WO2002024869A2 - Elicitor de origen fungico y proceso para su preparacion - Google Patents
Elicitor de origen fungico y proceso para su preparacion Download PDFInfo
- Publication number
- WO2002024869A2 WO2002024869A2 PCT/IB2001/001850 IB0101850W WO0224869A2 WO 2002024869 A2 WO2002024869 A2 WO 2002024869A2 IB 0101850 W IB0101850 W IB 0101850W WO 0224869 A2 WO0224869 A2 WO 0224869A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- elicitor
- fusarium oxysporum
- systemic resistance
- fraction
- mixture
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/77—Fusarium
Definitions
- the present invention is related to the agriculture-! field.
- the subject matter of the present invention provides a process for preparing a fungal elicitor and a product obtained by means of said process, i.e., an elicitor inductor of systemic resistance in plants, as an alternative to fungicides.
- the plants do not count with a defense system mediated by antibodies as the animais. However, they have constitutive strategies which act as fungistatic or bacteriostatic, delaying the penetration rate of the pathogen. Said constitutive strategies are preformed barriers and a series of enzymes and toxins which affect the pathogen, inhibiting their growth and / or discouraging the attack. Furthermore, the plants rely on active defense mechanisms comprising a complex system of inducible responses, which are manifested through the synthesis of proteins, carbohydrates and secondary metabolites with activities similar to those described above.
- the defense comppunds of the plants can be classified according to their size and mode of action (Kuc, J. 1995. Systemic Induced Resistance as part of integrated plant disease control. In: “Environmental biotic factors in integrated plant disease control” (M. Manka, Ed.). The Polish Phytopathological Society, Poznan. 129-136; Stermer, BA, 1995, Molecular regulation of systemic induced resistance. In: Induced resistance to Disecase in Plants, pp 111-140 (De. Hammershmidt, R. and Kuc, J.), Dodrecht Kluwer).
- the high molecular weight compounds include those functioning as physicochemical barriers, eg, cutin, suberin, lignin, callóse, glycoproteins enriched with hydroxyproline and glycine, phenols crossed-over with wall polysaccharides and pigments.
- a second group of defense compounds of high molecular weight includes enzymes which can act
- glucanase proteases and ribonucleases, and other enzymes that can be generated reactive molecules by
- hydrolithic action such as ⁇ and ⁇ glycosidases
- the second group of defense compounds corresponds to substances with antimicrobial activity and low molecular weight, which can be considered analogous to pesticides. These include compounds formed in the plant prior to infection (eg tannins, sucrose esters and steroids), compounds released or modified as a result of the infection or damage (tannins, glycosides and phenols), compounds (phytoalexins) locally synthesized around the infection or stress site (Kuc, 1995. Phytoalexins, stress metabolism and disease resistance in plants. Ann. Review of Phytopathology. 33: 275- 297).
- the defense mechanisms of plants can be activated in a natural way during contact with the invader. The outcome of which can be beneficial, neutral or detrimental (infection) for the plant.
- the same defense mechanisms can be induced in the plants by means of chemical substances (salicylic acid analogous (BION)), microorganism fractions (virus, bacteria or fungi), attenuated pathogens, extracts from plants and metabolites thereof, among others.
- These substances inducing the process of vegetable defense are known as elicito ⁇ rs (Ebel. J. & Cosió, EG, 1994, Elicitors of plant defense response. Int. Rev. Cytol. 148: 1-36) and the resistance conferred in this way has been defined as induced resistance (Sticher, L., Mauch-Mani, b., Metraux, JP 1997. Systemic Acquired Resistance. Ann. Rev. "Phytopathol.
- SIR Systemic Induced Resistance
- the elicitors of resistance from natural origin, from plants, fungi, bacteria and virus pathogens as well as non pathogens, alive or fractions thereof, are known in the state of the art, as it can be observed, for example, in document WO 00 / 20616; or in document of Rohwer et al. (Rohwer, F., Fritzemeier, KH, Schéel, D., and Halbrock, K. 1987. Biochemical reactions of different tissues of potato ⁇ .Solanum tuberosum) to zoospores or elicitors from Phytophtora infes ans.
- the fundamental action mechanism of the elicitors from the state of th ⁇ art are based in the stimulus of - a particular metabolic route which depends upon the characteristics of the selected elicitor.
