WO2002024933A3 - Vecteur viraux dotes d'un tropisme tissulaire pour des lymphocytes t et b ainsi que pour des basophiles/mastocytes - Google Patents

Vecteur viraux dotes d'un tropisme tissulaire pour des lymphocytes t et b ainsi que pour des basophiles/mastocytes Download PDF

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Publication number
WO2002024933A3
WO2002024933A3 PCT/EP2001/011086 EP0111086W WO0224933A3 WO 2002024933 A3 WO2002024933 A3 WO 2002024933A3 EP 0111086 W EP0111086 W EP 0111086W WO 0224933 A3 WO0224933 A3 WO 0224933A3
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WO
WIPO (PCT)
Prior art keywords
cells
acid sequence
modified
viral
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/011086
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English (en)
Other versions
WO2002024933A2 (fr
Inventor
Es Helmuth Hendrikus Gerar Van
Zutphen Marlijn Van
Libin Ma
Menzo Jans Emko Havenga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Galapagos Genomics NV
Janssen Vaccines and Prevention BV
Original Assignee
Galapagos Genomics NV
Crucell Holand BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Galapagos Genomics NV, Crucell Holand BV filed Critical Galapagos Genomics NV
Priority to AU2002220567A priority Critical patent/AU2002220567A1/en
Priority to NZ524907A priority patent/NZ524907A/en
Priority to CA002423091A priority patent/CA2423091A1/fr
Priority to EP01985274A priority patent/EP1320622A2/fr
Publication of WO2002024933A2 publication Critical patent/WO2002024933A2/fr
Publication of WO2002024933A3 publication Critical patent/WO2002024933A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10345—Special targeting system for viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6009—Vectors comprising as targeting moiety peptide derived from defined protein from viruses dsDNA viruses
    • C12N2810/6018—Adenoviridae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6045—RNA rev transcr viruses
    • C12N2810/6054—Retroviridae

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Cette invention a trait à un procédé permettant d'introduire une séquence nucléotidique non virale susceptible d'expression dans une cellule possédant un récepteur commun de fixation non universelle, laquelle cellule est issue du groupe constitué par des lymphocytes T, B et des basophiles/mastocytes. Ce procédé consiste à mettre en contact cette cellule avec un vecteur viral comportant une séquence nucléotidique de recombinaison renfermant une séquence pour l'acide nucléique non viral susceptible d'expression et possédant une coque virale modifiée constituée de protéines de coque virale endogènes et d'une protéine de coque virale modifiée contenant une séquence aminoacide adénovirale provenant d'une protéine de sérotype adénoviral à 35 ou 51 fibres, cette séquence adénovirale de la protéine modifiée étant un ligand pour ledit récepteur de fixation. Dans une variante, le vecteur comporte une séquence codant une capside virale constituée de protéines de coque virale endogènes et d'une protéine de capside modifiée contenant une séquence aminoacide adénovirale provenant d'un sérotype adénoviral autre que le sérotype des protéines capsidiques endogènes, cette protéine modifiée étant un ligand pour ledit récepteur de fixation. Cette invention porte également sur un procédé de transduction cellulaire, cette cellule étant choisie dans le groupe constitué par des lymphocytes T, des lymphocytes B et des basophiles/mastocytes. Ce procédé consiste à mettre en contact ces cellules avec une particule adénovirale comportant une séquence nucléotidique non adénovirale et une protéine de capside chimère renfermant une séquence aminoacide dérivée d'au moins deux sérotypes adénoviraux, cette particule ayant un tropisme à l'égard de ces cellules supérieur à au moins l'un des sérotypes adénoviraux comportant ladite protéine de capside chimère. L'invention concerne, de plus, des cellules transduites, des ensembles de sous-populations de cellules, un procédé de transduction ex vivo d'une population cellulaire et une méthode d'administration à un être humain ou à un autre mammifère d'une population de cellules modifiées ex vivo par génie génétique. Elle a trait, en outre, à une technique d'identification de la fonction d'un premier acide nucléique dans des cellules hématopoïétiques.
PCT/EP2001/011086 2000-09-25 2001-09-25 Vecteur viraux dotes d'un tropisme tissulaire pour des lymphocytes t et b ainsi que pour des basophiles/mastocytes Ceased WO2002024933A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002220567A AU2002220567A1 (en) 2000-09-25 2001-09-25 Viral vectors having tissue tropism for T-lymphocytes, B- and mast cells
NZ524907A NZ524907A (en) 2000-09-25 2001-09-25 Adenoviral vectors having tissue tropism for T-lymphocytes, B- and mast cells
CA002423091A CA2423091A1 (fr) 2000-09-25 2001-09-25 Vecteur viraux dotes d'un tropisme tissulaire pour des lymphocytes t et b ainsi que pour des basophiles/mastocytes
EP01985274A EP1320622A2 (fr) 2000-09-25 2001-09-25 Vecteur viraux dotes d'un tropisme tissulaire pour des lymphocytes t et b ainsi que pour des basophiles/mastocytes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP00203375.1 2000-09-25
EP00203375A EP1191105A1 (fr) 2000-09-25 2000-09-25 Vecteurs d'apport de gènes munis d'un tropisme pour les T-lymphocytes
US29040301P 2001-05-11 2001-05-11
US60/290,403 2001-05-11

