WO2002012277A3 - Hybrid combinatorial proteins made from reshuffling of differently folded domains - Google Patents

Hybrid combinatorial proteins made from reshuffling of differently folded domains Download PDF

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Publication number
WO2002012277A3
WO2002012277A3 PCT/GB2001/003508 GB0103508W WO0212277A3 WO 2002012277 A3 WO2002012277 A3 WO 2002012277A3 GB 0103508 W GB0103508 W GB 0103508W WO 0212277 A3 WO0212277 A3 WO 0212277A3
Authority
WO
WIPO (PCT)
Prior art keywords
chimaeric
protein
domains
amino acid
reshuffling
Prior art date
Application number
PCT/GB2001/003508
Other languages
French (fr)
Other versions
WO2002012277A2 (en
Inventor
Lutz Riechmann
Greg Winter
Original Assignee
Diversys Ltd
Lutz Riechmann
Greg Winter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0019362A external-priority patent/GB0019362D0/en
Priority claimed from GB0102678A external-priority patent/GB0102678D0/en
Application filed by Diversys Ltd, Lutz Riechmann, Greg Winter filed Critical Diversys Ltd
Priority to AU2001277614A priority Critical patent/AU2001277614A1/en
Publication of WO2002012277A2 publication Critical patent/WO2002012277A2/en
Publication of WO2002012277A3 publication Critical patent/WO2002012277A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a chimaeric folded protein domain comprising two or more sequence segments from parent amino acid sequences that are not homologous, wherein each of said sequence segments, in isolation, shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains; and to a chimaeric folded protein domain comprising two or more sequence segments which share common sequences or sequences from common regions in the protein fold of their parent amino acid sequences, wherein, in isolation, each said sequence segment shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains. The invention specifically relates to the application of said chimaeric protein domains for use in vaccination, wherein said chimaeric protein domains display conformational B cell epitopes of at least one of its parent amino acid sequences.
PCT/GB2001/003508 2000-08-07 2001-08-03 Hybrid combinatorial proteins made from reshuffling of differently folded domains WO2002012277A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001277614A AU2001277614A1 (en) 2000-08-07 2001-08-03 Novel proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0019362.3 2000-08-07
GB0019362A GB0019362D0 (en) 2000-08-07 2000-08-07 Combinational protein Domains
GB0102678.0 2001-02-02
GB0102678A GB0102678D0 (en) 2001-02-02 2001-02-02 Combinatorial protein domains

Publications (2)

Publication Number Publication Date
WO2002012277A2 WO2002012277A2 (en) 2002-02-14
WO2002012277A3 true WO2002012277A3 (en) 2002-05-30

Family

ID=26244808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/003508 WO2002012277A2 (en) 2000-08-07 2001-08-03 Hybrid combinatorial proteins made from reshuffling of differently folded domains

Country Status (2)

Country Link
AU (1) AU2001277614A1 (en)
WO (1) WO2002012277A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103589749A (en) * 2003-09-29 2014-02-19 孟山都技术有限公司 Methods for enhancing stress tolerance in plants

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021914A1 (en) * 1994-02-10 1995-08-17 Pasteur Merieux Serums Et Vaccins Method for preparing a multicombinatorial library of antibody gene expression vectors
WO1997032017A1 (en) * 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Novel method for the identification of nucleic acid sequences encoding two or more interacting (poly)peptides
WO1999058655A2 (en) * 1998-05-13 1999-11-18 Diversys Limited Phage display selection system for folded proteins
WO2001042432A2 (en) * 1999-12-08 2001-06-14 Medical Research Council Modified enzymatic activity through subdomain swaps between related alpha/beta-barrel enzymes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021914A1 (en) * 1994-02-10 1995-08-17 Pasteur Merieux Serums Et Vaccins Method for preparing a multicombinatorial library of antibody gene expression vectors
WO1997032017A1 (en) * 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Novel method for the identification of nucleic acid sequences encoding two or more interacting (poly)peptides
WO1999058655A2 (en) * 1998-05-13 1999-11-18 Diversys Limited Phage display selection system for folded proteins
WO2001042432A2 (en) * 1999-12-08 2001-06-14 Medical Research Council Modified enzymatic activity through subdomain swaps between related alpha/beta-barrel enzymes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
A CRAMERI ET AL.: "DNA shuffling of a family of genes from diverse species accelerates directed evolution", NATURE., vol. 391, 15 January 1998 (1998-01-15), MACMILLAN MAGAZINES., US, pages 288 - 291, XP002178865, ISSN: 0028-0836 *
K-P HOPFNER ET AL.: "New enzyme lineages by subdomain shuffling", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 95, August 1998 (1998-08-01), NATIONAL ACADEMY OF SCIENCE. WASHINGTON., US, pages 9813 - 9818, XP002103693, ISSN: 0027-8424 *
L RIECHMANN & G WINTER: "Novel folded protein domains generated by combinatorial shuffling of polypeptide segments", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 97, no. 18, 29 August 2000 (2000-08-29), NATIONAL ACADEMY OF SCIENCE. WASHINGTON., US, pages 10068 - 10073, XP002178866, ISSN: 0027-8424 *
M FLAJOLET ET AL.: "A genomic approach of the hepatitis C virus generates a protein interaction map", GENE., vol. 242, no. 2, January 2000 (2000-01-01), ELSEVIER BIOMEDICAL PRESS. AMSTERDAM., NL, pages 369 - 379, XP002178862, ISSN: 0378-1119 *
M M ALTAMIRANO ET AL.: "Directed evolution of new catalytic activity using the alpha-beta-barrel scaffold", NATURE., vol. 403, 10 February 2000 (2000-02-10), MACMILLAN MAGAZINES., US, pages 617 - 622, XP002178864, ISSN: 0028-0836 *
M OSTERMEIER ET AL.: "Combinatorial protein engineering by incremental truncation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 96, March 1999 (1999-03-01), NATIONAL ACADEMY OF SCIENCE. WASHINGTON., US, pages 3562 - 3567, XP002178863, ISSN: 0027-8424 *
P J O'BRIEN & D HERSCHLAG: "Catalytic promiscuity and the evolution of new enzymatic activity", CHEMISTRY AND BIOLOGY., vol. 8, April 1999 (1999-04-01), CURRENT BIOLOGY, LONDON, GB, pages R91 - R105, XP000993553, ISSN: 1074-5521 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103589749A (en) * 2003-09-29 2014-02-19 孟山都技术有限公司 Methods for enhancing stress tolerance in plants
CN103589749B (en) * 2003-09-29 2018-11-02 孟山都技术有限公司 Method for enhancing stress tolerance in plants

Also Published As

Publication number Publication date
AU2001277614A1 (en) 2002-02-18
WO2002012277A2 (en) 2002-02-14

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