WO2002034936A3 - Novel s. pyogenes dna polymerase iii holoenzyme nucleic acid molecules and proteins - Google Patents

Novel s. pyogenes dna polymerase iii holoenzyme nucleic acid molecules and proteins Download PDF

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Publication number
WO2002034936A3
WO2002034936A3 PCT/US2001/048396 US0148396W WO0234936A3 WO 2002034936 A3 WO2002034936 A3 WO 2002034936A3 US 0148396 W US0148396 W US 0148396W WO 0234936 A3 WO0234936 A3 WO 0234936A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
proteins
novel
dna polymerase
Prior art date
Application number
PCT/US2001/048396
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French (fr)
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WO2002034936A9 (en
WO2002034936A2 (en
Inventor
Charles S Mchenry
James M Bullard
Nebojsa Janjic
Erika L Manhardt
Vladimir Kery
Jennifer C Williams
Original Assignee
Replidyne Inc
Charles S Mchenry
James M Bullard
Nebojsa Janjic
Erika L Manhardt
Vladimir Kery
Jennifer C Williams
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Replidyne Inc, Charles S Mchenry, James M Bullard, Nebojsa Janjic, Erika L Manhardt, Vladimir Kery, Jennifer C Williams filed Critical Replidyne Inc
Priority to AU2002232586A priority Critical patent/AU2002232586A1/en
Publication of WO2002034936A2 publication Critical patent/WO2002034936A2/en
Publication of WO2002034936A3 publication Critical patent/WO2002034936A3/en
Publication of WO2002034936A9 publication Critical patent/WO2002034936A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

S. pyogenes nucleic acid molecules encoding polC, dnaE, holA, holB, dnaX, dnaN, SSB, dnaG, dnaQ, dnaA and dnaB proteins, as well as nucleic acid molecules comprising oriC are provided. The encoded proteins are also provided. The nucleic acid molecules and proteins are useful for reconstituting replicases and polymerases for sequencing, amplification, and screening for compounds which modulate the function of the polymersase or replicase.
PCT/US2001/048396 2000-10-27 2001-10-29 Novel s. pyogenes dna polymerase iii holoenzyme nucleic acid molecules and proteins WO2002034936A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002232586A AU2002232586A1 (en) 2000-10-27 2001-10-29 Novel s. pyogenes dna polymerase iii holoenzyme nucleic acid molecules and proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24402300P 2000-10-27 2000-10-27
US60/244,023 2000-10-27

Publications (3)

Publication Number Publication Date
WO2002034936A2 WO2002034936A2 (en) 2002-05-02
WO2002034936A3 true WO2002034936A3 (en) 2002-07-25
WO2002034936A9 WO2002034936A9 (en) 2003-04-17

Family

ID=22921084

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/048396 WO2002034936A2 (en) 2000-10-27 2001-10-29 Novel s. pyogenes dna polymerase iii holoenzyme nucleic acid molecules and proteins

Country Status (2)

Country Link
AU (1) AU2002232586A1 (en)
WO (1) WO2002034936A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
US8431376B2 (en) 2004-04-26 2013-04-30 The Regents Of The University Of Colorado Bacterial replication systems and methods

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001009164A2 (en) * 1999-07-29 2001-02-08 The Rockefeller University Dna replication proteins of gram positive bacteria and their use to screen for chemical inhibitors

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001009164A2 (en) * 1999-07-29 2001-02-08 The Rockefeller University Dna replication proteins of gram positive bacteria and their use to screen for chemical inhibitors

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRUCK ET AL.: "The DNA replication machine of a gram-positive organism", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 37, 15 September 2000 (2000-09-15), pages 28971 - 28983, XP002909745 *
DATABASE GENEMBL [online] 10 August 2000 (2000-08-10), BRUCK ET AL.: "The DNA replication machine of a gram positive organism", XP002909746, Database accession no. AF280761 *

Also Published As

Publication number Publication date
WO2002034936A9 (en) 2003-04-17
WO2002034936A2 (en) 2002-05-02
AU2002232586A1 (en) 2002-05-06

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