- the action mechanisms of the elicitors known in the state of the art are ruled by the vertical resistance theory (gene by gene, Flor, H., 1971, Models of host pathogen interaction in flax rust: its genetics and other simplifications Ann. Rev. P ytopathol, 9: 275-296), which stat s that to each gene (R-dominant) for the host resistance (the plant) corresponds an avirulence gene of the pathogen (P-dominant).
- the gene controlling the recognition of a - signal molecule produced by the pathogen is a resistance gene and the gene of the pathogen controlling the synthesis of the recognized substance (microorga ⁇ ism 's product) has been converted in an avirulence gene.
- the natural or synthetic elicitor is a product homologous of the avirulence gene product.
- the elicitors based in the vertical resistance mechanism provide an alternative for the use of pesticides in the agricultural field, there is a need of supplying new improved elicitors which provide a systemic induced resistance (SRI) in plants.
- SRI systemic induced resistance
- the resistance elicitors from natural origin are produced from plants, fungi, bacteria, and viruses pathogens as well as non pathogens, alive or fractions thereof.
- a fungus of particular relevance is Fusarium oxysporum which, up to the present invention, has not been used in the manufacture of an elicitor.
- Fusarium oxysporum is a cosmopolitan fungus which causes the vascular wilt of manifold species of angiosperms and gymnosperms, among coffee, banana, carnation, tomato, cotton, palm nuts, naranjilla and many others.
- Vascular wilt in tomato by Fusarium was reported by the first time in * L986, and since then, the interest in this disease has continued due to its worldwide distribution.
- the pathogen establishes in the vascular system and with odd exceptions, the pathogen does not leave it but up to the host's death.
- the pathogen generally penetrates through the cell walls of the root tip, although, it can also colonize through the hypocotyl and cotyledons. It oves inter or intracellular through the parenchymatous tissues, invading them and living within the xylem vessels as a saprophyte.
- the effect in the host is the occlusion of the water transportation system (Beckman 1987, The nature of wilt diseases in plants.
- Fusarium oxysporum is a fungus that belongs to the genus deuteromycotina, which includes species whose sexual state (teleomorph) is unknown. Fusarium species have a common characteristic: the production of macroconidia, which can be multinucleate and with different geometrical forms, sizes, colors, and architecture. These characteristics as a whole constitute the basis for subdividing the genus in species.
- Fusarium oxysporum 's control in different crops ranges from the intensive use of chemical pesticides of different origins to soil vaporization.
- the chemical control has not been very successful and the vaporization is highly expensive and it just exterminates the fungus up to 20 centimeters of depth, covering only a period of culture free from the disease.
- Fusarium oxysporum is an unique tool for the production of elicitors in view of its broad spectrum of hosts, its pathogenic mechanism and its omnipresence.
- the first aspect of the invention is provided by a process for preparing an elicitor from the fungus Fusarium oxysporum.
- a second aspect of the invention is provided by a Fusarium oxysporum elicitor, obtained from the above cited process, which stimulates "systemic induced resistance" in the plants over which it is applied.
- an agricultural composition comprising Fusarium oxysporum elicitor together with a süitable agricultural carrier.
- the subject mattef of the present invention which comprises the process for preparing the elicitor, the obtained elicitor prepared from the mycelium of Fusarium oxysporum fungus and the agricultural, composition comprising the said elicitor, has as a main purpose the provision of a biotechnological alternative to the use of chemical pesticides, stimulating in the plants their natural defense mechanisms, in order to control the effect 'of funga1, bacterial and viral pathogens and in some cases, the pests.
- FIG. 1 Examination of the callóse contents in leaves from plants ⁇ Solanum gui toense, Solanum marginatum and Solanum pseudolulo) treated with the elicitor of the present invention.
- mg callose / g material are in axis Y and, the days after inoculation are established in axis X.
- FIG. 3 Examination of ⁇ -1,3 glucanase activity in leaves from plants ⁇ Solanum qui toense, Solanum marginatum and Solanum pseudolulo) treated with the elicitor of the present invention.
- the enzymatic activity is in axis Y and the days after inoculation are established in axis X.
- An unit of enzymatic activity is equal to the quantity
- FIG. 4 Examination of ⁇ -1,3 glucanase activity in roots from plants ⁇ Solanum qui toense, Solanum marginatum and Solanum pseudolulo) treated with the elicitor of the present invention.
