WO2002029002A3 - Method for generating cloned animals using chromosome shuffling - Google Patents

Method for generating cloned animals using chromosome shuffling Download PDF

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Publication number
WO2002029002A3
WO2002029002A3 PCT/US2001/031216 US0131216W WO0229002A3 WO 2002029002 A3 WO2002029002 A3 WO 2002029002A3 US 0131216 W US0131216 W US 0131216W WO 0229002 A3 WO0229002 A3 WO 0229002A3
Authority
WO
WIPO (PCT)
Prior art keywords
animals
isogenic
chromosome
cloned
autosomally
Prior art date
Application number
PCT/US2001/031216
Other languages
French (fr)
Other versions
WO2002029002A2 (en
Inventor
Robert Lanza
Jose Cibelli
Philip Damiani
Michael D West
Original Assignee
Advanced Cell Tech Inc
Robert Lanza
Jose Cibelli
Philip Damiani
Michael D West
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Cell Tech Inc, Robert Lanza, Jose Cibelli, Philip Damiani, Michael D West filed Critical Advanced Cell Tech Inc
Priority to AU2002213033A priority Critical patent/AU2002213033A1/en
Priority to US10/398,614 priority patent/US20040139489A1/en
Priority to EP01981389A priority patent/EP1334356A4/en
Priority to CA002425100A priority patent/CA2425100A1/en
Publication of WO2002029002A2 publication Critical patent/WO2002029002A2/en
Publication of WO2002029002A3 publication Critical patent/WO2002029002A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with 'autosomally isogenic' meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both 'autosomally' and 'allelically' isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having the undergo nuclear transfer in order to propagate cloned animals.
PCT/US2001/031216 2000-10-06 2001-10-05 Method for generating cloned animals using chromosome shuffling WO2002029002A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002213033A AU2002213033A1 (en) 2000-10-06 2001-10-05 Method for generating cloned animals using chromosome shuffling
US10/398,614 US20040139489A1 (en) 2001-10-05 2001-10-05 Method for generating cloned animals using chromosome shuffling
EP01981389A EP1334356A4 (en) 2000-10-06 2001-10-05 Method for generating cloned animals using chromosome shuffling
CA002425100A CA2425100A1 (en) 2000-10-06 2001-10-05 Method for generating cloned animals using chromosome shuffling

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23801400P 2000-10-06 2000-10-06
US60/238,014 2000-10-06

Publications (2)

Publication Number Publication Date
WO2002029002A2 WO2002029002A2 (en) 2002-04-11
WO2002029002A3 true WO2002029002A3 (en) 2002-09-19

Family

ID=22896126

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/031216 WO2002029002A2 (en) 2000-10-06 2001-10-05 Method for generating cloned animals using chromosome shuffling

Country Status (4)

Country Link
EP (1) EP1334356A4 (en)
AU (1) AU2002213033A1 (en)
CA (1) CA2425100A1 (en)
WO (1) WO2002029002A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10248361A1 (en) * 2002-06-30 2004-01-22 Beisswanger, Roland, Dr. Method for suppressing the male sex in birds, specifically in laying poultry, by inserting a lethal DNA sequence into sex chromosomes that is active only in male embryos
CN114457360B (en) * 2022-02-11 2023-08-25 中国华能集团清洁能源技术研究院有限公司 Diaphragm-free micro-electrolytic tank amplifying equipment, processing method and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270201A (en) * 1988-03-24 1993-12-14 The General Hospital Corporation Artificial chromosome vector
US6077697A (en) * 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5240840A (en) * 1991-04-05 1993-08-31 Regents Of The University Of Michigan Dna superfragment cloning
US6331659B1 (en) * 1998-01-21 2001-12-18 University Of Hawaii Cumulus cells as nuclear donors
NZ550899A (en) * 1999-01-13 2008-07-31 Revivicor Inc Double nuclear transfer method and the production of a non human animal thereby
WO2002077175A2 (en) * 2001-03-23 2002-10-03 Advanced Cell Technology, Inc. Method for generating cloned animals using chromosome shuffling

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270201A (en) * 1988-03-24 1993-12-14 The General Hospital Corporation Artificial chromosome vector
US6077697A (en) * 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1334356A4 *

Also Published As

Publication number Publication date
EP1334356A4 (en) 2004-10-06
CA2425100A1 (en) 2002-04-11
EP1334356A2 (en) 2003-08-13
AU2002213033A1 (en) 2002-04-15
WO2002029002A2 (en) 2002-04-11

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