WO2002034040A3 - Method for generating transgenic fish embryos using an episomal vector system - Google Patents
Method for generating transgenic fish embryos using an episomal vector system Download PDFInfo
- Publication number
- WO2002034040A3 WO2002034040A3 PCT/EP2001/012246 EP0112246W WO0234040A3 WO 2002034040 A3 WO2002034040 A3 WO 2002034040A3 EP 0112246 W EP0112246 W EP 0112246W WO 0234040 A3 WO0234040 A3 WO 0234040A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- vector system
- episomal vector
- fish embryos
- generating transgenic
- transgenic fish
- Prior art date
Links
- 241000251468 Actinopterygii Species 0.000 title abstract 3
- 210000002257 embryonic structure Anatomy 0.000 title abstract 3
- 230000009261 transgenic effect Effects 0.000 title abstract 2
- 108700019146 Transgenes Proteins 0.000 abstract 1
- 230000010502 episomal replication Effects 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/40—Fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Environmental Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2002221731A AU2002221731A1 (en) | 2000-10-23 | 2001-10-23 | Method for generating transgenic fish embryos using an episomal vector system |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US24249900P | 2000-10-23 | 2000-10-23 | |
US60/242,499 | 2000-10-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002034040A2 WO2002034040A2 (en) | 2002-05-02 |
WO2002034040A3 true WO2002034040A3 (en) | 2002-11-28 |
Family
ID=22915010
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2001/012246 WO2002034040A2 (en) | 2000-10-23 | 2001-10-23 | Method for generating transgenic fish embryos using an episomal vector system |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU2002221731A1 (en) |
WO (1) | WO2002034040A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10252806A1 (en) * | 2002-11-13 | 2004-06-03 | DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung | Use of fish larvae as a screening model |
EP1943345B1 (en) | 2005-10-05 | 2011-04-06 | Università Degli Studi Di Milano - Bicocca | A method for the transfer of episomal vectors into animal cells |
WO2024151877A2 (en) * | 2023-01-11 | 2024-07-18 | Engage Biologics Inc. | Non-viral expression systems and methods of use thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998056902A2 (en) * | 1997-06-09 | 1998-12-17 | Medical College Of Georgia Research Institute, Inc. | Transgenic fish with tissue-specific expression |
-
2001
- 2001-10-23 WO PCT/EP2001/012246 patent/WO2002034040A2/en active Application Filing
- 2001-10-23 AU AU2002221731A patent/AU2002221731A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998056902A2 (en) * | 1997-06-09 | 1998-12-17 | Medical College Of Georgia Research Institute, Inc. | Transgenic fish with tissue-specific expression |
Non-Patent Citations (3)
Title |
---|
ELBRECHT A ET AL: "EPISOMAL MAINTENANCE OF A BOVINE PAPILLOMA VIRUS VECTOR IN TRANSGENIC MICE", MOLECULAR AND CELLULAR BIOLOGY, WASHINGTON, DC, US, vol. 7, no. 3, 1 March 1987 (1987-03-01), pages 1276 - 1279, XP000673609, ISSN: 0270-7306 * |
LINNEY E ET AL: "Transgene expression in zebrafish: A comparison of retroviral-vector and DNA-injection approaches.", DEVELOPMENTAL BIOLOGY. UNITED STATES 1 SEP 1999, vol. 213, no. 1, 1 September 1999 (1999-09-01), pages 207 - 216, XP001050547, ISSN: 0012-1606 * |
WADE-MARTINS RICHARD ET AL: "Long-term stability of large insert genomic DNA episomal shuttle vectors in human cells.", NUCLEIC ACIDS RESEARCH, vol. 27, no. 7, 1 April 1999 (1999-04-01), pages 1674 - 1682, XP002204660, ISSN: 0305-1048 * |
Also Published As
Publication number | Publication date |
---|---|
WO2002034040A2 (en) | 2002-05-02 |
AU2002221731A1 (en) | 2002-05-06 |
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