- the enzymatic activity is in axis Y and, the days after inoculation are established in axis X.
- An unit of enzymatic activity is equal to the quantity
- FIG. 5 Examination of ⁇ -1,3 glucanase activity in leaves from potato plants ⁇ Solanum tuberosum) treated with the elicitor of the present invention, in comparison to leaves of potato plants over which pesticides were applied, and leaves of potato plants without treatment (control), along a harvest time.
- the enzymatic activity is in axis Y and, the days of culture are established in axis X.
- An unit of enzymatic activity is equal to the quantity of
- FIG. 6 Examination of ⁇ -1,3 glucanase activity in roots from potato plants ⁇ Solanum tuberosum) treated with the elicitor of the present invention, in comparison to roots - of potato plants over which pesticides were applied, and roots of potato plants without treatment (control), along a harvest time.
- the enzymatic activity is in axis Y and, the days of culture are established in axis X.
- An unit of enzymatic activity is equal to the quantity of
- FIG. 8 Examination of peroxidase activity in roots from potato plants ⁇ Solanum tuberosum) treated with the elicitor of the present invention, in comparison to roots of potato plants over which pesticides were applied, and roots of potato plants without treatment (control) , along a harvest time.
- the enzymatic activity is in axis Y and, the days of culture are established in axis X.
- An unit of peroxidase activity is defined as the quantity of
- the present invention provides a process for preparing an elicitor obtained from Fusarium oxysporum, characterized by comprising the steps of:
- step iii mixing fractions A and B obtained in step ii), being fraction A between 10 and 50% of the mixture and fraction B between 90 and 50% of the mixture.
- revirulentation of the fungus can be carried out over naranjilla ⁇ Solanum qui toense), tomato ⁇ Solanum esculentum), and potato ⁇ Solanum tuberosum) plants or other host.
- the culture of the fungus Fusarium oxysporum can be made over solid Czapeck media or its equivalent (eg, PDA (potato dextrose agar), Malt Extract, Sabouraud, or Martin Agar).
- solid Czapeck media or its equivalent eg, PDA (potato dextrose agar), Malt Extract, Sabouraud, or Martin Agar.
- breakage by freezing of the fungus Fusarium oxysporum is carried out using liquid nitrogen or other liquefied gas at temperatures between -40 ° C and - 170 ° C.
- the buffer used for extracting the broken (powdered) fungus can be a phosphate buffer, citrate or ascorbate.
- the sterilization by heat carried out in step (ii) is made in autoclave.
- the alcoholic solvent used in the extraction of the centrifugation residual can be ethanol.
- the basic solution used for resuspending the centrifugation residual can be NaOH between 0.01 and 2N and the acid solution can be HCl between 6 and 12 N.
- fraction A is a 17% of the mixture and fraction B is a 83% of the mixture.
- the present invention supplies the elicitor obtained by means of the above described process, which comprises a mixture of a fraction A and a fraction B obtained from the fungus Fusarium oxysporum in a ratio 1: 5, being fractions A and B corresponding to those obtained in the above described process.
- fraction A contains proteins, among which 70 to 95% are glycoproteins, from which 15 to 20% contain mannose and arabinose residues, and from 80% to 85% contain galactose. Said proteins have anionic characteristics and they are in a molecular weight range from 6,800 Da (1,122 x 10 ⁇ 20 g) to 76,000 Da (1,254 x 10 "" 19 g).
- fraction B is composed by proteins and polysaccharides of the cell wall of Fusarium oxysporum fungus. 60% of the proteins are mannoproteins having anionic characteristics and they are in a molecular weight range from 10,000 Da (1.65 x 10 ⁇ 20 g) to 65,000 Da (1.0725 x 10 ⁇ 19 g). The polysaccharides of said fraction B contain glucans (from 55 to 70% of the
- N-acetyl glucosamine residues from 0.1 to 0.9% of the whole
- complex hemi ⁇ -elluloses of the xyloglucans type from 8 to 13% of the whole
- reducing sugars from 0.1 to 0.9% of the whole
- the elicitor of the present invention is characterized by a contents of reducing sugars and proteins in a ration of 3: 1, respectively.
- the present invention provides an agricultural composition characterized by comprising the Fusarium oxysporum elicitor of the present invention together with an acceptable agricultural carrier.
