WO2002024946A3 - Quadruplex dna and duplex probe systems - Google Patents

Quadruplex dna and duplex probe systems Download PDF

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Publication number
WO2002024946A3
WO2002024946A3 PCT/IB2001/001643 IB0101643W WO0224946A3 WO 2002024946 A3 WO2002024946 A3 WO 2002024946A3 IB 0101643 W IB0101643 W IB 0101643W WO 0224946 A3 WO0224946 A3 WO 0224946A3
Authority
WO
WIPO (PCT)
Prior art keywords
strand
nucleobases
sequence
multiplex structure
probe systems
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2001/001643
Other languages
French (fr)
Other versions
WO2002024946A2 (en
Inventor
Jasmine I Daksis
Glen H Erikson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ingeneus Corp
Original Assignee
Ingeneus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Corp filed Critical Ingeneus Corp
Priority to AU2001284350A priority Critical patent/AU2001284350A1/en
Priority to EP01963329A priority patent/EP1319088A2/en
Priority to CN01817230XA priority patent/CN1535318B/en
Priority to CA002422518A priority patent/CA2422518A1/en
Priority to JP2002529538A priority patent/JP2004520815A/en
Publication of WO2002024946A2 publication Critical patent/WO2002024946A2/en
Publication of WO2002024946A3 publication Critical patent/WO2002024946A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A multiplex structure, such as a nucleic acid quadruplex, includes: a first strand containing a first sequence of nucleobases; a second strand containing a second sequence of nucleobases, wherein the second strand is associated with the first strand by Watson-Crick bonding; a third strand containing a third sequence of nucleobases; and a fourth strand containing a fourth sequence of nucleobases, wherein the fourth strand is associated with the second strand and the third strand by Watson-Crick bonding. Formation of the multiplex structure is promoted by monovalent cations (e.g., sodium and potassium), divalent cations, multivalent cations, intercalating agents and/or molecules known to bind within the minor grooves of nucleic acids. The multiplex structure and the process of forming it have diagnostic, therapeutic, prophylactic and nanoengineering applications.
PCT/IB2001/001643 2000-09-19 2001-09-10 Quadruplex dna and duplex probe systems Ceased WO2002024946A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2001284350A AU2001284350A1 (en) 2000-09-19 2001-09-10 Quadruplex dna and duplex probe systems
EP01963329A EP1319088A2 (en) 2000-09-19 2001-09-10 Quadruplex dna and duplex probe systems
CN01817230XA CN1535318B (en) 2000-09-19 2001-09-10 Quadruplex DNA and duplex probe systems
CA002422518A CA2422518A1 (en) 2000-09-19 2001-09-10 Quadruplex dna and duplex probe systems
JP2002529538A JP2004520815A (en) 2000-09-19 2001-09-10 Quadruplex DNA and double-stranded probe system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/664,827 2000-09-19
US09/664,827 US6900300B1 (en) 2000-09-19 2000-09-19 Quadruplex DNA and duplex probe systems

Publications (2)

Publication Number Publication Date
WO2002024946A2 WO2002024946A2 (en) 2002-03-28
WO2002024946A3 true WO2002024946A3 (en) 2003-03-13

Family

ID=24667604

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/001643 Ceased WO2002024946A2 (en) 2000-09-19 2001-09-10 Quadruplex dna and duplex probe systems

Country Status (7)

