WO2002031204A3 - Assay to detect integration of a retrovirus polynucleotide into a target nucleic acid - Google Patents

Assay to detect integration of a retrovirus polynucleotide into a target nucleic acid Download PDF

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Publication number
WO2002031204A3
WO2002031204A3 PCT/US2001/031807 US0131807W WO0231204A3 WO 2002031204 A3 WO2002031204 A3 WO 2002031204A3 US 0131807 W US0131807 W US 0131807W WO 0231204 A3 WO0231204 A3 WO 0231204A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
assay
integration
retrovirus
Prior art date
Application number
PCT/US2001/031807
Other languages
French (fr)
Other versions
WO2002031204A2 (en
Inventor
Richard S Kornbluth
Alexei Brooun
Original Assignee
Univ California San Diego
Richard S Kornbluth
Alexei Brooun
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California San Diego, Richard S Kornbluth, Alexei Brooun filed Critical Univ California San Diego
Priority to CA002424998A priority Critical patent/CA2424998A1/en
Priority to AU2001296814A priority patent/AU2001296814A1/en
Priority to EP01977718A priority patent/EP1349959A2/en
Publication of WO2002031204A2 publication Critical patent/WO2002031204A2/en
Publication of WO2002031204A3 publication Critical patent/WO2002031204A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • AIDS & HIV (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods for detecting integration of a polynucleotide such as a retrovirus into a target nucleic acid molecule are provided, as are methods of identifying cellular factors involved in such an integration event and of identifying agents that can modulate such integration events. Also provided are reagents and kits for performing such methods.
PCT/US2001/031807 2000-10-10 2001-10-09 Assay to detect integration of a retrovirus polynucleotide into a target nucleic acid WO2002031204A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002424998A CA2424998A1 (en) 2000-10-10 2001-10-09 Nucleic acid integration assay
AU2001296814A AU2001296814A1 (en) 2000-10-10 2001-10-09 Assay to detect integration of a retrovirus polynucleotide into a target nucleic acid
EP01977718A EP1349959A2 (en) 2000-10-10 2001-10-09 Assay to detect integration of a retrovirus polynucleotide into a target nucleic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23931300P 2000-10-10 2000-10-10
US60/239,313 2000-10-10

Publications (2)

Publication Number Publication Date
WO2002031204A2 WO2002031204A2 (en) 2002-04-18
WO2002031204A3 true WO2002031204A3 (en) 2003-08-07

Family

ID=22901615

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/031807 WO2002031204A2 (en) 2000-10-10 2001-10-09 Assay to detect integration of a retrovirus polynucleotide into a target nucleic acid

Country Status (4)

Country Link
EP (1) EP1349959A2 (en)
AU (1) AU2001296814A1 (en)
CA (1) CA2424998A1 (en)
WO (1) WO2002031204A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020813A1 (en) * 1991-05-17 1992-11-26 Dana Farber Cancer Institute Assays for factors affecting circularization of dna, assays for factors affecting dna integration, factors, and uses thereof
WO1999047660A1 (en) * 1998-03-18 1999-09-23 The Salk Institute For Biological Studies Retroviral packaging cell line

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020813A1 (en) * 1991-05-17 1992-11-26 Dana Farber Cancer Institute Assays for factors affecting circularization of dna, assays for factors affecting dna integration, factors, and uses thereof
WO1999047660A1 (en) * 1998-03-18 1999-09-23 The Salk Institute For Biological Studies Retroviral packaging cell line

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HANSEN M S ET AL: "Integration complexes derived from HIV vectors for rapid assays in vitro.", NATURE BIOTECHNOLOGY. UNITED STATES JUN 1999, vol. 17, no. 6, June 1999 (1999-06-01), pages 578 - 582, XP001097540, ISSN: 1087-0156 *
KORNBLUTH R S ET AL: "Quantitative analysis of HIV-1 pre-integration complexes using an in vitro, PCR-based integration assay.", FASEB JOURNAL, vol. 10, no. 6, 1996, Joint Meeting of the American Society for Biochemistry and Molecular Biology, the American Society for Investigative Pathology and the American Association of Immunologists;New Orleans, Louisiana, USA, pages A1061, XP009003098, ISSN: 0892-6638 *
LU F ET AL: "Tn916 target DNA sequences bind the C-terminal domain of integrase protein with different affinities that correlate with transposon insertion frequency.", JOURNAL OF BACTERIOLOGY. UNITED STATES APR 1995, vol. 177, no. 8, April 1995 (1995-04-01), pages 1938 - 1946, XP002226005, ISSN: 0021-9193 *
PRUSS D ET AL: "Human immunodeficiency virus integrase directs integration to sites of severe DNA distortion within the nucleosome core.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. UNITED STATES 21 JUN 1994, vol. 91, no. 13, 21 June 1994 (1994-06-21), pages 5913 - 5917, XP002226004, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU2001296814A1 (en) 2002-04-22
CA2424998A1 (en) 2002-04-18
WO2002031204A2 (en) 2002-04-18
EP1349959A2 (en) 2003-10-08

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