WO2002012526A3 - Materials and methods for delivery and expression of heterologous dna in vertebrate cells - Google Patents

Materials and methods for delivery and expression of heterologous dna in vertebrate cells Download PDF

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Publication number
WO2002012526A3
WO2002012526A3 PCT/US2001/025287 US0125287W WO0212526A3 WO 2002012526 A3 WO2002012526 A3 WO 2002012526A3 US 0125287 W US0125287 W US 0125287W WO 0212526 A3 WO0212526 A3 WO 0212526A3
Authority
WO
WIPO (PCT)
Prior art keywords
delivery
expression
heterologous dna
vectors
recombinant
Prior art date
Application number
PCT/US2001/025287
Other languages
French (fr)
Other versions
WO2002012526A2 (en
Inventor
Richard W Moyer
Yi Li
Alison Louise Bawden
Original Assignee
Univ Florida
Richard W Moyer
Yi Li
Alison Louise Bawden
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/662,254 external-priority patent/US6933145B1/en
Application filed by Univ Florida, Richard W Moyer, Yi Li, Alison Louise Bawden filed Critical Univ Florida
Priority to EP01962121A priority Critical patent/EP1307572A2/en
Priority to AU2001283326A priority patent/AU2001283326A1/en
Publication of WO2002012526A2 publication Critical patent/WO2002012526A2/en
Publication of WO2002012526A3 publication Critical patent/WO2002012526A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The subject invention pertains to recombinant entomopox vectors which are useful for the delivery and stable expression of heterologous DNA in vertebrate cells. Specifically exemplified is a recombinant EPV from amsacta moorei (AmEPV). Because of the capacity of the EPV to incorporate foreign or heterologous DNA sequences, the vectors of the subject invention can be used to deliver DNA inserts that are larger than 10 kb in size. Accordingly, one aspect of the present invention concerns use of the recombinant vectors for delivery and expression of biological useful proteins in gene therapy protocols. In addition, the subject invention concerns novel AmEVP polypeptides and the polynucleotide sequences which encode these polypeptides.
PCT/US2001/025287 2000-08-10 2001-08-10 Materials and methods for delivery and expression of heterologous dna in vertebrate cells WO2002012526A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01962121A EP1307572A2 (en) 2000-08-10 2001-08-10 Materials and methods for delivery and expression of heterologous dna in vertebrate cells
AU2001283326A AU2001283326A1 (en) 2000-08-10 2001-08-10 Materials and methods for delivery and expression of heterologous dna in vertebrate cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22447900P 2000-08-10 2000-08-10
US60/224,479 2000-08-10
US09/662,254 US6933145B1 (en) 1998-05-29 2000-09-14 Materials and methods for delivery and expression of heterologous DNA in vertebrate cells
US09/662,254 2000-09-14

Publications (2)

Publication Number Publication Date
WO2002012526A2 WO2002012526A2 (en) 2002-02-14
WO2002012526A3 true WO2002012526A3 (en) 2003-01-09

Family

ID=26918754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/025287 WO2002012526A2 (en) 2000-08-10 2001-08-10 Materials and methods for delivery and expression of heterologous dna in vertebrate cells

Country Status (3)

Country Link
EP (1) EP1307572A2 (en)
AU (1) AU2001283326A1 (en)
WO (1) WO2002012526A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8450079B2 (en) 2003-10-31 2013-05-28 Immunetics, Inc. Method for detecting bacteria
US7598054B2 (en) 2003-10-31 2009-10-06 Immunetics, Inc. Rapid peptidoglycan-based assay for detection of bacterial contamination of platelets
CN107881155A (en) * 2017-11-21 2018-04-06 上海海洋大学 Express GCRV spike protein VP55 recombinant baculovirus and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935777A (en) * 1991-02-19 1999-08-10 University Of Florida Research Foundation, Inc. Entomopoxvirus expression system
WO1999061069A1 (en) * 1998-05-29 1999-12-02 University Of Florida Materials and methods for delivery and expression of heterologous dna in vertebrate cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935777A (en) * 1991-02-19 1999-08-10 University Of Florida Research Foundation, Inc. Entomopoxvirus expression system
WO1999061069A1 (en) * 1998-05-29 1999-12-02 University Of Florida Materials and methods for delivery and expression of heterologous dna in vertebrate cells

Also Published As

Publication number Publication date
AU2001283326A1 (en) 2002-02-18
EP1307572A2 (en) 2003-05-07
WO2002012526A2 (en) 2002-02-14

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