WO2000025578A3 - Methods for cloning animals - Google Patents

Methods for cloning animals Download PDF

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Publication number
WO2000025578A3
WO2000025578A3 PCT/US1999/025786 US9925786W WO0025578A3 WO 2000025578 A3 WO2000025578 A3 WO 2000025578A3 US 9925786 W US9925786 W US 9925786W WO 0025578 A3 WO0025578 A3 WO 0025578A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
animal
oocyte
cloning
nuclear transfer
Prior art date
Application number
PCT/US1999/025786
Other languages
French (fr)
Other versions
WO2000025578A2 (en
Inventor
Alexander B Baguisi
Eric W Overstrom
Original Assignee
Tufts College
Alexander B Baguisi
Eric W Overstrom
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tufts College, Alexander B Baguisi, Eric W Overstrom filed Critical Tufts College
Priority to DE69935607T priority Critical patent/DE69935607T2/en
Priority to EP99962678A priority patent/EP1127112B1/en
Priority to JP2000579047A priority patent/JP2003523719A/en
Publication of WO2000025578A2 publication Critical patent/WO2000025578A2/en
Publication of WO2000025578A3 publication Critical patent/WO2000025578A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8772—Caprine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0273—Cloned animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Humanized animals, e.g. knockin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/102—Caprine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
PCT/US1999/025786 1998-11-02 1999-11-02 Methods for cloning animals WO2000025578A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE69935607T DE69935607T2 (en) 1998-11-02 1999-11-02 METHOD FOR CLONING ANIMALS
EP99962678A EP1127112B1 (en) 1998-11-02 1999-11-02 Methods for cloning animals
JP2000579047A JP2003523719A (en) 1998-11-02 1999-11-02 How to clone animals

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US13106199P 1999-04-26 1999-04-26
US13132899P 1999-04-26 1999-04-26
US60/131,061 1999-04-26
US60/131,328 1999-04-26
US14931799P 1999-08-17 1999-08-17
US60/149,317 1999-08-17

Publications (2)

Publication Number Publication Date
WO2000025578A2 WO2000025578A2 (en) 2000-05-11
WO2000025578A3 true WO2000025578A3 (en) 2000-11-16

Family

ID=27384090

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/025786 WO2000025578A2 (en) 1998-11-02 1999-11-02 Methods for cloning animals

Country Status (1)

Country Link
WO (1) WO2000025578A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
EP1375654A3 (en) * 1998-11-02 2008-01-16 GTC Biotherapeutics, Inc. Transgenic and cloned mammals
WO2002019811A2 (en) * 2000-09-06 2002-03-14 Nexia Biotechnologies, Inc. Generation of transgenic animals using nuclear transfer and oocytes at the germinal vesicle stage
EP1370132A2 (en) 2001-03-21 2003-12-17 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
WO2006018316A1 (en) * 2004-08-20 2006-02-23 Ingenium Pharmaceuticals Ag Methods for the production of improved live stocks and disease models for therapeutic research
US20100205678A1 (en) * 2007-01-05 2010-08-12 Overstrom Eric W Oocyte spindle-associated factors improve somatic cell cloning

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003398A1 (en) * 1993-07-23 1995-02-02 Monash University Enucleation of oocytes
WO1996026268A1 (en) * 1995-02-21 1996-08-29 Genzyme Transgenics Corporation Transgenically produced antithrombin iii
WO1998029532A1 (en) * 1997-01-02 1998-07-09 Monash University A method of oocyte enucleation and production of reconstituted embryos
WO1999001163A1 (en) * 1997-07-03 1999-01-14 University Of Massachusetts Cloning using donor nuclei from non-serum starved, differentiated cells
US5952222A (en) * 1995-08-04 1999-09-14 The Board Of Trustees Of The University Of Arkansas Functional enucleation of bovine oocytes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003398A1 (en) * 1993-07-23 1995-02-02 Monash University Enucleation of oocytes
WO1996026268A1 (en) * 1995-02-21 1996-08-29 Genzyme Transgenics Corporation Transgenically produced antithrombin iii
US5952222A (en) * 1995-08-04 1999-09-14 The Board Of Trustees Of The University Of Arkansas Functional enucleation of bovine oocytes
WO1998029532A1 (en) * 1997-01-02 1998-07-09 Monash University A method of oocyte enucleation and production of reconstituted embryos
WO1999001163A1 (en) * 1997-07-03 1999-01-14 University Of Massachusetts Cloning using donor nuclei from non-serum starved, differentiated cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BAGUISI A, ET AL.: "Production of goats by somatic cell nuclear transfer.", NAT BIOTECHNOL, vol. 17, no. 5, May 1999 (1999-05-01), pages 456 - 461, XP000891364 *
BORDIGNON V ET AL: "TELOPHASE ENUCLEATION: AN IMPROVED METHOD TO PREPARE RECIPIENT CYTOPLASTS FOR USE IN BOVINE NUCLEAR TRANSFER", MOLECULAR REPRODUCTION AND DEVELOPMENT,US,NEW YORK, NY, vol. 49, January 1998 (1998-01-01), pages 29 - 36, XP000910821 *
CIBELLI J B ET AL: "CLONED TRANSGENIC CALVES PRODUCED FROM NONQUIESCENT FETAL FIBROBLASTS", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 280, 22 May 1998 (1998-05-22), pages 1256 - 1258, XP000786003, ISSN: 0036-8075 *
ROBL J M ET AL: "SOMATIC CELL NUCLEAR TRANSPLANTATION IN CATTLE", ANNUAL MEETING SOC. STUDY REPROD,, vol. 58, 8 August 1998 (1998-08-08), pages 25, XP000791151 *

Also Published As

Publication number Publication date
WO2000025578A2 (en) 2000-05-11

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