- the agricultural carrier should not contain ionic or non-ionic detergents.
- the agricultural carrier can be sodium carbonate with a concentration between 30 and 60 mM. Said sodium carbonate helps to the union between the elicitor of the present invention and the foliar surface.
- the agricultural composition of the present invention is characterized by being applied by spraying.
- the elicitor of the present invention is characterized by accelerating the defense metabolism of the plants.
- glycoproteins glycoproteins, mannoproteins, hemicelluloses, reducing sugars, lipids, etc.
- the biodegradability of the product is 12 hours at 18 ° C in diluted form.
- the elicitor of the present invention does not contaminate water or soils.
- the elicitor of the present invention stimulates an increment of populations of beneficial rhizospherical icroorganisms ⁇ Trichoderma ssp. , Gliocladium ssp. and Pseudomonas fluorescens, among others).
- the elicitor of the present invention prepared as it was mentioned above, has a stimulant effect of the defense mechanisms of the plants when it is applied over healthy plants. This inducing effect of Systemic
- Resistance is ruled by the horizontal resistance theory, which stipulates that the resistance of the plants depends upon the collective activity of the defense compounds, its appearance in suitable order and quantity depends upon a multigenic response, expressed with the simultaneous activation of several metabolic routes ( Kuc, J. 1982. Induced Immunity to plant disease. BioScience 32: 854-860). The product, when applied to plants, stimulates among others:
- the elicitor of the present invention is concentrated and therefore, it should be diluted 100 times for its application on the field.
- the final concentration of the elicitor of the present invention is expressed as reducing sugars (3.6 mg / ml) and total proteins (1.2 mg / ml).
- the applications of the product in order to obtain optimal results of pathogen control should be consecutive applications in youthful plants (two days after they germinated), 12 days post germination and each 30 days during the culture cycle.
- the volume of the applications is 3 mi of diluted liquid product. This is valid in the case of plants of transitory crops.
- the volume of application is the same for perennial, but the optimal concentration is the double.
- the application is made by spraying.
- detergents of ionic or non-ionic character which are found in the commercial adjuvants, should be used since they dilute the membrane receptors with protein character.
- the revirulentatibn of the fungus is made placing 20 microliters of a solution of conidia from Fusarium oxysporum (3 x 10 4 / ml) over naranjilla, tomato, potato plants or over other host cultured under complete sterility conditions ⁇ in vi tro). Isolations of the fungus is done starting from the stems of these plants, which cultured on solid Czapeck media or its equivalent (eg PDA (potato dextrose agar), Malt extract, Saboraud, or Martin agar) between 2 and 6 days
- centrifugation residue of fraction A is submitted to maceration with ethanol (95% - ratios of 1: 2 weight volume) and submitted to incubation at 4 ° C from 24 to 72 hours, in order to precip ⁇ tate polysaccharides.
- Ethanol is eliminated by vacuum filtration and the residue is resuspended in NaOH between 0.1 and 2N (ratios 1: 3 weight volume). This mixture is submitted to incubation (92 ° C from 0.5 to 4 hours). The complex polysaccharides are solubilized in sodium hydroxide at high temperatures.
- the above mixture 's pH is adjusted to 6.0 with HC1 12N and centrifuged (between 8,000 r.p.m. (837.8 rad / s) and 10,000 r.p.m. (1,256.6 rad / s) by 50 min). The supernatant of said centrifugation corresponds to fraction B.
- Fraction A is composed by proteins, lipid and polysaccharides form the cytoplasm and from the membrane of the fungus.
- the protein components of this fraction have been partially characterized by affinity chromatography (Sepharose ConA), ion-exchange chromatography (Sephadex G-25) and gel filtration chromatography (seph).
- affinity chromatography Sepharose ConA
- ion-exchange chromatography Sephadex G-25
- gel filtration chromatography gel filtration chromatography
- Said analysis of fraction A show that 90% of the proteins are glycoproteins, 17.5% of them contain mannose and arabinose residues, and the remainder (82.5%) contain galactose residues.
- Said glycoproteins have anionic characteristics and they are in molecular weight ranges from 6,800 Da (1,122 x 10 ⁇ 20 g) to 76,000 Da (1,254 x 10 ⁇ 19 g).
- Fraction B of Fusarium oxysporum mycelium prepared as it was describe in Example 1, is composed by proteins and polysaccharides of the cell wall of the fungus.
