WO2002034908A3 - Method for temporally controlling antisense-mediated gene inactivation - Google Patents

Method for temporally controlling antisense-mediated gene inactivation Download PDF

Info

Publication number
WO2002034908A3
WO2002034908A3 PCT/EP2001/012248 EP0112248W WO0234908A3 WO 2002034908 A3 WO2002034908 A3 WO 2002034908A3 EP 0112248 W EP0112248 W EP 0112248W WO 0234908 A3 WO0234908 A3 WO 0234908A3
Authority
WO
WIPO (PCT)
Prior art keywords
mediated gene
genes
gene inactivation
temporally controlling
relates
Prior art date
Application number
PCT/EP2001/012248
Other languages
French (fr)
Other versions
WO2002034908A2 (en
Inventor
Alexander D Crawford
Camilla V Esguerra
Original Assignee
Mermaid Pharmaceuticals Gmbh
Alexander D Crawford
Camilla V Esguerra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mermaid Pharmaceuticals Gmbh, Alexander D Crawford, Camilla V Esguerra filed Critical Mermaid Pharmaceuticals Gmbh
Priority to AU2002224787A priority Critical patent/AU2002224787A1/en
Publication of WO2002034908A2 publication Critical patent/WO2002034908A2/en
Publication of WO2002034908A3 publication Critical patent/WO2002034908A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/13—Decoys
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C12N2310/3181—Peptide nucleic acid, PNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3233—Morpholino-type ring

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a novel method for inactivating genes in model organisms in a temporally specific manner. In particular, the invention relates to the delay of antisensemediated inhibition of gene expression by temporarily blocking the transcript-binding activity of antisense oligomers, which facilitates the stage-specific inactivation of individual genes and enables the functional analysis of genes with multiple functional roles in vertebrate development.
PCT/EP2001/012248 2000-10-23 2001-10-23 Method for temporally controlling antisense-mediated gene inactivation WO2002034908A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002224787A AU2002224787A1 (en) 2000-10-23 2001-10-23 Method for temporally controlling antisense-mediated gene inactivation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24252000P 2000-10-23 2000-10-23
US60/242,520 2000-10-23

Publications (2)

Publication Number Publication Date
WO2002034908A2 WO2002034908A2 (en) 2002-05-02
WO2002034908A3 true WO2002034908A3 (en) 2002-07-11

Family

ID=22915094

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/012248 WO2002034908A2 (en) 2000-10-23 2001-10-23 Method for temporally controlling antisense-mediated gene inactivation

Country Status (2)

Country Link
AU (1) AU2002224787A1 (en)
WO (1) WO2002034908A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10252806A1 (en) * 2002-11-13 2004-06-03 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung Use of fish larvae as a screening model
CN106070063B (en) * 2016-07-07 2019-03-05 贵州医科大学 Transgenic zebrafish system and its method for building up with abcb4
CA3142526A1 (en) * 2019-06-03 2020-12-10 Quralis Corporation Oligonucleotides and methods of use for treating neurological diseases

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994015646A1 (en) * 1993-01-07 1994-07-21 Thomas Jefferson University Antisense inhibition of c-myc to modulate the proliferation of smooth muscle cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994015646A1 (en) * 1993-01-07 1994-07-21 Thomas Jefferson University Antisense inhibition of c-myc to modulate the proliferation of smooth muscle cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
HEASMAN JANET ET AL: "beta-catenin signaling activity dissected in the early Xenopus embryo: A novel antisense approach.", DEVELOPMENTAL BIOLOGY, vol. 222, no. 1, 1 June 2000 (2000-06-01), pages 124 - 134, XP002196303, ISSN: 0012-1606 *
HEASMAN JANET: "Morpholino oligos: making sense of antisense?", DEVELOPMENTAL BIOLOGY, vol. 243, - 15 March 2002 (2002-03-15), pages 209 - 214, XP002196308 *
HERBERT J M ET AL: "Intimal hyperplasia following vascular injury is not inhibited by an antisense thrombin receptor oligodeoxynucleotide.", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 170, no. 2, 1997, pages 106 - 114, XP002196306, ISSN: 0021-9541 *
HERTL MICHAEL ET AL: "Inhibition of interferon-gamma-induced intercellular adhesion molecule-1 expression on human keratinocytes by phosphorothioate antisense oligodeoxynucleotides is the consequence of antisense-specific and antisense-non-specific effects.", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 104, no. 5, 1995, pages 813 - 818, XP002196305, ISSN: 0022-202X *
NASEVICIUS AIDAS ET AL: "Effective targeted gene 'knockdown' in zebrafish.", NATURE GENETICS, vol. 26, no. 2, October 2000 (2000-10-01), pages 216 - 220, XP002196304, ISSN: 1061-4036 *
RAATS JOS M H ET AL: "Modified mRNA rescue of maternal CK1/8 mRNA depletion in Xenopus oocytes.", ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, vol. 7, no. 4, 1997, pages 263 - 277, XP002196307, ISSN: 1087-2906 *

Also Published As

Publication number Publication date
AU2002224787A1 (en) 2002-05-06
WO2002034908A2 (en) 2002-05-02

Similar Documents

Publication Publication Date Title
WO2003093441A3 (en) A method of regulating gene expression
AU5982300A (en) Novel methods and medicament for treating infectious diseases involving microbial biofilms
EP1587574A4 (en) SELECTIVE 11b-HSD INHIBITORS AND METHODS FOR USE THEREOF
WO1999051731A3 (en) Maize histone deacetylases and their use
EP1189918A4 (en) Antisense modulation of integrin beta 3 expression
AU2003287254A1 (en) Inhibition of gene expression using rna interfering agents
AU2003302458A8 (en) Liquid containing diatom, diatom and method for culturing diatom
ZA200201874B (en) Plant regulatory sequences for control of gene expression.
MXPA02003232A (en) Compositions and methods for altering gene expression.
WO2002034908A3 (en) Method for temporally controlling antisense-mediated gene inactivation
WO2001083513A3 (en) Antisense modulation of inhibitor of dna binding-1 expression
AU2180501A (en) Novel DNA polymerase inhibitors, mikanolide and dihydromikanolide
AU2001270244A1 (en) Plant regulatory sequences for selective control of gene expression
AU2003272968A8 (en) Tyrosinase activity controlling agents, process for producing the same and external preparations containing the same
WO2004003152A3 (en) Sos1 inhibitors
FR2791360B1 (en) INDUCTIBLE PROMOTER, COMTII, CHIMERIC GENE COMPRISING SAME AND TRANSFORMED PLANTS
WO2002046367A3 (en) Antisense modulation of cellular apoptosis susceptibility gene expression
WO2003062197A3 (en) Nek2 inhibitors
AU2001292310A1 (en) Method of regulating the activity of expression product of gene transferred intoliving body
AU2001257385A1 (en) Plant regulatory sequences for selective control of gene expression
WO2004001061A3 (en) Antisense modulation of heme oxygenase 1 expression
AU2001225484A1 (en) Agents correcting gene expression regulatory error
WO2002010376A1 (en) HUMAN II TYPE 5α-REDUCTASE PROMOTER GENE AND USE THEREOF
EP1551383A4 (en) Methods for preventing and treating microbial infections by modulating transcription factors
AU2001271111A1 (en) Human prothrombin kringle with endothelial cell growth inhibitory activity, vector and host thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 69(1) EPC (EPO FORM 1205A DATED 16.07.2003)

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP