WO2002010446A3 - Method for determining a partial nucleotide sequence of a nucleic acid molecule - Google Patents

Method for determining a partial nucleotide sequence of a nucleic acid molecule Download PDF

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Publication number
WO2002010446A3
WO2002010446A3 PCT/EP2001/008671 EP0108671W WO0210446A3 WO 2002010446 A3 WO2002010446 A3 WO 2002010446A3 EP 0108671 W EP0108671 W EP 0108671W WO 0210446 A3 WO0210446 A3 WO 0210446A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
determining
acid molecule
nucleotide sequence
partial nucleotide
Prior art date
Application number
PCT/EP2001/008671
Other languages
German (de)
French (fr)
Other versions
WO2002010446A2 (en
Inventor
Eckhard Nordhoff
Christine Luebbert
Original Assignee
Max Planck Gesellschaft
Eckhard Nordhoff
Christine Luebbert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Eckhard Nordhoff, Christine Luebbert filed Critical Max Planck Gesellschaft
Priority to AU2001282031A priority Critical patent/AU2001282031A1/en
Priority to EP01960566A priority patent/EP1356098A2/en
Publication of WO2002010446A2 publication Critical patent/WO2002010446A2/en
Publication of WO2002010446A3 publication Critical patent/WO2002010446A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry

Abstract

The invention relates to a method for determining a partial nucleotide sequence of a nucleic acid molecule, comprising the following steps: (a) incubating a primer which hybridizes to the nucleic acid molecule with multiple copies of the nucleic acid molecule in the presence of a polymerase and a mixture of desoxyribonucleotides; (b) determining the molecular masses of nucleic acid fragments generated by the elongation of the primer in step (a) which succeed each other in size, by means of mass spectrometry; and (c) determining the nucleotide sequence by determining the growth in molecular mass between the nucleic acid fragments which succeed each other in size.
PCT/EP2001/008671 2000-07-27 2001-07-26 Method for determining a partial nucleotide sequence of a nucleic acid molecule WO2002010446A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001282031A AU2001282031A1 (en) 2000-07-27 2001-07-26 Method for determining a partial nucleotide sequence of a nucleic acid molecule
EP01960566A EP1356098A2 (en) 2000-07-27 2001-07-26 Method for determining a partial nucleotide sequence of a nucleic acid molecule

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10036743A DE10036743A1 (en) 2000-07-27 2000-07-27 Method for determining a partial nucleotide sequence of a nucleic acid molecule
DE10036743.7 2000-07-27

Publications (2)

Publication Number Publication Date
WO2002010446A2 WO2002010446A2 (en) 2002-02-07
WO2002010446A3 true WO2002010446A3 (en) 2003-04-24

Family

ID=7650488

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/008671 WO2002010446A2 (en) 2000-07-27 2001-07-26 Method for determining a partial nucleotide sequence of a nucleic acid molecule

Country Status (4)

Country Link
EP (1) EP1356098A2 (en)
AU (1) AU2001282031A1 (en)
DE (1) DE10036743A1 (en)
WO (1) WO2002010446A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPS205802A0 (en) * 2002-05-01 2002-06-06 Bio-Molecular Holdings Pty Limited Improved cycling device and method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998014616A1 (en) * 1996-10-04 1998-04-09 Perseptive Biosystems, Inc. Methods for determining sequence information in polynucleotides using mass spectrometry
US5912118A (en) * 1991-08-02 1999-06-15 Europaisches Laboratorium Fur Molekularbiologie (Embl) Method for sequencing nucleic acids

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
AU717435B2 (en) * 1996-01-23 2000-03-23 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
DE19710166C1 (en) * 1997-03-12 1998-12-10 Bruker Franzen Analytik Gmbh Two-step method of DNA amplification for MALDI-TOF measurements

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5912118A (en) * 1991-08-02 1999-06-15 Europaisches Laboratorium Fur Molekularbiologie (Embl) Method for sequencing nucleic acids
WO1998014616A1 (en) * 1996-10-04 1998-04-09 Perseptive Biosystems, Inc. Methods for determining sequence information in polynucleotides using mass spectrometry

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOOM VAN DEN D ET AL: "COMBINED AMPLIFICATION AND SEQUENCING IN A SINGLE REACTION USING TWO DNA POLYMERASES WITH DIFFERENTIAL INCORPORATION RATES FOR DIDEOXYNUCLEOTIDES", JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, AMSTERDAM, NL, vol. 35, 1997, pages 69 - 79, XP000199154, ISSN: 0165-022X *
CHEN X ET AL: "Stable-isotope-assisted MALDI-TOF mass spectrometry for accurate determination of nucleotide compositions of PCR products.", ANALYTICAL CHEMISTRY. UNITED STATES 1 AUG 1999, vol. 71, no. 15, 1 August 1999 (1999-08-01), pages 3118 - 3125, XP002220366, ISSN: 0003-2700 *
KIRPEKAR FINN ET AL: "DNA sequence analysis by MALDI mass spectrometry.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 11, 1 June 1998 (1998-06-01), pages 2554 - 2559, XP002220365, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
DE10036743A1 (en) 2002-02-07
WO2002010446A2 (en) 2002-02-07
EP1356098A2 (en) 2003-10-29
AU2001282031A1 (en) 2002-02-13

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