- the protein components and polysaccharides of this fraction B were partially characterized by affinity chromatography, by gel filtration chromatography and by spectrophotometric analysis.
- fraction B shows that 60% of the proteins are mannoproteins having anionic characteristics and they are in a molecular weight range from 10,000 Da (1.65 x 10 ⁇ 20 g) to 65,000 Da (1.0725 x 10 "19 g
- the polysaccharides of this fraction B contain glucans (64% of the whole), of which 23% have ⁇ -1,3 bonds, and N-acetyl glucosamine residues (0.5% of the whole), complex hemicelluloses of the xyloglucans type (11% of the whole) and reducing sugars.
- the contents of reducing sugars and proteins of the elicitor of the present invention are found in a ratio which is always maintained in 3: 1, respectively. Said ratio is a characteristic of the fungus strain and of the isolated fractions thereof, with the above described process.
- the preparation of the agricultural composition comprising the elicitor of the present invention is described afterwards.
- the elicitor of the present invention prepared according to the process described in Example 1 is mixed with sodium carbonate in a concentration from 30 to 60 mM, so as to help the union between the elicitor of the present invention and the foliar surface.
- said agricultural composition the use of detergents of ionic or non-ionic character, which are found in the commercial adjuvants, was avoided since these dilute the membrane receptors of proteinaceous character.
- ⁇ -1,3 glucanase activity a hydrolytic enzyme which degrades the cell / walls of fungi and bacteria.
- Peroxidase activity an enzyme which stimulates the - accumulation of lignin and hydroxyproline rich glycoproteins (HPRG) in the plant walls, conforming barriers against fungal and bacteria penetration.
- the purpose of the present assay was to determine the effect of the elicitor of the present invention over the callóse contents in leaves and roots of three species of the genus Solanum Lam ⁇ S. qui toense (susceptible to Fusarium), S. marginatum (resistant to Fusarium) and S. pseudolulo (susceptible to Fusarium)).
- seeds of the three species were sown under sterile conditions (in vitro), and the
- the quantity of ⁇ -1,3 glucan was determined by fluorometry using, the affinity of
- the duration of the effect of the treatment with the product, regarding the accumulation of callóse in the leaves and the roots, is at least 60 days with just one application.
- the purpose of the pr-esent assay was to determine the effect of the elicitor, of the present invention, over
- Said methodology consists of taking an aliquot from the crude extract together with a solution of the specific substrate (oarweed laminarin from Laminaria digi tata), incubating at 37 ° C for 1 hour stopping the reaction with ice and subsequently, measuring the production of reducing sugars from oarweed laminarin using the methodology proposed by Nelson (Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of sugars. Journal of Biological Chemistry. 153: 257-262) and Somogyi (Somogyi, M. 1952. Notes on sugar determination. Journal of Biological Chemistry. 195:
- S. pseudolulo has a continuous increase in its glucanase activity, reaching at the 60th day post treatment the constitutive levéis of S. marginatum.
- the elicitor of the present invention induces the ⁇ -
- the activity of this enzyme, induced by the elicitor of the present invention, is similar in the susceptible planta to the resistant one by the 20th day post treatment in the leaves and in the roots.
- the duration of the 'effect of the treatment with the elicitor of the present invention, regarding the glucanase activity in the leaves and roots, is at least 60 days, with just one application.
- ⁇ -1,3 glucanase activity was quantified following the methodology described by De Tapia et al. and by Nelson
- the potato plants treated with the elicitor of the present invention accumulate this enzyme in their roots with values 70% over those of the control and pesticide treatments, which are maintained along the cultivation period.
- the quantification of peroxidase activity was carried out following the methodology described by Ampomah (Ampomah, S.A.., Ye, XS, Kuc, J., 1995. Detection of several enzymatic activities in leaf prints of cucumber plants. Physiol. Mol. Plant Pathol. 42: 441-454).
- Said methodology consists of a spectrophotometric pursuit of the kinetics reaction of an aliquot from the crude extract of total proteins, together with hydrogen peroxide and guaiacol.
- the defense capability of plants against fungi, bacteria and virus increases, with the synthesis of antifungal and antibacterial compounds.” The duration of the response goes from several weeks to months.