Publications (2)

Publication Number Publication Date
WO2002024933A2 WO2002024933A2 (fr) 2002-03-28
WO2002024933A3 true WO2002024933A3 (fr) 2002-07-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/011086 Ceased WO2002024933A2 (fr) 2000-09-25 2001-09-25 Vecteur viraux dotes d'un tropisme tissulaire pour des lymphocytes t et b ainsi que pour des basophiles/mastocytes

Country Status (6)

Country Link
EP (2) EP1191105A1 (fr)
AU (1) AU2002220567A1 (fr)
CA (1) CA2423091A1 (fr)
NZ (1) NZ524907A (fr)
WO (1) WO2002024933A2 (fr)
ZA (1) ZA200302124B (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002029073A2 (fr) * 2000-10-06 2002-04-11 Crucell Holland B.V. Vecteurs d'apport de genes pour cellules souches
EP1811031A1 (fr) 2006-01-18 2007-07-25 Millegen Méthode de sélection d'un peptide ou polypeptide capable de se fixer à une molécule cible
CA2658805A1 (fr) * 2006-07-28 2008-01-31 Commonwealth Scientific And Industrial Research Organisation Procedes et compositions permettant d'augmenter le tropisme tissulaire des vecteurs recombinants d'adenovirus
ES3011466T3 (en) 2013-04-18 2025-04-07 Tilt Biotherapeutics Oy Enhanced adoptive cell therapy
US12421502B2 (en) 2013-04-18 2025-09-23 Tilt Biotherapeutics Oy Enhanced adoptive cell therapy
US11155832B2 (en) 2016-09-30 2021-10-26 Genvec, Inc. Adenovectors for delivery of therapeutic genetic material into T cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000042208A1 (fr) * 1999-01-14 2000-07-20 The Scripps Research Institute Vecteurs d'adenovirus, lignees cellulaires d'encapsidation, compositions et procedes de preparation et d'utilisation correspondants

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030017138A1 (en) * 1998-07-08 2003-01-23 Menzo Havenga Chimeric adenoviruses
PT1816205E (pt) * 1999-05-17 2011-11-15 Crucell Holland Bv Adenovírus recombinante baseado no serótipo 48 (ad48)
ATE291633T1 (de) * 1999-06-01 2005-04-15 Univ Washington Rekombinante adenovirale vektoren, die chimäre fiberproteine exprimieren, für die zellspezifische infektion und genomische integration

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000042208A1 (fr) * 1999-01-14 2000-07-20 The Scripps Research Institute Vecteurs d'adenovirus, lignees cellulaires d'encapsidation, compositions et procedes de preparation et d'utilisation correspondants

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
SEGERMAN A. ET AL.: "Adenovirus types 11p and 35p show high binding efficiencies for committed hematopoietic cell lines and are infective to these cell lines.", JOURNAL OF VIROLOGY, vol. 74, no. 3, February 2000 (2000-02-01), pages 1457 - 1467, XP002161682, ISSN: 0022-538X *
SEGGERN VON D. J. ET AL.: "ADENOVIRUS VECTOR PSEUDOTYPING IN FIBER-EXPRESSING CELL LINES: IMPROVED TRANSDUCTION OF EPSTEIN-BARR VIRUS-TRANSFORMED B CELLS", JOURNAL OF VIROLOGY, vol. 74, no. 1, January 2000 (2000-01-01), pages 354 - 362, XP000906911, ISSN: 0022-538X *
SHARMA S. ET AL.: "Efficient infection of a human T-cell line and of human primary peripheral blood leukocytes with a pseudotyped retrovirus vector.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 93, no. 21, 1996, 1996, pages 11842 - 11847, XP002161681, ISSN: 0027-8424 *
SHAYAKHMETOV D. M. ET AL.: "EFFICIENT GENE TRANSFER INTO HUMAN CD34+ CELLS BY A RETARGETED ADENOVIRUS VECTOR", JOURNAL OF VIROLOGY, vol. 74, no. 6, March 2000 (2000-03-01), pages 2567 - 2583, XP000938716, ISSN: 0022-538X *

Also Published As

Publication number Publication date
AU2002220567A1 (en) 2002-04-02
EP1191105A1 (fr) 2002-03-27
WO2002024933A2 (fr) 2002-03-28
EP1320622A2 (fr) 2003-06-25
CA2423091A1 (fr) 2002-03-28
ZA200302124B (en) 2004-03-17
NZ524907A (en) 2004-11-26

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