WO2002027018A3 - Methods for identifying compounds that modulate biofilm activity - Google Patents

Methods for identifying compounds that modulate biofilm activity Download PDF

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Publication number
WO2002027018A3
WO2002027018A3 PCT/US2001/030533 US0130533W WO0227018A3 WO 2002027018 A3 WO2002027018 A3 WO 2002027018A3 US 0130533 W US0130533 W US 0130533W WO 0227018 A3 WO0227018 A3 WO 0227018A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
identifying compounds
biofilm activity
biological activity
supernatant
Prior art date
Application number
PCT/US2001/030533
Other languages
French (fr)
Other versions
WO2002027018A2 (en
Inventor
George A O'toole
David H Blank
Mary Ellen Davey
Nicky C Caiazza
Roberto Kolter
Deborah A Hogan
Original Assignee
Harvard College
Dartmouth College
George A O'toole
David H Blank
Mary Ellen Davey
Nicky C Caiazza
Roberto Kolter
Deborah A Hogan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Dartmouth College, George A O'toole, David H Blank, Mary Ellen Davey, Nicky C Caiazza, Roberto Kolter, Deborah A Hogan filed Critical Harvard College
Priority to AU2001294895A priority Critical patent/AU2001294895A1/en
Publication of WO2002027018A2 publication Critical patent/WO2002027018A2/en
Publication of WO2002027018A3 publication Critical patent/WO2002027018A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention features a novel method for identifying compounds capable of affecting a microbial biological activity, such as biofilm formation, development and dissolution. The method includes: (a) obtaining supernatant from a closed culture system that contains at least one type of microorganism; (b) exposing a target organism to the supernatant or an extract thereof; and (c) measuring the level of the biological activity. Compounds identified by this method are provided, as well as methods for disrupting biofilms and inhibiting or promoting their formation.
PCT/US2001/030533 2000-09-29 2001-09-28 Methods for identifying compounds that modulate biofilm activity WO2002027018A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001294895A AU2001294895A1 (en) 2000-09-29 2001-09-28 Methods for identifying compounds that modulate biofilm activity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US23663900P 2000-09-29 2000-09-29
US67542500A 2000-09-29 2000-09-29
US09/675,425 2000-09-29
US60/236,639 2000-09-29

Publications (2)

Publication Number Publication Date
WO2002027018A2 WO2002027018A2 (en) 2002-04-04
WO2002027018A3 true WO2002027018A3 (en) 2004-02-19

Family

ID=26929966

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/030533 WO2002027018A2 (en) 2000-09-29 2001-09-28 Methods for identifying compounds that modulate biofilm activity

Country Status (2)

Country Link
AU (1) AU2001294895A1 (en)
WO (1) WO2002027018A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102911899B (en) * 2012-10-22 2014-03-12 清华大学深圳研究生院 Proteus mirabilis and application thereof in suppression of biofilm and detoxification

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007518400A (en) * 2003-12-04 2007-07-12 バイオフィルムズ ストラテジーズ， インコーポレイテッド Methods and compositions for preventing biofilm formation, reducing existing biofilm, and reducing bacterial populations
DE102005014805B4 (en) * 2005-03-31 2012-12-06 Bayerische Motoren Werke Aktiengesellschaft Method for determining the growth of environmental germs
WO2007081455A2 (en) * 2005-12-09 2007-07-19 Research Foundation Of State University Of New York Induction of a physiological dispersion response in bacterial cells in a biofilm
WO2007075595A2 (en) * 2005-12-20 2007-07-05 Vertex Pharmacueticals Incorporated Biofilm assay
JP5548121B2 (en) 2007-05-14 2014-07-16 リサーチ ファウンデーション オブ ステイト ユニバーシティ オブ ニューヨーク Induction of physiological dispersive responses in bacterial cells in biofilms
CN102851356B (en) * 2012-03-22 2014-05-14 冯家望 Composite gene chip and method for detection of fourteen common pathogenic bacteria
DK3081650T3 (en) * 2013-12-10 2019-06-03 Hyundai Motor Co Ltd PROCEDURE FOR SCREENING ANTIMICROBYLIC FABRIC
CN113481105B (en) * 2021-07-22 2022-05-17 云南大学 Novel phomopsis fungus strain, preparation method and application
CN114349174B (en) * 2022-01-17 2022-10-04 大连理工大学 Method for removing tetracycline based on algae-bacteria complex

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997033972A1 (en) * 1996-03-13 1997-09-18 University Technologies International Inc. Biofilm incubation
WO1999055368A1 (en) * 1998-04-27 1999-11-04 President And Fellows Of Harvard College Regulation of biofilm formation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997033972A1 (en) * 1996-03-13 1997-09-18 University Technologies International Inc. Biofilm incubation
WO1999055368A1 (en) * 1998-04-27 1999-11-04 President And Fellows Of Harvard College Regulation of biofilm formation

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CERI H ET AL: "The Calgary Biofilm Device: New technology for rapid determination of antibiotic susceptibilities of bacterial biofilms.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 37, no. 6, June 1999 (1999-06-01), pages 1771 - 1776, XP002209694, ISSN: 0095-1137 *
DESAI JITENDRA D ET AL: "Microbial production of surfactants and their commercial potential.", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 61, no. 1, 1997, pages 47 - 64, XP002209696, ISSN: 1092-2172 *
DEZIEL E ET AL: "Mass spectrometry monitoring of rhamnolipids from a growing culture of Pseudomonas aeruginosa strain 57RP", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1485, no. 2-3, 31 May 2000 (2000-05-31), pages 145 - 152, XP004277354 *
LANG S ET AL: "Rhamnose lipids - biosynthesis, microbial production and application potential.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 51, no. 1, January 1999 (1999-01-01), pages 22 - 32, XP002209695, ISSN: 0175-7598 *
SEIDEL V ET AL: "Partially acetylated tri- and tetrarhamnoside dodecanyl ether derivatives from Cleistopholis patens", PHYTOCHEMISTRY, PERGAMON PRESS, GB, vol. 52, no. 3, October 1999 (1999-10-01), pages 465 - 472, XP004291021, ISSN: 0031-9422 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102911899B (en) * 2012-10-22 2014-03-12 清华大学深圳研究生院 Proteus mirabilis and application thereof in suppression of biofilm and detoxification

Also Published As

Publication number Publication date
WO2002027018A2 (en) 2002-04-04
AU2001294895A1 (en) 2002-04-08

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