- the elicitor of the present invention stimulates defense responses, systemic (leaves and roots), which are not stimulated by chemical pesticides.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Pest Control & Pesticides (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/380,878 US20040034883A1 (en) | 2000-09-19 | 2001-09-05 | Fungl elicitor and process for its preparation |
BR0114460-0A BR0114460A (pt) | 2000-09-19 | 2001-09-05 | Evocador fúngico e processo para o seu preparo |
EP01972392A EP1321472A2 (en) | 2000-09-19 | 2001-09-05 | Elicitor of fungal origin and method for its preparation |
AU2001292163A AU2001292163A1 (en) | 2000-09-19 | 2001-09-05 | Elicitor of fungal origin and method for its preparation |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CO00-070.959 | 2000-09-19 | ||
CO00070959A CO5280246A1 (es) | 2000-09-19 | 2000-09-19 | Procedimiento de preparacion de un elicitor de origen fungico y el producto derivado de dicho procedimiento |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002024869A2 true WO2002024869A2 (es) | 2002-03-28 |
WO2002024869A3 WO2002024869A3 (es) | 2002-12-27 |
Family
ID=5331336
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2001/001850 WO2002024869A2 (es) | 2000-09-19 | 2001-09-05 | Elicitor de origen fungico y proceso para su preparacion |
Country Status (6)
Country | Link |
---|---|
US (1) | US20040034883A1 (es) |
EP (1) | EP1321472A2 (es) |
AU (1) | AU2001292163A1 (es) |
BR (1) | BR0114460A (es) |
CO (1) | CO5280246A1 (es) |
WO (1) | WO2002024869A2 (es) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012001329A2 (fr) | 2010-07-02 | 2012-01-05 | Centre National De La Recherche Scientifique - Cnrs | Utilisation d'un extrait naturel de marc de raisin pour stimuler les defenses naturelles de plantes |
WO2023118547A1 (en) * | 2021-12-23 | 2023-06-29 | Keygene N.V. | Panama disease resistance in banana |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8563839B2 (en) | 2008-05-06 | 2013-10-22 | New Biology, Inc. | Methods of reducing leaf senescence using methyl dihydrojasmonate |
US8013226B2 (en) | 2008-05-06 | 2011-09-06 | New Biology, Inc. | Methods for reducing leaf senescence using methyl dihydrojasmonate |
CN108374030A (zh) * | 2018-01-18 | 2018-08-07 | 昆明理工大学 | 一种尖孢镰刀菌胞外多糖的制备方法及应用 |
CA3092105A1 (en) * | 2018-02-28 | 2019-09-06 | Locus Agriculture Ip Company, Llc | Microbe-based products for controlling fusarium infections in plants and agricultural products |
-
2000
- 2000-09-19 CO CO00070959A patent/CO5280246A1/es not_active Application Discontinuation
-
2001
- 2001-09-05 BR BR0114460-0A patent/BR0114460A/pt not_active Application Discontinuation
- 2001-09-05 WO PCT/IB2001/001850 patent/WO2002024869A2/es not_active Application Discontinuation
- 2001-09-05 AU AU2001292163A patent/AU2001292163A1/en not_active Abandoned
- 2001-09-05 EP EP01972392A patent/EP1321472A2/en not_active Withdrawn
- 2001-09-05 US US10/380,878 patent/US20040034883A1/en not_active Abandoned
Non-Patent Citations (3)
Title |
---|
GARCIA-MUNIZ NORA ET AL: "Induction of mRNA accumulation corresponding to a gene encoding a cell wall hydroxyproline-rich glycoprotein by fungal elicitors." PLANT MOLECULAR BIOLOGY, vol. 38, no. 4, 1 November 1998 (1998-11-01), pages 623-632, XP002203651 ISSN: 0167-4412 * |
HEREMANS B: "Evidence for a specific elicitor from culture filtrates of Fusarium oxysporum f. sp. Radicis-lycopersici." MEDEDELINGEN FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE, vol. 61, no. 2A, 1996, pages 261-265, XP001073919 1996 * |
TRILLAS M ISABEL ET AL: "Short- and long-term effects of Fusarium oxysporum elicitors on respiration of carnation callus." PLANT PHYSIOLOGY AND BIOCHEMISTRY (MONTROUGE), vol. 33, no. 1, 1995, pages 47-53, XP001073920 ISSN: 0981-9428 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012001329A2 (fr) | 2010-07-02 | 2012-01-05 | Centre National De La Recherche Scientifique - Cnrs | Utilisation d'un extrait naturel de marc de raisin pour stimuler les defenses naturelles de plantes |
