WO2002029109A3 - Multiplexed differential displacement for nucleic acid determinations - Google Patents

Multiplexed differential displacement for nucleic acid determinations Download PDF

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Publication number
WO2002029109A3
WO2002029109A3 PCT/US2001/031326 US0131326W WO0229109A3 WO 2002029109 A3 WO2002029109 A3 WO 2002029109A3 US 0131326 W US0131326 W US 0131326W WO 0229109 A3 WO0229109 A3 WO 0229109A3
Authority
WO
WIPO (PCT)
Prior art keywords
primer
reagents
nucleic acid
determinations
serve
Prior art date
Application number
PCT/US2001/031326
Other languages
French (fr)
Other versions
WO2002029109A2 (en
Inventor
Sharat Singh
Anita Inamdar
Edwin F Ullman
Liching Cao
David Albagli
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to AU2001296668A priority Critical patent/AU2001296668A1/en
Publication of WO2002029109A2 publication Critical patent/WO2002029109A2/en
Publication of WO2002029109A3 publication Critical patent/WO2002029109A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Multiplexed determinations of large numbers of events are achieved in an accurate and simple manner by using a multitude of primer reagents in combination with different capture reagents that serve for sequestering all the reagents at a single site, followed by independent release of subsets of the primer reagents using differential release conditions. Also included as part of the primer reagents may be identifiers, which serve to identify a particular characteristic. The method is illustrated using primers with sequences for initiation of chain extension that are joined to or serve as a capture sequence, and where the extended primer has an identifier. After extending the primer, the extended primers are sequestered via the capture sequence onto a sequestering agent, sequentially released and the released extended primers analyzed to provide multiplexed determinations. The subject method finds application for nucleic acid sequencing, single nucleotide polymorphism determinations, identification of nucleic acid fragments, and the like.
PCT/US2001/031326 2000-10-05 2001-10-05 Multiplexed differential displacement for nucleic acid determinations WO2002029109A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001296668A AU2001296668A1 (en) 2000-10-05 2001-10-05 Multiplexed differential displacement for nucleic acid determinations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68459000A 2000-10-05 2000-10-05
US09/684,590 2000-10-05

Publications (2)

Publication Number Publication Date
WO2002029109A2 WO2002029109A2 (en) 2002-04-11
WO2002029109A3 true WO2002029109A3 (en) 2003-11-20

Family

ID=24748680

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/031326 WO2002029109A2 (en) 2000-10-05 2001-10-05 Multiplexed differential displacement for nucleic acid determinations

Country Status (2)

Country Link
AU (1) AU2001296668A1 (en)
WO (1) WO2002029109A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9110075B2 (en) 2001-05-21 2015-08-18 Monogram Biosciences, Inc. Compositions for analyzing proteins

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7771929B2 (en) 2000-04-28 2010-08-10 Monogram Biosciences, Inc. Tag library compounds, compositions, kits and methods of use
US20030207300A1 (en) * 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US5514543A (en) * 1992-04-03 1996-05-07 Applied Biosystems, Inc. Method and probe composition for detecting multiple sequences in a single assay
WO2001006008A2 (en) * 1999-07-16 2001-01-25 Aclara Biosciences, Inc. Multiplexed strand displacement for nucleic acid determinations

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US5514543A (en) * 1992-04-03 1996-05-07 Applied Biosystems, Inc. Method and probe composition for detecting multiple sequences in a single assay
WO2001006008A2 (en) * 1999-07-16 2001-01-25 Aclara Biosciences, Inc. Multiplexed strand displacement for nucleic acid determinations

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9110075B2 (en) 2001-05-21 2015-08-18 Monogram Biosciences, Inc. Compositions for analyzing proteins

Also Published As

Publication number Publication date
WO2002029109A2 (en) 2002-04-11
AU2001296668A1 (en) 2002-04-15

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