WO2002030887A3 - Protein having thrombolytic activities extracted from natural product - Google Patents

Protein having thrombolytic activities extracted from natural product Download PDF

Info

Publication number
WO2002030887A3
WO2002030887A3 PCT/KR2001/001690 KR0101690W WO0230887A3 WO 2002030887 A3 WO2002030887 A3 WO 2002030887A3 KR 0101690 W KR0101690 W KR 0101690W WO 0230887 A3 WO0230887 A3 WO 0230887A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
natural product
thrombolytic
thrombolytic activities
centrifuging
Prior art date
Application number
PCT/KR2001/001690
Other languages
French (fr)
Other versions
WO2002030887A2 (en
Inventor
Young Gil Kwon
Won Dae Kang
Original Assignee
Gennix Co Ltd
Young Gil Kwon
Won Dae Kang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gennix Co Ltd, Young Gil Kwon, Won Dae Kang filed Critical Gennix Co Ltd
Priority to AU2001296046A priority Critical patent/AU2001296046A1/en
Publication of WO2002030887A2 publication Critical patent/WO2002030887A2/en
Publication of WO2002030887A3 publication Critical patent/WO2002030887A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to the thrombolytic protein having about 28 kD of molecular weight produced by the process comprising the steps of : i) extracting the anchovy fermenting product with 35-45 vol% of ethanol; ii) recovering the supernatant after centrifuging said extract; iii) extracting the supernatant with 70 ∩ 80 vol% of ethanol; iv) recovering the precipitate after centrifuging; v) filtering said precipitate using anion exchange column; and vi) filtering and obtaining said protein using Sephadex gel column.
PCT/KR2001/001690 2000-10-09 2001-10-09 Protein having thrombolytic activities extracted from natural product WO2002030887A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001296046A AU2001296046A1 (en) 2000-10-09 2001-10-09 Protein having thrombolytic activities extracted from natural product

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2000-0059174A KR100419451B1 (en) 2000-10-09 2000-10-09 Protein with thrombolytic activities extracted from natural product
KR2000/59174 2000-10-09

Publications (2)

Publication Number Publication Date
WO2002030887A2 WO2002030887A2 (en) 2002-04-18
WO2002030887A3 true WO2002030887A3 (en) 2002-08-08

Family

ID=19692469

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2001/001690 WO2002030887A2 (en) 2000-10-09 2001-10-09 Protein having thrombolytic activities extracted from natural product

Country Status (3)

Country Link
KR (1) KR100419451B1 (en)
AU (1) AU2001296046A1 (en)
WO (1) WO2002030887A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100419451B1 (en) * 2000-10-09 2004-03-11 드림바이오젠 주식회사 Protein with thrombolytic activities extracted from natural product

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01180834A (en) * 1988-01-13 1989-07-18 Nippon Chem Res Kk Thrombolytic agent
JPH09182583A (en) * 1995-12-29 1997-07-15 Chieko Yatagai Enzyme activating prourokinase and collection of the same

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2804036B2 (en) * 1988-01-21 1998-09-24 株式会社東芝 Noise removal circuit
CA2118994A1 (en) * 1993-06-21 1994-12-22 Claude L. Bertin Polyimide-insulated cube package of stacked semiconductor device chips
KR0180103B1 (en) * 1996-03-27 1999-04-01 정영기 Thrombosis dissolution enzyme from bacillus subtilis
KR100189660B1 (en) * 1996-07-10 1999-06-01 공인수 Thrombolysis enzyme from bacillus sp.
KR100229282B1 (en) * 1996-12-28 1999-12-01 박호군 Novel bacillus licheniformis and fibrinolytic enzyme
KR100284758B1 (en) * 1997-11-21 2001-03-15 김철호 Thrombolytic enzyme protein isolated from slugs
KR100319397B1 (en) * 1999-07-12 2002-01-05 남 승 우 Anti-Thrombotic and Anti-Hypercholestemic Composition Which Comprises Plant Extracts as an Active Ingredient
KR100373395B1 (en) * 2000-07-22 2003-02-25 변유량 Novel Thrombolytic Protein and a Process for Preparing the Same
KR100419451B1 (en) * 2000-10-09 2004-03-11 드림바이오젠 주식회사 Protein with thrombolytic activities extracted from natural product

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01180834A (en) * 1988-01-13 1989-07-18 Nippon Chem Res Kk Thrombolytic agent
JPH09182583A (en) * 1995-12-29 1997-07-15 Chieko Yatagai Enzyme activating prourokinase and collection of the same

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 198934, Derwent World Patents Index; AN 1989-246415 *
DATABASE WPI Week 199738, Derwent World Patents Index; AN 1997-409885 *
H. SUMI ET AL.: "A unique strong fibrinolytic enzyme (Katsuwokinase) in Skipjack 'Shiokara' a Japanese traditional fermented food", COMP. BIOCHEM. PHYSIOL., vol. 112B, no. 3, 1995, pages 543 - 547, XP003001556, DOI: doi:10.1016/0305-0491(95)00100-X *
H.-K. KIM ET AL.: "Purification and characterization of a novel fibrinolytic enzyme from bacillus sp. KA38 originated from fermented fish", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 84, no. 4, 1997, pages 307 - 317 *
M. DEMIR ET AL.: "Anticoagulant and antiprotease effects of a novel heparinlike compound from shrimp (panaeus brasiliensis) and its neutralisation by heparinase I", CLIN. APPL. THROMBOSIS/HEMOSTASIS, vol. 7, no. 1, January 2001 (2001-01-01), pages 44 - 52 *
M. FUJITA ET AL.: "Purification and characterization of a strong fibrinolytic enzyme (Nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 197, no. 3, 1993, pages 1340 - 1347, XP024766668, DOI: doi:10.1006/bbrc.1993.2624 *
N. NAKAJIMA ET AL.: "Potent fibrinolytic enzyme from the lysate of Katsuwonus pelamis digestive tract (Shiokara): Purification and characterization", BIOSCI. BIOTECHN. BIOCHEM., vol. 57, no. 9, 1993, pages 1604 - 1605 *
PATENT ABSTRACTS OF JAPAN *
S.-H. KUM ET AL.: "Purification and characterization of subtilisin DJ-4 secreted by bacillus sp. strain DJ-4 screened from Doen-Jang", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 64, no. 8, 2000, pages 1722 - 1725, XP001016304, DOI: doi:10.1271/bbb.64.1722 *
W. KIM ET AL.: "Purification and characterization of a fibrinolytic enzyme produced from bacillus sp. strain CK 11-4 screened from Chungkook-Jang", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 62, no. 7, 1996, pages 2482 - 2488, XP007908743 *

Also Published As

Publication number Publication date
KR20020028257A (en) 2002-04-17
KR100419451B1 (en) 2004-03-11
WO2002030887A2 (en) 2002-04-18
AU2001296046A1 (en) 2002-04-22

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