WO2023118547A1 (en) * | 2021-12-23 | 2023-06-29 | Keygene N.V. | Panama disease resistance in banana |
Also Published As
Publication number | Publication date |
---|---|
AU2001292163A1 (en) | 2002-04-02 |
CO5280246A1 (es) | 2003-05-30 |
US20040034883A1 (en) | 2004-02-19 |
EP1321472A2 (en) | 2003-06-25 |
WO2002024869A3 (es) | 2002-12-27 |
BR0114460A (pt) | 2004-06-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Pharand et al. | Cytological aspects of compost-mediated induced resistance against Fusarium crown and root rot in tomato | |
Bianciotto et al. | Cellular interactions between arbuscular mycorrhizal fungi and rhizosphere bacteria | |
Haggag | Colonization of exopolysaccharide-producing Paenibacillus polymyxa on peanut roots for enhancing resistance against crown rot disease | |
Mohamed et al. | Defense responses in grapevine leaves against Botrytis cinerea induced by application of a Pythium oligandrum strain or its elicitin, oligandrin, to roots | |
Moricca et al. | Antagonism of the two-needle pine stem rust fungi Cronartium flaccidum and Peridermium pini by Cladosporium tenuissimum in vitro and in planta | |
Bělonožníková et al. | Pythium oligandrum in plant protection and growth promotion: Secretion of hydrolytic enzymes, elicitors and tryptamine as auxin precursor | |
Wang et al. | Study on the biocontrol potential of antifungal peptides produced by Bacillus velezensis against Fusarium solani that infects the passion fruit Passiflora edulis | |
Someya et al. | Induced resistance to rice blast by antagonistic bacterium, Serratia marcescens strain B2 | |
EP2688412A1 (en) | Insecticidal agents and uses thereof | |
US20040034883A1 (en) | Fungl elicitor and process for its preparation | |
JPWO2003020032A1 (ja) | 植物の病害抵抗性誘導組成物およびその製造法 | |
AU659610B2 (en) | Cold tolerant trichoderma | |
Weinberger et al. | Endogenous and exogenous elicitors of a hypersensitive response in Gracilaria conferta (Rhodophyta) | |
JAE-HAN et al. | Quantitative changes of plant defense enzymes in biocontrol of pepper (Capsicium annuum L.) late blight by antagonistic Bacillus subtilis HJ927 | |
Manjunatha et al. | Ampelomyces | |
Shobha et al. | Induction of systemic resistance by rhizobacterial and endophytic fungi against foot rot disease of Piper nigrum L. by increasing enzyme defense activity | |
Won et al. | Biological control of anthracnose fruit rot disease (Colletotrichum spp.) and fruit yield improvement of jujube (Zizyphus jujuba Miller var. inermis Rehder) using Bacillus velezensis CE 100 | |
Jongsareejit et al. | Colletotrichum siamense as a myco-biocontrol agent for management of the tridax daisy (Tridax procumbens) | |
US8932844B2 (en) | Process for stimulating the defense mechanism of a plant using a bacterial extract elicitor | |
Moataza | Destruction of Rhizoctonia solani and Phytophthora capsici causing tomato root-rot by Pseudomonas fluorescences lytic enzymes | |
EP3626815A1 (en) | Strain of microorganism clonostachys rosea f. catenulata as a biofungicide, plant growth stimulant and metabolite producer for agricultural use | |
KR100459066B1 (ko) | 케토미움 글로보숨 f0142 및 이에 의해 생산된 케토비리딘 a 및 b를 이용한 식물병의 생물학적 방제 방법 | |
KR100319135B1 (ko) | 식물병해 길항 미생물 제제 | |
Paparu et al. | Improved colonization of East African highland Musa tissue culture plants by endophytic Fusarium oxysporum | |
da Cruz et al. | Bioactive compounds of ganoderma lucidum activate the defense mechanisms of soybean plants and reduce the severity of powdery mildew |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
AK | Designated states |
Kind code of ref document: A3 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A3 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
WWE | Wipo information: entry into national phase |
Ref document number: 10380878 Country of ref document: US |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2001972392 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 2001972392 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 2001972392 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: JP |