Country Link
US (1) US6900300B1 (en)
EP (1) EP1319088A2 (en)
JP (1) JP2004520815A (en)
CN (1) CN1535318B (en)
AU (1) AU2001284350A1 (en)
CA (1) CA2422518A1 (en)
WO (1) WO2002024946A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6656692B2 (en) * 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6927027B2 (en) * 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
ATE418870T1 (en) 2003-04-28 2009-01-15 Kraft Foods R & D Inc COFFEE COMPOSITION
JP2007525224A (en) * 2004-02-28 2007-09-06 チャン−ニン ジェイ． ワン Nucleic acid complex
KR20070062575A (en) 2004-09-24 2007-06-15 인제네우스 인크. Genome analysis
WO2006124089A1 (en) * 2005-05-12 2006-11-23 The Arizona Board Of Regents, A Body Corporate Acting On Behalf Of Arizona State University Self-assembled nucleic acid nanoarrays and uses therefor
KR100833492B1 (en) * 2005-12-08 2008-05-29 한국전자통신연구원 Nucleic acid nanostructures and fabrication method thereof
CA3014792A1 (en) * 2016-02-16 2017-08-24 Carnegie Mellon University Compositions for enhancing targeted gene editing and methods of use thereof
CA2954115C (en) * 2016-10-16 2022-04-12 Neil Gordon Ultra-sensitive bioanalyte quantification from self-assembled quadruplex tags
JP7264877B2 (en) * 2017-08-25 2023-04-25 ゾエティス・サービシーズ・エルエルシー Nucleic acid probe and method for immobilizing nucleic acid probe on solid carrier
CN109097491B (en) * 2018-07-26 2021-11-19 湘潭大学 Fluorescent probe based on HIV gene detection
CN109266783B (en) * 2018-07-27 2022-07-22 广东海洋大学 A Molecular Beacon-G Quadruplex Optical Sensor and Its Application in Detection of SV40 Virus
CN117925782B (en) * 2024-01-16 2024-12-20 深圳吉因加医学检验实验室 Composition for nucleic acid hybridization and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000043543A1 (en) * 1999-01-19 2000-07-27 Dade Behring Inc. Detection of differences between polynucleotides

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
US5874555A (en) 1987-10-30 1999-02-23 California Institute Of Technology Triple helices and processes for making same
US5451502A (en) * 1990-05-04 1995-09-19 Oncor, Inc. Restriction amplification assay
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
SE506700C2 (en) 1996-05-31 1998-02-02 Mikael Kubista Probe and Methods for Analysis of Nucleic Acid
US5948897A (en) * 1996-06-14 1999-09-07 Simon Fraser University Method of binding two or more DNA double helices and products formed
US5912332A (en) 1996-07-26 1999-06-15 Hybridon, Inc. Affinity-based purification of oligonucleotides using soluble multimeric oligonucleotides
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
US6312925B1 (en) 1997-05-08 2001-11-06 Epoch Pharmaceuticals, Inc. Methods and compositions to facilitate D-loop formation by oligonucleotides
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6017709A (en) 1998-04-29 2000-01-25 University Of Houston DNA replication templates stabilized by guanine quartets
US6287772B1 (en) 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
US6255469B1 (en) 1998-05-06 2001-07-03 New York University Periodic two and three dimensional nucleic acid structures
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
GB9821989D0 (en) 1998-10-08 1998-12-02 Hybaid Ltd Detection of nucleic acid polymorphism
US6420115B1 (en) * 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000043543A1 (en) * 1999-01-19 2000-07-27 Dade Behring Inc. Detection of differences between polynucleotides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DENG H. AND BRAUNLIN W.H.: "Duplex to quadruplex equilibrium of the self-complementary oligonucleotide d(GGGGCCCC)", BIOPOLYMERS, vol. 35, no. 6, 1995, pages 677 - 681, XP001127483 *
ECKHART L. ET AL.: "Reverse transcription-polymerase chain reaction products of alternatively spliced mRNAs form DNA heteroduplexes and heteroduplex complexes", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 5, 29 January 1999 (1999-01-29), pages 2613 - 2615, XP002224006 *
LISHANSKI A. ET AL.: "Branch migration inhibition in PCR-amplified DNA: homogeneous mutation detection", NUCLEIC ACIDS RESEARCH, vol. 28, no. 9, 1 May 2000 (2000-05-01), pages e42 i - e42 vii, XP002224005 *
See also references of EP1319088A2 *

Also Published As

Publication number Publication date
CN1535318B (en) 2010-10-06
US6900300B1 (en) 2005-05-31
JP2004520815A (en) 2004-07-15
WO2002024946A2 (en) 2002-03-28
CN1535318A (en) 2004-10-06
CA2422518A1 (en) 2002-03-28
EP1319088A2 (en) 2003-06-18
AU2001284350A1 (en) 2002-04-02

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