protéine RGS. L'invention concerne également des méthodes consistant à utiliser ces cellules pour détecter la capacité d'un échantillon d'essai à modifier l'expression du gène rapporteur associée à une protéine RGS.
PCT/US2001/045105 2000-12-01 2001-12-03 Methodes et cellules de detection de modulateurs de proteines rgs WO2002050104A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2002243255A AU2002243255A1 (en) 2000-12-01 2001-12-03 Methods and cells for detecting modulators of RGS proteins
IL15619901A IL156199A0 (en) 2000-12-01 2001-12-03 Methods and cells for detecting modulators of rgs proteins
KR10-2003-7007356A KR20030081337A (ko) 2000-12-01 2001-12-03 Ｒｇｓ 단백질의 모듈레이터 검출용 방법 및 세포
JP2002551997A JP2004520824A (ja) 2000-12-01 2001-12-03 Ｒｇｓタンパク質のモジュレーターを検出するための方法および細胞
EP01989139A EP1339740A2 (fr) 2000-12-01 2001-12-03 Methodes et cellules de detection de modulateurs de proteines rgs
CA002430475A CA2430475A1 (fr) 2000-12-01 2001-12-03 Methodes et cellules de detection de modulateurs de proteines rgs
MXPA03004700A MXPA03004700A (es) 2000-12-01 2001-12-03 Metodos y celulas para detectar moduladores de proteinas rgs.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25014700P 2000-12-01 2000-12-01
US60/250,147 2000-12-01

Publications (3)

Publication Number Publication Date
WO2002050104A2 WO2002050104A2 (fr) 2002-06-27
WO2002050104A9 WO2002050104A9 (fr) 2002-12-19
WO2002050104A3 true WO2002050104A3 (fr) 2003-04-10

Family

ID=22946480

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/045105 WO2002050104A2 (fr) 2000-12-01 2001-12-03 Methodes et cellules de detection de modulateurs de proteines rgs

Country Status (9)

Country Link
EP (1) EP1339740A2 (fr)
JP (1) JP2004520824A (fr)
KR (1) KR20030081337A (fr)
CN (1) CN1478097A (fr)
AU (1) AU2002243255A1 (fr)
CA (1) CA2430475A1 (fr)
IL (1) IL156199A0 (fr)
MX (1) MXPA03004700A (fr)
WO (1) WO2002050104A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846671B2 (en) 2002-01-28 2010-12-07 Bristol-Myers Squibb Company Methods of screening for agents that modulate the interaction of RGS and Gαq and urinary incontinence
KR100968640B1 (ko) * 2007-10-06 2010-07-06 재단법인서울대학교산학협력재단 단일세포의 신호분석방법
EP3253779A1 (fr) * 2015-02-06 2017-12-13 Cargill, Incorporated Enzymes glucoamylases modifiées et souches de levures caractérisées par une production de bioproduits améliorée
CN105126079B (zh) * 2015-09-29 2019-03-01 武汉大学 G蛋白信号转导调节蛋白14（rgs14）在治疗心肌肥厚中的功能及应用
CN105106936B (zh) * 2015-09-29 2018-05-15 武汉大学 G蛋白信号转导调节蛋白10（rgs10）在治疗心肌肥厚中的功能及应用
CA3032736A1 (fr) 2016-08-05 2018-02-08 Cargill, Incorporated Polypeptides de glucoamylase modifies par une sequence de tete et souches de levure manipulees presentant une production de produits biologiques amelioree

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998013513A2 (fr) * 1996-09-24 1998-04-02 Cadus Pharmaceutical Corporation Procedes et compositions pour identifier des modulateurs de recepteur
WO1999058670A1 (fr) * 1998-05-08 1999-11-18 Cadus Pharmaceutical Corporation Molecules d'acides nucleiques et proteines ags, et utilisations correspondantes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998013513A2 (fr) * 1996-09-24 1998-04-02 Cadus Pharmaceutical Corporation Procedes et compositions pour identifier des modulateurs de recepteur
WO1999058670A1 (fr) * 1998-05-08 1999-11-18 Cadus Pharmaceutical Corporation Molecules d'acides nucleiques et proteines ags, et utilisations correspondantes

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CORMACK B P ET AL: "YEAST-ENHANCED GREEN FLUORESCENT PROTEIN (YEGFP): A REPORTER OF GENE EXPRESSION IN CANDIDA ALBICANS", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 143, no. PART 2, 1 February 1997 (1997-02-01), pages 303 - 311, XP002073530, ISSN: 1350-0872 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 11 November 2001 (2001-11-11), ZHANG J ET AL: "Modulation of RGS (regulators of G-protein signaling) proteins.", XP002223601, Database accession no. PREV200100481761 *
DEL POETA MAURIZIO ET AL: "Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein.", INFECTION AND IMMUNITY, vol. 67, no. 4, April 1999 (1999-04-01), pages 1812 - 1820, XP002223587, ISSN: 0019-9567 *
DRUEY KIRK M ET AL: "Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family.", NATURE (LONDON), vol. 379, no. 6567, 1996, pages 742 - 746, XP002929721, ISSN: 0028-0836 *
MCENTAFFER RANDALL L ET AL: "Modulation of transducin GTPase activity by chimeric RGS16 and RGS9 regulators of G protein signaling and the effector molecule.", BIOCHEMISTRY, vol. 38, no. 16, 20 April 1999 (1999-04-20), pages 4931 - 4937, XP002223590, ISSN: 0006-2960 *
PANETTA ROSEMARIE ET AL: "Regulators of G-protein signaling (RGS) 1 and 16 are induced in response to bacterial lipopolysaccharide and stimulate c-fos promoter expression.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 259, no. 3, 16 June 1999 (1999-06-16), pages 550 - 556, XP002223591, ISSN: 0006-291X *
SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 27, no. 1, 2001, 31st Annual Meeting of the Society for Neuroscience;San Diego, California, USA; November 10-15, 2001, pages 108, ISSN: 0190-5295 *
VIEITES J M ET AL: "EXPRESSION AND IN VIVO DETERMINATION OF FIREFLY LUCIFERASE AS GENE REPORTER IN SACCHAROMYCES CEREVISIAE", YEAST, CHICHESTER, SUSSEX, GB, vol. 10, no. 10, 1994, pages 1321 - 1327, XP000915834, ISSN: 0749-503X *
WATSON PETER ET AL: "An RGS protein regulates the pheromone response in the fission yeast Schizosaccharomyces pombe.", MOLECULAR MICROBIOLOGY, vol. 33, no. 3, August 1999 (1999-08-01), pages 623 - 634, XP002223588, ISSN: 0950-382X *

Also Published As

Publication number Publication date
WO2002050104A2 (fr) 2002-06-27
MXPA03004700A (es) 2003-08-19
WO2002050104A9 (fr) 2002-12-19
CA2430475A1 (fr) 2002-06-27
EP1339740A2 (fr) 2003-09-03
AU2002243255A1 (en) 2002-07-01
JP2004520824A (ja) 2004-07-15
KR20030081337A (ko) 2003-10-17
CN1478097A (zh) 2004-02-25
IL156199A0 (en) 2003-12-23

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